Radish Research Articles

Comparative growth analysis of Raphanus sativus L. (Radish): effects of vermiwash and vermicompost applications on plant development

Long-term application of chemical fertilizers has negative impacts on soil health, crop productivity, and ecosystems. Hence, organic substrates have been proposed as alternatives for enhancing soil health and plant growth parameters. Among them, vermicompost and vermiwash are widely used in the sustainable agriculture sector to enhance plant growth, yield, and soil fertility. Hence, in this study, we aimed to investigate the effects of vermiwash and vermicompost on the growth of Raphanus sativus L. Pot culture experiments were conducted in four groups which included: group A) control soil (10 kg pot−1), group B) soil (8.5 kg pot−1) + vermicompost (1.5 kg pot−1), group C) soil (10 kg pot−1) + vermiwash (1.5 L pot−1), and group D) soil (8.5 kg pot−1) + vermicompost (1.5 kg pot−1) + vermiwash (1.5 L pot−1), respectively. The results revealed that the plants treated with vermiwash and vermicompost (Group D) had significantly enhanced the growth of the plants with higher germination percentage (100%), number of leaves (32.08%), length of leaves (56.35%), width of leaves (28.05), area of leaves (100.22%), shoot length of plant (78.02%), chlorophyll concentration (42.72%), leaf relative water content (13.30%), fresh weight (65.61%), dry weight of leaves (57.59), and tuber yield (74.96%) when compared to plants under groups A, B, and C. Furthermore, the application of the vermiwash and vermicompost combination improved the soil's physicochemical properties, increased glycine betaine concentrations, and decreased proline accumulation. Hence, this study suggests that vermicompost and vermiwash may be a potential source of plant nutrients for sustainable crop production.

Influence of Growth Regulators on Growth, Seed Yield and Qualty of Radish (Raphanus sativus L.)

The present investigation was carried out during 2021 at the experimental farm of Faculty of Agricultural Sciences, DAV University, Jalandhar. Radish is an important crop of Punjab region. Use of Plant Growth Regulators is one of the most recent trends in the field of agriculture science. Plant growth regulators are signal molecules, which occurs in plants naturally in very low concentration. In an attempt to find out the influence of growth regulators on growth, seed yield and quality of radish (Raphanus sativus L.) the experiment was planned and executed. It consisted of nine treatments viz., T1 (Control), T2 (GA3 @ 5 ppm), T3 (GA3 @ 10 ppm), T4 (GA3 @ 15 ppm), T5 (GA3 @ 20 ppm), T6 (NAA @ 5 ppm), T7 (NAA @ 10 ppm), T8 (NAA @ 15 ppm), T9 (NAA @ 20 ppm) and was laid in Randomized block design (RBD) with three replications. Punjab Safed Mooli variety was used for the experiment. Observations were recorded on number of leaves per plant, leaf area per plant (cm2), root length (cm), root diameter (cm), root weight (g), fresh weight of plant (g), dry weight of plant (g), plant height (cm), 100 seed weight (g), seed yield plant-1 (g), seed yield plot-1 (g), seed yield ha-1 (q ha-1), TSS (°Brix), ascorbic acid (mg 100 g-1), chlorophyll content (mg 100 g-1) and germination percentage (%). Analysis of variance (ANOVA) revealed significantly differences among treatments for all the characters under study. It was concluded that the application of GA3 @ 20 ppm showed superior performance in all growth, seed yield and quality parameters of radish.

RsOBP2a, a member of OBF BINDING PROTEIN transcription factors, inhibits two chlorophyll degradation genes in green radish

The green radish (Raphanus sativus L.) contains abundant chlorophyll (Chl). DOF-type transcription factor OBF BINDING PROTEIN (OBP) plays crucial functions in plant growth, development, maturation and responses to various abiotic stresses. However, the metabolism by which OBP transcription factors regulate light-induced Chl metabolism in green radish is not well understood. In this study, six OBP genes were identified from the radish genome, distributed unevenly across five chromosomes. Among these genes, RsOBP2a showed significantly higher expression in the green flesh compared to the white flesh of green radish. Analysis of promoter elements suggested that RsOBPs might be involved in stress responses, particularly in light-related processes. Overexpression of RsOBP2a led to increase Chl levels in cotyledons and adventitious roots of radish, while silencing RsOBP2a expression through TYMV-induced gene silencing accelerated leaf senescence. Further investigations revealed that RsOBP2a was localized in the nucleus and served as a transcriptional repressor. RsOBP2a was induced by light and directly suppressed the expression of STAYGREEN (SGR) and RED CHLOROPHYLL CATABOLITE REDUCTASE (RCCR), thereby delaying senescence in radish. Overall, a novel regulatory model involving RsOBP2a, RsSGR, and RsRCCR was proposed to govern Chl metabolism in response to light, offering insights for the enhancement of green radish germplasm.

