Quinoa Flour Research Articles

Enhancing emulsified sausages: a comprehensive study of the nutritional, sensory, and sustainability advantages of quinoa flour incorporation

Enhancing emulsified sausages: a comprehensive study of the nutritional, sensory, and sustainability advantages of quinoa flour incorporation

Effect of Replacing Soybean Flour by Germinated Quinoa Flour on the Physicochemical Characteristics of Vegan Burgers and Biological Activities in Diabetic Rats

Effect of Replacing Soybean Flour by Germinated Quinoa Flour on the Physicochemical Characteristics of Vegan Burgers and Biological Activities in Diabetic Rats

Improving the technology of gluten-free bread with quinoa flour

The object of this study is the technology of bread baking based on liquid sourdough and gluten-free flour mixture. The problem with this technology is that gluten-free raw materials do not contain gluten proteins, which ensure the elasticity and firmness of the dough and increase the porosity of the bread. To improve the structural and mechanical properties of the dough, new types of flour were introduced into the recipe compared to the analog recipe: quinoa, buckwheat, and oat. The positive effect of quinoa on dough quality has been known for a long time but the limitation in its use is the bitter taste caused by the content of saponins. A method of removing saponins using ultrasound (40 kHz, τ=20 min) has been proposed, which made it possible to remove 60 % of saponins and completely remove the bitterness of grains. At the same time, the content of proteins remained unchanged while the content of phenols decreased by only 0.1 mg GAE/g. The addition of 18 % (to the mass of the flour mixture) of quinoa flour and the reduction of the starch content in the recipe had a positive effect on the organoleptic parameters of the bread quality. The acidity of dough (4.4–5.2 °Н) and bread (0.6 °Н) decreased compared to the control sample, but it was sufficient for a normal fermentation process. Bread with quinoa flour had increased moisture (more than 66 %), which can negatively affect its stability during storage. An increase in the proportion of quinoa flour in the recipe by 5 % contributed to an increase in the porosity of the crumb by 2.1 %. The proposed technology for making gluten-free bread with Quartet quinoa flour based on liquid sourdough can be industrially implemented as it makes it possible to obtain a product with desirable consumer properties. The total duration of dough ripening was reduced by 1–1.5 hours, compared to the steam method, which increases the economic efficiency of the proposed technology.

Effect of whey protein on the formation, structure and gastrointestinal breakdown of quinoa flour-based composite gels

Whole grain flour has captured increasing attention due to its health benefits. Herein, the quinoa flour-based composite gels were fabricated by heating quinoa flour dispersions containing 0-7 w/w% whey protein isolate (WPI). The thermal properties and gel formation of the dispersions were determined, along with the large deformation and fracture properties, microstructure, and molecular reorganization of the resulting gels. Protein hydrolysis, solid loss, glucose release and microstructural changes were monitored to characterize the gastrointestinal breakdown of the gels. Results showed that quinoa starch was gelatinized at 60-70 °C. Upon heating, quinoa starch was segregated into microparticles of ∼ 3 μm, which aggregated into the starch phase, whereas protein aggregates filled the gap of the starch gel network. A bicontinuous structure was formed with increasing WPI concentration to 7 w/w%. Meanwhile, the storage modulus, gel hardness and fracture stress of the gels increased by 5-8 times. The addition of WPI inhibited gel disintegration during in vitro digestion. However, intestinal glucose release was not delayed because of the phase-separated structure of the gels. With increasing WPI concentration, larger protein aggregates survived after 5 min of intestinal digestion, demonstrating that the enhanced protein network was the main contributor slowing down gel disintegration during in vitro digestion. This work would promote the development of quinoa flour-based products with controlled digestion.

