Wheat Grain Protein Research Articles

Influence of ecological factors on formation of quality of grain of winter wheat (Triticum aestivum L.) of varieties of myronov selection

The research was conducted during 2015–2018 in the conditions of the V.M. Remeslo Myronivka Institute of Wheat National academy of agrarian sciences of Ukraine (Forest-Steppe) and the Nosivska Plant Breeding and Experimental Station (Polissia). The research was carried out in accordance with conventional methods and guidelines. Weather conditions were contrasting, namely optimal in 2015/16, arid in 2016/17 and excessively humid — 2017/18. It was found that they significantly affected protein content. Thus, the largest one was formed in 2016/17, when in the period from the beginning of milk state of grain to the end of wax ripeness the hydrothermal coefficient (HC) was 0.7. Less protein content was formed in 2015/16 and 2017/18, when cool weather with high humidity was observed during grain filling. The protein content of the varieties was in the range of min 8.20 and max 12.95% in the ForestSteppe, min 8.10 and max 11.80% in the Polissia. In 2017/18, in the conditions of Polissia there was observed a phenomenon when gluten content was slightly lower at almost the same protein content in the varieties studied as compared with its content in these varieties in the Forest-Steppe zone. During the period from full ripeness to harvesting varieties in Polissia and Forest-Steppe in 2017/18 HC = 4.2 and 2.5, respectively. Wheat varieties have formed on average for three years gluten content in the range of min 20.18 and max 26.09% in the Forest-Steppe, min 15.50 and max 30.0% in the Polissia. There were found moderate correlation r = 0.56 between grain protein and gluten content for the varieties studied in the Forest-Steppe zone and strong correlation r = 0.85 in the Polissia zone. The ratio of gluten to protein in winter wheat grain on average varied from 1.7 in some points of climatic conditions of the ForestSteppe and Polissia in 2015/16 for the variety MIP Valensiia to 2.7 for the variety Hratsiia myronivska in the Forest-Steppe zone in 2016/17 and for the variety Horlytsia myronivska in the Polissia zone in the same year. The impact of the factor «variety» on protein content was 19%, interaction of factors «variety — year» was 15%, the influence of the factor «year» on gluten content in winter wheat grain was the maximal — 41%, the interaction of factors «variety — year» was 14%. Weather conditions of winter wheat cropping season, especially during grain formation, significantly affected the quality indices. They are formed the best under moderate supply of plants with moisture to and worse — with excessive moisture. Increase in gluten mass fraction significantly depended on the conditions of the year and varietal characteristics

Neural Modeling of the Distribution of Protein, Water and Gluten in Wheat Grains during Storage

An important requirement in the grain industry is to obtain fast information on the quality of purchased and stored grain. Therefore, it is of great importance to search for innovative solutions aimed at the monitoring and fast assessment of quality parameters of stored wheat The results of the evaluation of total protein, water and gluten content by means of near infrared spectrometry are presented in the paper. Multiple linear regression analysis (MLR) and neural modeling were used to analyze the obtained results. The results obtained show no significant changes in total protein (13.13 ± 0.15), water (10.63 ± 0.16) or gluten (30.56 ± 0.54) content during storage. On the basis of the collected data, a model artificial neural network (ANN) MLP 52-6-3 was created, which, with the use of four independent features, allows us to determine changes in the content of water, protein and gluten in stored wheat. The chosen network returned good error values: learning, below 0.001; testing, 0.015; and validation, 0.008. The obtained results and their interpretation are an important element in the warehouse industry. The information obtained in this way about the state of the quality of stored grain will allow for a fast reaction in case of the threat of lowering the quality parameters of the stored grain.

