Amount Of Nitrogen Research Articles

Evaluation of the Fertilizer Potential of Black Soldier Fly Larval Frass Obtained by the Processing of Multiple Organic Wastes in Agriculture in Cameroon: Effects on Plant Growth Parameters and Maize Kernel Nutrient Composition

The growing utilization of inorganic fertilizers results in the depletion of a minimal quantity of essential elements, including phosphorus, nitrogen, and potassium. The residual fraction is discharged into the environment as organic waste, posing a threat to the ecosystem.The black soldier fly larvae (BSFL) have been acknowledged as a solution for the increasing problem of organic waste. BSFL provides numerous benefits, including their current use of waste material, known as frass, as an organic fertilizer. The aim of this study is to examine the fertilizing properties of frass and its effects on plant growth parameters and nutrient concentration in maize kernels when consumed. This will be compared to other cultivation methods including synthetic fertilizers, conventional compost, and no compost. Following a 90-day timeframe, a comprehensive analysis was performed to assess the growth characteristics and nutrient composition within the kennels. The results of this study indicate that frass positively affects plant growth and has a favorable blend of nutrients, as previously demonstrated by studies investigating the nutrient levels in maize. This study showcased the capacity of utilizing frass as a carrier to boost agricultural output and enhance the accessibility of particular nutrients in plants. Further investigation is required to substantiate these conclusions. The widespread use of black soldier fly larvae in the agricultural sector in poor countries, particularly in Sub-Saharan Africa, has the capacity to have a beneficial effect on integrated sustainable agriculture and the achievement of Sustainable Development Goals 2, 11, and 12.

Nutrient Use Efficiency and Cucumber Productivity as a Function of the Nitrogen Fertilization Rate and the Wood Fiber Content in Growing Media.

A peat substrate is made from peat from drained peatlands, which is a limited resource. A realistic estimate is that 50% of the world's wetlands have been lost. Peat is used in horticulture, especially for the cultivation of vegetables in greenhouses. The consequences of peatland exploitation are an increase in the greenhouse effect and a decrease in carbon stocks. Wood fiber can be used as an alternative to peat. The chemical properties of growing media interact and change continuously due to the small volume of growing media, which is limited by the growing container. This study aims to gain new knowledge on the impact of nutrient changes in the microbial degradation of carbon compounds in wood fiber and mixtures with a peat substrate on the content and uptake of nutrients required by plants. The cucumber (Cucumis sativus L.) variety 'Dirigent H' developed in the Netherlands was cultivated in growing media of a peat substrate and wood fiber: (1) peat substrate (PS); (2) wood fiber (WF); (3) wood fiber and peat substrate 50/50 v/v (WF/PS 50/50); (4) wood fiber and peat substrate 25/75 v/v (WF/PS 25/75). The rates of fertilization were the following: (1) conventional fertilization (CF); (2) 13 g N per plant (N13); (3) 23 g N per plant (N23); (4) 30 g N per plant (N30). The experiment was carried out with three replications. As the amount of wood fiber increased, the humidity and pH of the growing media increased. The fertilization of the cucumbers with different quantities of nitrogen influenced the nutrient uptake. The plants grown in the 50/50 and 25/75 growing media had the best Cu uptake when fertilized with N23. When the plants grown in the wood fiber media and the 50/50 media were fertilized with N13, N23, and N30, the Mn content in the growing media at the end of the growing season was significantly lower than the Mn content in the media with conventional fertilization. Thus, nitrogen improved the uptake of Mn by the plants grown not only in the wood fiber, but also in the combinations with a peat substrate. Growing plants in wood fiber and fertilizing them with N13 can result in the optimum uptake of micronutrients. The number and biomass of cucumber fruits per plant were influenced by the amount of wood fiber in the growing media and the application of nitrogen fertilizer. The highest number of fruits and biomass of fruits per plant obtained were significantly higher when the cucumbers were grown in WF/PS 50/50 growing media with additional N13 fertilization.

