Sugar Ratio Research Articles

Metabolic glycan labelling with bio-orthogonal targeting and its potential in drug delivery.

New modes of targeted drug delivery are emerging with promise of enhancing therapeutic efficacy while reducing side effects. This review examines the landscape of metabolic glycan labelling-a technique gaining traction for its potential in specific drug targeting. By exploiting the natural glycan synthetic pathway of monosaccharides, unnatural sugar analogues are incorporated into glycoproteins, allowing for the presentation of unique functional groups on cells. This enables specific targeting using 'clickable' probes with complementary click chemistry functional groups. The selection of sugar analogues and chemical tags are crucial components explored in this review, alongside considerations for cell lines, tissues, and cargo selection. The review discusses non-therapeutic and therapeutic applications of metabolic glycan labelling, as well as its potential beyond labelling of cell surfaces. The review also highlights underexplored areas of metabolic glycan labelling by assessing the limited literature addressing labelling efficiency, turnover rates, the impact of sugar supplements in cell culture, and the critical cell to functionalised sugar ratio. Furthermore, this review delves into the future landscape and goals of metabolic glycan labelling, envisioning its potential in targeted drug delivery.

"The More, the Better?": The Impact of Sugar-to-Protein Molar Ratio in Freeze-Dried Monoclonal Antibody Formulations on Protein Stability.

Lyophilization is widely used to ensure the stability of protein drugs by minimizing chemical and physical degradation in the dry solid state. To this end, proteins are typically formulated with sugars that form an amorphous immobilizing matrix and stabilize hydrogen bonds replacing water molecules. The optimal amount of sugar required and protein stability at low excipient-to-protein molar ratios are not well understood. We investigated this by focusing on the physical stability of formulations, reflecting highly concentrated monoclonal antibody (mAb) lyophilizates at low sucrose to mAb ratios between 25:1 and 360:1. Additionally, the impact of different excipient types, buffer concentrations, and polysorbates was studied. The mAb stability was evaluated over up to three months at 25 and 40 °C. We investigated the "the more, the better" approach regarding excipient usage in protein formulation and the existence of a potential stabilizing threshold. Our findings show efficient monomeric content preservation even at low molar ratios, which could be explained based on the water replacement theory. We identified an exponential correlation between the sucrose to protein molar ratio and aggregate formation and found that there is no molar ratio threshold to achieve minimum stabilization. Sucrose demonstrated the best stabilization effect. Both mannitol, used as a cryoprotectant at low concentrations, and arginine reduced aggregation compared to the pure mAb formulation. The higher ionic strength of 5 mM histidine buffer enhanced protein stability compared to that of 0.1 mM histidine buffer, which was more pronounced at lower molar ratios. The addition of polysorbate 20 contributed an additional interfacial stabilizing effect, complementing the cryoprotective and lyoprotective properties of sucrose. Overall, a model could be developed to optimize the quantity of sugar required for protein stabilization and facilitate a more rational design of protein lyophilizates. The molar ratio of sugar to protein for high-concentration mAb products is limited by the acceptable tonicity, but we showed that sufficient stabilization can be achieved even at low molar ratios.

The potato sugar transporter SWEET1g affects apoplasmic sugar ratio and phloem-mobile tuber- and flower-inducing signals.

The main phloem loader in potato, sucrose transporter StSUT1, is co-expressed with two members of the SWEET gene family: StSWEET11b, a clade III member of SWEET carriers assumed to be involved in sucrose efflux, and StSWEET1g, a clade I member involved in glucose efflux into the apoplast that physically interacts with StSUT1. We investigated the functionality of SWEET carriers via uptake experiments with fluorescent glucose or sucrose analogs. Inhibition or overexpression of StSWEET1g/SlSWEET1e affected tuberization and flowering in transgenic potato plants. Isolation of the apoplasmic fluid by vacuum infiltration centrifugation revealed changes in the apoplasmic hexose composition and mono-to-disaccharide ratio, affecting sink strength. Down-regulation of StSWEET1g expression affected the expression of SP6A, a tuberigen, and miR172 under LD conditions, leading to early flowering and tuberization. A systematic screen for StSWEET1g-interacting protein partners revealed several proteins affecting cell wall integrity and strengthening. StSWEET1g and the main interaction partners were strongly down-regulated during tuber development. We discuss whether StSWEET1g activity might be linked to cell wall remodeling during tuber development and the switch from apoplasmic to symplasmic phloem unloading.

