Protection Protocols Research Articles

Factors Influencing Emergence Timing Patterns of Long-Tailed Bats in Exotic and Native Forest in New Zealand.

Understanding temporal behavioural patterns in animals can be crucial to their conservation management. Emergence timing in bats, that is, the decision on when to depart day-roosts for foraging, is one such example and is well studied in Northern Hemisphere bats. The emergence timing of New Zealand long-tailed bats (Chalinolobus tuberculatus) is not yet fully understood, including when and where they may be vulnerable to threats. We investigated factors influencing long-tailed bat emergence timing in the Kinleith Forest (exotic plantation, 38° S) and the Eglinton Valley (native beech forest, 45° S). We recorded emergence times during late pregnancy through post-lactation (October to March), to determine whether the month, temperature at sunset, tree density, cloud cover, presence of rain and the number of bats within a roost influenced emergence timing. Most long-tailed bats emerged after sunset in the Kinleith Forest, whilst 80% of first emerging bats departed before sunset in the Eglinton Valley where nights are much shorter in summer, reducing foraging time. Month, temperature at sunset, and roost population size were the most important predictors of emergence timing at both sites. Long-tailed bats in the Kinleith Forest also emerged earlier as tree density increased, a pattern potentially associated with predator defence. The factors influencing long-tailed bat emergence timing are likely context dependent, namely latitude and habitat structure, which has implications for roost protection protocols, timing of bat surveys and interpretation of bat acoustic monitoring data.

International Refugee Law and Bangladesh: Navigating Sovereignty and Humanitarian Obligations

This article critically evaluates the intricate navigation between national sovereignty and international refugee law in Bangladesh, specifically concentrating on the nation’s mechanisms of the protracted Rohingya refugee crisis. As a non-signatory to the 1951 Refugee Convention, Bangladesh's legal obligations are initially motivated by the influence of international humanitarian norms, regional considerations, and domestic policy frameworks. This paper examines Bangladesh’s efforts to mediate its sovereign prerogatives with its moral and legal obligation to serve sanctuary to displaced populations. Through critical evaluation of legislative endeavors, governmental reactions, and the efforts of international actors such as the UNHCR, the article enunciates Bangladesh's multifaceted obstacles in its mandate to develop global refugee standards while indicating its domestic needs. The study articulates by proffering strategic suggestions aimed at liberalizing Bangladesh’s mechanisms with international refugee protection protocols, thereby contributing to both the strategy on refugee administration and the progression of legal mechanisms in South Asia.

Mars Sample Return: From collection to curation of samples from a habitable world

NASA’s Mars 2020 mission has initiated collection of samples from Mars’ Jezero Crater, which has a wide range of ancient rocks and rock types from lavas to lacustrine sedimentary rocks. The Mars Sample Return (MSR) Campaign, a joint effort between NASA and ESA, aims to bring the Perseverance collection back to Earth for intense scientific investigation. As the first return of samples from a habitable world, there are important challenges to overcome for the successful implementation of the MSR Campaign from the point of sample collection on Mars to the long-term curation of the samples on Earth. In particular, the successful execution of planetary protection protocols adds well-warranted complexity to every step of the process from the two MSR Program flight elements to the ground element at the sample receiving facility (SRF). In this contribution, we describe the architecture of the MSR Campaign, with a focus on infrastructure needs for the curation (i.e., the clean storage, processing, and allocation) of pristine Martian samples. Curation is a science-enabling and planetary protection-enabling activity, and the curation practices described in this contribution for the SRF and any long-term curation facility will enable the sample safety assessment, initial scientific investigations of the samples, and establish the MSR collection as a scientific resource that will enable generations of science and discovery through studies of the returned Mars samples. The planetary protection and curation processes established for MSR will provide critical insights into potential future sample return missions from other habitable worlds like Enceladus and Europa.

Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods.

