Abstract
In this study, poly(3-hydroxybutyrate) production by Paraburkholderia sacchari was investigated using glucose-xylose mixtures as simulated corn stover hydrolysates to optimize the culture media. Box-Behnken Design (BBD) was selected to comprehensively understand the effects of four variables including sugar concentration, molar C/N ratio, phosphorus concentration, and sugar ratio on P(3HB) production. The optimized parameters were identified as initial sugar concentration (23 g/L), molar C/N ratio (44), phosphorus concentration (8.7 g/L), and sugar ratio (1.9∶1). Under the optimal conditions, P. sacchari achieved the maximum P(3HB) accumulation of 55.8 % with a P(3HB) titer of 5.16 g/L (0.23 g/g Sugar). The extracted polymer was confirmed as P(3HB) and exhibited enhanced thermostability. Furthermore, P(3HB) was successfully synthesized from ball-milled corn stover hydrolysate under optimized conditions with the P(3HB) titer reached 7.66 g/L (0.33 g/g Sugar), which was higher than the pure mixed sugar system and other reports on lignocellulose hydrolysates with batch fermentation (<5 g/L). Therefore, P. sacchari exhibits the potential for P(3HB) production using corn stover hydrolysates as an inexpensive substrate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
