The increasing exploitation of Artificial Intelligence (AI) technologies has enabled the design of user interfaces in a way that integrating artificial intelligence capabilities has become crucial in the modern digital landscape. Exploring the main features and best practices for designing user interfaces for Web applications, which effectively support and leverage AI functionalities, is currently one of the relevant topics in this context. This research work discusses the fundamental principles of user interface (UI) design, and the challenges posed by the integration of AI into web applications. It emphasizes the need to strike a balance between the AI advanced capabilities and the users' ability to understand and control the system. Furthermore, the paper highlights the importance of creating intuitive and engaging UI designs that empower users to interact with AI-driven features effortlessly. The study presents a comprehensive analysis of various UI design techniques specifically tailored for AI-enabled web applications user interfaces. Additionally, the paper explores the incorporation of AI-driven recommendation systems, personalized interfaces, and adaptive designs, which dynamically adapt to users' preferences and behavior. To validate the proposed user interface design principles, the study presents a proposal for a guidelines structure that promotes empirical evaluations through user studies and usability testing. Results collected via a survey based on measuring the effectiveness and user satisfaction of AI-enabled Web interfaces. User interfaces in real-life scenarios are presented and provides information on the impact of UI design decisions on user interaction and overall experience. The outcomes of this research work contribute to a deeper understanding of UI design for AI-supported Web applications user interfaces and offer practical guidelines for designers and developers. By embracing the suggested principles, organizations and designers can create Web interfaces that effectively harness the power of AI while prioritizing user-centricity, accessibility, and ethical considerations.