The purpose of this study is to clarify the differential effects of the application rate of slow-release nitrogen fertilizer (SRFN) on the nitrogen (N) accumulation dynamics, nutrient organ N distribution and transportation, yield, and N utilization efficiency of maize harvested using grain-type machines. This has significant implications for the scientific application of SRFN, as well as for reducing its application rate and improving its efficiency, in the agro-pastoral transitional zone of northern China. In a long-term positioning experiment that began in 2018, five treatments consisting of different SRFN application rates were set up, namely, N120 (120 kg ha−1), N180 (180 kg ha−1), N240 (240 kg ha−1), N300 (300 kg ha−1), and N360 (360 kg ha−1), with no fertilization during the growth period used as control (CK) treatment. To explore the characteristics of nitrogen accumulation dynamics in maize populations and the main factors affecting maize yield formation under the different SRFN application rate treatments, this study adopted a combination of quantitative analyses and model fitting, including logistic models, principal component analysis, and structural equation modeling. The research results show that SRFN application increased the aboveground N accumulation of the maize population, and the fitting effect of the logistic models was significant. The maximum rate of N accumulation in both years showed a trend of first increasing and then decreasing with the increase in the SRFN application rate. Compared with CK, SRFN application reduced the proportion of N distribution in the nutrient organs during the R6 stage, and it increased the N transport from the nutrient organs to the grains after the VT-R1 stage. With the increase in the SRFN application rate, both the economic yield and biological yield showed a single peak curve change and were maximized in the N240 treatment. The economic yield reached 15,342.07 kg ha−1 in 2020 and 16,323.51 kg ha−1 in 2021, increasing by 36.2% and 61.7% compared with CK, respectively. The apparent N fertilizer recovery rate, N uptake efficiency, N agronomic efficiency, and N fertilizer partial productivity all gradually decreased with the increase in the SRFN application rate. In maize populations, an appropriate SRFN application rate can adjust the characteristic parameters during the aboveground N accumulation rapid growth period, increase the N accumulation amount in aboveground parts, promote the transport of N from nutrient organs to grains, and improve yield. An application of 180–240 kg ha−1 SRFN is recommended for maize cultivation in the agro-pastoral transitional zone of northern China, as it is beneficial for stabilizing and increasing maize yield, as well as reducing the rate and improving the efficiency of N fertilizer.
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