Generation of action potential (AP) in plasma membranes of characean algae has a strong impact on photoreactions occurring in chloroplasts. Under physiological conditions, AP suppresses electron transport in alkaline and acidic regions, although to a different extent; these changes are transient and reversible. In the presence of the artificial electron acceptor, methyl viologen (MV2+), AP-induced changes in electron transport in photosystem II become irreversible. Incubation of Chara corallina internodal cells with MV2+ has no effect on the chlorophyll P700 photooxidation kinetics in photosystem I reaction centers, suggesting that MV2+ is inaccessible for interactions with photosystem I, because its permeation into chloroplasts of a resting cell is hindered by membrane barriers. At the same time, AP generation in the presence of MV2+ is accompanied by irreversible modification of P700 photooxidation kinetics, as can be evidenced from differences in absorption changes at 810 and 870 nm (ΔA810 signals). These findings suggest that MV2+ permeation into chloroplasts in situ is facilitated during or after the AP generation. Similar to the ΔA810 signals, light-induced changes in membrane potential do not depend on the presence of MV2+ in the external medium until the first excitatory stimulus is applied. Electric photoresponses of the cell are irreversibly modified by AP generated in the presence of MV2+ at the expense of non-cyclic photosynthetic electron transport redirected to the MV2+ reduction. It is concluded that AP effects on chloroplast photosynthesis in situ are complex and involve permeability changes for MV2+ in membrane barriers of the “plasmalemma-chloroplast envelope” system.
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