This study provides a comprehensive overview of the principles of radiation protection and radiobiology. It defines and classifies radiation, distinguishing between ionizing and non-ionizing radiation. The biological effects of radiation, with a particular emphasis on its impact on DNA and cellular structures, are examined in detail. The text specifies radiation-sensitive and radiation-resistant tissues and explains both deterministic and stochastic effects. Additionally, the discussion encompasses radioepidemiological information and the relationship between linear energy transfer and DNA damage. The article delineates the classification of radiation areas as either controlled or supervised. It offers an extensive overview of the safety and protection methods employed in radiation work, including the ALARA (As Low As Reasonably Achievable) principle, the use of personal protective equipment, and dosimeters. Furthermore, it explains the radiation protection methods utilized in radiology units and outlines the precautions that should be taken during pregnancy. The article also presents recommendations for reducing radiation exposure. Overall, this article serves as a valuable resource on radiation safety for radiation workers and the public, containing essential information on protection from the harmful effects of radiation and safe working practices.