Quality Traits Research Articles

Chicken production systems, flock composition and structure, genotype and the effect of production systems on egg quality traits in Bovans Brown layers: a case of Bacho and Dawo districts, Ethiopia

Chicken production systems, flock composition and structure, genotype and the effect of production systems on egg quality traits in Bovans Brown layers: a case of Bacho and Dawo districts, Ethiopia

Thiourea improves yield and quality traits of Brassica napus L. by upregulating the antioxidant defense system under high temperature stress

High temperature stress influences plant growth, seed yield, and fatty acid contents by causing oxidative damage. This study investigated the potential of thiourea (TU) to mitigate oxidative stress and restoring seed oil content and quality in canola. The study thoroughly examined three main factors: (i) growth conditions—control and high temperature stress (35 °C); (ii) TU supplementation (1000 mg/L)—including variations like having no TU, water application at the seedling stage, TU application at seedling stage (BBCH Scale-39), water spray at anthesis stage, and TU application at anthesis stage (BBCH Scale-60); (iii) and two canola genotypes, 45S42 and Hiola-401, were studied separately. High temperature stress reduced growth and tissue water content, as plant height and relative water contents were decreased by 26 and 36% in 45S42 and 27 and 42% Hiola-401, respectively, resulting in a substantial decrease in seed yield per plant by 36 and 38% in 45S42 and Hiola-401. Seed oil content and quality parameters were also negatively affected by high temperature stress as seed oil content was reduced by 32 and 35% in 45S42 and Hiola-401. High-temperature stress increased the plant stress indicators like malondialdehyde, H2O2 content, and electrolyte leakage; these indicators were increased in both canola genotypes as compared to control. Interestingly, TU supplementation restored plant performance, enhancing height, relative water content, foliar chlorophyll (SPAD value), and seed yield per plant by 21, 15, 30, and 28% in 45S42; 19, 13, 26, and 21% in Hiola-401, respectively, under high temperature stress as compared to control. In addition, seed quality, seed oil content, linoleic acid, and linolenic acid were improved by 16, 14, and 22% in 45S42, and 16, 11, and 23% in Hiola-401, as compared to control. The most significant improvements in canola seed yield per plant were observed when TU was applied at the anthesis stage. Additionally, the research highlighted that canola genotype 45S42 responded better to TU applications and exhibited greater resilience against high temperature stress compared to genotype Hiola-401. This interesting study revealed that TU supplementation, particularly at the anthesis stage, improved high temperature stress tolerance, seed oil content, and fatty acid profile in two canola genotypes.

Biplot analysis for yield and quality traits and comprehensive evaluation of sugar beet varieties treated by abscisic acid under water deficit stress

Biplot analysis for yield and quality traits and comprehensive evaluation of sugar beet varieties treated by abscisic acid under water deficit stress

OsEXPA7 Encoding an Expansin Affects Grain Size and Quality Traits in Rice (Oryza sativa L.)

BackgroundYield and quality are the two most important traits in crop breeding. Exploring the regulatory mechanisms that affect both yield and quality traits is of great significance for understanding the molecular genetic networks controlling these key crop attributes. Expansins are cell wall loosening proteins that play important roles in regulating rice grain size.ResultsWe investigated the effect of OsEXPA7, encoding an expansin, on rice grain size and quality. OsEXPA7 overexpression resulted in increased plant height, panicle length, grain length, and thousand-grain weight in rice. OsEXPA7 overexpression also affected gel consistency and amylose content in rice grains, thus affecting rice quality. Subcellular localization and tissue expression analyses showed that OsEXPA7 is localized on the cell wall and is highly expressed in the panicle. Hormone treatment experiments revealed that OsEXPA7 expression mainly responds to methyl jasmonate, brassinolide, and gibberellin. Transcriptome analysis and RT-qPCR experiments showed that overexpression of OsEXPA7 affects the expression of OsJAZs in the jasmonic acid pathway and BZR1 and GE in the brassinosteroid pathway. In addition, OsEXPA7 regulates the expression of key quantitative trait loci related to yield traits, as well as regulates the expression levels of BIP1 and bZIP50 involved in the seed storage protein biosynthesis pathway.ConclusionsThese results reveal that OsEXPA7 positively regulates rice yield traits and negatively regulates grain quality traits by involving plant hormone pathways and other trait-related pathway genes. These findings increase our understanding of the potential mechanism of expansins in regulating rice yield and quality traits and will be useful for breeding high-yielding and high-quality rice cultivars.