Unveiling nutraceutical, antioxidant properties and bioactive compound profiling of Chironji gum

Plant gums had great significance from ancient times because of their medicinal qualities and bioavailability due to their wide uses such as ethnomedical purposes, culinary, pharmaceutical, paper, textile, and other sectors. Chironji is a natural gum-producing underutilized, and native crop to India, and its pulp and kernel contain high nutraceutical value. The Chironji plant-produced gum might have some nutraceutical value. Hence, in the present study, morphological, physical, and biochemical parameters of gum were analyzed. The result of this study revealed that Chironji gum contains a fair amount of total polysaccharide (36.05 %), total protein (22.6 %), total nitrogen (3.61%), and total amino acid (20.4%). Through UHPLC-Q-TOF-MS analysis, different bioactive compounds namely 1,2- aminoalcohols, acyl monophosphates, amino cyclitol, aromatic amide, polychlorinated biphenyl, chlorohydrin's, depsipeptides, pyridines, terpene glycosides, alkaloid, etc, are profiled in Chironji gum. Apart from these physicochemical properties, the development of safety profiles is also required to be a part of pharmaceutical use.

Photocatalysis, wettability and optical properties of N-doped Cu2O/CuO thin films for smart coating applications

Abstract Undoped and nitrogen (N)_doped Cu2O/CuO thin films were deposited via reactive DC magnetron sputtering. The deposition was carried out by sputtering the Cu targets under various Ar/N2/O2 gas flow ratios. The structural, optical, wettability, and photocatalytic performance of the deposited films were investigated. A simple cubic Cu2O crystallographic phase is observed for the undoped film, whereas mixed cubic Cu2O and monoclinic CuO phases (Cu2O/CuO) are observed for the N_doped films. EDAX revealed that as the N2 flow rate increased the amount of nitrogen incorporated into the film increased. The transmittance and reflectance are affected by the incorporation of nitrogen into the films. The transmittance values decreased with increasing N2 flow rate, whereas the reflectance values increased. Both the refractive index and extinction coefficient almost increased with increasing N2 flow rate. A noticeable optical band gap narrowing from 2.55 eV to 2.39 eV was detected upon increasing the N2 flow from 0.0 to 190 sccm. The photoluminescence spectrum of the undoped sample contains five distinct bands at 518, 612, 654, 714 and 825 nm. These five maxima are attributed to the radiative decay of bound and free excitons, and oxygen vacancies (VO) After nitrogen incorporation, the photoluminescence intensity decreases and then increases again with increasing N2 flow rate. A reduction in the water contact angle was observed with increasing N2 flow rate. Upon Vis-light illumination, the N_doped Cu2O/CuO films reached superhydrophilicity faster than the undoped film did. The photocatalytic performance of the deposited Cu2O/CuO films was strongly enhanced with a small amount of N doping. The deposited films are promising for self-cleaning and photocatalytic degradation of organic wastes.

Effect of Sowing Dates and Flower Pruning on Chemical Properties and Shelf Life of Yam Bean (Pachyrrhizus erosus L.) Tubers

Underutilized crops provide essential micro-nutrient and thus able to complement staple foods. The inhibitory effect of floral and fruit development may affect the tuber development. It is logical to assume that large amounts of nitrogen and other essential components are assimilated by the reproductive parts of plant, hence it is important to remove these reproductive parts as well as time of sowing also affect the shelf life and chemical properties of yam bean tubers which form the basis of an experiment on effect of sowing dates and flower pruning on chemical properties and shelf life of Yam bean tubers. An experiment was conducted at Department of Vegetable Science, College of Horticulture, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli during the year 2023-24. Twelve treatments and 3 replications in Factorial Randomized Block Design Planting dates (D) i.e., D₁-1st week of June, D₂-3rd week of June, D3 -1st week of July, D4 -3rd week of July and Interval of reproductive pruning (P) i.e., P1 – Weekly, P2 – Fortnightly, P3 – No pruning. Chemical analysis of the tubers was done to analyse the starch, fiber, dry matter, TSS and shelf life of the tubers from various treatments. Investigation revealed that among the various chemical parameters and shelf life the effect of interaction on starch, fiber, dry matter and shelf life was significant whereas it was non-significant on TSS. The highest starch content was recorded in D3P1 (6.81%). The fiber content was analysed highest in D4P3 (0.69 %). The highest dry matter content was analysed in D1P3 (24.59 %). The highest TSS was recorded in D1P1 (6.40 °B) and highest shelf life was recorded in D1P1 (36.56 days). Considering its nutritional quality and sustainability in the region it can be an alternative crop with food security, nutritional security as well as economic sustainability [1].