Structure formation mechanism of pectin-soy protein isolate gels: Unraveling the role of peach pectin fractions

This study investigated the macro & micro properties of the composite gels formed by soy protein isolate (SPI) and peach pectin fractions: water-soluble pectin (WSP), chelator-soluble pectin (CSP), and sodium carbonate soluble pectin (NSP). Specially, the interaction between pectin fractions and SPI was studied to explain the formation mechanism of the composite gels. WSP, as a high methoxyl pectin, exhibited rich branching (sugar ratio B = 3.10). CSP, as a low methoxyl pectin, depicted a high linearity. NSP, with low linearity (sugar ratio A = 6.14), contained numerous side chains. Due to the strong interaction between pectin fractions and SPI, the new composites with excellent dense network microstructures were formed, accompanied by increased apparent viscosity, higher G′ and G′′, and reduced particle size. XRD and FT-IR analysis highlighted the modifications in gel structures. SEM-dispersive X-ray spectroscopy observed elemental distribution and framework composition in pectin-SPI gels. Hydrophobic interaction was the most important chemical force in pectin-SPI binding. Molecular docking results indicated that galacturonic acid in pectin bound more strongly to 7S than to 11S, with tighter hydrogen bonds. Notably, WSP-SPI showed the lowest turbidity, indicating enhanced solubility and particle dispersion, which helped prevent aggregation. CSP-SPI demonstrated the highest G′ and G′′, ascribing to the high linearity and abundant carboxyl groups in CSP. NSP-SPI showed the highest apparent viscosity and irregular structure. Overall, the texture properties of pectin-SPI gels were driven by pectin's structure properties, which would provide new and valuable information for texture control in gel formulation.

Fabrication of yeast engineered porous 13X adsorbent layers for CO2 capture

This study investigates a novel environmentally friendly coating technique using yeast fermentation to obtain super porous Zeolite 13X adsorbent layers through freeze-drying technique for CO2 capture applications. Several mass ratios of sugar to yeast were used to control the porosity and pore sizes in the adsorbent layer. A unique peeling process was integrated to obtain foamy, hollow structures to have deeper layer accessibility of adsorbent for adsorption applications. These coated adsorbent layers were experimentally characterized as a function of the mass ratio of sugar to yeast and adsorbent solid fraction. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and Fourier-transform infrared spectroscopy (FT-IR) are used to analyze the coated layers. The void fraction for coated samples is found to vary between 0.48 and 0.56, making the samples applicable for adsorption applications. Adsorption performance experiments are performed to test the coated samples' performance compared to 13X powder. Due to their simplistic fabrication, cost-effectiveness, rapid pore formation, and high adsorption capacity, these porous 13X layers hold potential for large-scale fabrication and CO2 capture applications.

The operating performance of Modified Basalt Fibers (MBF) bio-nest reactor exposed to nano-plastics

Biological nests made of Modified Basalt Fiber (MBF bio-nests) serve as effective carriers for enhancing wastewater treatment. However, little is known about their performance when exposed to nano-plastics. This study investigates the decontamination efficiency and microbial functionality of four types of MBF and traditional Basalt Fibers (BF) as carriers in contact oxidation reactors. Compared to BF, MBF demonstrated superior growth effects and biocompatibility within the bio-nest. Ca-MBF and Mn-MBF bio-nests exhibited the highest and most uniform absorption capacities, respectively, alongside increased secretion of total Extracellular Polymeric Substances (EPS) and higher Protein to Sugar (PN/PS) ratios. In sewage environments, all MBF groups displayed stable performance in removing NH4+-N and COD. Significant removal of TN and TP was notably observed in Mn-MBF treatments. Mn and Ca treatments predominantly influenced the Proteobacteria and Bacteroidetes phyla, crucial for nitrogen and phosphorus removal. Following exposure to nano-plastics, Mn-MBF and Ca-MBF treatments maintained high decontamination efficiency, particularly for TP and COD (48.64 % to 57.78 % and 90.91 % to 92.89 %, respectively). The significant removal of NH4+-N and TP only occurred in Mn-MBF and Ca-MBF treatments, which stimulated the growth of bacteria resistant to nano-plastics. Key genera such as Zoogloea and Meganema were identified as dominant, contributing to organic matter decomposition, EPS secretion, biofilm condensation, and enhanced microbial attachment. The findings underscore the structural stability enhancement of Mn-MBF and Ca-MBF bio-nests in contact oxidation reactors, demonstrating their resilience against nano-plastic pollution.