Alien species of nectar-bearing plants of Ukraine’s flora

Alien species pose a significant threat to global biodiversity, competing with local species, gradually displacing them, and often leading to their demise. Alien plants, in particular, pose a direct threat to native plant diversity as well as the management of ecosystems, agriculture, and forestry. The main reason for the appearance of alien species in ecosystems where they are not native is human activity. Most alien plant species have become so due to introduction for cultivation purposes, thanks to their medicinal, honey-producing, and other beneficial properties for humans. Therefore, knowledge of their biology, potential for expansion, as well as possible monitoring and control methods, is extremely important. After all, obtaining the maximum benefit for meeting public needs without harming the environment is an urgent task today. Among alien species, invasive species are the most dangerous for the loss of biodiversity. However, currently in Ukraine, there are no officially approved lists of alien and invasive species, their inventory has not been conducted, monitoring and risk assessment systems are absent, and there are no adequate and coordinated measures to counteract biotic invasions. Therefore, the purpose of our work was to conduct an analysis of the flora of Ukraine, to identify alien, including invasive plants, are honey and/or pollen-producing, and compile their list. As a result of the analysis, 63 alien, including 16 invasive species of vascular plants with honey and pollenproducing properties were identified, belonging to 28 families and 56 genera. Particularly valuable invasive honey plants include: Helianthus annuus L., Robinia pseudoacacia L., Asclepias syriaca L., Paulownia tomentosa (Thunb.) Siebold & Zucc., Solidago canadensis L., which can provide significant industrial honey production. Considering that such invasive plants (which are also honey-producing) as Cucurbita pepo L., Raphanus sativus L., Helianthus annuus L., Juglans nigra L., Prunus armeniaca L. and Vitis vinifera L. are agricultural crops, their spread is usually limited to agricultural lands. Therefore, their invasions into natural ecosystems are minimal, and the benefits are undeniable. However, when cultivating alien plants, it is always worth considering the benefit and economic gain against their real or potential threat to natural ecosystems.

Development of Raphanus sativus L. is Stimulated by Irrigation with Magnetically Treated Water

Radish irrigation promotes the best development of the crop; however, at the same time, it has to be employed efficiently. The present work aims to determine the effect of magnetically treated water on the agronomic performance of Raphanus sativus L. The experimental design used was completely randomized with two treatments consisting of two types of irrigation [magnetically treated water (MTW) and without (WWMT)], with 3 replications of 20 plants each. Seeds of Raphanus sativus L. cultivar (Alegro radish) were used. Magnetic treatment was performed using a magnetic device with a nonuniform or heterogeneous static magnetic field between 100 and 200 mT. During crop development, the number of leaves (NL) and plant height (PH) were evaluated, and at harvest, the average fruit weight (FW) and radish diameter (RD) were evaluated. ANOVA showed that there were significant differences when comparing irrigation treatments in favor of MTW only for the variables NL at 21 days after sowing (DAS) and RD at the end of the crop cycle (35 DAS); however, MTW stimulated all the variables [plant height (9%), number of leaves (5%), fruit diameter (25%), and average fruit weight (4%)]. It was concluded that, under the conditions of this study, irrigation with magnetically treated water leads to changes in radish plant morphology and can be recommended as a treatment that stimulates crop development. Keywords: Radish. Drip. Sustainable Production. Agronomic Management. Resumo A irrigação do rabanete promove o melhor desenvolvimento da cultura, entretanto, ao mesmo tempo tem que ser empregada de modo eficiente. O presente trabalho tem como objetivo determinar o efeito da água tratada magneticamente no desempenho agronômico do Raphanus sativus L. O delineamento experimental utilizado foi completamente aleatorizado com dois tratamentos que consistiram em dois tipos de irrigação [água com tratamento magnético (ACTM) e sem (ASTM)], com três repetições de 20 plantas cada. Foram empregadas sementes da cultivar de Raphanus sativus L. (rabanete Alegro). O tratamento magnético foi realizado através de um dispositivo magnético com um campo magnético estático não uniforme ou heterogêneo entre 100 e 200 mT. Durante o desenvolvimento da cultura foi avaliada número de folhas (NF) e altura da planta (AP), e na colheita foram avaliados o peso médio dos frutos (PF) e o diâmetro dos frutos (DR). A ANOVA mostrou que existe diferencias significativas quando comparados os tratamentos de irrigação a favor da ACTM apenas para as variáveis NF aos 21 dias após semeadura (DAS) e no DR ao final do ciclo da cultura (35 DAS), entretanto, a ACTM estimulou todas as variáveis [altura da planta (9%), número de folhas (5%), diâmetro dos frutos (25%), e peso médio dos frutos (4%)]. Concluiu-se que, nas condições deste estudo, a irrigação com água tratada magneticamente leva a alterações na morfologia das plantas de rabanete e pode ser recomendado como um tratamento que estimula o desenvolvimento da cultura. Palavras-chave: Rabanete. Gotejo. Produção Sustentável. Manejo Agronômico.