In vivo calcium imaging in freely moving rats using miniscopes provides valuable information about the neural mechanisms of behavior in real time. A gradient index (GRIN) lens can be implanted in deep brain structures to relay activity from single neurons. While such procedures have been successful in mice, few reports provide detailed procedures for successful surgery and long-term imaging in rats, which are better suited for studying complex human behaviors. We present a robotic surgical protocol for same-day virus injection and GRIN lens implantation into the rat nucleus accumbens core. Our procedure utilizes a direct lens insertion without tissue aspiration and produces quality image retention for months of recording. We also describe daily recording strategies to minimize damage and promote long-term imaging. Finally, we present custom protective strategies to eliminate the need to remove miniscopes between sessions. This methodology protects rats from repeated isoflurane exposure and ensures a consistent focal plane for the entirety of the experiment. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Craniotomy Basic Protocol 2: Virus injection Basic Protocol 3: GRIN lens implantation Basic Protocol 4: Baseplate mounting and assessment of the anesthetized rat Basic Protocol 5: Assessment of the awake, behaving rat Support Protocol 1: Protective miniscope cone fabrication Support Protocol 2: Miniscope cable fabrication.

Standard requirements for clinical very high energy electron and ultra high dose rate medical devices

Very High-Energy Electrons (VHEE) present a promising innovation in radiation therapy (RT), particularly for the treatment of deep-seated tumors using Ultra High Dose Rate (UHDR) within the framework of FLASH-RT. VHEE offers significant advantages, such as improved tumor targeting, reduced treatment times, and potential utilization of the FLASH effect, which may minimize normal tissue toxicity. However, the lack of an international technical standard for VHEE systems, especially for UHDR applications, remains a critical challenge. Current standards for radiation therapy equipment, such as IEC 60601-2-1 and IEC 60601-2-64, do not encompass VHEE technology. This regulatory gap underscores the need for developing a structured international standard to ensure the basic safety and essential performance of VHEE medical devices. Addressing this challenge requires overcoming complex dose delivery issues, such as the interaction of multiple fields and beam conformality and incorporating novel techniques like broad beam or pencil beam scanning. Establishing comprehensive regulatory standards is essential to ensure patient safety, consistent treatment practices, and the successful clinical integration of VHEE systems. These standards must encompass design guidelines, radiation protection protocols, and integration with existing oncology practices. Collaborative research and development efforts are crucial to formulating evidence-based guidelines, fostering the safe and effective use of VHEE in clinical settings. By addressing these challenges, VHEE technology has the potential to revolutionize cancer therapy, particularly for deep-seated tumors, while enhancing therapeutic outcomes for patients.

India’s Efforts towards Biodiversity Conservation: Ratifying International Commitments as a Way to Bridge the North-South Divide

Since ancient times, India’s rich biodiversity has been a feather in its cap. Thus, housing a rich and diverse ecosystem, India has placed biodiversity as the foundation of its climate politics. Being a signatory to many international conventions and protocols for wildlife protection, many laws, missions, projects and initiatives have been undertaken both on national and international levels for the preservation of rich biodiversity of India. The recent legislations have been proposed by the government in accordance with international agreements on biodiversity conservation to which India is a partner. Also, as a highly populated tropical nation, India runs the risk of protecting biodiversity because of change in land use pattern and other anthropocentric activities. The paper answers a popular question: How far has India’s ratification of international agreements towards biodiversity conservation bridged the North-South divide? Henceforth, the central argument of the paper is to analyse the two ways by which India sets an example of bridging the highly debated North-South divide: by insisting on financing the GS’ (Global South) biodiversity conservation initiatives at the international level while at the national level, promoting tourism, traditional medicine and preserving the habitats of the indigenous communities which are essential for a GS nation inhabited by diverse communities living near the dense tropical forests.

Molecular imaging in experimental pulmonary fibrosis reveals that nintedanib unexpectedly modulates CCR2 immune cell infiltration

Pulmonary fibrosis is a challenging clinical problem with lung pathology featuring immune cell infiltrates, fibroblast expansion, and matrix deposition. Molecular analysis of diseased lungs and preclinical models have uncovered C-C chemokine receptor type 2 (CCR2)+monocyte egress from the bone marrow into the lung, where they acquire profibrotic activities. Current drug treatment is focused on fibroblast activity. Alternatively, therapeutic targeting and monitoring CCR2+ cells may be an effective patient management strategy. Inhibition of CCR2+ cells and, as a benchmark, the clinical antifibrotic agent, nintedanib, were used in mouse lung fibrosis models. Lungs were evaluated directly for CCR2+ cell infiltration and by non-invasive CCR2+ positron emission tomography imaging (CCR2-PET). Lung CCR2+ cells were significantly elevated in the bleomycin model as determined by tissue evaluation and CCR2-PET imaging. A protective treatment protocol with an oral CCR2 inhibitor was compared to oral nintedanib. While we expected disparate effects on CCR2+ cells, each drug similarly decreased lung CCR2+ cells and fibrosis. Chemotaxis assays showed nintedanib indirectly inhibited C-C motif chemokine 2 (CCL2)-mediated migration of CCR2+ cells. Even delayed therapeutic administration of nintedanib in bleomycin and the silicosis progressive fibrosis models decreased the accumulation of CCR2+ lung cells. In these treatments early CCR2-PET imaging predicted the later development of fibrosis. The inhibition of CCR2+ cell egress is likely a critical controller for stabilising lung fibrosis, as provided by nintedanib. Imaging with CCR2-PET may be useful to monitor nintedanib treatment responses, guide decision-making in the treatment of patients with progressive pulmonary fibrosis, and as a biomarker for drug development. National Institutes of Health (NIH), R01HL131908 (SLB), R35HL145212 (YL), P41EB025815 (YL), K01DK133670 (DHK); Barnes Jewish Hospital Foundation (SLB).

