Low alloy transformation-induced plasticity aided (TRIP) steels have attracted much interest over the last years. TRIP steels were initially developed for automotive applications as they offer an excellent combination of strength and ductility at reasonable costs. These excellent mechanical properties mainly arise from a complex multiphase microstructure of a ferrite matrix and a dispersion of multiphase grains of bainite, martensite and metastable retained austenite. The relevant influence of microstructure on physical and mechanical properties makes metallographic study essential for an appropriate understanding and improvement of the mechanical behavior. An accurate microstructural characterization and quantification of the amount of the different constituents is indispensable to know how the stresses and strains are distributed within the different microstructural constituents. Among the different characterization methods commonly used electron backscatter diffraction (EBSD) appears to be the unique technique able to observe retained austenite grains often no larger than 1 μm. The present work shows the evolution of retained austenite while straining. Microstructural and textural evolution after different strains was examined by optical microscopy OM, EBSD and XRD techniques on TRIP800 steel. EBSD technique appears as a powerful tool for characterizing the complex multiphase steel microstructure and provides an accurate evaluation of the local crystallographic texture. It allows to measure orientation gradients within individual grains of each different phase. The distinction between some phases is observed.