This paper integrates major, minor, trace and rare-earth element (REE) data into C-, O-, Sr-isotopes, fluid-inclusions microthermometry of replacive matrix dolomite and vug/fracture-filling saddle dolomite and calcite of the Lower Cretaceous Qamchuqe Formation, the Kurditan Region of Iraq, in order to get further insights into the origin and geochemical evolution of their parent diagenetic fluids. Multiple generations of dolomite have been observed. These dolomites constitute replacive matrix dolomite and saddle dolomite cement, dominantly poorly ordered, non-ferroan, non-stoichiometric, with an average of 59.4 and 60 mol% CaCO3, respectively, and have low Sr, Fe and Mn concentrations. Replacive matrix dolomites display fairly comparable REE patterns to saddle dolomite, but with light REE (LREE) enrichment, and moderate low (∑REEs) contents (18.9–1111.5 and 16.6–593.4 ppb, respectively). Saddle dolomite has moderately positive Eu anomalies while replacive matrix dolomite has slightly less positive Eu anomalies and negative Dy, Ho and Yb anomalies. Both dolomites are characterized by slightly radiogenic 87Sr/86Sr ratios ranging from 0.707702 to 0.707839, overlapping, wide-ranging and negative δ18O values from −10.43 to −4.47 and −12.87 to −7.79 ‰VPDB, and δ13C values ranging from 0.38 to 3.57 and −3.45 to 3.11‰VPDB, respectively. The primary fluid inclusions microthermometry of replacive matrix and saddle dolomites yield homogenization temperatures (Th) ranging from 71.3 to 228.3 °C and 82–188 °C, respectively, and salinity values ranging between 14.4 to 25.4 and 15.6 to 27.9 wt% NaCl eq., respectively. These data suggest multiple hydrothermal fluid fluxes during and post Zagros orogeny. The geochemical data also show that these fluids derived from similar source of mixed hydrothermal and marine fluids, which caused the dolomitization of host rock and precipitation of dolomite cements.