Trail running is an emerging discipline with few studies performed in ecological conditions. The aim of this work was to investigate if and how biomechanics differ between more proficient (MP) and less proficient (LP) trail runners. Twenty participants (10 F) were recruited for a 9.1 km trail running time trial wearing inertial sensors. The MP athletes group was composed of the fastest five men and the fastest five women. Group differences in spatiotemporal parameters and leg stiffness were tested with the Mann-Whitney U-test. Group differences in joint angles were tested with statistic parametric mapping. The finish time was 51.1 ± 6.3 min for the MP athletes and 60.0 ± 5.5 min for the LP athletes (p < 0.05). Uphill sections: The MP athletes expressed a tendency to higher speed that was not significant (p > 0.05), achieved by combining higher step frequency and higher step length. They showed a tendency to shorter contact time, lower duty factor and longer flight time that was not significant (p > 0.05) as well as significantly lower knee flexion during the stance phase (p < 0.05). Downhill sections: The MP athletes achieved significantly higher speed (p < 0.05) through higher step length only. They showed significantly higher knee and hip flexion during the swing phase as well as higher trunk rotation and shoulder flexion during the stance phase (p < 0.05). No differences were found with respect to leg stiffness in the uphill or downhill sections (p > 0.05). In the uphill sections, the results suggest lower energy absorption and more favorable net mechanical work at the knee joint for the MP athletes. In the downhill sections, the results suggest that the more efficient motion of the swing leg in the MP athletes could increase momentum in the forward direction and full body center of mass' velocity at toe off, thus optimizing the propulsion phase.
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