The utility of seed addition to promote tree regeneration can be greatly limited by animals that consume seeds. Moreover, given that restoration often occurs in forests where invasive shrubs are abundant, and evidence that invasive shrubs can increase granivory, it is important to explore whether methods for reducing granivory work equally well in invaded and uninvaded habitats. We used a multi-site field experiment spanning 160 days to explore whether coating seeds of Prunus serotina with capsaicin extract leads to reduced granivory in habitats with or without invasive shrubs (Rhamnus cathartica). Capsaicin-coated seeds were removed at a similar rate to uncoated seeds, but seeds in invaded plots had a 78.8% higher rate of removal compared to plots without invasive shrubs. Our findings suggest that managers seeking to encourage regeneration of native trees using direct seeding should consider invasive shrub removal as a top priority to limit the loss of seeds once sown.