Vicia sativa L., known as common vetch, is a leguminous crop widely planted in the Eastern Mediterranean Region of Turkey. The plant is commonly grown in the region for multiple purposes such as forage in agricultural rotation systems, crop green manure, or livestock fodder. During recent disease surveys conducted in March 2019, downy mildew disease was observed in 17% of common vetch fields inspected. Typical disease symptoms were observed mostly on the leaves but often on inflorescences and stems. Local infections of the leaf lesions resulted in yellow, vein-limited blotches varying from a few millimeters in diameter to the entire leaflet on the upper surface. The lower leaf surface generally showed a greyish to purple downy covering consisting of conidia and conidiophores of the pathogen. In the case of systemic infection, nearly all of the leaves and leaflets on a given stem or branch were infected. The infected plants or branches exhibited a distorted growth characterized by dwarfing and curling of the leaflets. The infected leaflets or terminal plant parts eventually became necrotic and darkened. A representative specimen was deposited in the Korea University herbarium (KUS-F31533), Seoul, Korea. Microscopic observations revealed hyaline clusters of dichotomously branched conidiophores as emerging through stomata with up to four in fascicles. Conidiophores (n = 50) were slender, straight to slightly straight, (220 to) 275 to 475 (to 650) µm long, and branched monopodially or subdichotomously; trunks straight to slightly curved, 125 to 350 (to 450) µm, 7.5 to 10 µm wide below the first branch. Conidia (n = 50) were pale olivaceous to yellowish, ellipsoidal to broadly ellipsoidal, 25 to 30 × 18 to 23 µm, with a length/width ratio of 1.2 to 1.5. Resting organs were observed on the heavily infected leaves. Oospores (n = 50) were yellowish, broadly globose, 40 to 55 µm in diameter and had a reticulate outer wall. The pathogen was identified as Peronospora viciae (Berk.) Casp. based on the morphological characteristics (Cunnington 2006). To confirm the morphological identification, genomic DNA was extracted from conidia on freshly sporulated source plants. The internal transcribed spacer (ITS) rDNA was amplified with primers ITS1-O and LR0 and cytochrome c oxidase II (cox2) mtDNA with primers cox2-F and cox2-RC4 (Choi et al. 2015). The resulting sequences were deposited in GenBank (accession nos. MN784447 for ITS and MN792856 for cox2). BLASTn queries of NCBI GenBank revealed that each sequence is identical with ITS (EF174902, EF174934, EF174950, EF174953) and cox2 (KJ654294) sequences of P. viciae. Pathogenicity test was performed twice by inoculating the leaves of 10 healthy plants of V. sativa ‘Kocas’ (3 weeks old). Conidia were collected from fresh sporulating source plant leaves, dispersed into sterilized water with a drop of 0.1% Tween 20, and mixed to produce a conidial suspension of 10⁶ conidia/ml. Seedlings were inoculated with conidial suspension by placing several droplets onto the lower leaf surfaces. Inoculated and noninoculated plants were maintained in a growth chamber at 12/5°C, 12-h day/night cycle, and 95% relative humidity (Davidson et al. 2004). Typical symptoms of downy mildew developed on inoculated leaves after 21 days. Noninoculated plants remained symptomless. The morphological characteristics of the pathogen present on the inoculated plants were identical to the original one observed on the diseased plants, fulfilling Koch’s postulates. P. viciae was previously reported on V. sativa in several European countries (Farr and Rossman 2019). To our knowledge, this is the first report of P. viciae on V. sativa in Turkey. Both mild temperatures and frequent rains in early 2019 may have encouraged disease incidence. Because damage occurred on whole vegetative parts of the plant, the spread of this new disease agent should be monitored because it could represent a further constraint for livestock fodder production in this area.
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