The Carthamus tinctorius L. genome sequence provides insights into synthesis of unsaturated fatty acids

Domesticated safflower (Carthamus tinctorius L.) is a widely cultivated edible oil crop. However, despite its economic importance, the genetic basis underlying key traits such as oil content, resistance to biotic and abiotic stresses, and flowering time remains poorly understood. Here, we present the genome assembly for C. tinctorius variety Jihong01, which was obtained by integrating Oxford Nanopore Technologies (ONT) and BGI-SEQ500 sequencing results. The assembled genome was 1,061.1 Mb, and consisted of 32,379 protein-coding genes, 97.71% of which were functionally annotated. Safflower had a recent whole genome duplication (WGD) event in evolution history and diverged from sunflower approximately 37.3 million years ago. Through comparative genomic analysis at five seed development stages, we unveiled the pivotal roles of fatty acid desaturase 2 (FAD2) and fatty acid desaturase 6 (FAD6) in linoleic acid (LA) biosynthesis. Similarly, the differential gene expression analysis further reinforced the significance of these genes in regulating LA accumulation. Moreover, our investigation of seed fatty acid composition at different seed developmental stages unveiled the crucial roles of FAD2 and FAD6 in LA biosynthesis. These findings offer important insights into enhancing breeding programs for the improvement of quality traits and provide reference resource for further research on the natural properties of safflower.

Insights into genetic diversity and functional significance of the bHLH genes in cotton fiber development

This study investigated the identification and distribution of single nucleotide polymorphisms (SNPs) within basic Helix-Loop-Helix (bHLH) transcription factors (TFs) across diverse cotton (Gossypium hirsutum) accessions, including wild, landrace, and improved varieties. Comprehensive genomic analyses revealed substantial SNP diversity in wild cotton, distinct from that observed in landraces and improved accessions. Comparative analysis showed notable variations in SNP abundance and impact levels across different accessions, with missense SNPs being prevalent. Nucleotide substitution patterns highlighted the dominance of G>A and A>G substitutions. Population genetic analyses unveiled significant genetic diversity within wild accessions and distinct clustering between wild and improved accessions. Temporal expression profiling of bHLH genes during cotton fiber development demonstrated dynamic expression patterns, emphasizing their roles in fiber initiation, elongation, and cellulose biosynthesis. Moreover, association analysis identified SNPs significantly associated within bHLH genes, particularly GhbHLH149, with fiber quality traits, indicating their potential functional significance. Population genetic analyses further revealed evidence of positive selection on GhbHLH149 during cotton improvement. This study provides a comprehensive understanding of SNP diversity, gene expression dynamics, and genetic selection in bHLH genes, offering valuable insights for future cotton breeding efforts aimed at improving fiber quality traits in cotton.

Bulked Segregant Analysis by Sequencing-Based Genetic Mapping of the Green Spotted Fruit Rind Regulating Locus in Wild Melon XNM020 Reveals Four Possible Candidate Genes

Fruit rind patterns are vital commercial quality traits in melon, in which the spotted or striped fruit rinds contribute to the commercial value of melon and can directly affect the choice of consumers. Although the spotted or non-spotted fruit rind pattern was studied in several cultivated melon accessions, the inheritance and regulating locus of this trait in wild melons are still unknown. Therefore, in this study, the inheritance and regulating loci of the green spotted fruit rind in a wild melon accession XNM020 were explored with F2 segregating populations derived from crossing between XNM020 and a cultivated melon XNM125. Segregating ratios of phenotypic data indicated that the green spotted fruit rind in XNM020 has a monogenic dominant inheritance. BSA-Seq showed that two potential genomic regions on chromosomes 4 (from 0.00 to 2.97 Mb) and 5 (from 0.00 to 2.34 Mb) regulate the formation of the green spotted fruit rind in wild melon XNM020. According to the annotations of polymorphic SNPs (single-nucleotide polymorphisms) and small InDels (insertions and deletions) in target genomic regions and the predicted gene functions, four genes MELO3C003316, MELO3C003375, MELO3C003388, and MELO3C014660 regulating chloroplast development or chlorophyll biosynthesis may be the best candidate genes. The results of this study enriched the inheritances of spotted fruit rinds in melon and also provided target genomic regions for marker-assisted selection breeding of melon focusing on fruit rinds.

