Proportion Of Acetic Acid Research Articles

Feeding meat goats mangosteen (Garcinia mangostana L.) peel rich in condensed tannins, flavonoids, and cinnamic acid improves growth performance and plasma antioxidant activity under tropical conditions

ABSTRACT The aim of this study was to evaluate the growth performance and plasma antioxidant activity of meat goats fed diets containing mangosteen peel powder (MPP; 41.25 g/kg dry matter). Twelve goats (body weight, 23.1 ± 1.49 kg) were fed a total mixed ration for 0–38 days under a severe temperature–humidity index (temperature, 80.96–103.64°F; humidity, 63%). Body weight and samples of feed, rumen fluid, blood, faeces, and urine were analysed. The MPP diet included condensed tannins, flavonoids, and cinnamic acid. Compared to the control group (CTL; no MPP), dietary MPP increased the nutrient digestibility of organic matter and body weight percentage of goats. Total volatile fatty acids, with a higher proportion of acetic acid, were higher in the MPP group; however, pH and NH3-N remained unchanged for both groups. Methane production in the rumen was reduced in the MPP group. Dietary MPP partly improved biochemical indicators such as plasma total protein (PTP) and albumin (ALB), and modulated antioxidant activity by increasing the glutathione peroxidase concentration and 2,2-diphenyl-1-picrylhydrazyl scavenging activity in plasma. These findings indicate that feeding meat goats the proposed MPP diet represents an environmentally friendly strategy for improving growth performance and plasma antioxidant activity under a severe temperature–humidity index.

Synergistic remediation of lead contaminated soil by microbial fuel cell and composite remediation agent

Microbial fuel cell (MFC) could uses microorganisms to convert the solid wastes into electrical energy. Lead contaminated soil was remediated in MFC through internal electric field reduction, where Pb(II) was reduced to Pb produced on the cathode. When the composite remediation agents of MgO, Ca(H2PO4)2 and montmorillonite were added to the soil, Pb can be solidified and adsorbed in the soil, which reduces the toxicity of lead leaching in the soil and makes Pb exist stably in the soil. After synergistic bioremediation, the Pb concentration was decreased by 374.62 mg/kg, whereas Pb in the acetic acid extractable state was mainly transformed into that in reducible state oxidizable state and residue state. Pb removal efficiency of contaminated soil at pH of 5 was the highest of 22.28% after remediation. The Pb removal efficiency at 10 g/kg Pb was 21.02%. The proportion of acetic acid extracted was decreased by 24.26%∼32.36%, 20.54%∼28.69%, and 34.09∼42.78% at the initial lead concentration of 2.5 g/kg, 5 g/kg and 10 g/kg, respectively. The proportion of extractable acetic acid in 10 g/kg Pb contaminated soil was decreased most, converting to oxidizable state (increased by 18.50%∼22.79%) and residue state (increased by 10.57%∼21.10%). This study provides alternative technology for the remediation of Pb contaminated soil in high concentration.

A Novel Method Development and Validation or the Estimation of Meloxicam in Bulk and Dosage Forms by RP – UFLC

Meloxicam is a non-steroidal anti-inflammatory drug and selective inhibitor of COX-2, it is used particularly for the management of rheumatoid arthritis. In this RP-HPLC method chromatographic separation was achieved on Isocratic elution of the mobile phase Acetonitrile: Ammonium acetate: methanol: glacial acetic acid (Proportion of mobile phase-45:40:10:5 %v/v/v/v) with the flow rate of 1 ml/min. Separation was performed on C18 column (150 mm × 4.6 mm inner diameter, 5 μm particle size). The flow rate was 1.0 ml/min and detector wavelength were kept at 363 nm for monitoring the separation. The method was developed and validated. The linearity range was found to be 10 - 50μg/ml. Regression coefficient was found to be 0.995. Precision study showed % RSD values are less than 2% in all selected concentrations. The recovery studies for Meloxicam were found to be in the range of 99.7 – 100.2%. System suitability parameters remain unchanged even if the composition of mobile phase and wavelengths were changed. The proposed method was very simple, precise, accurate and rapid for determination of Meloxicam from pure and its dosage forms.