Understanding the role of magnetic (Fe3O4) nanoparticle to mitigate cadmium stress in radish (Raphanus sativus L.)

Heavy metals stress particularly cadmium contamination is hotspot among researchers and considered highly destructive for both plants and human health. Iron is examined as most crucial element for plant development, but it is available in inadequate amount because they are present in insoluble Fe3+ form in soil. Fe3O4 have been recently found as growth promoting factor in plants. To understand, a sand pot experiment was conducted in completely randomized design (control, cadmium, 20 mg/L Fe3O4 nanoparticles,40 mg/L Fe3O4 nanoparticles, 20 mg/L Fe3O4 nanoparticles + cadmium, 40 mg/L Fe3O4 nanoparticles + cadmium) to study the mitigating role of Fe3O4 nanoparticles on cadmium stress in three Raphanus sativus cultivars namely i.e., MOL SANO, MOL HOL PARI, MOL DAQ WAL. The plant growth, physiological and biochemical parameters i.e.,shoot length, shoot fresh weight, shoot dry weight, root length, root fresh and dry weight, MDA content, soluble protein contents, APX, CAT, POD activities and ion concentrations, membrane permeability, chlorophyll a, chlorophyll b and anthocyanin content, respectively were studied. The results displayed that cadmium stress remarkably reduces all growth, physiological and biochemical parameters for allcultivars under investigation. However, Fe3O4 nanoparticles mitigated the adverse effect of cadmium by improving growth, biochemical and physiological attributes in all radish cultivars. While, 20 mg/L Fe3O4 nanoparticles have been proved to be more useful against cadmium stress. The outcome of present investigation displayed that Fe3O4 nanoparticles can be utilized for mitigating heavy metal stress.

Nitrogen form mediates sink strength and resource allocation of a C3 root crop under elevated CO2

Plant physiological processes alter with elevated carbon dioxide concentrations in the atmosphere (eCO2), which affects the growth and yield potential of crops. Among plants with C3 carbon fixation, root crops show highest yield response under eCO2 which is suggested to be linked to their large carbon (C) sink strength. The high C gain under eCO2 can be limited by processes that constrain sink capacity, such as nitrogen (N) supply. Different N sources may interact with eCO2 and thus have variable impacts on carboxylation activity, N uptake efficiency and plant development. This study aims at contributing to a better understanding of sink-driven assimilation, re-allocation and finally growth processes under eCO2 as a function of N-form. Radish (Raphanus sativus L. var. sativus), a C3 tuber plant with strong sink strength, was used. The plants were grown in pots in climate chambers at 400 ppm (aCO2) and 1000 ppm CO2 (eCO2) with either pure nitrate or ammonium-dominated nutrition. A split plot design was applied. Plants were harvested after four weeks and physiological, morphological and chemical parameters in leaves and tubers were assessed. The N-form had no effect on N acquisition, but affected C and N partitioning into plant organs differently under eCO2. N assimilation processes of nitrate-fed plants were focussed on leaves being both source and sink, and those of ammonium-fed plants on tubers, the strongly pronounced sink. Acclimation of CO2 fixation due to eCO2 did not occur for both N forms, probably due to altered sink strength and low N content in leaves. The N-form influences the sink-driven C and N balance and thus enables unrestricted carbon gain as well as the variation of organ development under future eCO2 conditions. These processes can be utilized in cultivation and breeding.