Impact of olive oil and potassium carbonate pretreatment on physico-chemical properties of sun-dried Sundar Khani raisins (Vitis vinifera L.)

Sundar Khani is native grape variety of Pakistan which has long berry length and significantly rich in taste and sweetness. There is limited literature on pretreatment and drying data for this valuable variety of Grapes. Current study was conducted to investigate the physio-chemical indices of potassium carbonate (K2CO3) and olive oil combined treatments followed by natural sun drying method. The effects of 2 % K2CO3 pretreatments alone and in combination with various amounts of pure olive oil (1 %, 2 %, and 3 %) were studied. The results showed that, physical attributes like raisin length, width, volume, weight and total replication weight increased under distilled water treatment, while, combination of 2 % K2CO3 + 3 % Olive oil significantly reduced the drying time, 2 % K2CO3 + 1 % Olive oil gave higher final yield, rehydration ratio was highest under dH2O treatment and greater decay loss was observed at 2 %K2CO3 + 2 % olive oil. Further, 2 % K2CO3 displayed higher ratios of total soluble solids, sugars and peroxidase enzyme activity as well as titratable acidity, anthocyanins, total phenols, flavonoids were maximum at 2 % K2CO3 + 2 % Olive oil pretreatment. In addition, 2 % K2CO3 + 3 % Olive oil increased the Catalase enzyme activity as well as achieved higher scores in the evaluation of sensory attributes particularly morphological form, color and overall acceptance. This study aimed to investigate the physicochemical indices of Sundar Khani grapes under different pretreatment and sun-drying conditions using K2CO3 and olive oil and it could be suggested that raisin production of Sundar Khani by using above mentioned treatments along with sun drying could have future scope both in export and industrial markets due to its incredible reduction in drying and processing time.

Association between quantity and quality of carbohydrate intake and glaucoma: a cross-sectional study from the NHANES database.

Glaucoma is a public health problem among the worldwide population. Dietary as a modifiable factor have been reported to be associated with glaucoma. This study aimed to explore the association between quantity and quality of carbohydrate (CH) intake and glaucoma among U.S. adults. In this cross-sectional study, data of participants aged ≥ 40years old were extracted from the National Health and Nutrition Examination Survey (NHANES) 2005-2008. CH intake information were obtained by 24-h dietary recall interview. Glaucoma was defined by regraded disc images. Covariates included demographic information, physical examination, laboratory values, complications and nutrients intake. The weighted univariable and multivariate logistic regression models were used to assess the association between the quantity and quality of CH intake and glaucoma. Subgroup analyses based on the history of hypertension were further assessed the association. The weighted population included a total of 4789 participants, of whom 119 (2.48%) had glaucoma. After adjusting for age, adrenal cortical steroids, hypertension, chronic kidney diseases, diabetes and energy intake, high quantity (OR = 1.83, 95%CI: 1.08-3.11) and low quality (OR = 0.44, 95CI%: 0.20-0.98) of CH intake were associated with the higher odds of glaucoma. High quantity of CH intake (OR = 2.06, 95%CI: 1.15-3.69) was associated with the high odds of glaucoma in hypertension, while high quality of CH intake (fiber-to-CH ratio: OR = 0.23, 95%CI: 0.06-0.82; CH-to-fiber and fiber-to-added sugars ratio: OR = 0.10, 95%CI: 0.02-0.53) were associated with the lower odds of glaucoma in participants without hypertension. In NAHNES 2005-2008, higher quantity and lower quality CH intake were associated with the high odds of glaucoma, especially among patients without hypertension. This study provides a theoretical basis for the health management of glaucoma patients from the perspective of dietary intake.

Foliar application of sodium selenite affects the growth, antioxidant system, and fruit quality of strawberry.