Effects of Whole Brown Bean and Its Isolated Fiber Fraction on Plasma Lipid Profile, Atherosclerosis, Gut Microbiota, and Microbiota-Dependent Metabolites in Apoe-/- Mice.

The health benefits of bean consumption are widely recognized and are largely attributed to the dietary fiber content. This study investigated and compared the effects of whole brown beans and an isolated bean dietary fiber fraction on the plasma lipid profile, atherosclerotic plaque amount, gut microbiota, and microbiota-dependent metabolites (cecal short-chain fatty acids (SCFAs) and plasma methylamines) in Apoe−/− mice fed high fat diets for 10.5 weeks. The results showed that both whole bean and the isolated fiber fraction had a tendency to lower atherosclerotic plaque amount, but not plasma lipid concentration. The whole bean diet led to a significantly higher diversity of gut microbiota compared with the high fat diet. Both bean diets resulted in a lower Firmicutes/Bacteroidetes ratio, higher relative abundance of unclassified S24-7, Prevotella, Bifidobacterium, and unclassified Clostridiales, and lower abundance of Lactobacillus. Both bean diets resulted in higher formation of all cecal SCFAs (higher proportion of propionic acid and lower proportion of acetic acid) and higher plasma trimethylamine N-oxide concentrations compared with the high fat diet. Whole beans and the isolated fiber fraction exerted similar positive effects on atherosclerotic plaque amount, gut microbiota, and cecal SCFAs in Apoe−/− mice compared with the control diets.

Potassium ferrate pretreatment promotes short chain fatty acids yield and antibiotics reduction in acidogenic fermentation of sewage sludge

During the acidogenic fermentation converting waste activated sludge (WAS) into short-chain fatty acids (SCFA), hydrolysis of complex organic polymers is a limiting step and the transformation of harmful substances (such as antibiotics) during acidogenic fermentation is unknown. In this study, potassium ferrate (K2FeO4) oxidation was used as a pretreatment strategy for WAS acidogenic fermentation to increase the hydrolysis of sludge and destruct the harmful antibiotics. Pretreatment with K2FeO4 can effectively increase the SCFA production during acidogenic fermentation and change the distribution of SCFA components. With the dosage of 0.2 g/g TS, the maximum SCFA yield was 4823 mg COD/L, which is 28.3 times that of the control group; acetic acid accounts for more than 90% of the total SCFA. The higher dosage (0.5 g/g TS) can further increase the proportion of acetic acid, but inhibit the overall performance of SCFA production. Apart from the promotion of hydrolysis and acidogenesis, K2FeO4 pretreatment can also simultaneously oxidizes and degrades part of the antibiotics in the sludge. When the dosage is 0.5 g/g TS, the degradation efficacy of antibiotics is the most significant, and the contents of ofloxacin, azithromycin, and tetracycline in the sludge are reduced by 69%, 42%, and 50%, respectively. In addition, K2FeO4 pretreatment can also promote the release of antibiotics from sludge flocs, which is conducive to the simultaneous degradation of antibiotics in the subsequent biological treatment process.

Effects of wood kraft pulp as a partial replacement for roughage on rumen fermentation and productivity in dairy cows.

