This study aimed to synthesize the biochar of Prosopis juliflora (PJ) and apply it to the adsorption of antibiotic residues sulfamethoxazole (SMZ) and ciprofloxacin (CIX) from pharmaceutical industry wastewater. The PJ biochar was synthesized using the pyrolysis method and characterized by proximate analysis, TGA, SEM-EDX, FTIR, XRD, and BET methods. The moisture content, volatile matter, ash content, and fixed carbon were found to be 6.4 %, 72.3 %, 3.0 %, and 18.3 %, respectively. Moreover, biochar characteristics were described by a high BET surface area of 875 m2/g, various FTIR peaks with different functional groups, high TGA thermal stability at increasing temperature, amorphous XRD structure, and high SEM surface morphology, and major EDX carbon compositions of 97 %. These analysis results demonstrated insights into the potential and nature of biochar adsorbent. The maximum adsorption of SMZ and CIX was found to be 98.7 % and 97.8 %, respectively, at a contact time of 120 min, initial concentration of 50 mg/L, pH (5 for CIX and 8 for SMZ), and adsorbent dose of 1 g/L for a synthetic solution. On the other hand, COD and TOC of real wastewater were reduced from 2498 to 514 mg/L and 1050 to 124 mg/L during the real wastewater treatment. Target pollutants concentration in real wastewater was found to be 8.29 and 5.3 mg/L for CIX and SMZ and their removal efficiency was obtained as 80.4 % and 76.7 % respectively. The obtained experimental data were best fitted with the Langmuir isothermal model at R2 = 0.999, implying the adsorbent surface is homogeneous and monolayer. Similarly, the adsorption kinetics process was well described by a pseudo-second-order kinetics model for both SMZ and CIX at R² = 0.999. Finally, the non-linear intra-particle diffusion graphs suggest that chemical adsorption is the main influencing factor for overall kinetics. In conclusion, using PJ biochar is a promising precursor in advancing adsorbents for removing contaminants from industrial wastewater to address environmental pollution challenges.