Genetic variation and heritability of grain protein deviation in European wheat genotypes

There is a well-established negative relationship between the yield and the concentration of protein in the mature wheat grain. However, some wheat genotypes consistently deviate from this relationship, a phenomenon known as Grain Protein Deviation (GPD). Positive GPD is therefore of considerable interest in relation to reducing the requirement for nitrogen fertilization for producing wheat for breadmaking. We have carried out two sets of field experiments on multiple sites in South East England. The first set comprised 11 field trials of 6 cultivars grown over three years (2008-2011) and the second comprised 9 field trials of 40 genotypes grown over two years (2015-2017) and 5 field trials of 30 genotypes grown in a single year (2017-2018). All trials comprised three replicate randomized plots of each genotype and nutrient regime. These studies showed strong genetic variation in GPD, which also differed in stability between genotypes, with cultivars bred in the UK generally having higher GPD and higher stability than those bred in other European countries. The heritability of GPD was estimated as 0.44, based on data from the field trials of 30 and 40 genotypes. The largest component contributing to the genetic variance was genotype (0.30), with a smaller contribution of the interaction between genotype and year/site (0.11) and a small (but statistically significant) contribution of nitrogen level. These studies suggest that selection for GPD is a viable target for breeders.

Protein quality of winter wheat depending on the dressing with sulfur-containing nitrogen fertilizers applied in early spring

The paper contains the study results of the effect of sulfurcontaining nitrogen fertilizers on the quality of protein in winter wheat grain grown in the Ulyanovsk region on soils with a lack of sulfur. The experimental crop is soft winter wheat, the variety “Saratovskaya – 17”. As a result, it was found that the biological value of protein compared to the control option increased by 11.5% in the option with the use of 5% of sulfur.

ВЛИЯНИЕ МИНЕРАЛЬНЫХ УДОБРЕНИЙ НА УРОЖАЙНОСТЬ И КАЧЕСТВО СЕЛЬСКОХОЗЯЙСТВЕННЫХ КУЛЬТУР В ДЛИТЕЛЬНОМ СТАЦИОНАРНОМ ОПЫТЕ В КЛИМАТИЧЕСКИХ УСЛОВИЯХ ПРЕДУРАЛЬЯ

A stationary field experiment carried out in 1978 on sod-podzolic heavy loamy soil (Eutric Albic Retisols (Abruptic, Loamic, Cutanic)) found that the application of mineral fertilizers in increasing doses led to acidification of the soil by the end of the fifth crop rotation in the arable layer with pH 5.6 when laying the experiment to pH 4.5 in the (NPK)150 variant. The use of NPK at 90–150 kg ai/ha contributed to the preservation of the initial humus level, an increase in the content of mobile phosphorus to 452 and exchangeable potassium to 403 mg/kg. The yield of cereals, potatoes and meadow clover in the crop rotation depended on hydrothermal conditions during the growing season of plants and the level of mineral nutrition. The yield of winter rye amounted to 4.51–4.85 t/ha and practically did not depend on the dose of fertilizers applied. As an NPK dose increased, the protein content in winter rye grain increased from 8.32% in the control samples to 11.57% in the samples with the maximum dose of the complex fertilizer. Potatoes produced a maximum yield (21.81 t/ha) when fertilized at a dose of 90 kg/ha. At increased doses of NPK in potato tubers, a decrease in starch content and an increase in nitrates above MAC were observed. The best indicators of yield (2.5 t/ha) and spring wheat quality were determined on options (NRK)60 and (NRK)90, the increase in control was 0.76–0.82 t/ha. The highest content of protein (18.41%) and gluten (37.48%) in wheat grain was determined by applying NPK at a rate of 60 kg ai/ha. High weediness of crops and adverse weather conditions did not provide for full implementation of the yield potential of meadow clover. In the conditions of the dry growing season of 2016, the maximum yield of barley was obtained by applying a full mineral fertilizer at a rate of 120–150 kg ai/ha (2.54–2.79 t/ha), the surplus to the option without the use of fertilizers was 0.99–1.24 t/ha (НСР05 = 0.15). The Stayer spring oats produced a maximum yield of 5.43 t/ha with NPK application at a rate of 90 kg ai/ha. The nitrogen content in the grain was very low and did not depend on the application of mineral fertilizers.

Вплив строків сівби на урожайність і якість зерна пшениці озимої

Вплив строків сівби на урожайність і якість зерна пшениці озимої

The overexpression of high-molecular-weight glutenin subunit Bx7 improves the dough rheological properties by altering secondary and micro-structures of wheat gluten.

