Dietary Protein Source Research Articles

Carbon-concentrating mechanisms in pods are key elements for terminal drought resistance in Phaseolus vulgaris.

Common bean (Phaseolus vulgaris L.) is one of the most consumed legumes in the human diet and a substantial source of dietary protein. A major problem for this rainfed crop is the decrease in grain yield caused by prolonged drought periods during the reproductive stage of plant development (terminal drought). Terminal drought remains a prevailing threat to the farming of this staple, with losses reaching >80%. Based on the high correlation between the resistance of common bean to terminal drought and efficient photoassimilate mobilization and biomass accumulation in seeds, we aimed to identify mechanisms implicated in its resistance to this stress. We used two representative Durango race common bean cultivars with contrasting yields under terminal drought, grown under well-watered or terminal drought conditions. Using comparative transcriptomic analysis focused on source leaves, pods, and seeds from both cultivars, we provide evidence indicating that under terminal drought the resistant cultivar promotes the build-up of transcripts involved in recycling carbon through photosynthesis, photorespiration, and CO2-concentrating mechanisms in pod walls, while in seeds, the induced transcripts participate in sink strength and respiration. Physiological data support this conclusion, implicating their relevance as key processes in the plant response to terminal drought.

The formaldehyde treated guar meal and prill fat, agro industrial by-products as dietary rumen protected protein and energy source: improves growth performance, feed conversion, nutrient utilization, microbial protein synthesis, blood metabolites and economic efficiency in growing dairy buffalo (Bubalus bubalis) calves.

The study investigated the effects of protein replacement with formaldehyde-treated guar meal (FTGM) and prill fat (PF) in the diet on performance of growing dairy buffalo calves. Thirty-two feedlots Surti breed dairy buffalo calves (age, 7.31 ± 0.34months and body weight, 90.69 ± 6.19kg) were assigned into four dietary treatments (n-8 calves/each): (1) control group, supplied basal diet as per ICAR (2013) nutrient requirements; (2) FTGM group, 30% crude protein (CP) requirement of concentrate mixture (dry matter basis (DMB)) replaced with FTGM in basal diet; (3) PF group, supplied basal diet + 100g PF; and (4) FTGM + PF group, 30% CP requirement of concentrate mixture (DMB) replaced with FTGM in the basal diet + 100g PF for 280days. All the treatment diets were isonitrogenous. Growth performance was improved in FTGM + PF and FTGM groups. Apparent digestibility (%) of CP was increased in FTGM and FTGM + PF diet, while digestibility (%) of ether extract (EE) was increased in PF group. Serum total protein, albumen, urea nitrogen, and creatinine concentrations were higher in FTGM + PF and FTGM groups, whereas total cholesterol and triglycerides levels were greater in FTGM + PF and PF groups. Calculated methane emission had a discernible influence of treatment in FTGM and FTGM + PF. The overall cost of feeding per kilogram gain was lowest in FTGM and FTGM + PF groups. In conclusion, 30% CP replacement with FTGM with or without PF improved the growth performance, feed conversion ratio, and nutrient utilization; supported efficient utilization of resources; and economized the rearing of growing dairy buffalo calves.

Bioactive properties of peptide fractions from Brazilian soy protein hydrolysates: In silico evaluation and experimental evidence

Soybeans are a known source of dietary proteins and potential bioactive peptides. In this study, a protein hydrolysate from soybean protein concentrate was produced using papain. The peptides were separated by ultrafiltration (< and > 3 kDa, LMMH (low molecular mass) and HMMH (high molecular mass), respectively) and sequenced through LC-MS/MS. To obtain a thorough identification of the peptides in the hydrolysate, different analysis methods and bioinformatics techniques were applied, covering a molecular mass range from more than 480 Da up to small dipeptides. The antioxidant and the inhibitory α -glucosidase and lipase potentials were evaluated by different in vitro tests. Sixty-nine peptides were identified in the HMMH fraction and 32 in LMMH, but only 16 matched the 118 sequences obtained by in silico simulated hydrolysis. Unlike previous reports, the HMMH fraction showed higher antioxidant activity, by all 5 in vitro methods applied, which was not accompanied by the in silico evaluation. Both high and low molecular mass fractions showed similar inhibitory activities against α -glucosidase and pancreatic lipase. LMMH, however, showed better results for α -glucosidase inhibition (IC50 = 0,94), in agreement with the in silico evaluation. This combination of bioactivities makes the fractions of this hydrolysate potential food ingredients with the possible ability to delay the lipid peroxidation of meat products, limiting the digestion of lipids in the product and also with the potential to delay the digestion of carbohydrates ingested in the same meal.