Investigation of salt tolerance in cotton germplasm by analyzing agro-physiological traits and ERF genes expression

The development of genotypes that can tolerate high levels of salt is crucial for the efficient use of salt-affected land and for enhancing crop productivity worldwide. Therefore, incorporating salinity tolerance is a critical trait that crops must possess. Salt resistance is a complex character, controlled by multiple genes both physiologically and genetically. To examine the genetic foundation of salt tolerance, we assessed 16 F1 hybrids and their eight parental lines under normal and salt stress (15 dS/m) conditions. Under salt stress conditions significant reduction was observed for plant height (PH), bolls/plant (NBP), boll weight (BW), seed cotton yield (SCY), lint% (LP), fiber length (FL), fiber strength (FS), potassium to sodium ratio (K+/Na+), potassium contents (K+), total soluble proteins (TSP), carotenoids (Car) and chlorophyll contents. Furthermore, the mean values for hydrogen peroxide (H2O2), sodium contents (Na+), catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and fiber fineness (FF) were increased under salt stress. Moderate to high heritability and genetic advancement was observed for NBP, BW, LP, SCY, K+/Na+, SOD, CAT, POD, Car, TSP, FL, and FS. Mean performance and multivariate analysis of 24 cotton genotypes based on various agro-physiological and biochemical parameters suggested that the genotypes FBS-Falcon, Barani-333, JSQ-White Hold, Ghauri, along with crosses FBS-FALCON × JSQ-White Hold, FBG-222 × FBG-333, FBG-222 × Barani-222, and Barani-333 × FBG-333 achieved the maximum values for K+/Na+, K+, TSP, POD, Chlb, CAT, Car, LP, FS, FL, PH, NBP, BW, and SCY under salt stress and declared as salt resistant genotypes. The above-mentioned genotypes also showed relatively higher expression levels of Ghi-ERF-2D.6 and Ghi-ERF-7A.6 at 15 dS/m and proved the role of these ERF genes in salt tolerance in cotton. These findings suggest that these genotypes have the potential for the development of salt-tolerant cotton varieties with desirable fiber quality traits.

The predicted benefits of genomic selection on pig breeding objectives.

The premise was tested that the additional genetic gain was achieved in the overall breeding objective in a pig breeding program using genomic selection (GS) compared to a conventional breeding program, however, some traits achieved larger gain than other traits. GS scenarios based on different reference population sizes were evaluated. The scenarios were compared using a deterministic simulation model to predict genetic gain in scenarios with and without using genomic information as an additional information source. All scenarios were compared based on selection accuracy and predicted genetic gain per round of selection for objective traits in both sire and dam lines. The results showed that GS scenarios increased overall response in the breeding objectives by 9% to 56% and 3.5% to 27% in the dam and sire lines, respectively. The difference in response resulted from differences in the size of the reference population. Although all traits achieved higher selection accuracy in GS, traits with limited phenotypic information at the time of selection or with low heritability, such as sow longevity, number of piglets born alive, pre- and post-weaning survival, as well as meat and carcass quality traits achieved the largest additional response. This additional response came at the expense of smaller responses for traits that are easy to measure, such as back fat and average daily gain in GS compared to the conventional breeding program. Sow longevity and drip loss percentage did not change in a favourable direction in GS with a reference population of 500 pigs. With a reference population of 1000 pigs or onwards, sow longevity and drip loss percentage began to change in a favourable direction. Despite the smaller responses for average daily gain and back fat thickness in GS, the overall breeding objective achieved additional gain in GS.

Genome-Wide Detection of Copy Number Variations and Their Potential Association with Carcass and Meat Quality Traits in Pingliang Red Cattle

Genome-Wide Detection of Copy Number Variations and Their Potential Association with Carcass and Meat Quality Traits in Pingliang Red Cattle

Polymorphisms of ITGA9 Gene and Their Correlation with Milk Quality Traits in Yak (Bos grunniens)

Polymorphisms of ITGA9 Gene and Their Correlation with Milk Quality Traits in Yak (Bos grunniens)

Influence of Zinc and Humic Acid Application on Growth Parameter, Yield and Yield Components and Quality Traits of Black Gram (Vigna mungo L.)

