Rates Of Glyphosate Research Articles

Assessing forage bermudagrass cultivar tolerance to glyphosate application

AbstractIn‐season weed control with glyphosate could be a feasible option if bermudagrass [Cynodon dactylon (L.) Pers.] tolerance to glyphosate was better understood. The objective of this study was to evaluate the tolerance of bermudagrass cultivars ‘Goodwell’ and ‘Greenfield’ to varying glyphosate rates under controlled environment. The experimental design was a factorial arrangement in a completely randomized design (CRD) with the two forage bermudagrass cultivars and the five glyphosate application rates (0.35, 0.47, 0.95, 1.37, and 2.75 lb a.i. ac−1) plus the nontreated control. Visual injury (VI) was collected at 8, 16, and 24 days after glyphosate application (DAG), and dry matter regrowth relative to nontreated control (%DMRrel) was measured at 56 DAG. Results indicated that VI developed faster in Goodwell until 8 DAG, but both cultivars had similar glyphosate VI at its peak (16 DAG). Although glyphosate VI was similar in both cultivars at 16 and 24 DAG, Goodwell showed greater %DMRrel at glyphosate rates ≥0.95 lb a.i. ac−1 than Greenfield at 56 DAG. Moreover, the prediction of %DMRrel from previous canopy VI followed distinct negative exponential relationships for each cultivar‐DAG combination. The Akaike Information Criterion (AICc) numerical differences were negligible when comparing relationships at different DAGs for a specific cultivar. Practically, VI readings taken at any evaluated DAGs were considered appropriate for estimating %DMRrel. Results suggested that bermudagrass glyphosate tolerance is cultivar‐dependent, where Goodwell showed improved tolerance allowing in‐season glyphosate application to control weeds at higher rates than Greenfield. Studies at field conditions must be performed to validate our findings.

Evolution of Target-Site Resistance to Glyphosate in an Amaranthus palmeri Population from Argentina and Its Expression at Different Plant Growth Temperatures.

The mechanism and expression of resistance to glyphosate at different plant growing temperatures was investigated in an Amaranthus palmeri population (VM1) from a soybean field in Vicuña Mackenna, Cordoba, Argentina. Resistance was not due to reduced glyphosate translocation to the meristem or to EPSPS duplication, as reported for most US samples. In contrast, a proline 106 to serine target-site mutation acting additively with EPSPS over-expression (1.8-fold increase) was respectively a major and minor contributor to glyphosate resistance in VM1. Resistance indices based on LD50 values generated using progenies from a cross between 52 PS106 VM1 individuals were estimated at 7.1 for homozygous SS106 and 4.3 for heterozygous PS106 compared with homozygous wild PP106 plants grown at a medium temperature of 24 °C day/18 °C night. A larger proportion of wild and mutant progenies survived a single commonly employed glyphosate rate when maintained at 30 °C day/26 °C night compared with 20 °C day/16 night in a subsequent experiment. Interestingly, the P106S mutation was not identified in any of the 920 plants analysed from 115 US populations, thereby potentially reflecting the difference in A. palmeri control practices in Argentina and USA.

Investigations of the sensitivity of ornamental, fruit, and nut plant species to driftable rates of 2,4-D and dicamba

AbstractAn experiment was conducted in 2017 and 2018 to determine the sensitivity of driftable rates of 2,4-D and dicamba with or without glyphosate on common ornamental, fruit, and nut species. Three driftable rates corresponding to ½, 1/20th, and 1/200th of the manufacturer’s labeled rate (1 × rate) of 2,4-D (1.09 kg ae ha−1), 2,4-D plus glyphosate (1.09 kg ae ha−1 plus 1.10 kg ae ha−1), dicamba (0.56 kg ae ha−1), and dicamba plus glyphosate (0.56 kg ae ha−1 plus 1.10 kg ae ha−1) were applied to apple, crabapple, dogwood, American elderberry, American elm, grapevine, hydrangea, red maple, pin oak, peach, pecan, eastern redbud, rose, red raspberry, strawberry, sweetgum, nannyberry viburnum, and black walnut plants. Visible estimates of injury were recorded 28 and 56 days after treatment (DAT). Plant measurements included leaf malformation, tree trunk growth, and shoot length. Across all species, the ½ × rate of 2,4-D plus glyphosate resulted in 61% injury 28 DAT, whereas the ½ × rate of dicamba plus glyphosate resulted in 51% injury. Across plant species and herbicides, ½ ×, 1/20 ×, and 1/200 × rates caused injury ranging from 3% to 100%, 0% to 66%, and 0% to 19%, respectively. Hydrangea was the least sensitive species; grapevine was most sensitive. Changes in plant measurements were dependent on the species and herbicide applied. Treatments at the ½ × or 1/20 × rate resulted in shoot length, leaf malformation, and trunk tree diameter differences for 11, 10, and 7 species, respectively, compared with nontreated plants. Collectively, the measurements and visual injury assessments indicated apple, red maple, peach, and pin oak were more sensitive to treatments containing dicamba, whereas black walnut, grapevine, and American elm were more sensitive to 2,4-D. Although the 1/200 × rates of 2,4-D and dicamba did not result in changes to plant measurements, obvious injury symptoms were observed, which could render these plants unsalable.