Effect of Microplastics and Straw Addition on Nitrogen and Phosphorus Leaching in Orange Orchard Soils

To explore the effects of microplastic input and straw addition on nitrogen and phosphorus leaching in orange orchard soil, indoor soil column leaching simulation experiments were conducted on orange orchard soil in Dangyang City, Hubei Province. The experiment analyzed the effects of different treatments on the leaching characteristics of soil nitrogen and phosphorus. The results showed that： ① The increase in microplastic input increased the leaching capacity of the soil （TN, NO3--N, NH4+-N, and TP） when only microplastic input was added. ② Under the addition of straw, the input of microplastics increased, which reduced the leaching amount of TN and NO3--N in the soil, and increased the leaching amount of NH4+-N and TP in the soil. ③ Under the input of microplastics, the key influencing factors of soil nitrogen and phosphorus leaching were soil bulk density and water content. Under straw addition, it was mainly affected by soil carbon and nitrogen content. Among them, microplastic input and straw addition significantly increased soil pH, but their path coefficients for nitrogen and phosphorus leaching were not significantly correlated. The results showed that the effects of polypropylene microplastic input and straw addition on soil nitrogen and phosphorus leaching were related to the amount of microplastics input and whether straw was added. The results showed that the input of microplastics would increase the amount of soil nitrogen and phosphorus leaching. Although nitrogen leaching loss caused by microplastic input could be reduced under straw addition, soil phosphorus leaching loss was significantly increased.

Effects of Different Nitrogen Application Measures on N2O Emissions in Wheat-maize Rotation System

To investigate the response of N2O emissions from farmland soil to different nitrogen application measures and the factors affecting it in the wheat and jade rotation system in North China, we analyzed the results of the one-time application of fertilizer in the following six treatments： without fertilization （CK）, conventional nitrogen application （urea, one instance of follow-up fertilization, U1）, optimized nitrogen application （20% nitrogen reduction, one instance of follow-up fertilization, U2）, one-time fertilization of controlled-release doped fertilizers （50% urea + controlled-release urea 50%, nitrogen reduction 20%, SRU1）, controlled-release fertilizer one-time fertilization （nitrogen reduction 20%, SRU2）, and inhibitor-type controlled-release fertilizer one-time fertilization （nitrogen reduction 20%, ISRU）, and the differences in N2O emission fluxes and soil physicochemical properties were determined. The results showed that soil N2O emission fluxes were dynamic during the wheat and jade crop rotation, and the N2O emission peaks of the CK, U1, and U2 treatments appeared in the corn season at 4-7d of basal fertilizer and 6-10d of fertilizer, and out of the wheat season its emission peaks appeared in the basal fertilizer at 4-8 d and 6-9 d of fertilizer. The emission peaks of SRU1, SRU2, and ISRU appeared in the basal fertilizer at 10-21 d, 12-20 d, and 12-20 d of fertilizer, respectively, in the corn season and the wheat season. At 21 d and 12-20 d, the application of controlled-release fertilizer significantly reduced the peak and frequency of N2O emission. Compared with those in U1, the N2O emission fluxes of the U2, SRU1, SRU2, and ISRU treatments were significantly reduced by 8.5%, 20.0%, 33.8%, and 43.6%, respectively, and the N2O emission fluxes were higher in the corn season than in the wheat season, which accounted for 58.1%-65.1% of the whole crop rotation cycle. Yield was reduced by 5.9% and 1.9% for the U2 and SRU1 treatments and increased by 1.7% and 7.0% for the SRU2 and ISRU treatments, respectively, compared to that in U1. In the maize season, the ISRU yield increased by 14.6%, the environmental benefit was $581, and the net economic benefit increased by up to 18.6% compared to that in U1, whereas the wheat season showed a decrease in yield and net economic benefit, which resulted in a one-time application of the inhibitor controlled-release fertilizers being more suitable for the maize season. Correlation analysis of N2O emission fluxes with the physicochemical indices of soil properties revealed that N2O was correlated with moisture, ammonium N, nitrate N, and microbial carbon and nitrate N and nitrogen. N2O and moisture, ammonium N, nitrate N, and microbial amount of carbon and nitrogen were significantly positively correlated. Thus, the one-time application of inhibitor-type controlled-release fertilizers played a positive role in reducing labor inputs and environmental benefits, and it could be an effective method of N fertilizer management for maize cultivation in North China.