Updates on cowpea viruses in Southwest Nigeria: distribution, prevalence and coinfection.

The online version contains supplementary material available at 10.1007/s42360-022-00576-8.

Effects of Blood Meal Inclusion Diet on Growth Performance, Feed Utilization and Survival Rate of Clarias gariepinus (Burchell 1822) in Concrete Pond

The inclusion of blood meal as a substitution for commercial fishmeal in the practical fish diets was evaluated in Clarias gariepinus juveniles (initial weight: 11.45±0.25g and initial length: 9.00±0.91cm) for a period of 39 weeks. The proximate analysis of blood meal had 50.04%, 4.95%, 0.98%, 3.05%, 16.51% and 19.00% representing value of crude protein, lipid, fibre, ash, moisture and nitrogen free extract, respectively. Five isonitrogenous diets (40% crude protein) containing fishmeal which was replaced by blood meal at graded level of 0% as control, 25%, 50%, 75% and 100% were formulated. A total of 300 juveniles were used and randomly distributed into five treatments with each having triplicates of 20 fish per pond respectively. Fish were fed twice daily at 5% body weight in equal proportions at 8.00 am and 4.00 pm. At the end of the feeding trial, the results showed that growth performance were significant higher (p&lt;0.05) in fish fed control diet (1263.55g) and 25% BM diet (1068.67 g), mean length gain (54.16 cm and 50.85 cm), protein efficiency ratio (31.59% and 24.22%) and percentage weight gain (11035.3% and 8476.03%) compared to other treatments diets with the lowest values of (675.35 g, 46.86 cm, 11.73% and 4190.63%, respectively) recorded in fish fed 100% BM diet. However, Feed conversion ratio, specific growth rate, average daily growth and survival rate were not significantly difference (p&gt;0.05) in all the dietary treatments. The water quality parameters monitored were within recommended ranged and were not affected by the supplemented diets. It can be concluded that inclusion of blood meal up to 25% can efficiently replace fish meal without any deleterious effects on growth, feed utilization and survival rate. The use of blood meal as an alternative protein source in diets of farmed fish species will reduce the cost of feeds; boost the profitability and sustainability of the fish production.

Contrasting Male and Female Dietary Life Histories: A Case Study at an Ancestral Muwekma Ohlone Heritage Site in San Jose, California

ABSTRACT: Stable carbon and nitrogen isotope analyses of bones and teeth at the ancestral heritage Muwekma Ohlone site of Yakmuy ́Ooyákma-tka (“Place of the East Ridge Site”; CA-SCL-215) reveal significant differences in the dietary life history of males and females. Overall, isotope data indicate that site inhabitants were primarily dependent on low-trophic-level foods, likely plants, and minor amounts of marine food for their main source of dietary protein. From tooth dentin serial samples, we found that males and females were similar for δ13C in early childhood (age 1–9 years), but boys were elevated in δ15N by 0.6–1.0‰ relative to girls, indicating boys were accessing slightly greater amounts of higher-trophic-level foods, such as meat from game. The sex-biased difference in δ15N diminishes during the second decade of life, as female δ15N values increase and become equal to males. However, a difference in δ13C emerges during the second decade: female δ13C values are elevated relative to males. This could indicate that teenage females consumed higher amounts of low-trophic-level, marine-derived protein, such as shellfish. During later adult years the difference in δ13C disappears, while males again show an increase in δ15N relative to females. Although these differences are small, they reveal important sex-biased life history patterns during childhood and adulthood in this ancient community.

High-risk meat and fish cooking methods of polycyclic aromatic hydrocarbons formation and its avoidance strategies

According to recent research, human exposures to polycyclic aromatic hydrocarbons (PAHs) are mainly through the food route. Food such as meat and fish are two primary sources of dietary protein. However, a certain level of unawareness and poor cooking education leads to the formation of PAHs in cooked meat and fish meals. PAHs are classified as human carcinogens that poses a risk of cancer and related diseases. This review studied high-quality published articles on cooked meat and fish, emphasizing the level of PAHs contaminations, cooking methods, and related parameters. Additionally, verities of easy-feasible PAHs avoidance strategies for meat and fish cooking are summarized. Meat and fish cooking methods such as smoking, grilling, barbecuing, roasting, and traditional frying amalgamated with the risk of PAHs contamination. In accordance with expectations, there are some strategies to avoid PAHs contamination in cooked meat and fish up to 100%, including low-fat content, fuel/wood types, smoking filters, and different types of marination. This study comprehensively identified the manner in which PAHs avoidance strategies aligned with present safety guidelines.

