Abstract
The stopped-flow mixing technique was used to investigate the application of kinetic methodology to complex formation reactions involving energy transfer processes. By using initial rate measurements a very rapid fluorimetric method was developed for the determination of diphacinone by formation of the ternary complex Eu(III)-di-phacinone-ammonia in the presence of Triton X-100. Kinetic data can be obtained only 2–3 s after the reacts are mixed, which allows ready application of the method to routine analyses. The linear response range of the proposed method is 0.5–5 μg ml −1 diphacinone and the mid-range relative standard deviation is ca. 2%. The mean recovery obtained in the analysis of serum samples was 98.3%.
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