Assessment of black rot (Xanthomonas campestris) of cabbage (Brassica oleracea) in East Khasi Hills, Meghalaya

Cabbage (Brassica oleracea L. var. capitata) holds significant economic importance in Meghalaya, thriving in its high altitude and cool climate. East Khasi Hills (EKH) district, where cabbage cultivation remains viable throughout the year, contributes 70% of the state's total cabbage production. A severe outbreak of cabbage black rot, caused by Xanthomonas campestris p.v. campestris, has raised concerns in this region. To comprehensively understand the outbreak and assess its economic impact, an extensive disease survey was conducted across 6 districts of Meghalaya, with a particular focus in the EKH during 2021–22 and 2022–23. The survey was on different cruciferous vegetables such as cabbage, broccoli (Brassica oleracea L. var. italica), cauliflower (Brassica oleracea L. var. botrytis), radish (Raphanus sativus L.), mustard (Brassica juncea L.), and knol khol (Brassica oleracea L. var. gongylodes). It was found that on an average, Meghalaya experiences a disease incidence (DI) of a 52.7% and per cent disease index (PDI) of 30.9% for black rot in cabbage. However, in the EKH, highest disease incidence (69.98%) and PDI (43.38%) of black rot was recorded in cabbage, that surpassed knol khol and cauliflower. Highest DI and severity were observed during monsoon season. Further analysis revealed significant correlations (r) between black rot incidence and severity in cabbage with temperature (0.91), humidity (0.87) and rainfall (0.88). Additionally, multiple regression predictive model performed using weather parameters and DI led to the prediction of PDI with accuracy of 95% in EKH.

Black radish root extract alleviates sodium valproate-induced liver damage via inhibiting mitochondrial membrane potential collapse and oxidative stress in mice

Objective: To explore the effect of black radish (Raphanus sativus L. var niger) root extract on liver enzymes, oxidative stress, and histopathological alterations in mice with sodium valproate-induced hepatotoxicity. Methods: Thirty-two mice were divided into four groups: the control group received drinking water by gavage, the second group was administered with 100 mg/kg of sodium valproate, the third group received 300 mg/kg of black radish root extract, and the fourth group was given both sodium valproate (100 mg/kg) and black radish root extract (300 mg/kg). After 28 days, the mice were euthanized, and serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), along with liver malondialdehyde (MDA), reactive oxygen species (ROS), mitochondrial parameters, tumor necrosis factor-alpha (TNF-α) gene expression, and histopathological changes were assessed. Results: Sodium valproate caused hepatic damage in mice, characterized by elevated serum levels of liver enzymes, increased MDA and ROS levels and TNF-α gene expression, as well as histopathological alterations. The black radish root extract significantly alleviated sodium valproate-caused hepatic injury by decreasing the serum levels of ALT and AST, MDA, ROS, TNF-α gene expression, as well as mitochondrial impairment, but did not have a significant effect on sodium valproate-induced histopathological changes. Conclusions: The black radish root extract demonstrates protective effects against sodium valproate-induced liver injury, possibly through mitigating oxidative stress, mitochondrial impairment, and inflammatory mediator expression.

Therapeutic Potential of Brassica oleracea and Raphanus sativus Aqueous Extracts on a Prediabetic Rat Model.

Type 2 diabetes (T2D) is a serious health problem, and its prevalence is expected to increase worldwide in the years ahead. Cruciferous vegetables such as Brassica oleracea var. capitata L. (green cabbage) and Raphanus sativus L. (radish) have therapeutic properties that can be used to support the treatment of T2D. This study evaluated the effect of B. oleracea (BAE) and R. sativus (RAE) aqueous extracts on zoometric parameters, glycemic profiles, and pancreas and liver in prediabetic rats induced by a high-sucrose diet (HSD). BAE and RAE were administered to male HSD-induced Wistar rats (n = 35) at 5 and 10 mg/kg doses for 5 weeks. Zoometric and biochemical changes were measured, and then the pancreas and liver histological preparations were analyzed to observe the protective effect. BAE decreased feed intake and weight gain. Both extracts decreased fasting glucose and insulin levels compared with control (not treated), although not significantly (P > .05). The extracts significantly (P < .05) reduced homeostatic model assessment for insulin resistance, homeostasis model assessment of β-cell function, and glucose intolerance, similar to metformin control. In addition, minor damage occurred in the pancreas and liver. The results indicated that BAE and RAE decreased weight gain, improved glucose regulation, and protected the pancreas and liver in HSD rats. Therefore, they have multiple therapeutical properties and may be helpful in the prevention of T2D.