Effect of mineral additives and ammonia-oxidizing bacteria additions on physicochemical properties, nitrogen retention and microbial dynamics during vermicomposting of chicken manure and green waste

Vermicomposting is an effective technology to transform organic wastes into organic fertilizers. However, a large amount of nitrogen will be lost through ammonia (NH3) volatilization during verimicomposting. Herein, we investigated the effects of mineral additives (diatomite, zeolite) and exogenous ammonia-oxidizing bacteria (AOB) (Nitrosomonas europaea, Nitrosospira multiformis) added individually or combinedly on the physicochemical changes, the conversion of nitrogen, and the resident microbial community succession during chicken manure and green waste co-vermicomposting. The results showed that the combined addition of AOB and mineral additives significantly improved vermicompost quality and maturity in terms of humus, carbon/nitrogen ratio, humic acid/fulvic acid ratio and germination index. Compared with the control, jointly adding AOB and mineral additives reduced NH3 emissions by 65.72∼71.05 %, accelerated the conversion of NH4+-N to NO3−-N, increased total nitrogen content by 23.22∼25.21 %. The combination of AOB and mineral additives significantly increased the activities of ammonia monooxygenase, hydroxylamine oxidase, and nitrate oxidoreductase, and enhanced the amount of AOB community. Additionally, the combined addition of AOB and mineral additives increased bacterial richness and diversity, accompanied by increased relative abundances of Luteimonas, Cellvibrio, Saccharimonadales, and Pseudomonas. These microbial shifts were correlated with enhanced lignocellulose degradation, organic phosphate mineralization, and promotion of plant growth. Moreover, vermiompost-based growing media with mineral additives and AOB can significantly promote the growth of purple cabbage as compared to the vermicompost without additives. Overall, the synergistic use of AOB and mineral additives (diatomite, zeolite) was recommended as a favorable approach for reducing nitrogen losses and improving the efficiency of chicken manure and green waste vermicomposting. This study offers a novel approach to mediate nitrogen transformation processes and reduce nitrogen loss.

Long lasting degradation of all alkanes in soil by Pseudomonas activated after Fenton pre-oxidation

This study investigated the function and mechanism of Fenton pre-oxidation on the long lasting degradation of all alkanes in soil contaminated by petroleum. The findings demonstrated that the biological removal amount of all alkanes in the respiratory regulation group reached 4083.46 mg/kg, which was twice that of the non-regulation group, and the removal amount gradually increased in the four stages of bioremediation. In addition, the removal amount of all alkanes in the non-regulated group did not change much and showed a downward trend, indicating that long lasting degradation of all alkanes could be achieved by the respiratory regulation group, and the biodegradation cycle was saved by 251 days compared with the non-regulated group. Furthermore, the total number of bacteria in the respiratory regulation group (6.73 log CFU/g) was significantly higher than that in the non-regulation group (2.25 log CFU/g). Pseudomonas became the dominant genus in the respiratory regulation group with an average relative abundance of 32.17 %. In the respiratory regulation group, a large amount of ammonia nitrogen (1703.62 mg/kg) was consumed during the degradation process, which stimulated the tricarboxylic acid cycle respiratory metabolism process of Pseudomonas and accelerated the hydrocarbon conversion. This may be the reason why the long lasting degradation of all alkanes in soil could be achieved by the respiratory regulation group.