In this study, we evaluated the partial replacement of roughage with wood kraft pulp (KP) on rumen fermentation and productivity of dairy cows. Eighteen cows were divided into control and KP groups. The KP group started adaptation to KP 3weeks before calving; after calving, they were fed a total mixed ration for 12 weeks, wherein 18% Timothy hay was replaced with KP. The dry matter intake, body weight, and milk yield and composition were similar in the control and KP groups. The average daily rumen pH was higher with KP feeding, and the average daily ruminal temperature remained lower at 16 days after calving (P < 0.05). The concentration of volatile fatty acids remained unaltered, the molar proportion of acetic acid decreased, and the molar proportion of propionic acid increased, indicating a low acetic acid:propionic acid ratio (P < 0.05). Lipopolysaccharide activity in the rumen fluid was higher in the KP group (P < 0.05); however, the rumen microbiota were unaffected. The digestibility of dry matter and neutral detergent fiber increased 12 weeks after calving, whereas excretion of urinary nitrogen decreased (P < 0.05). Partial replacement of roughage with KP did not suppress rumen fermentation and maintained postpartum productivity.

The effect of season on in vitro degradability and fermentability of feeds in red deers’ (Cervus elaphus L.) rumen fluid

Transition from summer to winter changes red deer digestive tract physiology and digestive processes. The objective of the trial was to determine the effects of season on in vitro apparent (ivADMD) and true dry matter (ivTDMD) digestibility, in vitro gas production parameters and short-chain fatty acid synthesis (SCFA) in red deer hinds of eleven substrates naturally occurring in Slovenia (chestnut fruits, acorns of common and sessile oak, two fresh grasses) and those frequently used in supplemental red deer feeding (two grass hays and two grass silages, apple pomace and sugar beet roots). There were no differences in ivADMD, ivTDMD, determined by incubation of feeds in buffered rumen fluid, as there were no differences in majority of gas production parameters between autumn and winter season. Only the parameter “C” (specific gas production rate) was frequently higher (p &lt; 0.05) in winter season than in autumn season. The amounts of SCFA were similar between two seasons. However, the proportion of acetic acid tended to be higher in winter, while the proportions of propionic and butyric acid tended to be higher in autumn than in winter especially in high fibre feeds. On contrary, in high starch feeds such as oak acorns and chestnut fruits, the proportion of propionic acid was higher (p &lt; 0.05) in winter, while of butyric acid in autumn (p &lt; 0.05). Despite the fact that the number of used substrates (n = 11) and animal rumen fluid donors (n = 6) were small, these results indicate a shift in rumen microbial metabolism between autumn and winter season.

Enhancing the proportion of gluconic acid with a microbial community reconstruction method to improve the taste quality of Kombucha

In this study, a synthetic microbial community (SMC) was used to enhance the proportion of gluconic acid in order to improve the taste quality of Kombucha. The sensory properties of gluconic acid were analyzed for the first time. The proportions of gluconic acid (r = 0.986, p < 0.05) and acetic acid (r = −0.986, p < 0.05) were significantly correlated with the taste score of Kombucha, which was confirmed by the analysis of the sensory properties of organic acid monomers. Starmerella davenportii with the highest yield of ethanol and Gluconacetobacter intermedius with the highest yield of acetic acid were screened and used for microbial community reconstruction to obtain Kombucha with the highest proportion of gluconic acid (74%). In addition, the sensory quality and concentrations of the main functional metabolites in Kombucha fermented by SMC were significantly increased compared with the traditional Kombucha. The microbial community reconstruction method effectively improved the taste quality of Kombucha.

Response of VFAs and microbial interspecific interaction to primary sludge fermentation temperature

It's not clear how fermentation affects the succession and interspecific interaction of functional microorganism in primary sludge anaerobic fermentation system. So as to explore the decisive role of temperature on microorganisms to reveal the mechanism, four fermentation experiments groups (25, 35, 45, and 55 °C) were designed. Results indicated the promotion in proportion of acetic and iso-valeric acid with temperature increasing. High-throughput sequencing reflected that the dominant microbial groups in all samples were Proteobacteria, Firmicutes, Bacteroidetes, Synergetes, Spirochaetae, and then key microbial interspecific interaction changed significantly due to the adjustment of microbial temperature. In addition, amino acid metabolism was the lowest at 35 °C, but carbohydrate metabolism was the highest. The metabolic abundances of amino acids at different temperatures were the highest, but the differences among kind of amino acids were significant. This study could reveal the effect of temperature on VFAs production in terms of microbial interspecific interaction and lay a theoretical foundation for sludge fermentation.