Modulation of textural properties in microwave treated gluten-free quinoa sponge cake by alkaline amino acids

Lysine (Lys) and Arginine (Arg) (0.6%) was added to the quinoa flour to improve the textural quality and physical properties of gluten-free sponge cake treated with different heating methods. The gluten-free sponge cake fortified Lys and Arg increased cell density and surface area fraction, compared to water bath (WB) treatment, with the microwave (MW) treatment further enhancing these effects. Furthermore, MW combined Lys treatment showed the best texture characteristics of sponge cake. The water distribution determined by 1H NMR presented that water molecules are bound by the protein network structure. FTIR spectrum proved MW combined with Lys promoted the transition from disordered structure to ordered structure of protein in quinoa sponge cake, which enhanced the expansion of protein molecules and the exposure of hydrophobic regions, thus promoting protein aggregation. SDS-PAGE revealed when alkaline amino acids were present, they can regulate the ordered aggregation of protein molecules under MW alternating electric field, leading to enhanced intermolecular interactions. Finally, microstructure evaluations by SEM demonstrated MW combined with alkaline amino acids formed a stable three-dimensional gel network structure, thus improving the quality of gluten-free quinoa sponge cake. Therefore, the study findings pointed to the feasibility of using MW to improve the physical and textual quality of quinoa gluten-free food.

Qarasiña Culinary Tradition: Conserving Quinoa (Chenopodium quinoa) as an Intangible Cultural Heritage in Jach’a Puni (Andean Community), Bolivia

Heritage encompasses features integral to the culture of a particular society, such as traditional foods that have historical significance and continue to be part of the community’s identity. These culinary traditions must be preserved to sustain cultural identity and community well-being. This study aims to document the role of qarasiña, along with other ancestral foods, in establishing quinoa (Chenopodium quinoa) as a cultural keystone species in the Jach’a Puni community. Primary data were gathered through in-depth interviews with local experts and community observations, supplemented by a comprehensive literature review. Findings reveal that in Jach’a Puni, all food is considered sacred. Three main types of qarasiña are identified: jupha qarasiña, ajara qarasiña, and kita qarasiña. The primary ingredients are domesticated quinoa (jupha) flour (Chenopodium quinoa var. quinoa) and wild quinoa (ajara) flour (Chenopodium quinoa var. melanospermum and C. hircinum). Qarasiña is integral to community banquets and social events. Although quinoa is celebrated globally as the “golden grain”, there is a risk that qarasiña may disappear and quinoa could be replaced by wheat or maize, threatening its status as a cornerstone species in the Bolivian Altiplano.

Energy-size relationship and starch modification in planetary ball milling of quinoa

The effects of speed (250–450 rpm) and time (10–50 min) on milling energy (E), particle size distribution (PSD), crystallinity degree (CD), damaged starch (DS), microstructure, hydration and pasting properties of flours obtained in a planetary ball mill (PBM) were investigated. As increasing milling severity quinoa flours showed polydispersed PSD (peaks at 417, 40 and 2 μm) with a shift towards smaller size and greater dispersion (Span: 3.0–5.2) as well as particles with rounded edges and polished surfaces (SEM images). The relationship E − D50 were satisfactorily predicted by the Walker's equation. The hydration tests as function of temperature and milling conditions revealed a complex flour behavior due to differences in PSD, composition, and DS content. The increasing milling energy caused a decrease in peak viscosity (PV), trough viscosity (TV) and final viscosity (FV) of up to 36%, 29%, and 25%, respectively. Significant correlations between flour attributes were found (DS-CD, r = −0.83, p < 0.01; PV-D50, r = 0.92, p < 0.01; TV-D50, r = 0.94, p < 0.01; FV-D50, r = 0.90, p < 0.01) denoted the increase of starch degradation as milling severity rises. These results can be used to improve the manufacture and the selection criteria of quinoa flour with specific functional attributes.

Evaluating Proximate, Antinutrient, and Antioxidant Activity of Raw and Processed Quinoa (Chenopodium Quinoa Willd) Flour and Developing Food Products by Incorporating Them