Under pressure: A systematic review of the mental health impact of COVID-19 pandemic on mental health workers.

The COVID-19 pandemic emerged suddenly, profoundly impacting the lives of us all, including mental health workers (MHW). This unprecedented crisis introduced significant challenges for MHW, exposing them to increased risks of psychological distress. This systematic review aims to evaluate the psychopathological effects of the COVID-19 pandemic on MHW. Additionally, it seeks to explore factors-social, regional, occupational, and others-influencing these effects. A systematic search was conducted across PubMed, PsycINFO, and Embase databases following PRISMA guidelines. A total of 2621 citations were screened, with 29 studies meeting the inclusion criteria for analysis. Our review focused exclusively on studies utilizing validated measurement tools to ensure reliability. Our findings revealed a high prevalence of anxiety, depression, stress, trauma-related disorders, and burnout among mental health nurses and other MHW during the COVID-19 pandemic, with significant variations based on regional, occupational, gender, and other demographic factors. Depressive symptoms ranged from 16.8% to 52.2%, and anxiety levels varied from 9.7% to 63% among MHW. Interestingly, MHW exhibited lower rates of depression and anxiety compared to other healthcare workers and the general population, possibly indicating higher resilience. Factors such as younger age, female gender, profession, work setting, fear of COVID-19, and workload were associated with increased psychological distress. Our review also underscores the need for more systematically accurate trauma research, particularly in how trauma is defined and assessed during global crises. While the consistency in study findings highlights the considerable effect of the pandemic, we observed differences that suggest the influence of multiple interacting factors. The lack of longitudinal studies and comparative data limits the ability to determine changes over time and differences with other groups. The COVID-19 pandemic significantly affected the mental health of those responsible for caring for the mental health of others, with considerable variations influenced by multiple interacting factors. Our findings highlight the critical need for protective protocols and psychological support systems to mitigate adverse effects on MHW during global crises. The variance in impact across different countries, in relation to local, political, cultural, and other factors, provides a foundation for future research.

International legislative outlets and protection protocols to curb violent and hate speech in digital spaces: balancing technical factors and political influence

International legislative outlets and protection protocols to curb violent and hate speech in digital spaces: balancing technical factors and political influence

Uma Proposta de Atuação em Acidentes Nucleares

This article examines the effects of nuclear accidents, highlighting Chernobyl and Goiânia as examples of tragedies that crossed national borders and impacted public perceptions regarding the safety of radioactive materials. The author emphasizes the need for adequate protection protocols to minimize risks and respond effectively to emergencies. The proposal includes creating specialized medical teams and implementing radioprotection measures within the Brazilian Air Force to ensure a swift and efficient response to nuclear accidents.

Numerical modeling and analysis of neck injury induced by parachute opening shock.