Production of Organic Acid and Short-Chain Fatty Acids (SCFA) from Lactic Acid Bacteria Isolate on Oligosaccharide Media

The growth of microorganisms in food, one of which is lactic acid bacteria (LAB), can produce metabolites beneficial to health. It is essential to study the results of LAB metabolism to improve the quality of a functional food product. This study aimed to evaluate the isolates Lactobacillus acidophilus FNCC 0051 and Lactobacillus rhamnosus R23 to metabolize oligosaccharides as a carbon source so that the final fermentation product can benefit health especially in lowering cholesterol. In vitro testing was carried out on MRS media with or without oligosaccharides, either singly or in a combination consisting of galactooligosaccharides (GOS), fructooligosaccharides (FOS), inulin (IN), inulin hydrolyzate (HI), or their combination as prebiotics by adding 0.3 % oxbile (bile salt) and inoculated with 1% v/v LAB isolate culture and incubated at 37°C for 24 hours. The results showed that the main product of oligosaccharide metabolism by L. acidophilus FNCC 0051 and L. rhamnosus R23 produced several organic acids (lactic acid), including short-chain fatty acids (SCFA) (acetic acid, propionic acid, and butyric acid). The single and combined carbon sources affected the proportion of lactic acid and acetic acid produced by L. acidophilus FNCC0051 (p&lt;0.05). However, they did not affect the proportions of propionic acid and butyric acid. While in L. rhamnosus R23 (p&lt;0.05), the presence of a single carbon source significantly affected the proportions of lactic acid, acetic acid, propionic acid, and butyric acid, while the combination of oligosaccharides affected the proportions of lactic acid and butyric acid produced. SCFA is the main product of prebiotic metabolism, but the characteristics of the acid produced have not been identified. The fermentation pattern is thought to be related to molecular weight, chain length, and oligosaccharide structure. Short-chain molecules, such as FOS generally ferment more rapidly than long-chain molecules such as inulin. The results of this study indicate that both isolates can be used as probiotics in the development of symbiotic products with the addition of oligosaccharides, which have a physiological effect in lowering cholesterol levels.

Feeding milk supplemented with Ulva sp., Ascophyllum nodosum, or Saccharina latissima to preweaning dairy calves: Effects on growth, gut microbiota, gut histomorphology, and short-chain fatty acids in digesta

Emerging knowledge shows the importance of preweaning nutrition on programming the gastrointestinal microbiome and development of the gut barrier function. The aim of this study was to assess the effects of supplementing cow milk with either intact dried Ulva sp., Ascophyllum nodosum, or Saccharina latissima on growth performance and several gut health parameters of preweaning dairy calves. Forty male Holstein calves were selected based on birth weight (41 ± 4 kg) and plasma Brix percentage (≥8.7%) at d 2 of life. From d 2 to d 42 of life, the control calves (n = 10) were fed with cow milk (8 L/d) without seaweed supplementation, and the experimental calves were fed with cow milk (8 L/d) supplemented with either Ulva sp. (n = 10), A. nodosum (n = 10), or S. latissima (n = 10) at a concentration of 50 g/8 L of cow milk per day (i.e., 5% on a dry matter basis). Calves were weighed every week, and body weight gain and calf starter intake were monitored weekly. At d 42 ± 3 of life, calves were slaughtered. The organ weights and digesta pH from the reticulorumen, mid- and end small intestine, and mid-colon were recorded. A tissue sample (5 cm) collected from the mid-small intestine was analyzed for histomorphology. Digesta from the mid-small intestine and mid-colon were analyzed for lactobacilli, Escherichia coli, and Enterobacteriaceae, and short-chain fatty acid profile. Weight gain of the calves was not affected by seaweed supplementation. Proportional organ weights were not affected by seaweed supplementation except for reticulorumen weight, which was higher in calves fed Ulva sp. Both the mid-small intestinal and mid-colonic digesta populations of lactobacilli, Enterobacteriaceae, and E. coli, as well as the mid-small intestinal histomorphology in seaweed-supplemented calves were not different from control calves. However, acetic acid proportion in mid-colonic digesta was increased in calves fed Ulva sp. and A. nodosum, whereas butyric acid proportion was decreased compared with the control calves. Digesta pH in mid- and end small intestine and mid-colon were not affected, whereas ruminal pH was increased in calves fed Ulva sp. compared with the control calves. In conclusion, intact dried seaweed supplementation did not improve the growth or selected gut health parameters (i.e., histomorphology, digesta pH, bacteria, and short-chain fatty acids) in preweaning Holstein calves.

