AbstractAnxiety and depression are the two most common mental illnesses in the world. Multiple risk factors contributein developing anxiety and depression; the latter leading to suicide. The aim of this study is to identify theprevalence and associated factors of anxiety and depression, and assess their association with the level ofresilience among academic and non-academic staff of Herat University. This cross-sectional study wasconducted between November 1st and December 28th 2021. Sociodemographic data was collected using astructured questionnaire. Data on depression and anxiety was collected using the validated Dari version ofDASS-42 questionnaire. Data on resilience was obtained using the 10-item Connor-Davidson Resilience Scale(CD-RICS-10). Statistical analyses were performed in IBM SPSS Statistics (version 27). A total of 210university staff including 154 (73.3%) male and 56 (26.7%) female, with a mean age of 37.67 ± 9.62 years wereincluded in the study. Of All participant in this study, 58.6% and 54.8% had anxiety and depression,respectively. There was a significant association between resilience with depression and anxiety. A significantassociation was found between depression, anxiety and resilience with economic status, general healthcondition, concern about food shortage, losing house, and losing job. While no significant association was foundbetween depression, anxiety and resilience with gender, marital status, and losing family member in childhood.The prevalence of mental illnesses is considerably high among Herat University staff. The leadership of HeratUniversity and public health authorities should implement strategies to tackle this important public healthchallenge.