A field experiment was conducted in kharif season during 2021-22 on the field at village Ap-Sonalagi Tq-Jath Dist- Sangli of Maharashtra through Department of Soil Science and Agricultural Chemistry College of Agriculture, Badnapur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, with objectives, to study the influence of zinc and humic acid application on growth parameter, yield and yield components and quality traits of black gram (Vigna mungo L.) The field trial was conducted in Randomized Block Design with five treatments (T1): RDF (25:50:00 N, P2O5, K2O kg ha-1), (T2): RDF+ ZnSO4 @ 25 kg ha-¹, (T3): RDF+ ZnSO4 @ 25 kg ha-¹ + Humic Acid @ 5 kg ha-¹, (T4): RDF+ ZnSO4 @ 25 kg ha-¹ + Humic Acid @ 10 kg ha-¹, (T5) RDF+ ZnSO4 @ 25 kg ha-¹ + Humic Acid @ 15 kg ha-¹ with four replications.According to the results obtained by the field investigation clearly indicated that the significantly higher plant height (44.50 cm), maximum number of branches plant-1 (9.10), number of pods plant-1 (26.15) and grain and straw yield (1305.00 and 2190.00 kg ha-1) at harvest stage were observed in treatment (T5) RDF+ ZnSO4 @ 25 kg ha-¹ + Humic Acid @ 15 kg ha-¹. In grain quality the results revealed that higher protein content (22.15%) and test weight (43.05 g) was also found in the same treatment i.e., (T5) RDF+ ZnSO4 @ 25 kg ha-¹ + Humic Acid @ 15 kg ha- ¹ (22.15%).

From Salinity to Nutrient-Rich Vegetables: Strategies for Quality Enhancement in Protected Cultivation

Salinity, a significant abiotic stressor, imperils vegetable growth, yield, and quality. Moreover, elevated salinity levels, driven by climate change, jeopardize vegetable nutritional quality. In particular, protected cultivation systems, responsible for 60% of the global vegetable industry’s economic value, encounter notable challenges in managing salinity due to water runoff and drainage mechanism limitations. Therefore, it is crucial to understand the intricate mechanisms that control salinity and use this knowledge to improve plant tolerance to these conditions. In this study, we explore strategies to effectively mitigate the detrimental impacts of salinity on vegetable crops cultivated within protected environments. Additionally, we investigate the benefits of controlled moderate salinity adjustments in protected cultivation to enhance their nutritional content. Moderate salinity or nutrient solution increases typically raise total soluble solids, sugar, vitamin C, phenols, lycopene, and antioxidants in most fruit vegetables. Though generally applicable to leafy vegetables, exceptions like lettuce and wild rocket may show inconsistencies, potentially reducing some quality traits. Interdisciplinary approaches are essential to developing sustainable solutions for managing salinity in protected cultivation systems, thereby ensuring the resilience of vegetable production in the face of changing environmental conditions. Given the impracticality of desalinating all areas, future research should also investigate synergies between moderate salinity stress, cultivars used, and environmental factors from physiological and molecular perspectives to enhance vegetable nutritional quality.

Comparative analysis of key fiber characteristics in white Pima cotton (Gossypium barbadense L.): Native accessions from the Peruvian Amazon

AbstractThe fiber quality of cotton (Gossypium barbadense L.) native to the Peruvian Amazon region is one of the most versatile and essential natural fibers in the Peruvian textile industry. There is little information about the fiber quality traits of cotton genotypes native to the Peruvian Amazon region. This study investigated the fiber quality traits of Peruvian Pima cotton accessions native to the Amazon region of the La Convención Province, Cusco, Peru, to determine the lines with the greatest potential for improving fiber quality in cotton genetic breeding programs. A total of 14 cotton accessions with white fiber color, being 12 accessions of G. barbadense L. (Pima cotton) and two accessions of Gossypium sp. (unknown cotton), were analyzed. The fiber properties determined using the high volume instrument method included seven characteristics. All fiber properties were classified into five quality classes. The data on fiber quality properties were subjected to distribution, correlation, and canonical variable analysis. The results of fiber quality properties showed that the two accessions of Gossypium sp. (unknown cotton) can be promising options to be used as high‐quality fiber progenies in crop genetic breeding programs or can be cultivated by regional farmers for the purpose of producing cotton with high fiber quality. Future investigations could utilize other methods of fiber quality analysis and compare the fiber quality of these cotton accessions native to the Peruvian Amazon region with other cotton species grown worldwide to improve the understanding of Pima cotton fiber quality and its applicability in different contexts of the textile industries.