Lab-based meat the future food

• Lab-based meat is more sustainable and safer to consume than conventional meat. • Production steps: cell extraction, cell line establishment, cell culture and scaffolding. • Challenges: technology limitations, consumer acceptance & law and legislation. Meat is one of the main dietary protein sources that is important in human health and development. Today, the rapid growth of the world's population has resulted in an increasing demand for meat sources worldwide. With the climate crisis devastating natural and agricultural resources, the Earth's ecosystems may no longer support an expanded traditional meat industry. At the same time, increasing meat production and consumption have raised issues regarding the environment, animal ethics, and human health. In line with the global sustainability trends, lab-based meat has been introduced in the last decade as a meat alternative. The production of lab-based meat required high-throughput technology at a wide scale to develop high-quality meat in the laboratory customized for the livestock agriculture industry, environment, animal welfare and human health. However, the development of laboratory-produced meat is still in its early stages, and it is currently confronted with significant challenges such as technological limitations and consumer acceptance. Therefore, there is no legal recognition of lab-based meat worldwide. Nevertheless, due to the trend for a high market demand for meat analogues, this field of study is expected to contribute to the company portfolio, thus more funding and research are required to ensure its safety and societal requirements.

DEVELOPMENT OF PROTEIN ENRICHED SOUP POWDER BY USING PRAWN AND SHRIMP

Protein is a macronutrient that is essential for building muscle mass. Prawn and shrimp is a good source of nutrients. Instant soup is a type of soup designed for fast and simple Preparation. Some are home made, and some are mass produced on an industrial scale and treated in various ways to preserve them. A wide variety of types, styles and flavors of instant Soups exist. Commercial instant soups are usually dried or dehydrated, canned, or treated by freezing. Dried prawn and shrimp is extremely high in protein, up to 93% of calories. Seafood is an excellent Source of protein because it’s usually low in fat. Prawn and shrimp are good sources of dietary protein while also supplying essential minerals and heart-healthy omega-3 fatty acids. Protein enriched instant soup is done by prawn and shrimp and dehydrated vegetables. Prawn and Shrimp were subjected to tray drying and grinding. The observed high protein content in product will find promising application in food products. Powder from both Prawn and shrimp varieties were analyzed for proximate Analysis, powder properties and nutritional content. The protein content is observed to be high in the prawn and shrimp powder compared to raw prawn and shrimp. Nutritional parameters analyzed were Protein, Total fat, Carbohydrates, Minerals and Heavy metals. Prawn and shrimp powder had significantly higher protein content apart from other parameters. This finds scope in designing high.Soup mix was Formulated and optimized by RSM software. Analyses were done to the soup mix.

Dietary Plant Protein Intake Can Reduce Maternal Insulin Resistance during Pregnancy

Evidence suggests that the source of dietary protein may have an impact on insulin resistance, but no studies have explored it in pregnant populations. In this study, we combined a population study and an animal experiment to explore this effect. The population study was conducted with data from NHANES. Multiple linear regression was used to observe the association of protein intake with outcomes, including fasting glucose (GLU), insulin (INS), and HOMA-IR. In the animal experiment, 36 pregnant SD rats in three groups were orally administered 100% animal protein, 50% animal protein and 50% plant protein, or 100% plant protein, respectively. The intervention continued throughout the whole pregnancy. On day 19.5, maternal plasma was collected after overnight fasting, and metabolomics was performed using UPLC-MS. We found plant protein intake was negatively correlated with INS and HOMA-IR in the whole population. During the third trimester, a similar correlation was also observed. The animal experiment also presented the same result. In metabolomic analysis, changes in various metabolites and related pathways including FoxO and mTOR signaling pathways were observed. In conclusion, we found a negative association between dietary plant protein intake and maternal insulin resistance during pregnancy. Changes in some active substances and related metabolic pathways may play an important role.