Bread wheat (Triticum aestivum L.) is one of the crucial cereals consumed by human beings and wheat gluten, the natural macromolecules, mainly determines the processing quality of wheat dough. The high-molecular-weight glutenin subunits (HMW-GSs) of gluten proteins are recognized as one of the main components regulating the rheological properties of dough. The overexpressed Bx7 subunit (Bx7OE) has been reported to improve wheat quality and rheological properties of dough, however its effect on secondary and micro- structures of gluten is still unclear. In this study, we evaluated the composition of main storage proteins in wheat grains of two near-isogenic lines and studied the effect of Bx7 subunit expression level on the secondary structures of gluten and micro-structure of gluten during dough mixing process. Results showed the protein content, HMW-GSs proportion in total glutenins and free sulfhydryl content increased in the flour of HMW-Bx7OE wheat line, and the accumulation of unextractable polymeric protein during grain filling stage accelerated. It was found that the content of β-sheets in secondary structures of gluten increased and a more compact micro-structure of gluten network formed in the dough. Protein network analysis characterized and quantified the alterations in the gluten micro-structure. In the process of dough mixing, protein area, total protein length, number of junctions and branching rate reach the peak at dough development time, which was consistent with Chopin mixing profile. Interestingly, during dough mixing, the above-mentioned parameters of HMW-Bx7OE showed less changes than those of HMW-Bx7 wheat line, indicating Bx7OE improved the dough stability during mixing. To conclude, Bx7OE alters the secondary and micro- structures of gluten and thus improves the mixing and rheological properties of wheat dough.

Influence of Phosphorus Fertilizers Application on Phosphorus use Efficiency and Grain Protein of Winter Wheat in Alkaline-Calcareous Soil, Southern Algeria

hree field experiments (2008 to 2011) were conducted to evaluate the ability of winter wheat (Triticum durum Desf. var. Carioca) to utilize and acquire soil phosphorus and nitrogen under different sources of phosphate fertilizers (Fosfactyl, Single super phosphate and Mono-ammonium-phosphate) and rates of applications (30, 60, 90 and 120 kg Pha-1) with a fixed level of nitrogen fertilization. Results revealed a significant effect of P rates on phosphorus use efficiency (PUE) and nitrogen use efficiency (NUE); however, P sources fertilizer had non-significant effect in all three cropping seasons. PUE and NUE decreased markedly with increasing P supply that did not result in an improvement of P and N uptake. Grain protein content was significantly influenced by phosphorus fertilization in 2008-09. The best values were obtained by Single super phosphate and P rate 60 kg ha-1.

Influences of phosphorus and foliar iron fertilization rate on the quality parameters of whole wheat grain.

The objective of this research was to study the effects of phosphorous and foliar iron fertilizations rates on the of protein, fiber, and water contents in wheat grains. Treatments of fertilizers included a combination of three foliar iron rates (0, 1.2, and 2 L/ha, Fusion) and four phosphorus rates (0, 75, 150, and 225 kg/ha, P2O5) at three replications. The quality parameters of harvested seeds were measured by the NIR technique. Protein content increased but and fiber and water contents decreased significantly with an increase in the iron and phosphorus fertility rates. As the rates of foliar iron increased from 0 to 2 L/ha the mean values of protein content increased significantly from 13.47% to 15.48%. With increasing the rates of phosphorus from 0 to150 kg/ha, the mean values of protein content increased significantly from 13.56% to 15.53%, however, further increase in phosphorus, causing a decreased trend in the amount of protein. Fiber and water contents of wheat decreased significantly from 13.76% to 11.86% and from 7.36% to 7.28%, respectively, as the phosphorous rate increased from 0 to 225 kg/ha. Those changes were from 13.40% to 12.03% and from 7.57% to 7.21%, respectively, for increasing foliar iron from 0, 1.2, and 2 L/ha. Mathematical relationships composed of phosphorus and iron fertilizers were developed for accurately describing the wheat protein, fiber, and water contents under different fertilization rates.

Changes in grain protein and amino acids composition of wheat and rice under short-term increased [CO2 ] and temperature of canopy air in a paddy from East China.