Effect of integrated nutrient management on the growth and yield of YellowSarson (Brassica rapa var. yellow sarson) under guava (Psidium guajava) basedagri-horti system

An experiment was conducted during the winter (rabi) season of 2021–22 to evaluate the effect of integrated nutrient management on the growth and yield of yellow sarson under guava based agri-horti system. The field ex- periment comprised 8 treatment, viz., T1 , Control; T2 , 100% RDF + 30 kg S + 5 kg Zn; T3 , 50% RDF + 15 kg S + 2.5 kg Zn + 2.5 t/ha FYM+ 2 spray of nano urea; T4 , 50% RDF + 15 kg S + 2.5 kg Zn + 5 t/ha FYM+ one spray of nano urea; T5 , 75% RDF + 22.5 kg S + 3.7 kg Zn + 2.5 t/ha FYM + 2 spray of nano urea; T6 , 75% RDF + 22.5 kg S + 3.7 kg Zn +5 t/ha FYM + 1 spray of nano urea; T7 , 100 % RDF + 30 kg S + 5 kg Zn + 2.5 t/ha FYM; T8 , 100% RDF + 30 kg S + 5 kg Zn + 5 t/ha FYM, was laid out in randomized block design, with 3 replications. The results revealed that application of 75% RDF + 22.5 kg S + 3.7 kg Zn +2.5 t/ha FYM + 2 spray of nano urea at 30-35 DAS and pre-flow- ering stage (T5 ) gave significantly higher growth and yield attributes such as plant height (141.03 cm), dry matter accumulation (37.76 g/plant), no. of siliqua (142/plant), siliqua length (6.67 cm), test weight (3.43 g) as well as seed yield (1.49 t/ha) and stover yield (3.54 t/ha) as compared to control. The highest amount of nitrogen, phos- phorus, potassium and sulphur uptake by plant was also observed in the treatment T5. These findings provide valuable insights for farmers and researchers aiming to optimize nutrient management strategies and improve crop productivity in similar agri-horti systems.

The influence of nitrogen content on the microstructure and properties of W-Ta-Cr-V-N refractory high-entropy nitrides

To enhance the mechanical performance and corrosion resistance of refractory high-entropy alloys (RHEAs) and to identify materials better suited for severe high-temperature and corrosive environments, this study utilized a double glow plasma surface alloying technique to fabricate a W-Ta-Cr-V-N refractory high-entropy nitride coating on a W substrate. The microstructure and phase structure of the samples were characterized using a scanning electron microscope and X-ray diffractometer. The mechanical properties of the samples were evaluated through microhardness testing, while their corrosion performance was assessed by potentiodynamic polarization and electrochemical impedance spectroscopy measurements. The study investigated the effects of different nitrogen contents on the microstructure, mechanical properties, and corrosion resistance of the BCC-structured W-Ta-Cr-V alloy in the past tense. The study found that the doping of an appropriate amount of nitrogen can lead to the formation of FCC and HCP structured nitrides. As the nitrogen content increases, there is a significant change in the preferred orientation of the coating. When the nitrogen content is 16.6 at.%, the distribution of the nitrides is uneven, resulting in increased surface undulations and roughness. Conversely, when the nitrogen content is elevated to 48.1 at.%, the clustering of surface nitride particles becomes more pronounced, leading to a decrease in the coating density. The thickness of the nitride coatings is around 10 μm, with a slight decrease in thickness as the nitrogen content increases. The hardness of the coatings is significantly superior to that of the W-Ta-Cr-V alloy films, reaching up to 3465 HV0.25 in the highest instance. The introduction of 31.4 at.% nitrogen results in the densest coating, effectively enhancing the corrosion resistance of the refractory high-entropy alloy. Therefore, the doping of an appropriate amount of nitrogen can alter the microstructure of the refractory high-entropy alloy, improve its mechanical properties, and enhance its chemical stability.

Effect of welding gas mixtures on weld material and bead geometry

Abstract Shielding gas is an essential parameter in welding processes, serves to shield the molten material from the atmosphere and external contaminations while providing a stable electric-arc pathway during the process, thus influencing the quality of the weld bead and several other aspects of the process. Therefore, any welding-based process, such as wire-arc additive manufacturing, could benefit from shielding gas mixture optimisation to selectively improve the processing and possibly the final mechanical properties of the welded material. In this study welding of EN AW 5183 aluminium (single weld bead and wire-arc additive manufacturing build-up) was conducted using several gas mixtures and their influence on final geometry defects, and microstructure measured. The gas mixtures used were a combination of varying amounts of carbon dioxide, nitrogen, and oxygen. These were added to commercially available argon gas. It was observed that: CO2 additions led to an unfavourable bonding defect and increased mean grain size; nitrogen additions caused a decrease in mean grain size, decreased pore count while maintaining a similar pore volume fraction to the reference argon mixture samples; oxygen additions showed slight reduction of mean grain size, decreased pore count and decreased pore volume fraction. These results further showcase that varying shielding gas mixtures influence the final material properties and should be considered as an additional improvement route for conventional welding and additive manufacturing.