Neck injuries from parachute opening shock (POS) are a concern in skydiving and military operations. This study employs finite element modeling to simulate POS scenarios and assess cervical spine injury risks. Validated against various conditions, including whiplash, the model replicates head/neck kinematics and soft tissue responses. POS simulations capture body/head motions during parachute deployment, indicating minimal risk of severe neck injuries (Abbreviated Injury Score/AIS ≥ 2) and low risk of soft tissue tears. Vertebral stress analysis during a rougher jump highlights high stress at C5/C6 lamina, indicating fracture risk. Comparative analysis with rear impact scenarios reveals distinct strain patterns, with rear impacts showing higher ligament strain, consistent with higher soft tissue damage risk. Though POS simulations exhibit lower strain values, they emphasize similar neck deformation patterns. The model's capability to accurately simulate head and neck movements during parachute openings provides critical validation for its use in assessing injury risks. The study's findings underline the importance of considering specific loading conditions in injury assessments and contribute to refining safety standards for skydiving and military operations. By highlighting the differences in injury mechanisms between POS and rear impacts, this research offers valuable insights into tailored injury mitigation strategies. The results not only enhance our understanding of neck injury mechanisms but also inform the development of protective gear and safety protocols, ultimately aiding in injury prevention for skydivers and military personnel.

Adversarial Attacks on Pre-trained Deep Learning Models for Encrypted Traffic Analysis

For web security, it’s essential to accurately classify traffic across various web applications to detect malicious activities lurking within network traffic. However, the encryption protocols for privacy protection, such as TLS 1.3 and IPSec, make it difficult to apply traditional traffic classification methods like deep packet inspection (DPI). Recently, the advent of deep learning has significantly advanced the field of encrypted traffic analysis (ETA), outperforming traditional traffic analysis approaches. Notably, pre-trained deep learning based ETA models have demonstrated superior analytical capabilities. However, the security aspects of these deep learning models are often overlooked during the design and development process. In this paper, we conducted adversarial attacks to evaluate the security of pre-trained ETA models. We targeted ET-BERT, a state-of-the-art model demonstrating superior performance, to generate adversarial traffic examples. To carry out the adversarial example generation, we drew inspiration from adversarial attacks on discrete data, such as natural language, defining fluency from a network traffic perspective and proposing a new attack algorithm that can preserve this fluency. Finally, in our experiments, we showed our target model is vulnerable to the proposed adversarial attacks.

LEGAL REGULATION OF DIGITISING AND OUTSOURCING MEDICAL RECORDS DEPARTMENT IN INDIA

The whole deal with digitizing and outsourcing medical records in India is complicated. You've got a bunch of laws, guidelines, and ethical considerations all playing a role in ensuring data privacy, security, and patient confidentiality are on point. As the healthcare sector embraces digital solutions more and more, moving from old-fashioned paper records to electronic health records (EHRs) has become a big part of managing healthcare. The Information Technology (IT) Act of 2000, along with its updates, lays the groundwork for all the legal stuff surrounding electronic data and how it's kept secure. Then there's the Personal Data Protection Bill of 2019, which hasn't been passed yet but has some pretty comprehensive measures in place to protect personal data, including health info. On top of all that, the Ministry of Health and Family Welfare (MoHFW) has introduced the National Digital Health Mission (NDHM). This aims to create a national digital health system and puts a lot of emphasis on making sure data privacy and security are top-notch. When it comes to outsourcing the management of medical records to outside parties, there's a need to stick to legal standards and international best practices, like the ones laid out by the Health Insurance Portability and Accountability Act (HIPAA) in the United States. Healthcare providers need to make sure that the folks they outsource to are following confidentiality agreements and data protection protocols. It's all about reducing the risks of data breaches and unauthorized access. The legal framework also requires regular audits, safe data storage solutions, and using fancy encryption technology. To wrap it up, digitizing and outsourcing medical records in India is all about blending existing IT laws, upcoming data protection rules, and specific guidelines for the healthcare industry. The goal is to keep patient data safe while making healthcare more efficient. As the world of digital health data keeps changing, ongoing legal reforms and clear regulatory standards will be important to keep everything in check.

A comparative analysis of remote auditing in Albania: How it affects the quality of enterprise auditing

This paper conducts an extensive examination to explore the impact of remote auditing on audit quality. It aims to ascertain whether remote audit processes can match or exceed the standards set by traditional on-site audits. The study encompasses various remote audit methods, advantages, and challenges. The study employs a mixed methods approach, gathering and analyzing data through both quantitative measures and qualitative techniques. This includes surveys directed at audit professionals to glean insights into remote auditing and its effects on audit quality. Survey findings are analyzed according to a balance scorecard. The findings indicate that remote auditing has the potential to deliver high-quality audits but requires robust technological infrastructure, effective communication channels, and rigorous data protection protocols.

Software as a Medical Device (SaMD) in Digestive Healthcare: Regulatory Challenges and Ethical Implications.