Reassessing Neolithic Diets in Western Scotland

Although marine resources are known to have been exploited by both foragers and early farmers in Scotland, the importance of seafood to the diets of Neolithic groups has been widely debated. Here we present paired stable isotope (δ13C and δ15N) and radiocarbon measurements on Early Neolithic human remains from Raschoille Cave in Oban. These are compared with published data for other sites in western Scotland and used to re-evaluate the use of marine resources by the first farmers. The diets of Late Mesolithic foragers and Early Neolithic farmers were modelled from stable isotope data using both Linear and Bayesian (FRUITS) mixing models. Our FRUITS dietary models indicate that Mesolithic foragers obtained much of their dietary protein and calories from marine resources, consistent with the predominance of shellfish, fish and sea mammal remains in their shell middens. Of note is the large proportion of dietary calories obtained from plant foods, which is like that of the early farming groups. The diets of Early Neolithic farmers appear relatively homogeneous across Scotland. Plant foods were the primary source of calories. Meat and/or dairy from terrestrial mammals were the most important source of dietary protein. Marine resources were, at most, a minor component of the ‘lifetime’ diet.

Evaluating Artemia biomass and gut weed (Ulva intestinalis) meal as a dietary protein source for black tiger shrimp (Penaeus monodon) postlarvae

A 45-day feeding trial was conducted to assess the influence of Artemia (Artemia franciscana Vinh Chau) biomass and gut weed (Ulva intestinalis) meal as replacement protein sources for fishmeal (FM) and soybean (SB) protein on the performance of black tiger shrimp (Penaeus monodon) postlarvae (PL). The control diet did not include Artemia biomass or gut weed meal, and the experimental feeds were formulated to replace 20%, 40%, 60%, and 80% of the FM protein with Artemia biomass meal, combined with the replacement of 15%, 30%, 45%, and 60% of SB protein with gut weed meal. All the experimental feeds were similar in crude protein (40%) and lipid (7%) content, with three replicates per treatment. Shrimp PL with an average weight of 0.019 ± 0.001 g and length of 1.17 ± 0.02 cm were reared at a salinity of 10 ppt. The results showed that the survival of the shrimp was not significantly influenced by the feeding treatments (P > 0.05) and ranged from 81.1% to 86.7%. Interestingly, the growth rates in terms of the weight, feed efficiency, and resistance to formalin shock of the shrimp that were fed Artemia biomass and gut weed meal were superior to those that received the control feed. The study findings demonstrated that Artemia biomass combined with gut weed meal can replace up to 80% FM protein and 60% SB protein in the P. monodon PL diet. Notably, optimal shrimp performance wasobtained using a 40% Artemia biomass and 30% gut weed protein replacement diet.

Feed Efficiency, Tissue Growth and Energy Budget Changes during the Molting Cycle of Juvenile Mud Crab, Scylla serrata: Effects of Dietary Proteins, Fishmeal versus Soy Protein Concentrate

Three isoenergetic diets differing in their fishmeal/soy protein concentrate (SPC) ratio were assessed on the tissue growth and energy budget of juvenile crabs Scylla serrata in postmolt stages (PMolt) and in intermolt stages (IMolt). The average growth rates on a dry matter basis were 2.064 ± 0.324% and 0.492 ± 0.08% initial BW.day−1 during PMolt and IMolt stages, respectively. The efficiencies of the feed conversion (FCE, %), protein retention (PRE, %) and energy retention (ERE, %) were similar for the three experimental diets. However, FCE, PRE and ERE in PMolt stages were four to five times higher than in IMolt stages. The feed intake, energy and protein required for growth in PMolt stages were obviously higher than in IMolt stages. The energy budgets (% total energy intake) were marginally affected by diet but were significantly affected by the molt stage. The maintenance energy was lower in PMolt stages (49.84 ± 4.9%) than in IMolt stages (83.33 ± 2.45%). The excess in maintenance energy in IMolt stages represents the portion set aside for the next molt: shell energy content (4.97 ± 0.31%) and energy for ecdysis (±28%). Conversely, recovery energy was significantly higher in PMolt stages (34.39 ± 0.99%) than in IMolt stages (8.33 ± 1.7%). In conclusion, SPC sustained good tissue growth and good feed utilization and can be used as a main source of dietary protein for crab juveniles in captivity.

