Given that agro-industrial activity is widely seen as a means of promoting development and the production of bioenergy has come to be considered a means of both fostering socioeconomic development and reducing greenhouse gas emissions, the production of biodiesel would appear to be a means of promoting development in developing countries. The situation of Guinea-Bissau suggests the country may benefit from a biodiesel production program. Thus, this paper uses Brazil’s experience with biodiesel as a basis for proposing a framework for a Biodiesel Program in Guinea-Bissau. The proposed framework includes the following elements related to public policy, regulations, organizations and mechanisms: (i) introduction of biodiesel through the creation and implementation of laws and regulations; (ii) an Inter-ministerial Executive Committee to plan, coordinate and manage the program; (iii) promotion, incentives and support for agricultural and agro-industrial production by providing tax benefits for specific links of the chain, creating incentive programs for different oilseeds and establishing programs that support family farming; and (iv) the creation and installation of representative bodies for the stakeholders involved in the chain, such as small farmers cooperatives, national association of biodiesel producers, R&D and rural extension programs.