Introduction: Microbiological pollutants in indoor air can lead to sick building syndrome (SBS). A conducive academic environment in university dormitories requires healthy indoor air quality to enhance student performance. This study aimed to determine the association between indoor air microbiological exposure and SBS among college dormitory students at a public university. Methods: A cross-sectional study was conducted among 112 male and female college dormitory students who stayed in the public university dormitory for more than three months. Respondents were given a self-administered questionnaire-based NIOSH Indoor Environmental Quality Survey. 14 houses were selected for the measurement of indoor air quality which included PM2.5, PM10, relative humidity (%), temperature (°C), air velocity (m/s), indoor bacterial concentration (cfu/m3), and indoor mould concentration (cfu/m3). Results: Higher percentages of SBS symptoms were reported among female (94.6%) college dormitory students compared to male (82.1%). A significant difference in itching symptom on the arms and forearms was found among individuals of different genders (p<0.05). Indoor air parameters such as temperature (OR = 2.56, 95% CI = 2.66-9.87), relative humidity (OR = 1.77, 95% CI = 1.54 – 5.79), indoor bacteria concentration (OR=2.28, 95% CI = 2.48 – 10.93), and indoor mould concentration (OR=2.59, 95% CI = 1.75 – 8.98) were significantly associated with the recent SBS symptoms. Multiple logistic regression showed that the most significant predictor of SBS was indoor mould concentration. Conclusion: Exposure to high concentrations of indoor bacteria and mould, high temperatures, and high relative humidity influenced the prevalence of SBS among college dormitory students. Further assessment and preventive action need to be taken to reduce the exposure of indoor air microbiological to the students.