Background: This study evaluates proximate, antinutrient, and antioxidant activities of raw and processed quinoa flour (RQF and PQF). Product development and sensory evaluation were also addressed to provide prospects for human consumption. Methods: Quinoa was processed by two methods - soaking and roasting. Some nutrients like moisture, ash, protein, carbohydrate, crude fibre, fat, iron, calcium, vitamin C, phytic acid, saponins, total phenol, and 2,2-diphenylpicrylhydrazyl (DPPH) were analyzed. The recipes were made and conduct acceptability evaluation by a 9-point hedonic scale. The mean, standard deviation, and One-Way Analysis of Variance (proximate, antinutrient, antioxidant) were utilized as the statistical approach for the analysis of data. Results: The study revealed that some nutrient composition of RQF was found to be impressive with moisture (11%), ash (2.1%), protein (16.6%), crude fibre (2%), iron (11.6%), and calcium (76.1%) as compared to PQF; however, carbohydrate (69.9%), fat (9.6%), and vitamin C (9.1%) was increased in PQF, and phytic acid and saponins were decreased in all the PQF. DPPH’ free antioxidant potential was observed that RQF lowered free radical scavenging activity. In all PQF, the total phenol content was both raised and reduced. Also, proximate and antioxidant activity showed significant differences (P&lt;0.05), and antinutrient analysis showed no significant differences (P&gt;0.05). The two recipes were prepared as upma (soaked for 48 h), and chakli (roasted at 145 °C). Sample B of upma (50% semolina with 50% soaked quinoa flour) and sample A of chakli (75% refined flour with 25% roasted quinoa flour) were more acceptable. Conclusion: This study concluded that the use of domestic processing of quinoa seeds decreases some nutrient value compared with RQF and also infers the dietary importance of quinoa.

Rheological and sensory properties of chickpea and quinoa pastes and gels for plant-based product development.

The aim of this study was to investigate the modification of mechanical, rheological, and sensory properties of chickpea pastes and gels by incorporating other ingredients (olive oil or quinoa flour), to develop plant-based alternatives that meet consumer demands for healthy, natural, and enjoyable food products. The pastes and gels were made with different amounts of chickpea flour (9% and 12%, respectively). For each product, a first set of products with different oil content and a second set with quinoa flour (either added or replaced) were produced. The viscoelastic properties of the pastes and the mechanical properties of the gels were measured. Sensory evaluation and preference assessment were carried out with 100 participants using ranking tests. The study found remarkable differences in rheological, mechanical, and sensory properties of chickpea products upon the inclusion of oil and quinoa flour. The addition of oil increased the viscosity and decreased the elastic contribution to the viscoelasticity of the pastes, while it improved the firmness and plasticity in gels. It also increased the creaminess and preference of both pastes and gels. Replacing chickpea with quinoa flour resulted in less viscous pastes and gels with less firmness and more plasticity. In terms of sensory properties, the use of quinoa as a replacement ingredient resulted in less lumpiness in the chickpea paste and less consistency and more creaminess in both the pastes and gels, which had a positive effect on preference. The addition of quinoa increased the viscosity of pastes and the firmness and stiffness of gels. It increased the consistency and creaminess of both pastes and gels. Quinoa flour and/or olive oil are suitable ingredients in the formulation of chickpea-based products. They contribute to the structure of the system, providing different textural properties that improve acceptance.

Second-generation snacks prepared from quinoa with probiotic. Physicochemical properties, in vitro digestibility, antioxidant activity and consumer acceptability

Food security is a pressing issue, emphasizing the necessity for food designs that address the current geopolitical and geoeconomic challenges. This study evaluates the impact of including different percentages (10 %, 20 %, 30 %, and 60 %) of high protein quinoa flour (HPQF) in the development of a new rice-based snack. The aim is to create four snack formulations with a protein content enriched with probiotics, surpassing those currently available in the market. Probiotics Bacillus coagulans were added at a 0.1 % concentration. Once the rice flour and quinoa flour are mixed, they are mixed with the probiotic, to move on to the extrusion process. Following the incorporation of probiotics, the snacks were packaged in a modified atmosphere, and their physicochemical properties, Bacillus coagulans probiotic viability, tolerance to artificial gastroenteric juice (TAGJ), starch digestibility, and sensory acceptance were assessed.Significant differences were observed in the expansion index, with the 60 % inclusion snack exhibiting the least expansion. Despite having a higher density, this snack reached a porosity index similar to that of the 20 % HPQF snack. Achieving a 17 % protein content in the snacks was possible with a 60 % inclusion rate. Texture was notably affected by the inclusion of HPQF, with snacks having higher inclusion levels showing increased hardness. Probiotic viability evaluation consistently remained above 106 UFC/g of snack, while TAGJ exhibited a viability of 75 %. Although HPQF inclusion led to a decrease in the glycemic index (GI), snacks still maintained a GI above 70 %. Regarding antioxidant properties, snacks with 60 % HPQF inclusion displayed superior results, reaching 35.29, 5.52, and 13.74 μmol of AA/g, measured via ABTS, DPPH, and FRAP methods, respectively. These findings demonstrate a heightened antioxidant capacity compared to other formulations. Our results indicate that the new probiotic snack serves as a rich source of protein and probiotics and is well-received sensorially. However, it is worth noting that it falls within the category of high GI foods, prompting the need for future studies aimed at reducing this parameter.