Sewage denitrification performance and sludge properties variation with the addition of liquid from perishable organic anaerobic fermentation

The organics in the classified wet waste were deficiently utilized while sewage denitrification requires abundant carbon sources. Herein, the fermentation of perishable organic waste (POW) and the denitrification process with obtained liquid were investigated. The most volatile fatty acids (VFAs) production was realized with the fermentation liquid of food waste (FL-FW). Increasing substrate tended to lower the proportion of VFAs and acetic acid in FL-FW. Under the optimum conditions of FL-FW carbon source, carbon to nitrogen ratio 7, and temperature 30 ℃, the removal efficiency of nitrate nitrogen reached 99.23% within 4 h. The sludge settleability and microbial activity were significantly enhanced, contributing to the actual sewage a promotional removal of organics (95.84%) and nitrogen (70.31%) with the supplementation of FL-FW. High addition ratio would cause more degradation of refractory organics, which confirmed the feasibility of using FL-FW as a cost-efficient carbon source for the denitrification.

Study of aroma compound formations and transformations during Jinxuan and Qingxin oolong tea processing

SummaryWe characterised the formation and dynamic changes in the main aroma compounds produced during the manufacturing process of Jinxuan and Qingxin oolong tea. Thirty‐five aroma compounds were investigated. Subsequent principal component and cluster analyses showed that the fresh leaves, spread leaves and rocked leaves of the two varieties of tea were distinguished from each other. Particularly, the aldehyde and ‘other’ compounds showed the highest correlation coefficients (0.71 and −0.70) among the principal. The heat map showed that the proportions of acetic acid, 3‐methylbutanal and 2‐methylbutanal significantly changed during the manufacturing process of the two tea varieties. The key amino acids (isoleucine, leucine, valine and aspartic acid) and enzymes (branched‐chain amino acid aminotransferase, aspartate aminotransferase and acetaldehyde dehydrogenase) involved in the synthesis of 3‐methylbutanal and 2‐methylbutanal differed between the two varieties during manufacture. Our study reveals the characteristics of different varieties of oolong tea and their aroma formation after manufacture using the same process.

КАЧЕСТВО СЕНАЖА ИЗ ЛЮЦЕРНЫ, ПРИГОТОВЛЕННОГО С ИСПОЛЬЗОВАНИЕМ БИОКОНСЕРВАНТА «ГРИНГРАС 3×3»

