The present study deals with experimental measurements of CO2 generation rates, due to the human occupation of a full-scale experimental mock-up simulating the astronaut crew quarters aboard the International Space Station. The estimation of CO2 generation rates follows different methods as described in the literature. A single test subject in four different testing cases is considered, one at rest representing the baseline case and the other three cases at varying levels of physical activity or at rest but with a fixed breathing frequency requested from the human subject. The study results indicate that imposing a fixed breathing rate even while at rest increases the generation rate unpredictably. Following literature metabolic rate estimations, the latter two cases are equivalent to the subject being engaged in light or medium physical activities. The results are used to form recommendations for studies measuring human CO2 generation rates.