Tank contamination with dicamba and 2,4-D influences dry edible bean

AbstractThe occurrence of herbicide tank contamination with dicamba or 2,4-D will likely increase with the recent commercialization of dicamba- and 2,4-D-resistant soybean. High-value sensitive crops, including dry bean, will be at higher risks for exposure. In 2017 and 2018, two separate field experiments were conducted in Michigan to understand how multiple factors may influence dry bean response to dicamba and 2,4-D herbicides, including 1) the interaction between herbicides applied POST to dry bean and dicamba or 2,4-D, and 2) the impact of low rates of glyphosate with dicamba or 2,4-D. Dry bean injury was 20% and 2% from POST applications of dicamba (5.6 h ae ha−1) and 2,4-D (11.2 g ae ha−1), respectively, 14 days after treatment (DAT). The addition of glyphosate (8.4 g ae ha−1) did not increase dry bean injury from dicamba or 2,4-D. Over 2 site-years the addition of dry bean herbicides to dicamba or dicamba + glyphosate (8.4 g ae ha−1) increased dry bean injury and reduced yield by 6% to 10% more than when dicamba or dicamba + glyphosate was applied alone. The interaction between 2,4-D (11.2 g ae ha−1) and dry bean herbicides was determined to be synergistic. However, 2,4-D (11.2 g ae ha−1) had little effect on dry bean with or without the addition of a dry bean herbicide program. These studies document that synergy also occurs between dicamba and dicamba + glyphosate and both common dry bean herbicide programs tested: 1) imazamox (35 g ha−1) + bentazon (560 g ha−1), and 2) fomesafen (280 g ha−1). The synergy between dry bean herbicide and dicamba and dicamba + glyphosate can increase plant injury, delay maturity, and reduce yield to a greater extent than dicamba or dicamba + glyphosate alone. This work emphasizes the need to properly clean out sprayers after applications of dicamba to reduce the risk of exposure to other crops.

INTERFERÊNCIA DO ESTÁDIO FENOLÓGICO DO CAPIM-AMARGOSO SOBRE A EFICÁCIA DO HERBICIDA GLYPHOSATE

Considera-se que o controle eficiente de plantas daninhas com o uso de herbicidas em pósemergência depende, sobretudo, do estádio de desenvolvimento das plantas alvo no momento da aplicação. Neste sentido, o estádio fenológico das plantas é uma variável importante a ser considerada no ambiente de produção, pois interfere na eficácia do produto e exige readequação das doses recomendadas. Assim sendo, este trabalho foi desenvolvido com o objetivo de avaliar a suscetibilidade do capim-amargoso ao herbicida glyphosate influenciada pelo estádio fenológico no momento da aplicação. Para tanto, dois experimentos semelhantes foram realizados, nos quais os tratamentos foram organizados segundo esquema fatorial 8x5, em que oito foram as doses do herbicida glyphosate (0, 45, 90, 180, 360, 720, 1440, 2880 g ha-1) e cinco os estádios fenológicos do capim-amargoso. Comparado à variável massa seca, observou-se que a variável controle percentual promoveu melhor estimativa da suscetibilidade do capim-amargoso ao glyphosate, considerando-se diferentes estádios fenológicos; para esta variável o cálculo de DL80 resultou em excelente estimativa de suscetibilidade da espécie, obtendo-se a equação y = 26,276x – 112,42. Ou seja, a cada 10 unidades na escala BBCH, necessita-se de 150 g ha-1 adicionais de glyphosate para obter o mesmo DL80.