Projected global climate change is a potential threat for food security. Both rising atmospheric CO2 concentrations ([CO2 ]) and temperatures have significant impacts on crop productivity, but the combined effects on grain quality are not well understood. We conducted an open-air field experiment to determine the impacts of elevated [CO2 ] (E-[CO2 ], up to 500μmolmol-1 ) and warming (+2°C) on grain yield, protein and amino acid (AAs, acid digests) in a rice-winter wheat rotation system for 2yr. E-[CO2 ] increased grain yield by 11.3% for wheat and 5.9% for rice, but decreased grain protein concentration by 14.9% for wheat and by 7.0% for rice, although E-[CO2 ] slightly increased the ratio of essential to nonessential AAs. With a consistent decline in grain yield, warming decreased protein yield, notably in wheat, despite a smaller increase in protein concentration. These results indicate that warming could partially negate the negative impact by E-[CO2 ] on grain protein concentration at the expense of grain yield; this tradeoff could not fully offset the negative effects of climate change on crop production.

Effects of long-term rotation and tillage practice on grain yield and protein of wheat and soil fertility on a Vertosol in a medium-rainfall temperate environment

Fundamental changes in farming systems occurred throughout the medium-rainfall zone of southern Australia during the late 1990s. Pulse and canola crops replaced pastures and long-fallowing, and minimal-tillage practices were increasingly adopted. An experiment was established in 1998 to examine long-term effects of these changes on crop productivity and soil fertility. Nine rotation–tillage treatments based on 3- and 6-year cycles were compared by using wheat (Triticum aestivum) as a bioassay crop over 2001–17. Seasonal conditions during the study ranged from the Millennium Drought to the top rainfall decile. Averaged across the 17 seasons, wheat yields were significantly lower in rotations based on continuous wheat (1.73t/ha) or 3 years of perennial lucerne (Medicago sativa) (1.93t/ha) and highest after traditional long-fallow (2.92t/ha) or vetch (Vicia sativa) green manure (2.57t/ha). Wheat yields following a pulse (2.23t/ha) or canola (Brassica napus) (2.21t/ha) were intermediate. Whereas rotation effects varied with seasonal rainfall, there was a long-term trend for relative yields in continuous wheat rotations to decline and those following a green manure or fallow to differ increasingly from other treatments. Compared across the same rotation (canola–wheat–pulse), average wheat yields under no-tillage (2.09t/ha) were significant lower (P<0.05) than under reduced (2.22t/ha) and conventional (2.29t/ha) tillage. By contrast, grain protein concentration increased with increasing proportion and type of legumes (green manure and pasture>pulse) in the rotation via their effect on soil mineral nitrogen (N). Lowest protein was recorded in continuous wheat and highest where at least one-third of the rotation contained a non-pulse legume, i.e. vetch green manure, lucerne or annual medic (Medicago truncatula). Soil-borne cereal fungal pathogens and nematodes generally had little effect on grain yields. Soil organic carbon (SOC) and total N stocks declined in all treatments over time, except in the lucerne and green manure treatments, where total N was maintained. Greatest declines in SOC and total N occurred in the fallow treatment. Results from the first 20 years of experimentation suggest that differences in grain yield (and protein) of wheat were most likely the result of treatment (rotation and tillage practice) effects on soil nitrate and water (growing-season rainfall and fallow storage) rather than disease. We found no evidence that current farming systems based on use of pulses, canola and reduced tillage are less able to maintain intervening cereal yields than traditional systems. On the contrary, practices such as long-fallowing are expected to have increasing negative influence on productivity in the longer term via decreased soil C and N fertility.

Nitrogen topdressing timing modifies free amino acids profiles and storage protein gene expression in wheat grain

BackgroundNitrogen is one basic element of amino acids and grain protein in wheat. In field experiments, wheat plants were subjected to different timing of nitrogen topdressing treatments: at the stages of emergence of the top fifth leaf (TL5), top third leaf (TL3) and top first leaf (TL1) to test the regulatory effects of nitrogen topdressing timing on grain protein quality. The underlying mechanisms were elucidated by clarifying the relationship between proteolysis in vegetative organs and accumulation of amino acids in the endosperm cavity, conversion of amino acids, and storage protein synthesis in endosperm of wheat grain.ResultsDelayed nitrogen topdressing up-regulated gene expression related to nitrogen metabolism and protease synthesis in the flag leaf, followed by more free amino acids being transported to both the cavity and the endosperm from 7 days after anthesis (DAA) to 13 DAA in TL1. TL1 enhanced the conversion between free amino acids in endosperm and upregulated the expression of genes encoding high molecular weight (HMW) and low molecular weight (LMW) subunits and protein disulfide isomerases-like (PDIL) proteins, indicating that the synthesis and folding of glutenin were enhanched by delayed nitrogen topdressing. As a consequense, the content of glutenin macropolymers (GMP) and glutenin increased with delaying nitrogen topdressing.ConclusionsThe results highlight the relationship between nitrogen remobilization and final grain protein production and suggest that the nitrogen remobilization processes could be a potential target for improving the quality of wheat grain. Additionally, specific gene expression related to nitrogen topdressing was identified, which conferred more detailed insights into underlying mechanism on the modification protein quality.