Detection and Analysis of Quality Traits of Different Black Rice Varieties in the Southern Henan Rice Region

Using 14 black rice varieties imported from Southern Henan as experimental materials, the appearance quality, grinding quality, nutritional quality, cooking and eating quality of grain in Southern Henan were tested and analyzed by means of rice huller, rice mopping machine and near-infrared grain analyzer, combined with biochemical and physical analysis techniques. The results showed that there were abundant variation types in grain length, grain width, length-to-width ratio, brown rice rate, milled rice rate, head rice rate, soluble protein content, starch content, gelatinization temperature, gel consistency and viscosity of black rice. Particularly, grain type, starch content, gelatinization temperature, viscosity and other important quality traits in black rice materials have a large variation range. Therefore, our results provide important clues for developing new high-quality varieties of black rice.

A genome-wide association study uncovers a ZmRap2.7-ZCN9/ZCN10 module to regulate ABA signalling and seed vigour in maize.

Seed vigour, including rapid, uniform germination and robust seedling establishment under various field conditions, is becoming an increasingly essential agronomic trait for achieving high yield in crops. However, little is known about this important seed quality trait. In this study, we performed a genome-wide association study to identify a key transcription factor ZmRap2.7, which regulates seed vigour through transcriptionally repressing expressions of three ABA signalling genes ZmPYL3, ZmPP2C and ZmABI5 and two phosphatidylethanolamine-binding genes ZCN9 and ZCN10. In addition, ZCN9 and ZCN10 proteins could interact with ZmPYL3, ZmPP2C and ZmABI5 proteins, and loss-of-function of ZmRap2.7 and overexpression of ZCN9 and ZCN10 reduced ABA sensitivity and seed vigour, suggesting a complex regulatory network for regulation of ABA signalling mediated seed vigour. Finally, we showed that four SNPs in ZmRap2.7 coding region influenced its transcriptionally binding activity to the downstream gene promoters. Together with previously identified functional variants within and surrounding ZmRap2.7, we concluded that the distinct allelic variations of ZmRap2.7 were obtained independently during maize domestication and improvement, and responded separately for the diversities of seed vigour, flowering time and brace root development. These results provide novel genes, a new regulatory network and an evolutional mechanism for understanding the molecular mechanism of seed vigour.

GENETIC STUDIES FOR SOME YIELD-RELATED TRAITS IN OKRA (ABELMOSCHUS ESCULENTUS L.)

Okra is a traditional vegetable famous for its nutritional value. It is a perfect villager's vegetable due to its robust nature, dietary fiber, and seed protein balance of both tryptophan and lysine. The research was carried out in the research area of the Department of Plant Breeding and Genetics, University of Agriculture Faisalabad. Cross combinations among 3 lines (Patel, Selection Super Green, and 19234) and 3 testers (IQRA-III, Perbhani Karanti, and Sabz Pari) were developed by using line × tester design. Crossed seeds along with parents were grown in the field under Randomized Complete Block Design (RCBD) with three replications. Data for various morphological traits was collected and analyzed statistically through analysis of variance and used to assess GCA, SCA, and gene action. Genotype 19234 exhibited maximum significant GCA (-11.4) for plant height in the negative direction and genotype Selection super Green proved a good general combiner for fruit weight, fruit diameter, and first flowering node, exhibiting values of 3.21, 1.191, and -0.89. IQRA-III (3.00), Patel (2.00), and Perbhani Karanti (1.11) exhibited significant GCA for number of fruits per plant. Line 19234 observed a good general combiner for crude fat (0.28), ash (5.43), and protein (0.39). Cross combination Patel × Perbhani exhibited the highest SCA for the number of fruits (7.78) followed by Selection Super Green × Sabz Pari (4.78) hence can be exploited for heterosis breeding. Cross combination Selection Super Green × Perbhani Karanti exhibited the highest significant SCA for plant height (-7.60) and Selection Super Green × IQRA-III displayed the maximum significant SCA value (2.26) for crude fat. Line Patel proved a good general combiner for most of the yield-related traits. Based on GCA and SCA, line 19234 can be used to enhance quality traits and cross combination Patel × Perbhani Karanti can be used to increase the number of fruits per plant.