The growing integration of software in healthcare, particularly the rise of standalone software as a medical device (SaMD), is transforming digestive medicine, a field heavily reliant on medical imaging for both diagnosis and therapeutic interventions. This narrative review aims to explore the impact of SaMD on digestive healthcare, focusing on the evolution of these tools and their regulatory and ethical challenges. Our analysis highlights the exponential growth of SaMD in digestive healthcare, driven by the need for precise diagnostic tools and personalized treatment strategies. This rapid advancement, however, necessitates the parallel development of a robust regulatory framework to ensure SaMDs are transparent and deliver universal clinical benefits without the introduction of bias or harm. In addition, the discussion highlights the importance of adherence to the FAIR principles for data management-findability, accessibility, interoperability, and reusability. However, enhanced accessibility and interoperability require rigorous protocols to ensure compliance with data protection guidelines and adequate data security, both of which are crucial for effective integration of SaMDs into clinical workflows. In conclusion, while SaMDs hold significant promise for improving patients' outcomes in digestive medicine, their successful integration into clinical workflow depends on rigorous data protection protocols and clinical validation. Future directions include the need for adequate clinical and real-world studies to demonstrate that these devices are safe and well-suited to healthcare settings.

Pediatric occupant-to-occupant interaction responses in side impact conditions using Q-dummy and child human body models

Objective Road safety of children has improved considerably over the past decade; however, there is still much scope to better protect these occupants. Ensuring adequate protection of children seated in the rear seats requires a greater understanding of how children are injured in side crashes. The purpose of this study was to enhance the knowledge of children’s potential injuries in side impact collisions by the investigation of two different child sizes seated in different positions in the rear seats. The injury metrics associated with the occupant-to-occupant or occupant-to-child restraint system interaction was also identified. Methods Finite Elements Method simulations were performed using a validated simplified vehicle model with fully deformable side components. Dynamic virtual tests according to the Euro NCAP Child Occupant Protection Protocol were performed considering three different scenarios in terms of occupants´ distribution in the rear seats. Simulations were conducted with dummy and human body models. The injury metrics associated with near-side and far-side positions and the occupant-to-occupant interaction were analyzed. Results The 10-year-old child seated in the far-side position presented higher injury values, especially in the head due to the contact with the adjacent child or child restraint occupying the near-side seating position. Similar responses were observed in human body and dummies models in terms of head and neck injury values. However, human body models showed an increase in the chest measurements. Conclusions This study highlighted that the potential injuries of a child seated on the rear seats in a side impact crash are likely to be increased by the presence of an adjacent seat occupant, especially when larger children were seated in the far-side seating location or a CRS was installed in the near-side seating position. Results offer insight into the occupant-to-occupant interaction and occupants’ size as relevant factors to improve children safety under side impact conditions.

Research on Network Security and Privacy Protection Technology of Autonomous Vehicle

With the rapid development of information technology and the increasing maturity of intelligent transportation systems, autonomous vehicles, as an important carrier of future travel, are gradually moving from concept to commercial application. However, the highly connected and intelligent nature of autonomous vehicles also makes them face unprecedented cybersecurity and privacy protection challenges. This paper deeply discusses the security threats and privacy disclosure risks of autonomous vehicles in network architecture, data transmission, data processing and other links, and systematically reviews the current technical means and research progress to address these challenges. This paper Outlines the data collection and transmission mechanism of autonomous vehicles, including the collection and processing of sensor data as well as the communication process between vehicles and the cloud, other vehicles and transportation infrastructure, and analyzes in detail the network security threats faced by autonomous vehicles, including remote control attacks, malware attacks, communication hijacking, etc. These threats can lead to serious consequences such as vehicle failure, data breach, or system crash. At the same time, the paper also points out the challenges faced by autonomous vehicles in terms of privacy protection, such as the risk of leakage of sensitive data such as personal location information and driving habits, as well as the risk of data abuse and sharing. In order to address the above challenges, this paper focuses on the technical research progress of cybersecurity and privacy protection of autonomous vehicles. In the aspect of network security, this paper discusses the key technical means such as data encryption, access control, intrusion detection and response system, and emphasizes the importance of security hardware and software in improving the overall security of the system. In terms of privacy protection, this paper proposes strategies such as anonymization and desensitization technology, privacy protection protocols and standards to protect users' privacy from invasion.