Impact of genotypic variation and cultivation conditions on the techno-functional characteristics and chemical composition of 25 new Canadian quinoa cultivars

The utilization of quinoa in food production requires comprehensive information on its processing characteristics. Twenty-five new quinoa cultivars developed by the Northern Quinoa Breeding Program, grown in three Canadian locations over two seasons, were characterized for their proximate composition, pasting properties, thermal properties, water absorption index, water solubility index, foaming capacity, foaming stability, oil holding capacity, and emulsion activity crucial for potential food applications. Results showed significant variations in the proximate composition among the cultivars, which was also influenced by the growing location and harvest year. Significant differences (p < 0.05) were also observed in the pasting properties, thermal stability, hydration properties, foaming properties, oil holding capacity, and emulsion activity. The hierarchical cluster and principal component analyses were associated with five distinct clusters of quinoa cultivars, each with unique techno-functional attributes, suggesting their potential for different food applications. These findings emphasize the need for further research to explore the performance of quinoa flours in specific food products and their impact on end-product quality.

Solid-State Fermentation of Quinoa Flour: An In-Depth Analysis of Ingredient Characteristics

Plant protein ingredients are gaining attention for human nutrition, yet they differ significantly from animal proteins in functionality and nutrition. Fungal solid-state fermentation (SSF) can modulate the composition and functionality, increasing their applicability in foods. Quinoa flour (QF) served as a substrate for Aspergillus oryzae and Rhizopus oligosporus, resulting in two fermented ingredients (QFA and QFR) with different nutritional, functional, and aroma characteristics. A higher increase in protein (+35%) and nitrogen (+24%) was observed in the QFA, while fat was predominantly increased in the QFR (+78%). Fermentable oligo-, di-, monosaccharides and polyols (FODMAPs) decreased in the QFR but increased in the QFA due to polyol production. Metabolomic analysis revealed higher lactic acid concentrations in the QFA, and higher citric, malic, and fumaric acid contents in the QFR. The SSF reduced most antinutrients, while R. oligosporus produced saponins. Olfactometry showed the development of fruity ester compounds and a decrease in metallic and cardboard aromas. Both ingredients showed an enhanced water-holding capacity, with the QFA also demonstrating an increased oil-holding capacity. Complex formation increased the particle size, reduced the solubility, and decreased the foaming properties. Mycelium production darkened the ingredients, with the QFR having a higher differential colour index. This study highlights the potential of SSF to produce ingredients with improved nutritional, sensory, and functional properties.

Modulation of physico-chemical and technofunctional properties of quinoa protein isolate: Effect of precipitation acid

The typically low solubility and gelation capacity of plant proteins can impose challenges in the design of high-quality plant-based foods. The acid used during the precipitation step of plant protein isolate extraction can influence protein functionality. Here, acetic acid and citric acid were used to extract quinoa protein isolate (QPI) from quinoa flour, as these acids are more kosmotropic than the commonly used HCl, promoting the stabilisation of the native protein structure. While proximate analysis showed that total protein was similar for the three isolates, precipitation with kosmotropic acids increased soluble protein, which correlated positively with gel strength. Microstructure analysis revealed that these gels contained a less porous protein network with lipid droplet inclusions. This study shows that the choice of precipitation acid offers an opportunity to tailor the properties of quinoa protein isolate for application, a strategy that is likely applicable to other plant protein isolates.