Selenium (Se) plays a vital role in various physiological processes in plants and is regarded as an essential micronutrient for human health as well. In this study, sodium selenite solution at 10, 40, 70, and 100 mg·L-1 concentrations was foliar sprayed, and the strawberry plant growth, antioxidant system, and fruit quality with an emphasis on sugar and acid content were assessed. The results showed that 10 mg·L-1 of sodium selenite treatment promoted plant growth, while all the treated concentrations could enhance photosynthesis, the antioxidant system in leaves, the content of Se, and ascorbic acid in fruits. More importantly, 40 mg·L-1 sodium selenite treatment significantly increased fruit weight, total soluble solid, total phenolic content, and anthocyanins, as well as improved the shape index. Furthermore, it decreased the total flavonoid and proanthocyanidin content. Particularly, sodium selenite treatment at 40 and 70 mg·L-1 largely increased the ratio of soluble sugars to titratable acid. The changes of predominant sugars and organic acids during fruit development were further investigated. The sucrose, fructose, and glucose content was upregulated by sodium selenite treatment through upregulating the activities of sucrose phosphate synthase (SPS) and acid invertase, as well as the FaSPS expression. In addition, sodium selenite treatment inhibited the activity of citrate synthase and phosphoenolpyruvate carboxylase, rather than modulating their transcript levels to reduce the citric acid content. This work presented a potentially efficient approach to enhance plant growth and fruit quality and supplement Se in strawberry, providing insights into the mechanism of regulating sugar and acid metabolism by Se.

Mildew invasion: Deciphering its influence on primary metabolites and microbial dynamics in fermented cigar tobacco ecosystems

This study aimed to elucidate the impact of mildew on the quality of fermented cigar tobacco leaves (CTLs) and identify potential biomarkers. Chemical constituents, microbial communities and metabolites in healthy CTLs (HCTLs) and mildewed CTLs (MCTLs) were systematically analyzed. Compared with HCTLs, the moisture, reducing sugar and total nitrogen contents of MCTLs increased, but the levels of starch, protein, total sugar and carbon-nitrogen ratio decreased. Mildew significantly altered flavor profiles, with 31 volatile flavor compounds (VFCs) and 9 free amino acids (FAAs) showing significant differences. Furthermore, the richness and diversity of bacterial and fungal communities decreased with mildew. The core functional microorganisms, including Pseudomonas, Pectobacterium, Aerococcus, Aspergillus, Alternaria, and Colletotrichum were screened. Notably, 2 chemical components (total acid, reducing sugar), 3 VFCs (2-undecanone,6,10-dimethyl, myosmine, 1-(5-methylfuran-2-yl)ethenone), and 4 FAAs (Asp, Ile, Val, Gly) were proposed as biomarkers indicating mildew tendency in fermented CTLs. These findings provide theoretical foundation for optimizing cigar production.

Integrating organic fertilization and soil tillage techniques to enhance the nutritional attributes of “Royal” grapes (Vitis vinifera L. cv.)

AbstractIn the context of global efforts to improve food quality against the backdrop of climate change and increasing demands for food, this study sought to investigate the influence of tillage and fertilization methods on essential nutritional components. Thus, this extensive investigation into the variability of organic acid, sugar, and vitamin content within berries over the years 2020 to 2022 shows the critical influence of agricultural practices on the nutritional quality of grape. Our findings revealed significant fluctuations in the levels of various organic acids, with oxalic and propionic acids seeing a notable increase in 2022, while tartaric, citric, and maleic acids decreased. Similarly, a significant rise in sucrose, glucose, and fructose levels was documented by 2022, alongside a variable trend in vitamin content. The PCA results revealed a clear impact of tillage and organic amendments on the biochemical composition of Royal grapes. Heatmap findings confirmed the results obtained from PCA and showed that certain treatments resulted in high organic acid content, while others resulted in high sugar content. However, disc harrow and olive blackwater 2021 followed by disc harrow and Antep radish 2021 and no‐tillage broccoli 2020 were the best treatments with a balanced distribution of organic acids, soluble sugars, and vitamins among all treatments. On the other hand, chisel and olive blackwater '20 was the treatment with the lowest ratio of organic acids, sugars, and vitamins, followed by chisel and Antep radish 2020. These results show that the concentration of organic acids, soluble sugars, and vitamins in grapes is more affected by the tillage method than the type of organic fertilizer used. To sum up, our study holds valuable implications for viticulture and agriculture at large, highlighting the potential to tailor farming practices for enhanced nutritional outcomes. Such advancements not only promise to elevate the quality of agricultural produce but also contribute to the broader objective of achieving food security and nutritional adequacy, thus offering a roadmap for future research and practice in sustainable agriculture and viticulture.

