A method, devised by Dr. M. Wada, for collecting the bile flowed out from the duodenal papilla in a non-fastened, non-anesthetized dog, without interfering with the function of the gall bladder and the Oddi's sphincter, is described. Effect on bile discharge from the papilla of food-stuffs, beef, bread and milk, in the amount corresponding to one-third of the basal metabolism pet diem r_??_ fuel value. One hour observation before feeding yielded no output of bile. On giving food the bile starts to pour out from the duct with a long latency, say-about half an hour in cases with beef or bread and a quarter hour with milk. The rate of discharge increases, so that its acme is reached at the former half of the second hour, and then diminishes step by step, but five hours are not enough to see stoppage of discharge. In the ascending and descending limbs of the output curve there are some fluctuations, but never significant. The protein rich diet apparently has a somewhat powerful influence upon the rate of output, but never so significant, and the time course of discharge is almost the same for all the kinds of food-stuffs. This makes a striking contrast against the different effect of a kind of food-stuffs, wit off, of the carbohydrate rich diet, upon the gastric and pancreatic secretion other than the remaining kinds of stuffs. This point is discussed in full in text.