Non–2,4-D–resistant cotton response to glyphosate plus 2,4-D choline tank contamination

AbstractField trials were conducted near Lubbock, TX, in 2013, 2014, and 2015 to evaluate non–2,4-D–resistant cotton response to low rates of glyphosate plus 2,4-D choline. Cotton was treated with five rates of glyphosate plus 2,4-D choline (0.0183, 0.183, 1.83, 18.3, and 183 g ae ha−1) at two application timings (nine leaf and first bloom). These rates correspond to contamination rates of 0.0008%, 0.008%, 0.08%, 0.8%, and 8%, respectively. Visual cotton injury, boll retention, lint yield, and fiber properties were recorded. When averaged over contamination rates, visual injury after applications made to nine-leaf cotton was greater than for first-bloom cotton in three of 3 yr and yield loss was greater when applications were made to nine-leaf cotton when compared with first-bloom cotton in two of 3 yr. Averaged over application timing, lint yield in 2013, 2014, and 2015 after glyphosate plus 2,4-D choline contamination rates of 0.0008% and 0.008% were not different than that of the nontreated control, whereas contamination rates of 0.08%, 0.8%, and 8% decreased yield by 3% to 20%, 45% to 58%, and 80% to 96%, respectively. Contamination rates of 0.0008%, 0.008%, 0.08%, and 0.8% rarely affected fiber quality; however, a contamination rate of 8% frequently decreased micronaire, fiber length, fiber length uniformity, and fiber strength. This decrease in fiber quality also resulted in a reduction in cotton loan value and potential financial return. Although decreases in fiber quality parameters were not observed with the 0.8% contamination rate, significant reductions in financial return occurred due to yield loss caused by injury from glyphosate plus 2,4-D choline.

Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards.

Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, and picloram, which showed poor control (<50%). In in vivo assays, resistance levels were high for glyphosate and moderate for picloram, dicamba, and 2,4-D. Sequencing the 5-enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser amino acid substitution, conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAHs (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports, for the first time, multiple resistance to SAHs and glyphosate in P. hysterophorus.

Chitosan/glyphosate formulation as a potential, environmental friendly herbicide with prolonged activity

Although there are many reports on the dangers posed by glyphosate, this herbicide is still one of the most popular and widely used total weed killers. Therefore, great effort should be made to minimize exposure to this herbicide and limit its losses into the environment. The aim of this study was to prepare a new formulation consisted of various molecular weight chitosans with glyphosate and their evaluation toward active substance release, phytotoxicity, and preliminary herbicidal efficiency. The phytotoxicity study of the obtained chitosan/glyphosate formulations was determined based on the Organisation for Economic Co-operation and Development 208 guideline. Among all tested formulations evaluated for phytotoxicity, that containing the highest molecular weight of chitosan was found to be the least toxic, showing simultaneously the most effective herbicidal activity against selected weeds. The release rate of glyphosate from the obtained formulations was dependent on the molecular weight and viscosity of chitosan.

Controle e susceptibilidade de capim-braquiária e capim-ruziziensis ao glyphosate e fluazifop-p-butil

Este estudo teve como objetivo avaliar o controle e susceptibilidade de capim-braquiária e capim-ruziziensis em crescimento inicial submetidos a diferentes doses dos herbicidas glyphosate e fluazifop-p-butil. Os tratamentos foram arranjados no esquema fatorial 2 × 6, com cinco repetições, sendo duas gramíneas: Brachiaria decumbens cv. Basilisk (capim-braquiária) e B. ruziziensis (capim-ruziziensis), e seis doses dos herbicidas: 0,00; 0,25; 0,50; 1,00; 1,5 e 2,00 vezes a dose comercial indicada pelos fabricantes. Aos 7, 15, 21 e 30 dias após aplicação dos herbicidas (DAA) foram avaliadas visualmente a intoxicação em capim-braquiária e capim-ruziziensis. O glyphosate causou intoxicação de 100% em plantas de capim-braquiária e capim-ruziziensis, mesmo na menor dose (90 g ha-1), podendo ser utilizado com eficiência para o controle dessas espécies no início de desenvolvimento. Foi observado que as plantas submetidas ao fluazifop-p-butil apresentaram rebrota apenas nas doses aplicadas até 100 g ha-1 para capim-braquiária e 50 g ha-1 para o capim-ruziziensis. Esses resultados confirmam a eficiência dessa molécula herbicida no controle dessas duas espécies a partir das doses de 200 g ha-1 e 100 g ha-1 para capim-braquiária e capim-ruziziensis, respectivamente.

