Abstract
This article describes the enzymatic activity of proteolytic enzymes, the general chemical composition of the digestive organs of carnivorous and herbivorous fish in the period from October to November 2017 to April 2018. The dependence of the enzymatic activity on the pH of the medium and the mass fractions of protein and fat is shown. Based on the data obtained, enzymes were isolated from the digestive organs of bream (at pH 9.5) and pike-perch (at pH 2.5). To determine the activity of the test substance, we used the corrected absorbance for the test suspension (the difference in absorbance between the test sample and the control sample) obtained using raw materials for the period January to February 2018. We also carried out the purification of enzyme preparations using ion exchangers (Q-XL, SP-XL) and size exclusion chromatography (Sephadex G-75 Superfine), determining the molecular weight of enzyme preparations prepared from the digestive organs of pike-perch and bream.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.