Effect of Planting Density and Nitrogen Levels on the Yield Attributing Characters of Fodder Maize (Shalimar Fodder Maize-1)

Maize (Zea mays L.) is widely known as a ready-made fodder crop. Maize is the third major cereal crop in the world, and in India, it ranks third. Maize has been an important cereal crop owing to its highest production potential and wider adaptability to varied agroclimatic conditions, hence being called the ‘Queen of Cereals’. The production potential of forage maize can be altered with changes in agronomic practices. The present study was undertaken with the objective of improving the yield attributes and quality traits of forage maize (Shalimar Fodder Maize-1). The experiment consisted of two factors, viz., three planting densities (viz., D1= 30cm x 10cm, D2= 40cm x 10cm, and D3= 50cm x 10cm) as main plots and five nitrogen levels (viz., N1= Control, N2= 75% RDN (recommended dose of nitrogen), N3= 100% RDN, N4= 125% RDN, and N5= 150% RDN) as subplot treatments with three replications, laid out in split plot design (SPD). The parameters such as yield attributes, and quality parameters were significantly higher when plants were grown at a density of 30 cm x 10 cm with a nitrogen level of 150 % RDN.

Early impact assessment of the paninvasive polyphagous shot hole borer beetle on commercial pear production

AbstractThe polyphagous shot hole borer (PSHB) beetle, Euwallacea fornicatus (Eichhoff) (Coleoptera: Curculionidae, Scolytinae), and its phytopathogenic fungal symbiont (Fusarium euwallaceae Freeman et al.) threaten commercial and urban tree species worldwide. This pest–fungus complex has been newly discovered in the commercial deciduous fruit industry (Western Cape, South Africa), where its effect is still unknown. This study explored the impact of PSHB infestation on fruit quality and plant water‐use efficiency (WUE) – as indicated by carbon and nitrogen stable isotope ratios – of commercially cultivated Packham's Triumph pear trees within the first year of invasion, as well as changes in phenotypic plasticity (trait expression range). The impact of PSHB infestation is currently most evident in fruit quality traits, with a clear distinction between the control and most densely attacked tree categories. Fruit and leaf traits showed high variation and a significant change in phenotypic plasticity. Increased PSHB attack density was associated with smaller, duller‐toned, sweeter fruit at the time of harvest and data tended towards reduced plant WUE. Producers can expect significant variation in fruit quality and plant WUE even in hosts without branch dieback, visible plant stress or extensive beetle breeding, especially in orchards where infestations persist beyond the first year. These findings necessitate additional research to describe the long‐term impact, examine the threat to other deciduous fruit crops and cultivars and assess the need for adapted management or harvesting methods.

Assessing genetic diversity of tomato (Solanum lycopersicum) genotypes cultivated under protected condition

Present study was carried out during 2018–19 and 2019–20 at ICAR-Indian Agricultural Research Institute, New Delhi to evaluate 16 quantitative and qualitative traits of 36 genotypes of Tomato (Solanum lycopersicum L.) and to examine the genetic variability and character association between yield and its component traits. This investigation involved correlation and genetic divergence analyses to gain insights into the relationships among the various traits. The variance analysis revealed notable variations among genotypes, indicating a substantial level of genetic diversity among them. Among the genotypes, 178 and 115 were found promising for both yield and quality traits. Genotype no. 178 was found superior for fruit weight and yield. The principal component analysis indicated that the first 3 components i.e. component 1 (equatorial diameter, average fruit weight and fruit weight uniformity); component 2 (number of locule and equatorial/polar index); and component 3 (number of fruit and number of fruit/cluster) collectively accounted for 81.48% of the variability. The yield exhibited significant positive correlation with average fruit weight (g), pericarp thickness (cm), polar diameter of fruit (cm), equatorial diameter of fruits (cm) and average fruit weight after each harvest (kg).