Agrochemicals have been identified as endocrine disruptors affecting many bodily functions of animals, especially the fertility of males. Evidence of various agrochemicals affecting male reproductive functions are plenty. However, evidence on disruptive effects of fungicides on sperm functions is scarce. Therefore, an in vitro study was conducted to investigate acute effects of the fungicide Mancozeb on spermatozoa functions using a bovine spermatozoa model. Semen samples were obtained from fertility proven Jersey bulls and were exposed to series of Mancozeb concentrations (0.01 μg/ml to 10 μg/ml), and the changes occurred in sperm motility parameters, acrosine proteolytic activity and acrosome integrity were assessed at different time intervals. Pure and commercial forms of Mancozeb were in the study, and sperm motility parameters were measured using computer assisted sperm analysis (CASA). Mancozeb concentration of 1 μg/ml with 2 hours exposure significantly (p≤0.05) reduced the total sperm motility and the sperm progressive motility. Velocity parameters like average path, straight line, curvy linear and amplitude lateral head displacement were significantly (p≤0.05) reduced after 2 hours in both pure and commercial Mancozeb treatments of 1 μg/ml. However, the beat cross frequency and elongation of the sperm were significantly (p≤0.05) reduced only with commercial Mancozeb. Further, the acrosome integrity and proteolytic activity of the acrosome enzymes were significantly (p≤0.05) compromised by both Mancozeb treatments. The study revealed that Mancozeb (>1 ug/ml) could reduce the sperm motility and impairment of acrosome activity significantly. Nevertheless, commercial Mancozeb formulation has the highest impact on the sperm functions. Thus, Mancozeb may affect the fertilization capacity of the spermatozoa under acute exposure.