Effect of Raphanus Sativus (Radish) Leaf Extract and High Doses of Atorvastatin on Lipid Profile

Effect of Raphanus Sativus (Radish) Leaf Extract and High Doses of Atorvastatin on Lipid Profile

Biofumigation Treatment Using Tagetes patula, Sinapis alba and Raphanus sativus Changes the Biological Properties of Replanted Soil in a Fruit Tree Nursery

Apple replant disease (ARD) may cause significant losses both in commercial orchards and in fruit tree nurseries. The negative effects of ARD may be limited by using biofumigation. The aim of the study was to assess the influence of this treatment on the biological properties of replanted soil in a tree nursery. In two-year experiment, apple trees of the ‘Golden Delicious’ cultivar were used. The trees were planted into soil from two sites. The soil from one site had not been used in a nursery before (crop rotation soil). The other soil had been used for the production of apple trees (replanted soil). Three species of plants were used in the replanted soil as a forecrop: French marigold (Tagetes patula), white mustard (Sinapis alba), and oilseed radish (Raphanus sativus var. oleifera). The following parameters were assessed in the experiment: the enzyme and respiratory activity of the soil, the total count of bacteria, fungi, oomycetes and actinobacteria in the soil, as well as the count and species composition of soil nematodes. The vegetative growth parameters of the apple trees were also assessed. The biological properties of the replanted soil were worse than those of the crop rotation soil. In the replanted soil, the organic matter content, enzyme and respiratory activity as well as the count of soil microorganisms were lower. The biofumigants, used as a forecrop on the replanted soil, significantly increased its enzyme activity and respiratory activity. Dehydrogenase activity increased more than twofold. Growth parameters of the trees were significantly improved. The height of the trees increased by more than 50%, and the leaf area, weight and total length of side shoots were higher as well. The density of nematodes in the replanted soil after biofumigation was significantly reduced, with a larger reduction in the marigold fumigated soil. Eight of the eleven nematode species were completely reduced in the first year after biofumigation treatment.

Influence of cover crops on soil aggregate stability, size distribution and related factors in a no-till field

Soil aggregation plays an essential role in maintaining soil structure. A three-year field experiment was undertaken to explore the effects of cover crops on soil aggregate stability indices (mean weight diameter, water-stable aggregates greater than 0.25 mm, and soil erodibility factor), size distribution and their correlations with nine soil physical and chemical properties (soil bulk density, pH, organic carbon, total nitrogen, C/N, calcium, phosphorus, potassium, and cation exchange capacity) at 0–5 and 5–10 cm depths in a Marietta silt loam soil in a no-till field, Northeastern Mississippi. The cover crop treatments included elbon rye (Secale cereale L.), daikon radish (Raphanus sativus ssp. acanthiformis), Austrian winter field peas (Lathyrus hirsutus), and their mixture. The samples were wet-sieved to five size classes of aggregates (> 2, 2–1, 1–0.5, 0.5–0.25, and < 0.25 mm). Results showed that the < 0.25 mm aggregates dominated (50.95–78.02 %) soil aggregate size distribution at 0–5 and 5–10 cm depths. The rye plot soil displayed the greatest values for mean weight diameter (0.58 mm) in the two soil depths. Soil water-stable aggregates greater than 0.25 mm value under peas was significantly higher by 68.61 % compared to the no cover crop treatment at 0–5 cm depth, while soil erodibility factor did not change significantly. Mean weight diameter, water-stable aggregates greater than 0.25 mm, soil erodibility factor, 1–0.5 mm aggregates, and < 0.25 mm aggregates were significantly correlated with soil organic carbon, pH, bulk density, phosphorus, and potassium. The dominant factors driving changes in three aggregate stability indices and size fractions (excluding 2–1 mm aggregates) were bulk density and pH. Furthermore, high pH restricted soil aggregate stability and size distribution. This study demonstrated the positive impact of cover crops, specifically peas and rye, on soil aggregate stability and size distribution. This study will provide valuable information for future research on soil stabilization in crop production management systems.

