In order to explore the characteristics of pressure pulsation signals and energy distribution of water flow at the guide vane considering impeller-guide vane interaction. The numerical simulation of the vertical axial flow pump device's steady and unsteady three-dimensional flow fields was carried out. The Hilbert-Huang method was used to conduct empirical mode decomposition decomposition and Hilbert spectrum analysis of pressure pulsation signal at each monitoring point in the inlet and outlet regions of the guide vane. The results show: Under the condition of 0.3Qbep, the internal pressure of the guide vane is obviously affected by the impeller, and there are large block-shaped vortex structures in the guide vane. Under the operating conditions of 1.0Qbep and 1.2Qbep, the size of the pressure area in the guide vane is basically not affected by the impeller, and the vortex structures in the guide vane are concentrated near the outlet of the guide vanes, and there are long strip-shaped vortex structures at the edge of the guide vane. The size and number of vortex structures decrease with the increase in flow rate. The pressure pulsation signal at the inlet of the guide vane is affected by the rotation of the impeller and exhibits good periodicity, with the main frequency centered around 146 Hz, and the energy ratio of the main frequency is up to 97.7%. There are low-frequency signals below 100 Hz and high-frequency signals fluctuating around 146 Hz in all three flow conditions. When the flow rate increases, the fluctuation amplitude of the high-frequency signal increases. The flow rate has a significant impact on the water flow at the outlet of the guide vane. At 0.3Qbep, its frequency is distributed in the range of 0-500 Hz, mainly concentrated in the area below 400 Hz. At 1.0Qbep, the frequency of pressure pulsation is distributed below 250 Hz after the guiding function of the guide vane. At 1.2Qbep, the water flow is mainly controlled by the rotation of the impeller, and after the energy recovery of the guide vane, its main frequency is still concentrated around 150 Hz, which is 337.2% and 268.5% of 0.3Qbep and 1.0Qbep. Under the working condition of 0.3Qbep, the proportion of intrinsic mode function energy corresponding to the dominant frequency at the center of the guide vane inlet is as high as 95.9%, and the proportion of intrinsic mode function energy corresponding to the dominant frequency at the shroud side and hub side of the guide vane is rather low. If the flow rate rises from 0.3Qbep to 1.2Qbep, the proportion of intrinsic mode function energy increases by more than 42%. Under the working conditions of 0.3Qbep and 1.0Qbep, the main frequency of pressure pulsation signal of water flow at the guide vane outlet is less affected by the impeller and the corresponding energy proportion is low. Under the working condition of 1.2Qbep, the main frequency of pressure pulsation signal is 4 times the rotational frequency and the corresponding energy proportion is higher than 60%.