Enhancing pipe chilldown with axial grooves filled with silicone sealant

This study proposes a method to accelerate the chilldown process in pipes using liquid nitrogen flow. Axial grooves were machined along the inner surface of the pipe and then filled with silicone sealant. This approach achieves a shorter chilldown time compared to previously proposed methods. Initial experiments involving pool boiling in liquid nitrogen were conducted to determine the optimal groove width and the ratio of groove-to-sealant area. The shortest chilldown time in pool boiling was achieved when the groove pitch was close to the capillary length. The effect of the copper-to-silicone sealant area ratio on the chilldown process was minimal. The shortest chilldown time was achieved with a surface that had a 2 mm pitch and a copper-to-silicone sealant area ratio of 0.25. The chilldown time was 1/4.4 of that of the bare surface. Pipes with different pitch and groove widths were tested in a flow chilldown experiment. Using the surface texture, the chilldown time of a stainless-steel pipe with an outer diameter of 1/2″ and a length of 120 mm from room temperature to the saturation temperature was reduced to a maximum of 1/3.6. The shortest cooling time is obtained at a groove pitch of 2 mm. In addition to the shorter cooling time, the amount of liquid nitrogen required for chilldown was also reduced.

Nitrogen overfertilization: Impact on ecological interactions and floral morphology in a peri‐urban environment

Abstract Nitrogen is a crucial element that affects plant metabolic processes and indirectly influences the structure and dynamics of ecological interactions in terrestrial ecosystems. Since the invention of the Haber–Bosch process, human activity has significantly influenced the amount of nitrogen released into the environment. However, how do these overfertilization processes affect flora and fauna in urban ecosystems? In our experiments, we used elevated nitrogen concentrations to simulate terrestrial eutrophication processes in Nicotiana tabacum L. We evaluated reproductive traits such as floral morphology using geometric morphometrics. Additionally, nectar volume and solute concentration, along with other vegetative traits, were quantified. Subsequently, the impacts of these modifications on herbivory and pollination interactions in a peri‐urban environment were measured. Overfertilization resulted in notable modifications to the floral shape of the lobes, as well as a significant increase in percentage of herbivory by chewing insects, without affecting floral visitation rates. We also observed that elevated herbivory on fertilised plants did not show a direct correlation with overall pollination rates per plant or seed set. In contrast, the reduction in leaf size during the second phase of the experiment, owing to overfertilization effects, affected seed production. The roles of potential pollinators and their influence on seed quantity in control plants are discussed. Nitrogen is a crucial element not only for plants in natural terrestrial ecosystems but also plays a fundamental role in the modification and shaping of reproductive and vegetative structures. Moreover, it affects the functionality of ecological interactions of pollination and herbivory in anthropogenic environments, such as peri‐urban areas.

Investigating the Synergistic Effects of Using Nitrogen and Sulfur Fertilizers on the Growth of Safflower (<i>Carthamus tinctorius</i> L.)