Sensory and Chemical Characteristics of Seasoning Made from Tempe Gembus Flour with Sugar and Salt Added

Gembus tempeh flour contains high levels of glutamate which can be used as a flavoring. The aim of this research is to determine the sensory and chemical characteristics of the flavoring ingredients of tempe gembus flour. The formulation ingredient for this research product is tempeh gembus from tofu dregs from the Cibuntu tofu making industry. Flour is made by curing tempeh for 3 days at 35°C, then slicing and drying at 70°C for 2 hours, then grinding and sifting using 80 mesh. The formulation was determined through two stages of sensory testing. Phase I sensory testing included the Duo-Trio differentiation test and hedonic test, using two samples of tempeh gembus flour formulation and a commercial MSG product as a control. Preferences are assessed using the parameters of color, aroma, taste and overall rating. Analysis of data from the differentiation test results uses the Chi-square test, while analysis of hedonic test data uses the Mann-Whitney test. The results of the phase I sensory test were used as a control in the phase II sensory test and compared with the sugar-salt addition formulation with a ratio of tempe gembus flour, salt and sugar of 5:3:2. Chemical testing was carried out which included testing water content, salt content and protein content. The test results showed that the sample with added sugar and salt had a water content of 4.6%, a salt content of 22.56%, a protein content of 7.74%, and panelists preferred sensory properties compared to the control.

The Effect of Psychological Stress on The Body's Energy on Blood Sugar Levels

Running leads to change the blood ingredients in different rates. These changes came within the physiological and biochemical conditions that occurred to face the physical exercises effete with the different training doses. Of these changes the sugar –blood ratio which came within the bodies response , and for the low concentration on the physiological and biochemical basis in setting the training programs for the athletes , and for the important role of this issue to get the highest achievements by the athletes as a result of developing their physical and physiological abilities , it became necessary to search and study the effete of running with different energy systems on the sugar ratio in the athletes blood according to scientific basis to get integrated physical characteristics and elevated scientific competence and to develop their physical systems . The experimental method had been used in this study – the equivalent group on a sample of nine runners who had been chosen deliberately and the researcher used the suitable statistical methods to deal with the results , and throughout these results the researcher get the following conclusions ;-1-There are significance differences between the before and after training testing for the second group of the (1500 m) runners , and the third group of the (5000m) runners , and the advantage is for the other training test relating their sugar – blood ratio .2- There are no significance differences for the first group of the ( 100m ) runners relating their sugar –blood ratio .

Comparing levoglucosan and mannosan ratios in sediments and corresponding aerosols from recent Australian fires

The monosaccharide anhydrides levoglucosan, mannosan, and galactosan are known as ‘fire sugars’ as they are powerful proxies used to trace fire events. Despite their increasing use, their application is not completely understood, especially in the context of tracing past fire events using sediment samples. There are many uncertainties about fire sugar formation, partitioning, transport, complexation, and stability along all stages of the source-to-sink pathway. While these uncertainties exist, the efficacy of fire sugars as fire tracers remains limited. This study compared high-resolution fire sugar fluxes in freshwater sediment cores to known fire records in Tasmania, Australia. Past fire events correlated with fire sugar flux increases down-core, with the magnitude of the flux inversely proportional to the distance of the fires from the study site. For the first time, fire sugar ratios (levoglucosan/mannosan, L/M) in aerosols were compared with those in sediments from the same time-period. The L/M ratio in surface sediments (1.42–2.58) were significantly lower than in corresponding aerosols (5.08–15.62). We propose two hypotheses that may explain the lower average L/M of sediments. Firstly, the degradation rate of levoglucosan is higher than mannosan in the water column, sediment-water interface, and/or sediment. Secondly, the L/M ratio of non-atmospheric emissions during fires may be lower than that of atmospheric emissions from the same fire. Due to the uncertainties about transport partitioning (atmospheric versus non-atmospheric emissions) and fire sugar degradation along all stages of the source-to-sink pathway, we advise caution when inferring vegetation type (e.g. softwood, hardwood, or grasses) based purely on fire sugar ratios in sediments (e.g. L/M ratio). Future investigations are required to increase the efficacy of fire sugars as a complimentary, or standalone, fire tracer in sediments.