Effects of dietary plant and animal protein sources and replacement levels on growth and feed performance and nutritional status of market-sized turbot (Scophthalmus maximus) in RAS

One part of aquaculture sustainability is reducing the environmental footprint of aquaculture feeds. For European aquaculture, this means finding feed ingredients that are produced within the economic community, and that are not in conflict with human consumption. This is especially challenging when formulating diets for carnivorous fish such as turbot with low tolerance to fishmeal replacement that are both nutritious and economically and environmentally sustainable. Therefore, we investigated the effects of two novel and innovative feed formulation concepts on growth and feed performance and the nutritional status of market-sized turbot in a recirculating aquaculture system. In a 16-week feeding trial, 440 turbot (300 ± 9 g) were fed twice a day with a control diet (CTRL), based on a commercial formulation, and four experimental diets. The experimental diets were designed to investigate the effects of two formulations concepts based on sustainable terrestrial plant proteins (NoPAP) or processed animal proteins (PAP) and of 30% and 60% fishmeal replacement with emerging feed ingredients (fisheries by-products, insect meal and fermentation biomass). Turbot from the CTRL group had a similar growth and feed performance than fish fed the NoPAP30 formulation, with a significant decline of performance in the fish fed both PAP formulations and the NoPAP60. Comparing the two formulation concepts with each other the voluntary feed intake and protein efficiency ratio on tank basis as well as the individual weight gain and relative growth rate was significantly higher in the fish from the NoPAP groups than PAP groups. Furthermore, the apparent digestibility of nutrients and minerals was significantly reduced in the fish fed with the diets with 30% and 60% fishmeal replacement level compared to the fish from the CTRL group. In conclusion, the performance of the fish fed the NoPAP30 formulation concept highlights the potential of the used combination of sustainable ingredients, such as fisheries by-products, insect meal, microbial biomass and plant protein for turbot. Furthermore, this study shows that turbot has a higher tolerance to the incorporation of plant and insect protein than of processed animal protein.

A Review: Evaluation of Black Soldier Fly (Hermetia illucens) Larvae Meal as a Dietary Protein Source in Poultry Diets

The increasing global population poses a problem in achieving food security. Poultry is one of the major animal protein sources for humans and the main difficulty of the poultry industry is seeking a suitable protein alternative to be integrated into poultry feed. Black soldier fly larvae (BSF) (Hermetia illucens) are one of the edible insects that are a great solution to this problem since they have various advantages, which include bioconversion of different organic wastes and the absence of competition for human resources. Several studies have already examined the effects of the addition of BSF larvae in the poultry diet, and most suggested that it is a promising alternative protein source that can replace soy or fish meal. The BSF larvae can also be used in different species of poultry, such as ducks, turkeys, and quails. However, there are several barriers to using BSF larvae as a feed ingredient in poultry feed, such as safety, price, and consumer acceptance. Despite these obstacles, BSF larvae production is still a promising industry due to the numerous benefits it brings.

Association Between Dietary Protein Sources and Gut-Derived Uremic Toxins in Patients on Long-Term Hemodialysis

Association Between Dietary Protein Sources and Gut-Derived Uremic Toxins in Patients on Long-Term Hemodialysis

Gut-Immune-Kidney Axis: Influence of Dietary Protein in Salt-Sensitive Hypertension.

Humans with salt-sensitive hypertension demonstrate increased morbidity, increased mortality, and renal end-organ damage when compared with normotensive subjects or those with salt-resistant hypertension. Substantial evidence from humans and animals has also demonstrated the role of dietary components other than salt to modulate hypertension. Evidence presented in this review provides support for the view that immunity and inflammation serve to amplify the development of salt-sensitive hypertension and leads to malignant disease accompanied by end-organ damage. Interestingly, salt-sensitive disease is modulated by changes in dietary protein intake, which also influences immune mechanisms. Together, the evidence presented in this review from animal and human studies indicates that changes in dietary protein source have profound effects on the gut microbiota, microbiota-derived metabolites, DNA methylation, gene expression, immune cell activation, the production of cytokines and other factors, and the development of salt-sensitive hypertension and related disease phenotypes.