Nutrient imbalance is one of the prevalent problems in the semi-arid region. The study aimed to investigate the effects of sole and simultaneous application of nitrogen and sulfur on safflower yield in a semi-arid region. A field trial was designed to explore the combined use of sulfur (S0: control, S1 and S2: 25 and 50 kg ha-1 S via sulfur phosphate composites, S3 and S4=: 25 and 50 kg ha-1 of S via elemental sulfur, S5 and S6: 25 and 50 kg ha-1 of S via zinc sulfate) and nitrogen (0, 40 and 80 kg ha-1) on the growth and seed production of safflower (Carthamus tinctorius L.) in a west of Iran with mesic active calcixerepts soil that had medium to low fertility. The utilization of large amounts of nitrogen greatly increased the height of the first pod from the ground surface (P ≤ 0.05), and the highest amount was recorded in S 6+ N80 conditions (65%). Leaf chlorophyll content was significantly affected by nitrogen consumption (P ≤ 0.01) and the highest chlorophyll was recorded in S4, S5, and S6 treatments having high levels of nitrogen. A similar trend was observed for the canopy width and the application of nitrogen, zinc sulfate, and elemental sulfur could increase this component by 33%. The highest number of heads was achieved with the use of S5 + N80 and S2 + N80, which was 15% more compared to the control. The application S2 + N80 increased seed number in capitulum by 25% over the control. The application of S along with high levels of nitrogen improved the seed yield by 45% (1334 ± 78.35 kg ha-1). However, only the application of sulfur could improve the harvest index (P ≤ 0.05). The results indicated that applying moderate to high amounts of zinc sulfate and elemental sulfur, in combination with N80, enhanced safflower growth characteristics and improved seed yield components, likely due to synergistic interactions between the elements. Altogether the use of zinc sulfate or elemental sulfur as a logical and effective management solution can increase the efficiency of nitrogen fertilizer and improve nutritional balance.

Assessment of Physico-Chemical Properties of Biogas Slurry as an Organic Fertilizer for Sustainable Agriculture

Chemical fertilizers have been extensively used for growing crops and controlling plant diseases, but they pose potential hazards to the environment, soil health, plants, and people. The current world situation highlights the need to implement eco-friendly agricultural practices for sustainable crop production. Using environmentally friendly manure, such as biogas slurry, can help reduce the negative effects of chemical fertilizers. Biogas slurry is an efficient waste material and organic fertilizer, making it an ideal supplement for sustainable crop production and waste management. An experiment was conducted at IARI, New Delhi, to explore the nutrient potential of biogas slurry. The main objective of this study was to assess biogas slurry's physico-chemical characteristics and nutrient contents. Samples of biogas slurry were collected in three replications and analyzed using standard methods for macro and micronutrients. The data revealed that biogas slurry has a pH of 7.2-8.5, EC of 1.06 to 1.12 dS/m, and organic carbon content of 41.7 to 45.8%. In terms of fertility, it contains significant amounts of nitrogen (1.98-2.17%), phosphorus (0.97 to 1.15%), and potassium (1.98 to 2.17%). Additionally, biogas slurry contains micronutrients such as Zn (0.023-0.027 ppm), Cu (0.005-0.009 ppm), Fe (0.32-0.38 ppm), and Mn (0.089-0.094 ppm). Statistical analysis using ANOVA and Post Hoc tests indicated that the mean data values among all three replications do not differ significantly. Therefore, it can be concluded that the nutritive value of biogas slurry is sufficient to reduce the reliance on chemical fertilizers in agriculture. It represents an optimal long-term organic remedy for developing fertile soil, ensuring enduring agricultural productivity, and mitigating the negative environmental impacts associated with waste management.

Behavior of boron and nitrogen impurities in diamonds synthesized at high pressure and high temperature

The effect of nitrogen on the growth of boron-doped diamonds was investigated by removing or adding nitrogen impurities. Optical microscopy images showed that adding a small amount of boron to nitrogen-free diamond completely transformed the diamond into an opaque black color. In the presence of small amounts of boron, the addition of nitrogen diminished the chromogenic properties of boron impurities in diamond. The FTIR spectra showed a compensatory interaction between boron and nitrogen in diamond, causing a portion of the nitrogen to exist as N+ center. Raman spectroscopy confirmed that adding small amounts of nitrogen to diamond reduced the stresses in the diamond and improved its quality, whereas adding excessive amounts of nitrogen reduced the quality. The Hall effect measurements showed that adding nitrogen to boron-doped diamond reduced its p-conductivity, causing an increase in its resistivity and a decrease in its carrier concentration.