The aim of the research is to improve the quality and nutritional value of the alfalfa haylage applying the «Green-grass 3×3» biopreservative with herbage. Current biological preparations contain live cultures of specially selected lactic, propionic acids, and sporogenous bacillus, a complex of amino acids, enzymes, vitamins and minor nutrient elements. Preparation use with herbage during haylage leads to intensification the lactic acid fermentation, im-provement of ratio of organic acids, increase the content of nutrients and ensuring their reliable safety during stor-age. The research was carried out at the milk production complex of «Radna» LLC in the Samara region. The re-search was provided involving Holstein and Ayrshire breeds. The haylage was fed to cows in the amount of 24 kg as part of a balanced feed mixture in accordance with the casein diet. As a result of applying a bio-preservative with herbage, the content of organic acids in the ready made feeds increased by 0.54%, including lactic acid – by 0.80%. The dry weight increased by 4.6%, available energy – by 37.4%, energy feed units – by 9.1%, digestible protein – by 13.8%, crude fiber – by 0.9%. Feeding the cows haylage with a bioconservative increased the meta-bolic processes in the tripe of animals. Ciliata weight in the ruminal fluid of Holstein cows increased by 29.0%, Ayr-shire – by 25.8%, number of bacteria, respectively, by 26.5 and 27.5%. As a result, the content of total nitrogen in-creased by 15.0-15.1%, protein nitrogen – by 40.4-42.9%, and ammonia, on the contrary, decreased by 40.0-40.6%. The content of volatile fatty acids decreased by 12.6-16.2%, while the proportion of acetic acid increased by 7.73-8.71%. All this had a positive effect on the digestibility of nutrients by the animals of the experimental groups. The digestibility of the dry weight of the feed increased, respectively, by 4.14-4.57%, and its components – by 4.23-6.97%.

44 Effects of Decreasing Corn Particle Size on Metabolizable Energy and Proportions of Fecal Volatile Fatty Acids in Gestating Sows

Abstract The objective of the first experiment was to determine the effects of corn particle size on the diet apparent total tract digestibility (ATTD) of protein (CP), digestible energy (DE), metabolizable energy (ME) and N-corrected ME (AMEn) in addition to estimating the ME of corn in gestating sows. A total of 27 sows, during the second phase of gestation, were fed a common diet manufactured with corn ground to one of 3 target particle sizes (dgw): 400, 800, or 1200 µm. Titanium dioxide (0.25%) was included in the diet as an indigestible marker for digestibility calculations. Sows were fed experimental diets for 7 days to allow for diet adaptation before a 2-day collection period of urine and fecal samples. Reducing dgw of corn from 1,200 to 400 µm increased (linear, P &amp;lt; 0.01) ATTD CP and GE, DE, ME, AMEn and calculated ME of corn. The objective of the second experiment was to determine the effects of corn particle size on the total concentration and molar proportions of volatile fatty acids (VFA) in the feces of gestating sows. A total of 27 sows were fed treatments similar to Exp. 1 from day 61 to 82 of gestation. On day-82 and 83 of gestation, 2 fecal grab samples were collected for VFA analyses. Sows fed diets with decreasing corn dgw had increased (quadratic, P = 0.021) fecal acetic acid proportions, and decreased propionic (quadratic, P = 0.019) and valeric acid (P = 0.005). In conclusion, for every 100 µm decrease in corn dgw from 1,200 to 400 µm, corn ME value increased by 28.6 kcal/kg. Additionally, decreasing corn particle size led to an increase in the proportion of acetic acid and a decrease in propionic and valeric acid in fecal samples of gestating sows.

Development and validation of a new method for determination of methylparaben in Iran market infant formulae by HPLC.

Parabens are esters of p-hydroxybenzoic acid and are widely used as preservatives in cosmetics, pharmaceuticals and foodstuffs. The presences of parabens in infant formulas raise concerns due to their potential to disrupt endocrine function in infants and cause reproductive toxicities. In this study a new method was developed for extraction and determination of methylparaben in infant formulas using HPLC method and UV detector. Methanol and trichloroacetic acid were used for extraction and isocratic mobile phase comprising equal proportions of glacial acetic acid in water (50:850 v/v) and methanol was used for separation of methylparaben. Recovery of the extraction procedure was good and interferences between methylparaben and other ingredients peaks in HPLC chromatograms decreased. The average recoveries for methylparaben were about 88-108 %. The limit of detection and limit of quantitation for methylparaben were 0.2 and 0.5µg/mL, respectively. Results of the method showed good reproducibility (relative standard deviation (RSD) 0.29-1.94 % for within day analysis and 0.84-2.18 % for between day analysis). Results were linear in range of 0.5-20µg/mL methylparaben. The results of twenty real infant formula samples showed methylparaben was found only in one sample in concentration 0.3µg/mL. The new extraction and measurement method was a short-time method and could be applicable for large numbers of samples. This method was fast, sensitive and accurate and was capable of being used in legal laboratory references for determination of methylparaben content.