English

Weeds are a limitation to cocoa production, and herbicide use in cocoa cultivation is known to lower the cost of weed management and support better plant growth. As a result, glyphosate was recommended for use in cocoa cultivation following a two-year field trial at the Cocoa Research Institute of Ghana. Recent informal debates among scientists and farmers, however, point towards a disagreement regarding the effect of glyphosate on young cocoa. Consequently, an experiment was conducted from 2011 to 2013 at the Cocoa Research Institute of Ghana to re-evaluate the suitability of glyphosate (Isopropylamine) for weed management and its effects on growth and yield of young cocoa. Five rates of glyphosate viz: (i) 1,920 g active ingredient a.i. ha-1 using polyjet nozzle; (ii) 1,920 g a.i. ha-1 using very low volume (vlv) nozzle; (iii) 960 g a.i. ha-1 vlv; (iv) 720 g a.i. ha-1 vlv; (v) 480 g a.i. ha-1 vlv; and (vi) manual weeding were tested in a randomized complete block design with four replicates in the field. Effects of treatments on weeds, cocoa growth and yield were recorded for three years. In a gauze house study, cowpea and maize were used to determine residual effects of glyphosate. Emergence, survival and dry matter accumulation by these plants were recorded. Results from the gauze house study showed that glyphosate did not exhibit residual soil activity at these rates. Glyphosate at 960 g ha-1 significantly increased yield of three year old cocoa compared to the other rates and manual weeding. The 1,920 g ha-1 rates significantly reduced the initial yield of 3 year old cocoa compared to the other glyphosate rates. Cost analysis showed that glyphosate at 960 g a.i. ha-1 was GhE¼ 136.00 (11%) and GhE¼ 1,784.00 (61%) cheaper than the 1,920 g a.i. ha-1 rates and manual weeding respectively. It was therefore concluded that glyphosate can be applied at 960 g a.i. ha-1 (equivalent to 2.0 l ha-1 in 120 l of water) for effective weed management without significant adverse effects on growth and yield of young cocoa. Key words: Weeds, glyphosate, cocoa, growth, bean yield and cost.

Sensitivity and Recovery of Grain Sorghum to Simulated Drift Rates of Glyphosate, Glufosinate, and Paraquat

A field experiment was conducted in 2017 and 2018 to evaluate the sensitivity and recovery of grain sorghum to the simulated drift of glufosinate, glyphosate, and paraquat at two application timings (V6 and flag leaf growth stage). Paraquat drift caused maximum injury to sorghum plants in both years, whereas the lowest injury was caused by glyphosate in 2017. Averaged over all herbicide treatments, injury to grain sorghum from the simulated herbicide drift was 5% greater when herbicides were applied at flag leaf stage, as compared to herbicide applications at the six-leaf stage in 2017. In 2018, injury from glyphosate drift was higher when applied at the six-leaf stage than at the flag leaf stage. Paraquat and glufosinate drift caused more injury when applied at flag leaf stage than at six-leaf stage at 14 days after application in 2018. About 21% to 29% of injury from the simulated drift of paraquat led to a 31% reduction in grain sorghum yield, as compared to a nontreated check in 2017. The simulated drift of glyphosate and glufosinate did not result in any significant yield reduction compared to the nontreated check in 2017, possibly due to the recovery of sorghum plants after herbicides’ drift application.

A deriva de glyphosate interfere no desenvolvimento e produtividade de arroz irrigado

Objetivo do trabalho foi avaliar os danos ao desenvolvimento e produtividade de cultivar de arroz irrigado de ciclo precoce (IRGA 430), ocasionados por deriva simulada de glyphosate. O experimento foi realizado a campo na Estação Experimental do Arroz – IRGA na safra 2016/17 e os tratamentos foram arranjados em esquema fatorial 3x2+1 cujo fator A constou da simulação de deriva de glyphosate nas doses de 135; 270 e 540g ha-1 e.a.; o fator B de duas doses de nitrogênio (N), sendo eles 90 e 121,5 kg ha-1 de N; mais testemunha sem aplicação de glyphosate e com aplicação de 90kg ha-1 de N. O aumento na dose de glyphosate resultou em maiores danos à cultura, como sintomas de fitotoxicidade, redução no número de panículas por área e no rendimento de grãos que foi de aproximadamente 60% na maior dose estudada. De acordo com o aumento da dose de glyphosate também ocorreu o prolongamento no número de dias entre a emergência da cultura até o estágio de desenvolvimento de antese (R4). A deriva simulada de glyphosate evidencia que o herbicida causa elevada fitotoxicidade a cultura, menor estatura as plantas e aumento do ciclo de desenvolvimento, interferindo negativamente na produtividade da cultura do arroz irrigado.

