Methods. The theoretical and methodological basis of the research is the scientific works of domestic and foreign scientists on the problems of the knowledge economy and economic culture in the formation of skills of the personnel of the transport industry. The research methodology is based on the system approach, within which the following logical, general scientific, theoretical and empirical methods are applied: content analysis – to clarify a number of concepts that characterize the knowledge economy and economic culture in the formation of personnel skills; abstract-logical method – for theoretical generalizations and formulation of research conclusions. Results. Skill formation is necessary in order to reduce the time it usually takes for staff to gain experience in a new location, to quickly «blend» into the team, understand the demands placed on them, and quickly reach the required level of productivity. For a transport company, the skills formed significantly reduce staff turnover, which leads to a reduction in recruitment costs. The use of elements of economic culture as a system education includes professional knowledge, abilities, skills, special intellectual abilities and qualities, moral principles and value norms of personnel for providing transport services to the population. Novelty. Knowledge, qualifications, skills, abilities, competences, development, continuous training of personnel in the conditions of the knowledge economy are considered as integral components of the effective functioning and innovative development of the transport enterprise. The birth of new spheres of economic activity, the emergence of new knowledge and technologies, increased competition, changed the priorities of the development of most transport enterprises, putting people and their skills in the foreground as the main resource of economic growth. Practical value. The development directions of various tools for the effective formation of personnel skills have been formed, which allows to provide the transport enterprise with highly qualified and competitive personnel, able to quickly cope with the assigned tasks, demonstrate a sufficient level of professional competence and experience, become part of the team and find a common language with the direct manager. The importance of the knowledge economy and economic culture for solving tasks that affect the reduction of personnel management costs, the reduction of personnel turnover, and the improvement of the efficiency of its work are substantiated.