Comprehensive review of smart city cybersecurity strategies

With cities rapidly advancing technologically, the rise of smart cities brings innovative solutions to urban challenges. However, this integration of technologies within urban infrastructures introduces new cybersecurity risks. This review explores the cybersecurity issues that smart cities face and discusses strategies to address these threats. Smart cities rely on interconnected networks of devices and systems to enhance efficiency, sustainability, and citizen services. Yet, this connectivity also creates a complex environment that is vulnerable to cyberattacks. A key concern is the wide array of IoT devices deployed across smart city infrastructures, which often lack robust security mechanisms, making them susceptible to exploitation by cybercriminals. The interconnected nature of these systems heightens the potential impact of cyberattacks, posing significant risks to critical infrastructure, public safety, and privacy. Threat actors can exploit vulnerabilities in interconnected systems, disrupting essential services, manipulating data, or even causing physical harm. Since smart cities heavily depend on data-driven decision-making, ensuring data integrity and confidentiality is a top priority. To mitigate these risks, various cybersecurity strategies have been proposed and implemented. These include technical solutions, regulatory frameworks, and collaboration among stakeholders. Technical measures like encryption, authentication mechanisms, intrusion detection systems, and secure software development practices are essential. Additionally, strong access controls and network segmentation can limit the scope of potential attacks. Regulatory initiatives also play a crucial role in improving cybersecurity standards and ensuring compliance among stakeholders. Establishing clear guidelines for data protection, privacy rights, and incident response protocols is vital for safeguarding citizens' interests. Collaborative efforts between government agencies, the private sector, academia, and cybersecurity experts are crucial in sharing information and collectively defending against emerging threats. Securing smart cities requires a comprehensive approach that integrates technical measures, regulatory frameworks, and collaborative efforts to create a resilient and secure urban environment for all citizens. Keywords: Cybersecurity, Smart City, AI, Technology, Security.

Improving Safety and Comfort of Healthcare Workers Caring for Patients With High-Consequence Infectious Diseases in a High-Level Isolation Unit Using Innovative Approaches.

Patients with high-consequence infectious diseases (HCIDs) require high-quality care by specially trained staff in a high-level isolation unit (HLIU) that follows strict infection prevention and control (IPC) measures. Caring for patients with (suspected) HCID is challenging, mainly because of the strict personal protective equipment (PPE) and IPC protocols healthcare workers (HCW) must adhere to for protection. The Radboud University Medical Center, located in Nijmegen, the Netherlands, has been a dedicated HLIU facility since 2008. A newly built HLIU opened in May 2022, and encouraged us to review the existing PPE selection, IPC protocols, and HCID training program to improve safety and comfort for HCWs working in the HLIU. Based on a systematic search through (inter)national HCID PPE guidelines and semistructured interviews with end users, we selected an improved, more comfortable set of PPE. Additionally, we developed a more concise and easier-to-use patient care process flow and implemented a new teaching strategy. The new way of working was tested in October 2022 when the first 2 patients with suspected HCID were admitted to our unit. We used surveys to evaluate the experiences of HCWs involved in this care to further improve the workflow of the unit. When optimizing safety and comfort for HCWs, it is important to consider (inter)national guidelines as well as user preferences. By systematically evaluating recent experiences of patient admission to the HLIU and then adjusting protocols and training, we can ensure that the quality of provided healthcare and the safety of HCWs working in the HLIU remains high.

Design, development, and operation of an ISO class 5 cleanroom for planetary instrumentation and planetary protection protocols

Cleanrooms are controlled environments where the number of airborne particles is reduced to a level defined by an International Organization for Standardization (ISO) standard. These facilities have applications in different fields, such as the electronic, pharmaceutical, and healthcare sectors, and are also necessary for the assembly, testing and handling of space hardware. Cleanrooms are expensive to build and maintain and require the permanent designation of infrastructures and dedicated spaces within a building. Once built, clean rooms cannot be used for any other purpose, as contamination is a significant risk. The restricted access to these facilities limits the process of designing, testing, and calibrating instruments developed by academic institutions, small companies, and space startups. Here we present a Commercial Off-The-Shelf (COTS) procedure for building and maintaining a highly controlled ISO class 5 cleanroom, according to ISO14644 standards. We provide a detailed explanation of how to design, develop and operate a portable, modular, and cost-effective ISO class 5 cleanroom that can be used for the usual workflow of development, integration, test procedures and planetary protection associated with the design of instrumentation for planetary exploration.