Are1-mediated nitrogen metabolism is associated with iron regulation in the mycoparasite Trichoderma atroviride

Trichoderma atroviride is a mycoparasitic fungus with antagonistic activity against fungal pathogens and is used as a pathogen control agent alternative to synthetic fungicides. Sensing nutrient availability in the environment and adjusting metabolism for optimal growth, development and reproduction is essential for adaptability and is relevant to its mycoparasitic activity. During mycoparasitism, secondary metabolites are produced to weaken the fungal prey and support the attack. Are1-like proteins act as major GATA-type transcription factors in the activation of genes subject to nitrogen catabolite repression. Since the quality and quantity of nitrogen has been proven particularly relevant in remodeling the biosynthesis of secondary metabolites in fungi, we decided to functionally characterize Are1, the ortholog of Aspergillus nidulans AreA, in T. atroviride. We show that the growth of the T. atroviride ∆are1 mutant is impaired in comparison to the wild type on several nitrogen sources. Deletion of are1 enhanced sensitivity to oxidative and cell-wall stressors and altered the mycoparasitic activity. We were able to identify for the first time a link between Are1 and iron homeostasis via a regulatory mechanism that does not appear to be strictly linked to the nitrogen source, but rather to an independent role of the transcription factor.

Silicon balance in an integrated multi-tropical aquaculture ecosystem, Sanggou Bay, China

Farmed aquaculture species play an important role in regulating nutrient cycles in farming systems. Compared with nitrogen and phosphorus, the role of farmed species in the silicon (Si) cycle remains poorly understood. To help reduce this uncertainty, we clarified the sources and sinks of silicate and quantified the Si pools in an aquaculture system in Sanggou Bay (SGB). The results showed that dissolved inorganic nutrient levels were significantly lower during the dry season than during the wet. Dissolved silicate (DSi) is a potential limiting factor for phytoplankton growth during spring, and phosphorus limitation occurs during summer. The budget results indicated that large amounts of nitrogen, phosphate (DIP), and DSi were buried in the sediment or transformed into other forms during both the wet and dry seasons. The nitrogen and DIP cycles were strongly influenced by bivalve excretion and farmed species harvesting; however, these processes had little impact on the Si cycle. Si availability depends on both external inputs and internal recycling. DSi was primarily supplied from the Yellow Sea, with a minor contribution from the river due to river discharge during spring. However, during summer, riverine inflow (accounting for 83% of the total influx) was the major DSi source followed by benthic flux (12%). Biogenic silica (BSi) burial efficiency in the sediment was estimated to be 78% during spring and 23% during summer. The BSi preservation efficiency in bivalves during spring was high (53%), leading to a higher Si retention than in river discharge. Bivalves biodeposition plays an important role in the Si burial process. We suggest that this high retention is essentially controlled by the biodeposition mechanism, which is directly controlled by the exotic suspension feeders. Bivalves have the potential to alter Si retention in the bay by producing large amounts of biodeposits and accelerating the silica cycle, which may lead to more carbon dioxide being absorbed by diatoms.

Nitrogen reducing mechanism by microporous aeration based on microbial population characteristics: water temperature factor

ABSTRACT The formation of black odour water is primarily attributed to the elevated concentration of organic pollutants, along with an excessive amount of nitrogen and phosphorus, ultimately leading to an anoxic aquatic environment. The water temperature influence mechanism on black-odorous water restoration by microporous aeration is still lacking depth study. This paper selected (15–18) ℃ (spring and autumn), (22–25) ℃ (summer), (8–11) ℃ (winter) as temperature conditions, and investigated temperature influence on nitrogen reduction. Researches showed that: (1) The removal rates of COD, NH4 +-N and TN were significantly positively correlated with temperature (r = 0.99, 0.96, 0.97), the lowest removal rates were 83.16%, 95.68%, 58.7% ((8–11) ℃), the highest values were 92.67%, 98.27%, 70.96% ((22–25) ℃), respectively. (2) At a temperature range of 22–25°C, the microbial community exhibited the highest levels of abundance, diversity, and uniformity. Notably, Proteobacteria dominated this temperature range with a relative abundance of 79.72%. Furthermore, temperature positively correlated with the majority of dominant bacterial species, suggesting that conditions at 22–25°C are highly conducive to the growth of most bacterial communities. Among these, Limnohabitans, Alsobacter, and Candidatus_Aquirestis, which possess key functions in denitrification and nitrogen removal, displayed significantly higher abundances. It explains the positive correlation between temperature and removal rates of COD, TN and NH4 +-N from microbial population’s perspective. Thus, the best temperature for repairing black-smelly water is (22–25) ℃. This study provides technical reference for mechanism research and practical application of microporous aeration.