Toward Feeds for Circular Multitrophic Food Production Systems: Holistically Evaluating Growth Performance and Nutrient Excretion of African Catfish Fed Fish Meal-Free Diets in Comparison to Nile Tilapia

In aquaponics and circular multitrophic food production systems, dietary protein source, as well as fish species choice, particularly in cases of different nutritional physiology, could be factors affecting excreted nutrient profiles. Accordingly, growth performance, dissolved nutrient accumulation and feces nutrient profiles were evaluated for African catfish (Clarias gariepinus) reared in recirculating aquaculture systems (RAS) and fed single protein source diets based on black soldier fly larvae meal (BSF), poultry by-product meal (PM), poultry blood meal (PBM) and fish meal (FM) and the results were compared to previous findings for Nile tilapia (Oreochromis niloticus). All diets resulted in significantly different growth performances of African catfish, with FM producing the best growth performance, followed by PM, BSF and PBM. PM resulted in the highest soluble reactive phosphorus concentrations (SRP) in the RAS water; whereas, BSF resulted in the highest K, Mg and Cu concentrations. The highest feces nutrient density was recorded for PBM; whereas, FM and PM yielded the lowest feces nutrient density. Comparing African catfish to Nile tilapia revealed that the former showed significantly better growth performance with FM and PM, however, significantly weaker performance with BSF. Although dissolved K accumulation was similar between species across diets, significant differences were recorded for total inorganic nitrogen and SRP production per unit of feed for individual diets. Despite similar feces nutrient profiles, African catfish produce significantly less feces dry matter per unit of feed for each diet compared to Nile tilapia. Findings are discussed regarding their implications for aquafeed development in the context of circular multitrophic food production systems.

Effects of Dietary Cottonseed Protein Concentrate Levels on Growth Performance, Health Status, Flesh Quality and Intestinal Microbiota of Grass Carp (Ctenopharyngodon idellus).

The aim of this study was to evaluate the nutritional value of cottonseed protein concentrate (CPC) as a single dietary protein source and the optimal protein level for grass carp (Ctenopharyngodon idellus). An 8-week feeding trial was conducted by feeding juvenile grass carp (initial body weight: 4.68 ± 0.01 g) with six experimental diets containing graded levels of protein provided by CPC. The results showed that the optimal CPC level (CPC4) improved the growth performance and health status of grass carp. The optimal dietary protein level was estimated to be 38.61 and 38.66% based on specific growth rate (SGR) and feed efficiency (FE), respectively. The CPC4 group significantly increased the total antioxidant capacity (T-AOC) content and glutathione peroxidase (GSH-Px) activity in the hepatopancreas (p &lt; 0.05). In addition, the CPC4 group increased the muscle T-AOC and glutathione (GSH) content and improved muscle hardness, and the gene expression of MRFs, fgf6a, myhc-7, myhc-1, myhc-4, igf-II, and tor was upregulated while mstn gene expression was downregulated (p &lt; 0.05). Correlation analysis revealed that the optimal dietary CPC level promoted grass carp growth, health, and flesh quality by regulating the relative abundance of intestinal microbes. Furthermore, CPC6 upregulated the ko00480 (Glutathione metabolism) and ko00620 (Pyruvate metabolism) pathways compared to CPC1 (p &lt; 0.05), possibly indicating that low dietary CPC levels adversely affected amino acid metabolism in the intestinal microbiota of grass carp, while a high level of CPC will meet the metabolic needs of the body by increasing the utilization of energy.

High-Fat Dairy Products May Decrease the Risk of Chronic Kidney Disease Incidence: A Long-Term Prospective Cohort Study

The association between consumption of dairy products and risk of chronic kidney disease (CKD) is under debate. We aimed to determine the potential effects of total and subtypes of dairy intake on the occurrence of CKD. This study was conducted within the Tehran Lipid and Glucose Study (TLGS) on 2416 CKD-free adults. At baseline, consumption of dairy products was estimated using a validated 168-items semiquantitative food frequency questionnaire. Adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of CKD were calculated in tertile categories of dairy products. Also, the CKD risk was estimated with multivariable Cox regression to substitute total dairy with other dietary protein sources. During 8.4years of follow-up, the incidence rate of CKD was 21%. The participants' mean (±SD) age was 38 (±13) years and 46% were men. Dietary intakes of total dairy, low-fat dairy, and fermented dairy were not associated with CKD risk. There were significant lower risks of CKD in the highest compared to the lowest tertiles of high-fat dairy (HR=0.76, 95% CI=0.60-0.95) and high-fat milk (HR=0.75, 95% CI=0.59-0.96). However, no significant associations were found between other categories of dairy products and CKD incidence. Substitutions of total dairy with other dietary protein sources were not associated with CKD risk. In this study, higher intakes of high-fat dairy and high-fat milk were associated with lower risks of CKD. No significant associations were found between other dairy products and CKD. More prospective and clinical trials are needed to clarify the issue.