The Effect of Recycled Spent Coffee Grounds Fertilizer, Vermicompost, and Chemical Fertilizers on the Growth and Soil Quality of Red Radish (Raphanus sativus) in the United Arab Emirates: A Sustainability Perspective.

The overuse of chemical fertilizers degrades the soil ecosystem and restricts the natural development of plants. Various byproducts are produced throughout the production and consumption of coffee within the coffee industry, and they are significant in terms of environmental waste. Spent coffee grounds (SCGs) contains various bioactive compounds that have demonstrated potential applications in various fields. These compounds can enhance soil quality by improving its physicochemical properties and biological fertility, ultimately leading to improved plant growth and reducing food waste and contamination at the same time. This current study examined the impact of chemical fertilizer, vermicompost, SCGs with percentage fertilizer (SCGPF), and SCGs on the top dressing fertilizer (SCGTDF) on red radish (Raphanus sativus) growth and soil quality. This greenhouse experiment tested various concentrations of SCGPF (5%, 10%, 25%, and 50%) and different doses of SCGTDF (0.5 g, 1 g, and 2.5 g). The results showed that the 0.5 g SCGTDF treatment yielded the highest mean plant length (18.47 cm) and fresh weight (27.54 g), while the vermicompost at a 50% concentration produced the highest mean leaf surface area (58.32 cm2). These findings suggest the potential of SCGs as a sustainable fertilizer alternative, contributing to improved plant growth and soil quality, thus supporting sustainable agricultural practices and a circular economy.

A comprehensive model for estimating the shelf life of young radish (Raphanus sativus L.) kimchi packaged in partially breathable film pouches

Continuous fermentation by lactic acid bacteria (LAB) causes packaging bursting, presenting a challenge in the commercialization of kimchi by reducing its shelf life. This study explored the use of partially breathable film pouches (PBFP) as a packaging solution for young radish kimchi (YRK). Furthermore, the shelf life of YRK was estimated through multivariate accelerated shelf life testing (MASLT). Packaged YRK samples were stored for 60 d at different temperatures (0, 5, 10, and 15 °C). The gas permeability of the PBFP and LAB metabolism controlled the volume and headspace gas composition of the packaged YRK samples during storage. The PBFP did not negatively impact YRK quality. Principal component (PC) analysis successfully reduced the physicochemical and microbial characteristics of YRK to a single variable. The exponential plateau model better described the variation in the time-related PC score than the zero- and first-order models. The P value within 15% of the comprehensive model, incorporating exponential and Arrhenius models, indicated its adequacy for predicting the shelf life of YRK. This study offers both insights into packaging technology and a predictive model, contributing to the improvement of the distribution management system for fermented vegetables.

Glycine Betaine Induces Tolerance to Oxidative Stress in Cherry Radishes under High-Temperature Conditions

Cool-season plant growth and development are impacted by high temperatures. As a biostimulant, glycine betaine is responsible for inducing tolerance to both biotic and abiotic stressors. However, the mechanism by which glycine betaine protects cool-season crops against high-temperature stress is not clear. In the present study, under the conditions of high temperatures (35 °C/30 °C day/night), cherry radishes (Raphanus sativus var. radicula Pers.) (Brassicaceae) were cultured for 9, 18, and 27 days, and different concentrations (0, 0.067, 8.79, 11.72, 14.65, and 17.58 mg L−1) of glycine betaine were applied to investigate the influence of glycine betaine on cherry radish biomass, quality, net photosynthetic rate, chlorophyll content, antioxidant enzyme activity, and endogenous hormone content under high-temperature stress. The results showed that, under high-temperature conditions, cherry radishes grew best with the 17.58 mg L−1 glycine betaine treatment. At day 27, comparing the 17.58 mg L−1 glycine betaine treatment with 0 mg L−1 glycine betaine under high-temperature stress, the cherry radish biomass increased by 44.7%, while the soluble protein and vitamin C content increased by 14.4% and 21.6%, respectively, the net photosynthetic rate and chlorophyll a content increased by 7.8% and 44.1%, respectively, and the peroxidase and catalase levels increased by 81.0% and 146.3%, respectively. On day 9, the auxin, abscisic acid, and glycine betaine contents significantly increased by 67.4%, 6.8%, and 32.9%, respectively, in comparing the 17.58 mg L−1 glycine betaine treatment with 0 mg L−1 glycine betaine under high-temperature stress. Therefore, the application of 17.58 mg L−1 betaine to cherry radishes grown under high-temperature stress had positive effects. The appropriate concentration of glycine betaine can improve the resistance of cherry radish to high temperatures and maintain yield.