Glyphosate as Growth Regulator for Bahiagrass and Broadleaf Carpetgrass

ABSTRACT: The objective of this work was to evaluate the effect of glyphosate herbicide as growth regulator on two turfgrasses bahiagrass and broadleaf carpetgrass. The experiments were conducted in a greenhouse, using a completely randomized design, with 10 glyphosate rates (0, 5.625, 11.25, 22.5, 45, 90, 180, 360, 720, and 1.440 g a.e. ha-1) and four replicates. Bahiagrass and broadleaf carpetgrass plants were transplanted to 2 liter pots filled with a clayey soil. Phytotoxicity and green cover index (GCI) were evaluated through digital analysis; plant height at 7, 14, 21, and 28 days after application (DAA); and dry biomass of cuttings at 28 DAA. The glyphosate rates of 5.625 to 22.5 g a.e. ha-1 (for bahiagrass) and up to 90 g a.e. ha-1 (for broadleaf carpetgrass) reduced the plant growth, without affecting the plant visual quality phytotoxicity, GCI, and dry biomass in the evaluated periods. Contrastingly, rates equal to and above 45 g a.e. ha-1 (for bahiagrass) and equal to and above 180 g a.e. ha-1 (for broadleaf carpetgrass) caused phytotoxic effects in all evaluated periods and affected negatively plant height, GCI, and dry biomass, denoting the sensitivity of these grass species to these glyphosate rates. The digital image analysis allowed the verification and quantification of the effects of the herbicides on turfgrasses.

Quick Test for Detecting Glyphosate-Resistant Ryegrass Seeds

ABSTRACT: The commercialization of ryegrass seeds with the presence of resistant biotypes is a serious issue that increses the dispersion of resistance to new areas without this problem. Therefore, this study aimed to develop a quick test to detect susceptible and resistant seeds to the glyphosate herbicide in order to identify contaminated seed lots with glyphosate resistance. Three experiments were carried out, one in greenhouse and the other two in the seed laboratory. First, the resistance factor (RF) was determined by a dose-response curve experiment in biotypes suspected to be resistant and susceptible to glyphosate. Then, the germination test was conducted with the selected biotypes under increasing glyphosate rates (0, 3.5, 6.9, 13.9, 27.8, 55.5, 111, 222, 445, 890, and 1,780 mg a.e. L-1), as the second experiment. The third experiment was made to verify the methodological efficiency of the germination test with glyphosate to identify different contamination ratios (0, 4, 12, 36, and 100%) of the resistant biotype in the seed lot. The different levels of susceptibility of the biotypes to glyphosate were confirmed by the RF of 154.7 based on C50. Germination of the susceptible biotype was inhibited more than 99% by the rate of 127 mg a.e. L-1, while the resistant biotype was a little affected. The germination test with 127 mg a.e. L-1 of glyphosate showed contaminations of 2, 5, 19, 39, and 86% in lots with 0, 4, 12, 36, and 100% of contaminated seeds, respectively. This methodology can detect glyphosate susceptible and resistant seeds and identify contaminated seed lots with resistant glyphosate biotypes.

Effect of Low Doses of Dicamba Alone and in Combination with Glyphosate on Parent Soybean and Offspring