Physio-chemical and Phytochemical Characteristics of a Lesser-known Wild Edible Fruit of Mizoram, Elaeagnus pyriformis Hook. F. (Family Elaeagnaceae): Need for its Proper Domestication

The lesser-known yet incredibly nourishing wild edible fruit Elaeagnus pyriformis Hook.f. is found in the north-eastern mountainous states of India. Elaeagnus pyriformis fruits were collected from three villages in Champhai District, Mizoram, with the goal of analysing their physio-chemical and phytochemical qualities in order to determine its potential for domestication. Measurements were made of the fruit's physical characteristics, including its length, diameter, weight, and moisture content. Juice was extracted, and total soluble solids (TSS), titrable acidity, ascorbic acid, reducing sugar, total sugar, non-reducing sugar, and sugar:acid ratio were among the chemical parameters that were examined afterward. The existence of several beneficial substances was determined by phytochemical screening. Physical and chemical differences between the samples from various villages were found to be significantly varied, according to the results. Ruantlang produced the highest titrable acidity, whereas Zotlang had the maximum fruit weight, length, TSS, reducing sugar, total sugar, non-reducing sugar, and flavonoid content. Fruit diameter, the ratio of sugar to acid, and the total phenolic content were all highest in Hmunhmeltha. The fruit's phytochemical examination identified flavonoids, terpenoids, alkaloids, tannins, and cardiac glycosides.

Structural Analyses of Polysaccharides Extracted from Cyanobacterial Extracellular Gels and Oriented Liquid Crystalline Microfiber Processing by Poly(vinyl alcohol)-Assisted Electrospinning.

Sacran is a supergiant cyanobacterial polysaccharide that forms mesogenic supercoil rods that exhibit liquid crystalline (LC) gels at deficient concentrations of around 0.5 wt%, and has several bioactive stimuli-responsive functions. Here, we attempted to form oriented microfibers of sacran by electrospinning, following structural analyses of the sacran rods. A heterogeneous acid-hydrolysis method using a protonated cation-exchange resin was adopted to examine the short-time exposition of concentrated acid to sacran rods. From the supernatant, the oligomeric fraction that was soluble in water and methanol was isolated. The oligomeric fraction had a main sugar ratio of α-Glc:β-Glc:α-Xyl:β-Xyl:α-Rha of 2:5:1.5:1.5:4 (Glc:Xyl:Rha = 7 (=4 + 3):3:4), and it was speculated that the sacran structure includes rhamnoglucan and xyloglucan (4:3), which are generally rigid enough to exhibit LC. To make oriented microfibers of LC sacran, solubility testing was performed on sacran to find good new solvents of polyhydroxy alcohols such as ethylene glycol, 1,2-propanediol, and glycerol. The oriented film was prepared from a sacran aqueous solution where calcium compound particles deposited on the film are different from polyhydroxy alcohol solutions. Although sacran could not form microfibers by itself, polymer composite microfibers of sacran with poly(vinyl alcohol) were prepared by electrospinning. Cross-polarizing microscopy revealed the molecular orientation of the microfibers.

Characterization of physical and chemical properties of honey from Northeastern Anatolia of Türkiye

The aim of this study was to investigate the physical and chemical properties of natural highland honey produced in the northeastern Anatolia region of Turkey. In 2020, 24 honey samples collected and sold during the honey harvest season were purchased from local vendors. Moisture, color, acidity, pH, conductivity, diastase and invertase activity, C13, C13 protein-honey, C4 analysis and sugar components were analyzed in honey samples. The honey samples were found to contain 17.4% moisture. The mean invertase level were found to be 156.216 U/kg. The freshness and enzymatic activity of the honey were shown by the average diastase number of 12.8 DS. The mineral content and overall purity of the honey was indicated by an electrical conductivity value of 0.17 mS/cm. The average acidity value 15.9 meq/kg, fructose/glucose ratio 1.26% and the average color value 33 mm Pfund determined. The average sugar contents in the honey samples were as follows: fructose 37.4%, glucose 29.5%, sucrose 1.4%, turanose 1.8%, maltose 0.6%, isomaltose 0.2%, erlose 0.3%, trehalose 0% hybriditose 0% maltotriose 0% fructose+glucose. 66.9%, fructose/glucose 1.26, glucose/water 2.0. The mean difference between honey protein and honey delta C13 data is -0.58 and C4 sugar ratio is 0. In conclusion, valuable findings were obtained on the physical and chemical properties of honey from the northeastern Anatolia region of Turkey. Further research can build on these findings to explore the unique properties and potential benefits of honey from this region and contribute to its value and utilization in various industries.