Nitrogen, Potassium, and Protein in Grains From Wheat Grown Under Nitrogen and Potassium Fertilizations in the Brazilian Cerrado

Nitrogen is a component of proteins contained in grains and potassium an enzymatic activator in nitrate (NO3-) assimilation and contributes to the translocation and storage of plant assimilates. Together they can increase protein contents of wheat grains. This research aimed to evaluate whether the interaction between nitrogen (N) and potassium (K) fertilizations in irrigated wheat in the Cerrado region of the Mato Grosso State increases the content and accumulation of N, K, and protein in wheat grains. The experiment was carried out in the field for two consecutive years (2014 and 2015) in the Federal University of Mato Grosso. It was designed in randomized blocks in 52 fractional factorial, composed of combinations between five doses of each of N and K, 13 treatments combinations in total with four replicates. After harvest, the grains were dried to determine the contents of N, K, and protein. The nitrogen content was influenced by nitrogen doses in both years and the accumulation significantly influenced by the nitrogen and potassium doses with an average increase among the years of 55.29% as a function of the potassium application. Nitrogen influenced the accumulation of potassium only in 2015 with effect for potassium in both years. Although there was no interaction between treatments, the influence of K on N absorption was evident. Contents and accumulations of N and K and the content of protein in wheat grains are influenced by N and K fertilizations.

Effects of zinc fertilizer and short-term high temperature stress on wheat grain production and wheat flour proteins

Content of wheat flour proteins affects the quality of wheat flour. Zinc nutrition in wheat can change the protein content of the flour. The inconsistency and instability of wheat grain quality during grain filling while under high temperature stress (HTS) are major problems in the production of high quality wheat. At present, there is a lack of studies on zinc fertilizer and HTS effects on wheat flour protein and the content of its components. For this study, treatment combinations of four levels of zinc fertilizers and exposure to a short-term HTS, at 20 d after flowering (D20), were tested on two wheat cultivars with different gluten levels. Individuals of a strong gluten wheat, Gaoyou 2018 (GY2018), and a medium gluten wheat, Zhongmai 8 (ZM8), were grown in pots at the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing in 2015–2017. We measured grain yield and weight and the activities of two enzymes (nitrate reductase and glutamine synthetase) from the flag leaves, collected at D10 and D20. Total protein content, protein yield, and content of four protein components (albumin, gliadin, glutenin, and globulin) were measured from flour produced from the pot-grown plants. HTS significantly increased the contents of total protein, albumin, gliadin, and glutenin in wheat grains, and reduced the grain yield, grain weight, protein yield, globulin content, and flag leaf nitrate reductase (NR) and glutamine synthetase (GS) activities. The results showed that HTS and zinc fertilizer had greater impacts on the strong gluten cultivar compared to the medium gluten cultivar. Under HTS, grain yield decreased by 13 and 8% in GY2018 and ZM8, respectively; protein yield decreased by 7 and 8% in GY2018 and ZM8, respectively. Zinc fertilizer increased: grain and protein yields; grain weight; total protein, albumin, gliadin, and glutenin contents; protein yield; and NR and GS activities. In contrast, zinc fertilizer reduced the content of globulin. The addition of 15 mg Zn kg–1 soil had the strongest effect on grain yield and quality as compared to the other three treatments (additions of 0, 30, and 45 mg Zn kg–1 soil). Zinc fertilizer also reduced the negative effects of HTS on protein yield, content, and components’ content. Therefore, wheat grown with additional zinc in the soil can improve the quality of the flour.

