A health care system is intertwined with multiple stakeholders, including government institutions, pharmaceutical companies, patients, hospitals and clinics, health care professionals, health researchers and scientific medical experts, patients and consumer organizations, and media organizations. Physicians and journalists are the key actors who play a significant role in making health care services and health information accessible to the people of a country. The aim of this study was to explore the tensions and alliances between physicians and journalists in Bangladesh, along with identifying strategies that could potentially improve the often contentious relationship and quality of medical journalism. We conducted a web-based cross-sectional survey using the snowball sampling technique from September 2021 to March 2022. Adult Bangladeshi citizens belonging to the two selected professional groups (physicians and journalists), who understood the survey content, and agreed to participate in the survey were considered eligible for inclusion in the study. Both descriptive and logistic regression analyses, including the Mann-Whitney U test and Wilcoxon signed-rank test, were performed to determine the differences between groups concerning selected perception-related variables, and the associations of perceptions about lack of trust in each other's knowledge, skills, and professional integrity with background characteristics. A total of 419 participants completed the survey, including 219 physicians and 200 journalists. Among physicians, 53.4% (117/219) reported lower trust toward journalists' professional domain and expertise, whereas 43.5% (87/200) of journalists had lower trust toward physicians' professional domain and expertise. In terms of perception about not having respect for each other, the median value for the physicians was 5 (strongly agree), whereas it was only 3 (agree) for the journalists. We also found that male physicians (adjusted odds ratio [AOR] 0.45, compared with female physicians) and medical officers (AOR 0.30, compared with specialists) had significantly higher odds of lacking trust in journalists' knowledge, skills, and professional integrity. When rating the statement "Regular professional interaction between journalists and doctors may improve the relationship between the professional groups," most physicians (186/219, 84.9%) chose "neither agree nor disagree," whereas most journalists (106/200, 53.0%) stated that they "slightly agree." Both physicians and journalists in Bangladesh have negative perceptions of each other's professions. However, physicians have a more negative perception of journalists than journalists have of the physicians. Strategies such as a legal framework to identify medical-legal issues in reporting, constructive discussion, professional interaction, and capacity-building training programs may significantly improve the relationship between physicians and journalists.