Minimization of heavy metal toxicity in radish (Raphanus sativus) by strigolactone and biochar

Due to the high solubility of Cd in water, it is considered a potential toxin which can cause cancer in humans. In plants, it is associated with the development of oxidative stress due to the generation of reactive oxygen species. To overcome this issue, the roles of different plant hormones are vital. Strigolactones, one of such natural plant hormones, show promise in alleviating cadmium toxicity by mitigating its harmful effects. Acidified biochar (AB) can also effectively mitigate cadmium toxicity via ion adsorption and pH buffering. However, the combined effects of strigolactone and AB still need in-depth investigations in the context of existing literature. This study aimed to assess the individual and combined impacts of SLs (0 and 25 µM) and AB (0 and 0.75% w/w) on radish growth under Cd toxicity, i.e., 0 and 20 mg Cd/kg soil. Using a fully randomized design (CRD), each treatment was administered in four replicates. In comparison to the control under 20 mg Cd/kg soil contamination, the results showed that 25 µM strigolactone + 0.75% AB significantly improved the following: radish shoot length (~ 17%), root length (~ 47%), plant fresh weight (~ 28%), plant dry weight (~ 96%), chlorophyll a (~ 43%), chlorophyll b (~ 31%), and total chlorophyll (~ 37%). It was also noted that 0.75% AB was more pronounced in decreasing antioxidant activities than 25 µM strigolactone under 20 mg Cd/ kg soil toxicity. However, performing 25 µM strigolactone + 0.75% AB was far better than the sole application of 25 µM strigolactone and 0.75% AB in decreasing antioxidant activities in radish plants. In conclusion, by regulating antioxidant activities, 25 µM strigolactone + 0.75% AB can increase radish growth in cadmium-contaminated soils.

Pear (Pyrus communis)-based agroecosystem improves soil nutrient dynamics, microbial biomass, enzymatic activity, farm productivity and profitability

North-western Indian farmers are increasingly showing interest in adopting horticulture-based systems to enhance their farm productivity with anticipated profits. So, there is a need for systematic evaluation of different pear-based agroecosystems with respect to its productivity, profitability, soil health, and biodiversity to tackle the problems of modern agriculture (food security, declining soil health, and sustainability). Therefore, an intercropping experiment comprising 11 treatments viz. [T1: Pear (Pyrus communis) + Pea (Pisum sativum), T2: Pear + Radish (Raphanus sativa), T3: Pear + Coriander (Coriandrum sativum), T4: Pear + Kasuri fenugreek (Trigonella corniculata), T5: Pear + fenugreek (Trigonella foenum-graecum), T6: Pear + Spinach (Spinacia oleracea), T7: Pear + Green onion (Allium fistolosum), T8: Pear + Cauliflower (Brassica oleraceae var. botrytis), T9: Pear + Toria (Brassica rapa), T10: Pear + Knol khol (Brassica oleraceae var. gongylodes) and T11: Pear monoculture] were laid out in randomized block design with three replications at farmer's field, Tarn Taran district, Punjab, India during 2021–22 and 2022–23. The results showed that the lesser values of bulk density (1.30 g/cm3) and higher water holding capacity (42.52%) were observed in the Pear + kasuri fenugreek intercropping system. All the legumes-based intercropping systems viz. Pear + kasuri fenugreek, Pear + fenugreek, and Pear + pea were significantly efficient in bringing enhancements to the chemical properties of the orchard's soil. Under various pear-based intercropping systems, different microbial populations (bacteria, fungi, actinomycetes, and diazotrophs) were significantly higher in legume-based intercropping systems of Pear + kasuri fenugreek, Pear + fenugreek, and Pear + pea and resulting in more enzymatic activity in the soil in terms of dehydrogenase (63.02, 61.88, and 60.48 µg TPF/g of soil/hour, respectively), alkaline phosphatase (10.05, 9.92, and 9.75 µg PNP/g of soil/hour, respectively) and urease (17.84, 17.18, 17.00 µg /g of soil/hour, respectively). The intercropping system of Pear + kasuri fenugreek significantly increased microbial biomass carbon (42.20%) and microbial biomass nitrogen (65.02%) over Pear monoculture. The correlation analysis of various soil's physiochemical and biological parameters indicated significant associations among themselves at p = 0.01, 0.05. The intercropping system of Pear + kasuri fenugreek also produced significantly higher system productivity in early (122.20 kg/ha/day in 2021–22 and 123.86 kg/ha/day in 2022–23) and main season (119.08 kg/ha/day in 2021–22 and 121.60 kg/ha/day in 2022–23) due to significantly higher pear yield (340.02 q/ha in 2021–22 and 341.89 q/ha in 2022–23) and higher pear equivalent yield (105.99 in early season, 94.64 q/ha in main season of 2021–22 & 110.20 in early season, 101.94 q/ha in main season of 2022–23) observed by kasuri fenugreek, resulting in higher net returns, BC ratio and economic efficiency in both seasons during both years over other intercropping systems.