Microwave treatment enhances the physical and sensory quality of quinoa-enriched gluten-free bread

The suitability of microwave-assisted hydrothermally treated (MWT) quinoa flour was investigated as an ingredient for enhancing the quality of gluten-free (GF) bread fortified with quinoa. Different levels (0%, 25%, 37.5%, 50%, and 75%) of native/untreated (N) or MWT quinoa flour (30% moisture content, 9 W/g, total 8 min of microwave exposure) were evaluated to replace maize starch in a starch-based GF recipe. The hydration of the dough was adjusted to obtain similar consistency in terms of complex modulus, G1*, to compensate its increase due to quinoa addition and treatment (up to +737%). The incorporation of increasing amounts of native quinoa flour reduced dough viscosimetric profiles, delayed its gelatinization peak in DSC scans (up to +3.4 °C), and decreased the dough's development and stability during the fermentation test (the final height decreased from 88 mm for 0% quinoa to 4 mm for 75%). When substituting native by MWT quinoa flour at a certain substitution level, a reduction in the breakdown viscosity, an increase in pasting temperature, and an increase in dough development and stability during the fermentation test were noted. As a result, the MWT quinoa flour-fortified breads exhibited a higher specific volume, lower hardness, and retarded staling. In addition, sensory evaluation of the breads showed a reduction in the herbaceous off-flavour associated with the quinoa (untreated) flour, and no effect on bitterness. The experimental findings indicate the feasibility of using microwave hydrothermal treatment to improve the physical properties and sensory quality of quinoa-enriched GF bread.

Assessment of functional UF- quinoa cheese properties

Quinoa flour at different ratios of 2, 4 and 6 % were used to produce UF- quinoa soft cheese treatments. Addition of quinoa improved sensory quality and other characters of quinoa soft cheese treatments than the control. For the chemical composition: TS, fat, TN, fiber and RSA values were increased. Soluble nitrogen and TVFA content were higher in UF- quinoa soft cheese treatments being the highest for that with quinoa 6 %. Viable counts of Bifidobacterium lactis and Lactobacillus casei were increased than the control. The good ability of quinoa protein to absorb water and fat help the texture forming to be smoother with no whey separation. UF- soft cheese with quinoa flour 2 and 4 % were the most palatable and preferable treatments comparing by the other treatments. All treatments stored for 28 days at (5 ± 2 °C). Three replicates were carried out for each treatment and the obtained data were statistically analyzed at p ≤ 0.05 when fresh and during storage.

Transformation of quinoa seeds to nanoscale flour by ball milling: Influence of ball diameter and milling time on the particle sizing, microstructure, and rheology

Among various size reduction techniques, high-energy ball milling is one of the most attractive means for plant-based foods. The objectives of the work were to investigate the influence of ball diameters (3, 6, and 13 mm) and milling time (2, 4, and 6 h) on particle size and microstructural properties of quinoa flours. Particle size analysis demonstrated that ball-milled particles were mostly in the range of nanoscales (122–295 nm). A longer milling time with larger balls significantly increased the particles to microscale (3.58 μm). The scanning electron microscopy displayed the conversion of quinoa starch granules into flakes after ball milling, however, the X-ray diffraction crystallinity peak observed at a 2θ value of 19–20° did not change. The AFM roughness parameters, arithmetic and squared mean heights of flours increased with increasing ball diameters. These results provided new insights for the application of ball milling, in particular in functional foods and pickering emulsion.

Nutritional and Organoleptic Characteristics of Sausage Based on an Autochthonous Ecuatorian Fish, Old Blue (Andinoacara rivulatus).

The consumption of fisheries and aquaculture products has been increasing in recent decades, and it is necessary to strike a balance between production and sustainability in aquaculture; this is essential homework to support the demand for human food. This study aimed to investigate the sensory and nutritional characteristics of a sausage made from the fillet of the native fish Old Blue (Andinoacara rivulatus) to stimulate the local economy. A multifactorial A*B*C design was used, with Factor A being rearing systems (wild and farmed), Factor B being types of protein (quinoa meal and soybean meal), and Factor C being lipids of animal (pork fat) and vegetable (sunflower oil) origin. Highly significant differences were observed in all formulas, according to Tukey (p < 0.05). The highest protein percentage was with soybean flour at 11.24%, while quinoa flour had 10.80% of the product. In sensory characteristics, the best attributes were for texture in the mouth with a hedonic scale from 0 to 5, with 4.2 firmness being mostly acceptable, odor at 4.5, the aromatic attribute with the highest and best value, and color was the clearest at 4.3 acceptability. The flavor was 4.3, mostly more pleasant according to the tasters. The yield of farmed fillets was 23.16% compared to wild 13.89%, and the balance of sausage yield was 393 g with a yield of 76.33% of the total weight. Microbiological analysis of the sausage with the native species showed the absence of pathogenic microorganisms. A commercial shelf life of 30 days was also established for the sausage, demonstrating an added value through this processing, allowing its exploitation in areas further away from the fishing sites and, thus, a greater possibility of social development in the area.