Nicotinamide supplementation during postpartum and peripartum modulates hepatic energy and lipid metabolism, oxidative status, and metabolomics profile, as well as lipids in the adipose tissue of goats

Dairy ruminants are often in negative energy balance, primarily in the postpartum period, resulting in many metabolic disorders and economic losses. Improving energy efficiency during the peripartum period is urgently important. It has been previously highlighted that nicotinamide (NAM) supplementation during the peripartum period could benefit energy balance, while the underlying metabolic alterations and differences between postpartum NAM supplementation and peripartum NAM supplementation have not been extensively studied. This study attempted to elucidate the effects of NAM supplementation during the postpartum and peripartum periods on rumen fermentation, liver mitochondrial respiratory chain status, lipid metabolism, oxidative status, and liver metabolite profile of does. Fifteen multiparous does with similarity were paired and allocated to 3 groups (n = 5): control (C, no NAM supplementation), postpartum supplementation (P, NAM supplemented from d 1 to 28 after kidding), and entire-peripartum supplementation (EP, NAM supplemented from d -2 1 to 28 around kidding). Does were drenched with NAM at 5 g/d and subsequently slaughtered on d 28. The ruminal proportion of acetic acid tended to decrease, and propionic acid and valeric acid were increased in P and EP. The does in P and EP had a decreased acetic acid to propionic acid ratio. Does in EP had higher liver mitochondrial respiratory chain complexes. The activity of liver complex Ⅱ was elevated in P and EP, and NAM supplementation tended to increase ATP production. Liver metabolomics and tissue biochemical analyses demonstrated that TG synthesis was decreased in abdominal adipose tissue by NAM supplementation. In the liver, the decomposition and synthesis of TG were both promoted, and amino acid metabolism was enriched by NAM supplementation. Moreover, the oxidative status of the blood and liver was improved by NAM supplementation, and NAM supplementation during the postpartum period was more effective but did not appear to be sufficient to cope with the metabolites of oxidative phosphorylation. Overall, these data suggested that NAM supplementation during postpartum and entire peripartum periods favored the rumen fermentation pattern to propionic acid-type and improved liver energy efficiency with benefited lipid metabolism. Supplementing NAM starting in the prepartum period is necessary for better oxidative status and energy metabolism in the peripartum period.

Effect of Tannin and Flavonoids of Different Sources on Ruminal Fermentation and Microbial Populations

Plant secondary metabolites (PSM|) are used as livestock feed additives instead of chemicals for their multiple effects on rumen microbiota, ammonia N production and fermentation end products. In this study we investigated the effect of two kinds of PSM used as feed additives and compare their effect to feeding whole alfalfa. Fifteen small tail Han rams were randomly allocated into five groups (n= 3/ group). CONT group (basal diet), HT group (basal diet supplemented with 2 g of Hydrolizable tannin), PF group (basal diet supplemented with 2g of Pueraria flavone) G3 group (basal diet +200g of Gannon 3 alfalfa) and G9 group (basal diet +200g of Gannon 9 alfalfa). For 30 days. At the end of the experiment rumen and serum samples were collected for SOD analysis, rumen pH, ammonia N concentration, total and proportional VFA and PCR quantification of total bacteria, F. succinogenase, methanogens and protozoa population. An in situ ruminal digestion kinetics experiment for soybean NDF was applied. Results showed that serum Superoxide dismutase enzyme (SOD), ruminal pH and ruminal ammonia N were not influenced by any of the treatments, but total volatile fatty acids (TVFA) and molar proportion of acetic acid were significantly reduced in HT group. Protozoa and methanogens were significantly increased in G9 and PF groups. Tannins and flavonoids of different of different sources and in different concentrations affect rumen ecosystem differently but, did not affect the serum SOD.