AbstractIt is well established that soybean that does not contain the dicamba-resistant (DR) trait is highly sensitive to off-target exposure to dicamba. However, there is limited information on the effect of low doses of dicamba plus glyphosate mixtures on dicamba-sensitive soybean—a mixture likely to be used on a vast acreage of dicamba/glyphosate-resistant soybean. The objective of this research was to examine leaf and pod malformation, along with height and yield effects, when dicamba, glyphosate, or a mixture of the two was applied to soybean sensitive to both dicamba and glyphosate at sublethal doses. Field applications were made at three growth stages (R1, R3, and R5) at multiple locations. Two glyphosate rates (1/64 and 1/256 of the labeled rate of 870 g ae ha−1) and two dicamba rates (1/64 and 1/256 of the labeled rate of 560 g ae ha−1) were used. Adding glyphosate to dicamba increased leaf malformation by 6% more than dicamba alone when applied at the R1 soybean growth stage. After R3 applications, pod malformation was 10% greater in treatments containing dicamba and glyphosate than dicamba alone. Applications at R5 showed minimal leaf and pod malformation. Seed from field trials was planted in the greenhouse to evaluate the offspring. The number of offspring plants showing dicamba-like symptomology was not increased with the addition of glyphosate to dicamba. Overall, injury to offspring was similar in dicamba alone and dicamba plus glyphosate treatments; however, the number of plants injured increased when parent plants were exposed to sublethal doses of dicamba at R3 and R5 compared with R1 growth-stage exposure. Vigor was reduced in dicamba-containing treatments, but not glyphosate-alone treatments. Glyphosate addition to dicamba had no effect on vigor of soybean offspring. Although there is increased injury to parent plants when glyphosate is added to dicamba, this research demonstrates that glyphosate does not contribute to the negative effects of dicamba on soybean offspring.

Biogenic transport of glyphosate in the presence of LDPE microplastics: A mesocosm experiment

The accumulation of plastic debris and herbicide residues has become a huge challenge and poses many potential risks to environmental health and soil quality. In the present study, we investigated the transport of glyphosate and its main metabolite, aminomethylphosphonic acid (AMPA) via earthworms in the presence of different concentrations of light density polyethylene microplastics in the litter layer during a 14-day mesocosm experiment. The results showed earthworm gallery weight was negatively affected by the combination of glyphosate and microplastics. Glyphosate and AMPA concentrated in the first centimetre of the top soil layer and the downward transport of glyphosate and AMPA was only detected in the earthworm burrows, ranging from 0.04 to 4.25 μg g−1 for glyphosate and from 0.01 (less than limit of detection) to 0.76 μg g−1 for AMPA. The transport rate of glyphosate (including AMPA) from the litter layer into earthworm burrows ranged from 6.6 ± 4.6% to 18.3 ± 2.4%, depending on synergetic effects of microplastics and glyphosate application. The findings imply that earthworm activities strongly influence pollutant movement into the soil, which potentially affects soil ecosystems. Further studies focused on the fate of pollutants in the microenvironment of earthworm burrows are needed.

Agronomic Performance of Intacta RR2 Soybean Submitted to Doses of Glyphosate

ABSTRACT: This study aimed at evaluating the agronomic performance of Intacta RR2 soybean submitted to the application of glyphosate doses in post-emergence. For this, two experiments were conducted in two locations (Palotina and Marechal Cândido Rondon - Paraná state). Applications were carried out with increasing rates of glyphosate (0, 720, 1,440, 2,160, 2,880, 3,600, 4,320 g a.e. ha-1), constituting seven treatments with an experimental design in randomized blocks with four replications, totaling 28 plots for each location. The cultivar used for both experiments was Monsoy 6210 Intacta RR2 IPROTM. The analyzed variables were phytotoxicity at 3, 7, 14, 21 and 28 DAA, A-B and total chlorophyll index, final height, number of pods, 100-seed weight, and yield. A regression analysis was used. There was a positive behavior of the linear regression for phytotoxicity and a negative one for final height, number of pods, A-B and total chlorophyll index, and yield in the city of Palotina. In Marechal Cândido Rondon, there were adjustments of the positive linear regressions for phytotoxicity, and negative linear regressions for chlorophyll index, final height, number of pods and yield. Results show that the glyphosate increase from the dose 1,440 g a.e. ha-1 in the studied cultivar damaged the agronomic performance of the culture.