Determination of Yield and Quality Characteristics of Some Fodder Beet (Beta vulgaris L. var. rapa) Varieties in Sakarya Ecological Conditions

This research; it was established under the Sakarya ecological conditions and carried out for 2 years between 2021 and 2023 to determine the yield and quality characteristics of some fodder beet varieties. The experiment was set up with four replications in a randomized blocks trial design. Rekord, Rota, Ursus and Zentaur varieties were used in this research. In the study; in tubers; length (cm), diameter (cm), aboveground length ratio (%), yield (kg/da), dry matter content (%), dry matter yield (kg/da), crude protein ratio (%), sugar ratio (%) and weight loss in storage (%) and in leaves; yield (kg/da), length (cm), width (cm), dry matter ratio (%), dry matter yield (kg/da) and crude protein ratio (%) properties were investigated. The most positive data in the study were obtained from the Ursus variety (In tuber: length; 29.1 cm, yield; 19.309 kg/da, dry matter content; 15.9%, crude protein ratio; 9.30%, sugar ratio; 6.35%, and in leaves; length; 61.0 cm, yield; 2.585 kg/da, dry matter content; 14.0%, crude protein ratio; 23.5%).

Nectar Production and Three Main Sugars in Nectar of Salvia pratensis and Salvia glutinosa in Correlation with Abiotic Factors

Floral nectar is mainly a reward in the form of food for pollinators. Its composition plays an important role when pollinators choose their food. Several studies have shown that the popularity of flowers with nectar is influenced by the concentration and ratio of sugars. Here, we present the nectar chemical composition with regard to three main sugars and their concentrations in correlation with abiotic factors for the plant species Salvia pratensis L. and Salvia glutinosa L. through their 2023 flowering season. We sampled nectar using microcapillaries at three different times during the day on sites in nature. Our results show that nectar production in both species is the highest at around 12 a.m. The abiotic factor that affects nectar production in both species is the soil temperature, while UVB radiation does not influence nectar production. Air temperature and air humidity affect the nectar production of S. glutinosa, while soil humidity affects nectar production in S. pratensis. The most represented sugar in S. glutinosa nectar is sucrose, while S. pratensis nectar has more glucose and fructose. Our results show that UVB radiation has an effect on the sucrose level, although it does not have any direct effect on nectar productivity.

Optimization of poly(3-hydroxybutyrate) production with simulating corn stover hydrolysates by Paraburkholderia sacchari

In this study, poly(3-hydroxybutyrate) production by Paraburkholderia sacchari was investigated using glucose-xylose mixtures as simulated corn stover hydrolysates to optimize the culture media. Box-Behnken Design (BBD) was selected to comprehensively understand the effects of four variables including sugar concentration, molar C/N ratio, phosphorus concentration, and sugar ratio on P(3HB) production. The optimized parameters were identified as initial sugar concentration (23 g/L), molar C/N ratio (44), phosphorus concentration (8.7 g/L), and sugar ratio (1.9∶1). Under the optimal conditions, P. sacchari achieved the maximum P(3HB) accumulation of 55.8 % with a P(3HB) titer of 5.16 g/L (0.23 g/g Sugar). The extracted polymer was confirmed as P(3HB) and exhibited enhanced thermostability. Furthermore, P(3HB) was successfully synthesized from ball-milled corn stover hydrolysate under optimized conditions with the P(3HB) titer reached 7.66 g/L (0.33 g/g Sugar), which was higher than the pure mixed sugar system and other reports on lignocellulose hydrolysates with batch fermentation (<5 g/L). Therefore, P. sacchari exhibits the potential for P(3HB) production using corn stover hydrolysates as an inexpensive substrate.