Primary stability of a dental implant is defined as its ability to resist the applied load without showing excessive damage in peri-implant bone, which is a prerequisite for secondary stability, and consequently for implantation success. The main goal of this study was to develop a validated micro-finite element (μFE) approach to assess the primary stability of dental implants in terms of stiffness, stiffness reduction, and irreversible displacement of the bone-implant system, subjected to an increasing step-wise quasi-static compressive loading-unloading test. The μFE models were generated based on the μCT images of bone, taken from extracted bovine tibia trabecular bone samples after drilling and implantation. A tissue constitutive model was considered for trabecular bone by describing elasto-plasticity with a modified von Mises yield criterion and element deletion technique to account for trabecular bone damage behavior. Then, the obtained force-displacement curves from the simulation were compared with the in-vitro mechanical test curves to evaluate the validity of the model. The results showed that the proposed μFE model could be properly predict the bone-implant system mechanical response in terms of irreversible displacement (R2 = 0.99), stiffness (R2 = 0.77), and stiffness reduction (R2 = 0.72) of the bone-implant construct for all the applied displacements without a significant difference from the unit slope and zero intercept of the QQ-plot (p-value<0.05). Moreover, a qualitative agreement was seen between the peri-implant bone damage predicted by the μFE model and the observed from μCT images. The adopted methodology used in this study can predict the mechanical failure response of the bone-implant system, which can be employed as a representative tool to study the effects of various dental implant design parameters on the primary stability with the ultimate goal of optimizing dental implants design.