Exploring the half-life of glyphosate in human urine samples

The International Agency for Research on Cancer (IARC) has recently classified glyphosate as a Group 2A 'probably carcinogenic to humans'. Due to this carcinogenic classification and resulting international debate, there is an increased demand for studies evaluating human health effects from glyphosate exposures. There is currently limited information on human exposures to glyphosate and a paucity of data regarding glyphosate's biological half-life in humans. This study aims to estimate the human half-life of glyphosate from human urine samples collected from amenity horticulture workers using glyphosate based pesticide products. Full void urine spot samples were collected over a period of approximately 24 h for eight work tasks involving seven workers. The elimination time and estimation of the half-life of glyphosate using three different measurement metrics: the unadjusted glyphosate concentrations, creatinine corrected concentrations and by using Urinary Excretion Rates (UER) (μg L-1, μmol/mol creatinine and UER μg L-1) was calculated by summary and linear interpolation using regression analysis. This study estimates the human biological half-life of glyphosate as approximately 5 ½, 10 and 7 ¼ hours for unadjusted samples, creatinine corrected concentrations and by using UER (μg L-1, μmol/mol creatinine, UER μg L-1), respectively. The approximated glyphosate half-life calculations seem to have less variability when using the UER compared to the other measuring metrics. This study provides new information on the elimination rate of glyphosate and an approximate biological half-life range for humans. This information can help optimise the design of sampling strategies, as well as assisting in the interpretation of results for human biomonitoring studies involving this active ingredient. The data could also contribute to the development or refinement of Physiologically Based PharmacoKinetic (PBPK) models for glyphosate.

The First Cases of Evolving Glyphosate Resistance in UK Poverty Brome (Bromus sterilis) Populations

AbstractPoverty brome (Bromus sterilisL.) [sterile or barren brome, syn.Anisantha sterilis(L.) Nevski] is a problematic UK arable weed. There are currently no confirmed cases of glyphosate resistance in any weed species in the United Kingdom or inB. sterilisworldwide. However, there are reports of poor control by glyphosate in this species. Here, we report experiments to confirm the suspected on-farm resistance ofB. sterilispopulations to glyphosate. Glyphosate screening and dose–response experiments established that glyphosate sensitivity of three UKB. sterilispopulations exhibiting poor field control is outside the normal range of sensitivity of 30 sensitive populations and adjacent unexposed populations. Control of sensitive populations ranged from 49% to 82% and for suspected resistant populations from 21% to 30%. Dose–response ED50values of sensitive populations ranged between 241 and 313 g ai ha−1; corresponding values of suspected resistant populations ranged between 420 and 810 g ha−1, and resistance indices ranged from 1.55 to 4.5. Suspected resistant populations were incompletely controlled at the recommended field rate of glyphosate (540 g ha−1), while adjacent unexposed populations were completely controlled. We conclude that some UK populations ofB. sterilishave reduced glyphosate sensitivity and are in the process of evolving resistance. This is the first reported case of reduced glyphosate sensitivity in any UK weed species and inB. sterilisworldwide. This, coupled with increasing glyphosate use, highlights the need for increased vigilance and monitoring for glyphosate resistance in the United Kingdom.

Insecticide Seed Treatments Partially Safen Rice to Low Rates of Glyphosate and Imazethapyr

AbstractEach year there are multiple reports of drift occurrences, and the majority of drift complaints in rice are from imazethapyr or glyphosate. In 2014 and 2015, multiple field experiments were conducted near Stuttgart, AR, and near Lonoke, AR, to evaluate whether insecticide seed treatments would reduce injury from glyphosate or imazethapyr drift or decrease the recovery time following exposure to a low rate of these herbicides. Study I was referred to as the “seed treatment study,” and Study II was the “drift timing study.” In the seed treatment study the conventional rice cultivar ‘Roy J’ was planted, and herbicide treatments included imazethapyr at 10.5 g ai ha–1, glyphosate at 126 g ae ha–1, or no herbicide. Each plot had either a seed treatment of thiamethoxam, clothianidin, chlorantraniliprole, or no insecticide seed treatment. The herbicides were applied at the two- to three-leaf growth stage. Crop injury was assessed 1, 3, and 5 wk after application. Averaged over site-years, thiamethoxam-treated rice had less injury than rice with no insecticide seed treatment at each rating, along with an increased yield. Clothianidin-treated rice had an increased yield over no insecticide seed treatment, but the reduction in injury for both herbicides was less pronounced than in the thiamethoxam-treated plots. Overall, chlorantraniliprole was generally the least effective of the three insecticides in reducing injury from either herbicide and in protecting rice yield potential. A second experiment conducted at Stuttgart, AR, was meant to determine whether damage to rice from glyphosate and imazethapyr was influenced by the timing (15, 30, and 45 d after planting) of exposure to herbicides for thiamethoxam-treated and nontreated rice. There was an overall reduction in injury with the use of thiamethoxam, but the reduction in injury was not dependent on the timing of the drift event. Reduction in damage from physical drift of glyphosate and imazethapyr as well as increased yields over the absence of an insecticide seed treatment appear to be an added benefit.