110 Fecal pH, dry matter and volatile fatty acids of horses grazing legume-grass mixed pastures

Abstract Inclusion of legumes into grass pastures improves pasture quality while significantly decreasing the use of nitrogen (N) fertilizer. Changes in nutritive value of the pasture can affect gut microbiome and health. This study aimed to determine the effects of bahiagrass (Paspalum notatum) pasture intercropped with rhizoma peanut (RP, Arachis glabrata) on forage nutrient composition and fecal variables. Unfertilized bahiagrass (UNF) with no N fertilizer, bahiagrass with 120 kg N.ha-1 (FER), and bahiagrass intercropped with RP and 30 kg N.ha-1 (RP) pastures were evaluated using twelve mature Quarter Horses continuously stocked for 84 days in a randomized block design. Forage and fecal samples were collected every 28 days. Pasture samples were analyzed for nutrient composition by NIR, and fecal samples were analyzed for dry matter (DM), fecal fluid pH and volatile fatty acids (VFA). Data were analyzed using a repeated measure mixed model ANOVA and associations between variables were tested using Pearson’s correlations. Pasture starch was higher (P &amp;lt; 0.01) and non-fiber carbohydrates tended to be higher (P &amp;lt; 0.1) in RP. Hemicellulose and aNDF tended (P &amp;lt; 0.1) to be higher in UNF than in RP. Horses grazing RP tended (P &amp;lt; 0.1) to have greater fecal fluid propionic acid concentration and lower proportion of butyric acid. Proportion of acetic acid was lower (P &amp;lt; 0.05) in FER. Propionic acid concentration was negatively correlated with aNDF (r = -0.55) and ADF (r = -0.52), and positively correlated with ethanol-soluble carbohydrates (ESC, r = 0.55). Butyric acid proportion was negatively correlated with nonstructural carbohydrates (r = -0.70). Horses grazing FER pasture tended to have lower (P &amp;lt; 0.1) fecal DM when compared to RP. Fecal DM was negatively correlated with hemicellulose (r = -0.51), and positively correlated with water-soluble carbohydrates (r = 0.68) and ESC (r = 0.64). Intercropping RP provides pasture nutrient composition superior to UNF and similar to FER with decreased N fertilizer rate, and stimulates positive fermentation outcomes.

The effect of the inclusion of pea in lamb fattening concentrate on in vitro and in situ rumen fermentation.

The objective of this study was to evaluate the effect of the proportion of pea (0%, 10%, 20%, and 30%), as a partial replacement for soybean meal (SBM) in the fattening concentrate, on ruminal fermentation in lambs. Gas and methane (CH4 ) production, in vitro dry matter degradability (IVDMD), ammonia (NH3 -N), and volatile fatty acid (VFA) production after 24 h of incubation were evaluated. The concentrates were also incubated in the rumen of the wethers for 2, 4, 6, 8, 12, and 24 h to evaluate the effects of pea inclusion on in situ dry matter degradability (DMD), organic matter degradability (OMD), nitrogen degradability (ND), NH3 -N, and VFA production. In the in vitro assay, the inclusion of pea only affected gas production (mL day-1 degraded dry matter), CH4 production (mL day-1 degraded dry matter), and IVDMD (P < 0.05), and tended to affect NH3 -N content (P < 0.10) without affecting VFA production. In the in situ assay, the inclusion of pea increased DMD, OMD, and ND linearly (P < 0.001), whereas pea inclusion decreased NH3 -N content linearly (P < 0.05). Neither total VFA production nor the proportion of acetic acid were affected by pea inclusion (P > 0.05), but the propionic proportion increased with the proportion of pea included. The best level of pea inclusion in the concentrate could not be established based on the results of this study. However, the results showed that the inclusion of pea provides a good alternative protein source. © 2020 Society of Chemical Industry.