Effects of Different Sources of Fertilizers on Growth and Yield of Radish (Raphanus sativus L.)

Radish is a widely cultivated root crop susceptible to chemicals for its growth and development. The experiment was conducted to study the effect of different sources of organic fertilizers on growth and yield parameters in the Tokinashi variety of radish. The experiment was laid out using one factorial completely randomized block design with six treatments replicated four times. Vermicompost, goat manure, chemical fertilizers (NPK), poultry manure, farmyard manure and control were the applied treatments. Various growth and yield parameters of radish were measured and assessed at 15-day intervals. Various growth and yield parameters of radish were measured and assessed at 15-day intervals. Growth parameters were observed at 15, 30, 45, and 60 days after sowing. For yield parameters, destructive samples were taken and observed at 30 DAS and 45 DAS and harvested at 60 DAS. The assessments of plant growth and yield parameters showed that poultry manure (PM) treatments were superior. At the time of harvesting, the treatment comprising poultry manure had considerably greater plant height (30.55 cm), leaf length (29.44cm), leaf breadth (10.78cm), leaf number per plant (9.23), root diameter (10.33cm) and root length (20.16cm) with superior yield per ha (33.59 mt ha-1). Poultry manure significantly enhanced root yield and was positively correlated with all growth parameters. The highest benefit-cost (B: C) ratio was recorded in chemical fertilizer (3.99), which was statistically at par with poultry manure (3.81), suggesting that poultry manure might potentially replace chemical fertilizers for the Tokinashi variety of radish cultivation.

Effect of high static electric field on germination and early stage of growth of Avena sativa and Raphanus sativus

Presented study investigated the impact of a stationary electric field with an average value of 185 kV/m on the germination process and early growth of radish (Raphanus sativus – a eudicot plant) and oat (Avena sativa – a monocot plant). Electric field stimulation may prove to be one method to sustainably increase crop efficiency. The research is aimed to increase knowledge of the effect of a static electric field on the plant growth process, because understanding of the topic is still limited. The plants were grown on a viscose substrate in a dark room without any light. Studies have shown that the electric field can affect the germination and growth process depending on the plant species. The findings indicate a positive influence of the electric field on radish germination. The presence of the electric field accelerates the germination process and growth of young plants. On the first day of germination (the 3rd day of cultivation), about 3.2 times as many plants germinated in the samples exposed to a stationary electric field compared to the control samples. On the last day of the experiment (the 8th day of cultivation), the tallest plants in the samples subjected to the electric field were 8 % higher, compared to the tallest plants in the control samples. On the other hand, the results demonstrate a negative impact of the electric field on oat seed germination. The presence of an electric field delays the germination process and reduces the number of germinated seeds. On the last day of the experiment (the 11th day of cultivation), about 1.25 times fewer oat plants germinated in the samples exposed to a stationary electric field compared to the control samples. The tallest plants in the samples subjected to the electric field were 1.1 times smaller than the tallest plants in the control samples.