Abstract A simple, rapid and sensitive fluorimetric method is described for the determination of metyrosine in bulk, in dosage forms and in biological fluids. The method is based on measuring the fluorescence produced in isopropanol. The fluorescence is measured at 610 nm after excitation at 282 nm. The fluorescence intensity is a linear function of metyrosine concentration over the range of 0.2–3.2 μg ml−1 with a detection limit of 0.08 μg ml−1. The advantages of the proposed method over other methods were discussed.