Effect of Different Hydrocolloids on the Qualitative Characteristics of Fermented Gluten-Free Quinoa Dough and Bread.

The aim of this research was to optimize the production process of fermented gluten-free quinoa bread. To this end, the effect of different hydrocolloids on the technological, fermentative, and nutritional properties of quinoa-based gluten-free doughs and breads was evaluated. For this purpose, 3% of four different hydrocolloids (sodium alginate, k-carrageenan, xanthan gum, and hydroxypropyl methylcellulose (HPMC)) were used in gluten-free doughs composed of 50% quinoa flour, 20% rice flour, and 30% potato starch. The rheological and fermentative properties of the doughs were evaluated, as well as the chemical composition, specific volume, crust and crumb color, and alveolar structure profile of gluten-free breads. The results highlighted the differences in dough rheology during mixing and fermentation of the doughs. In particular, HPMC showed a good gas retention (93%) during the fermentation of quinoa dough by registering the highest maximum dough development height (Hm). The gluten-free quinoa breads obtained were characterized by significantly different quality parameters (p < 0.05). The use of 3% HPMC resulted in breads with the lowest baking loss, the highest volume, and the most open crumb structure.

Evaluation of Physical Characteristics and Impact of Germination on Functional Properties of Quinoa (Chenopodium quinoa Willd.)

Background: Quinoa is a nutrient-dense pseudocereal with remarkable nutritional quality. It is a gluten-free crop that provides a balanced set of essential amino acids and possesses hypoglycemic and free fatty acid-lowering properties. Quinoa has gained popularity in the current era due to its tolerance to extreme weather conditions, nutritional composition and other industrial applications. Methods: The present study analyzed the physical and functional properties of quinoa using standard procedures and assessed the impact of germination on these properties. Germinated flour was developed by soaking quinoa seeds overnight and allowing for germination for 72 hours in an incubator at 20±2°C at 80-90% humidity. Germinated seeds were oven-dried at 60±2°C for 45 minutes, followed by grinding into flour and storing the flour in a dry and air-tight container for further analysis. Result: The study findings showed that quinoa seeds had good physical properties. All functional properties viz water absorption capacity, oil absorption capacity, emulsion and foaming properties of quinoa flour improved on germination except flour solubility. It is concluded that germination is a cost-effective processing technique for enhancing the functional properties of quinoa flour, so as to increase its industrial use for product development. Germinated quinoa flour can be utilized in the development of various infant formulas.

Influence of Soy Protein Hydrolysates on Thermo-Mechanical Properties of Gluten-Free Flour and Muffin Quality

The influence of protease-assisted hydrolysis on the impact exerted by the soy protein isolate on the thermo-mechanical behavior and baking performance of the gluten-free composite flour, consisting of a mixture of rice and quinoa flours, was investigated. The mPAGE analysis revealed that soluble fractions of the hydrolysates, obtained with bromelain, Neutrase or trypsin, concentrated the peptides with a molecular weight lower than 20 kDa, whereas the insoluble ones retained higher molecular weight fragments. The influence of the separate and cumulative addition of the soluble and insoluble soy peptide fractions on the thermo-mechanical properties of dough was tested by means of a Mixolab device. Regardless of the enzyme used for hydrolysis, the addition of the soluble peptide fraction to the gluten-free composite flour resulted in delayed starch gelatinization, whereas the insoluble one caused a considerable increase in the dough consistency. The most important improvements in the dough behavior were observed when supplementing the gluten-free flour with 10% soy protein hydrolysates obtained with bromelain and trypsin. The gluten-free muffins enriched in soy protein hydrolysate exhibited important differences in terms of moisture, height and specific volume, compared to the control. Moreover, the ABTS- and DPPH-based methods indicated that protein hydrolysate addition caused a significant improvement in the antioxidant activity (by at least 38% and 23%, respectively) compared to the control. In conclusion, soy protein hydrolysate might be successfully used for increasing both the protein content and the antioxidant activity of the muffin samples.