ABSTRACT Secondary metabolites have high ecological and economic values. Measuring the amounts of these metabolites in plants through laboratory analysis is time-consuming and expensive. On the other hand, the laboratory results cannot be generalized to a wide area of rangelands with diverse climates, topography, and soil. Therefore, the present study aims to evaluate the potential of PRISMA hyperspectral data to estimate the rangeland phenol concentration at landscape scale in western Isfahan province, Iran. Phenol concentration and spectral signatures were extracted from 42 plant individuals, including Astragalus adscendens, Astragalus verus, Daphne mucronata, Anabasis aphylla, and Phlomis olivieri using laboratory methods and spectroscopy in the range of 350 to 2500 nm. Phenol-sensitive bands were identified by establishing a relationship between phenol concentration and all spectral bands by the Partial Least Square Regression (PLSR) method. The map of phenol spatial changes was then produced with the Bayesian Regularized Artificial Neural Network Model (BRNN), Random Forest (RF), Generalized Linear Model (GAM), Generalized Additive Model (GLM), and Gaussian Process Regression (GPR) in rangelands. The results showed that the phenol concentration in different individuals was between 3.65 and 20.13 (mgGAE/gDW), and species spectral absorptions ranged from 1.650 to 1.660. The very high coefficient of determination of phenol concentration with spectral data (R2 = 0.94) confirmed the use of these data in phenol mapping at the landscape scale. The maximum estimated phenol concentration by GLM, GAM, RF, BRNN, and GPR models was 36.40, 26.76, 18.07, 22.74, and 32.92 GAE/g DW, respectively; which RF model had the highest (R2 = 0.955) and GLM had the lowest accuracy (R2 = 0.816). The findings show that hyperspectral data can estimate secondary metabolites at the landscape scale and can supplement laboratory methods in managing rangeland plants.