Nitrogen Flows in Agrocenosis of Winter Wheat Under Conditions of Different Reactions of Typical Chernozem Medium (Based on Application of 15N)

It is shown that an increase in winter wheat on typical chernozem characterized by the acid reaction of the soil medium leads to a decrease in grain yield to 30% and straw weight to 25%. The crop reached the maximum level after introducing urea and manure wastes (the yield gain was 199% in neutral soil and 165% in acidic soil). The improvement of plant nitrogen nutrition by introducing fertilizers led to an increase in the protein content in grain by 1.2 times on both soils; at the same time, the protein content was 1.1 times lower in acidic soil than in the neutral one. The maximum content of protein in winter wheat grain accumulated after both types of fertilizers were applied on neutral soil; the use of manure wastes alone decreased the protein content in grain. It has been revealed that a decrease in the yield of grain and a decrease in the content of crude protein in this grain after acidification of typical chernozem is due to a decrease in the amount of mineral nitrogen in soil and an increase in its gaseous losses. The pattern of nitrogen transformation in soil, as well as the availability of nitrogen for the plants, varied as the reaction of the medium changed. In acidic soil, the consumption of mineral fertilizer nitrogen and manure wastes by the plants decreased by 12–13% and the consumption of soil nitrogen decreased by 45%. The effect of wastes decreased the consumption of urea nitrogen by 49% (neutral soil) and by 69% (acidic soil). The consumption of fertilizer nitrogen by the plants decreased by 12–15% after acidifying typical chernozem and by 12–17% after immobilizing this type of chernozem and increased gaseous losses by 18–81%. It has been concluded that the agrophytocenosis of winter wheat maintains the ecological balance (homeostasis) when wastes from a pig-breeding complex are used and is exposed to stress when urea is applied in acid chernozem. Soil acidification decreased the resistance of agrophytocenosis to the level that is observed when manure wastes were applied and to adaptive depletion when urea was used.

ИСПОЛЬЗОВАНИЕ ПОКАЗАТЕЛЕЙ ОТНОСИТЕЛЬНОГО И АБСОЛЮТНОГО СОДЕРЖАНИЯ БЕЛКА В ЗЕРНЕ ОЗИМОЙ ПШЕНИЦЫ ПРИ СЕЛЕКЦИИ НА КАЧЕСТВО

The use of the indexes of relative and absolute content of protein in winter wheat grain selected on quality Winter wheat is the main food crop, which occupies a significant proportion in the structure of the grain area of Russia. The crop uses bioclimatic potential of the regions of cultivation better than spring wheat and supplies stable grain production. Winter wheat on Don supplies up to 50% of gross grain, and sometimes up to 70%. The article presents the study results of the accumulation of relative and absolute protein content in grain depending upon 1000-kernel weight on average during the years of 2014-2016. The obtained data indicate that absolute protein content in grain does not depend on the productivity. This index is really stable. Such indexes as 1000-kernel weight, relative protein content in grain, gross yield of protein per hectare, winter wheat productivity are significantly changeable. Protein content in grain is slightly affected by weather conditions. There is an inverse relation between 1000-absolutely dry kernel weight and relative protein content in grain. The more 1000-absolutely dry kernel weight is, the less protein content in grain is and vice versa. But there are significant differences of the trait among the varieties. The varieties “Rostovchanka 5” and “Rostovchanka 7” with less 1000-kernel weight than the varieties “Tanais”, “Aksiniya” and “Nakhodka”, have formed less protein content through all the years. The varieties “Tanais”, “Aksiniya” and “Nakhodka” with 1000-kernel weight of 41.1-43.7 g produced the largest amount of protein (14.7%, 14.4% and 14.2% respectively).

Phytochemical properties and heavy metal accumulation in wheat grain after three years’ fertilization with biogas digestate and mineral waste

Non-standard materials used for plant fertilization, require characterization to reduce any possible undesired effects. The aim of this study was to investigate the effect of fertilization with biogas digestate (BD) and mining waste (carboniferous mudstones (MS) from coal mine) on wheat yield quality and the bioaccumulation of heavy metals in wheat grains in relation to conventional fertilization (NPK) and no fertilization. Using the co-application of waste (MS+BD), the highest yield was obtained in the first and second year, but in the third year, a slight decrease in yield was observed compared to NPK. In all years, BD and MS+BD fertilization increased the content of protein, wet gluten, and phenols in wheat grain compared to NPK and no fertilization. Waste fertilization had a positive effect on the antioxidant capacity index of grain but only in the first year. The bioaccumulation index (BAI) demonstrated that NPK decreased BAI for Zn, B, Cd, and Mn, whereas MS+BD decreased BAI for Cu, Sr, Pb, Co, Ba, and Cr.

