The bullet proof vest from composite fiber double ply from combination of ramie, cotton, and rayon, have been studied in order to improve the bullet proof vest in previous research using composite of pure ramie fiber. The mechanical bonding of the composite reinforced natural fiber can be increased by using chemical treatment of fiber or by using the addition of coupling agent. The chemical treatment, for example alkali treatment, is often used because it is more cost-effective. By increasing mechanical bonding also increasing the tensile strength of the composite so that this material could restrain the impact of the bullet. The result shows that the tensile strength of the composites have the optimum values for 30 minutes treated fiber in a 5% NaOH solution, i.e. 13.31 MPa, the tensile test specimens were produced according to ASTM D-638. This research is to investigate the ability of the double ply fabric itself from the bullet impact, the fiber composite and also the combination of fiber composite as a front layer (Panel A) and the double ply fiber as a second layer (Panel B) for a bullet proof vest. Optimal combination is obtained with 5 layers of panel A and 20 layers of panel B which is able to protect against cal. 7.65 mm × 17 mm (.32 ACP) bullet fired through .32 Pindad pistol from a distance of 10 meters. Panel with a size of 20 cm × 20 cm has a total thickness of 14.69 mm and a total weight of 454.73 gram. Scanning electron microscopy (SEM) observations indicated that the porosity and surface area of the fiber is smooth, fiber surfaces showed topography with microporous. SEM also showed well-arranged structure of fibers bonding. Energy Dispersive X-ray (EDX) analysis indicated 99,6% carbon contents in ramie-cotton-rayon fiber. Test result indicates that panel from composite ramie-cotton-rayon-epoxide can reach the level 1 of International Standard of NIJ-010104. Compared to panel from pure ramie fiber, the panel from this composite (0.454 kg) is lighter than previous research (0.52 kg). This combination can restrain the bullet from 10 meters distance, shorter than the previous research in 20 meters distance.