Enhancing Wheat Production Under Arid Climate Stresses Using Bio-Elicitors

Arid climate has contributed to crops yield production decrement in many regions due to water shortage and soil salinization. The main target of this research is to enhance wheat production under arid climate stresses by utilizing bio-elicitors. Two common wheat assortments i. e. cv. Gemmiza 10 and Sakha 93 accomplished tolerant to these stresses utilizing bio elicitors include Acremonium coenophiulum, Streptomyces griseus, Trichoderma harzanium, T. viride, Rhodotorula glutinis, Paenibacillus polymyxa, Bacillus subtilis, B. megaterium, Pseudomonas putida, P. fluorescens compared with natural elicitors as methyl jasmonate (MJ), chitosan (CHI), ascorbic acid and putrescein (PUT). Varieties were planted in two progressive seasons in recently reclaimed lands, where two fields were selected the first is located in North Sinai while the second is situated to the south of Behira Governorate. Bio elicitors reduced abiotic stress and enhance physiological characteristic expressed as Proline, soluble carbohydrates %, Chl.a, Chl.b, Chl.a + b; Chl.a/Chl.b; Carotenoids and increased the concentration of Crude protein, N, P and total soluble carbohydrates in wheat grains. Using P. fluorescens exhibted increment in grain yield of wheat plants, cv sakha 93 amounted by 68.3% in Sinai region, while in Behira region the increement reached (45.5%) with P. polymyxa treatment. The response of Gemmiza 10 variety to the Bio-elicitors was less compared to Sakha 93 variety in both regions, where the highest increments due to P. putida treatment were18.9 and 19.7% in Sinai and beheirwa regions, respectively, compared to the control in each region. A remarkable increments of wheat growth, development and extended survival under salt and water limiting and restricting conditions. These findings demonstrate that wheat plants can display improved stress tolerance through bio-elicitors, and recommend that innovation may be useful in reducing effects of climate change and environmental stress on other crops and expanding agricultural production onto marginal lands.

Spatial distribution patterns of protein and starch in wheat grain affect baking quality of bread and biscuit

To evaluate the spatial distribution of chemicals and their contribution to processing quality, wheat grains were layered into nine fractions from the surface to the center layer. The content of total starch, amylose and amylopectin gradually increased from the surface layer to inner layer, while albumin, globulin and total protein decreased in this direction. Contents of gliadin, glutenin, high molecular weight glutenin subuints (HMW-GS) and glutenin macropolymers (GMP) increased from the surface layer, and peaked at the second or the third pearling layer, and then decreased till the center layer. The mean particle diameter of starch granules increased gradually from the surface to inner except at P9. Bread baking performance was the best for flour at the mid layers, while was the worst for flour from the surface layer, especially in terms of taste and texture. Biscuit making performance was the best at the center layers, while poorest at the surface layer.

Effects of Nanochitin on the Enhancement of the Grain Yield and Quality of Winter Wheat

This study investigated the effects of different rates of nanochitin in soil on the grain yield and quality of winter wheat. Nanochitin obtained by acidic hydrolysis of shrimp chitin was a rod-like whisker possessing a hydrodynamic diameter of 143 nm and ζ potential of 55.7 mV. Two varieties of winter wheat, multi-spike wheat (MSW) and large spike wheat (LSW), were treated with the nanochitin suspension in outside pot experiments. The results showed that 0.006 g kg-1 of nanochitin in soil could significantly enhance the yield by 23.0% for MSW and 33.4% for LSW, with significant increases of net photosynthesis rate, stomatal conductance, intercellular CO2 concentrations, and transpiration rate in flag leaf at the grain filling stage. Grain protein, iron, and zinc contents in wheat treated with nanochitin were also increased by 5.0, 10.3, and 22.1% for MSW and 33.4, 32.0, and 27.0% for LSW, respectively. This indicated that utilization of nanochitin has a great potential in future agriculture sustainability and crop production.