Production Test Research Articles

Experimental production and efficacy testing of mono-specific antibodies against the venom of carpet viper (Echis ocellatus) from savannah Nigeria

Echis ocellatus is one of the commonest snakes responsible for envenomation in Nigeria. Antivenom is the only effective treatment, but the country suffers from a limited supply of effective antivenom. This study therefore aimed to explore the feasibility of effective, mono-specific antibodies production through immunization in rabbits using the venom of Echis ocellatus from Nigeria. The World Health Organization guide on antivenom production was employed in the immunization and the resultant antibodies were purified using protein A agarose column chromatography. Antibody titer reached a high plateau by 2-month immunization, and SDS PAGE of the sera suggests the presence of intact immunoglobulins accompanied with the heavy (50 kDa) and light (25 kDa) chains. The venom has an intravenous LD50 of 0.35 mg/kg in mice, and the venom lethality at a challenge dose of 2 LD50 was effectively neutralized by the antibodies with a potency value of 0.83 mg venom per g antibodies. The antibodies also neutralized the procoagulant activity of the venom with an effective dose (ED) of 13 ± 0.66 μl, supporting its use for hemotoxic envenomation. The study establishes the feasibility of developing effective, mono-specific antibodies against the Nigerian Carpet viper.

UVLHub: A feature model data repository using UVL and open science principles

Feature models are the de facto standard for modelling variabilities and commonalities in features and relationships in software product lines. They are the base artefacts in many engineering activities, such as product configuration, derivation, or testing. Concrete models in different domains exist; however, many are in private or sparse repositories or belong to discontinued projects. The dispersion of knowledge of feature models hinders the study and reuse of these artefacts in different studies. The Universal Variability Language (UVL) is a community effort textual feature model language that promotes a common way of serializing feature models independently of concrete tools. Open science principles promote transparency, accessibility, and collaboration in scientific research. Although some attempts exist to promote feature model sharing, the existing solutions lack open science principles by design. In addition, existing and public feature models are described using formats not always supported by current tools. This paper presents ▪ , a repository of feature models in UVL format. ▪ provides a front end that facilitates the search, upload, storage, and management of feature model datasets, improving the capabilities of discontinued proposals. Furthermore, the tool communicates with Zenodo – one of the most well-known open science repositories – providing a permanent save of datasets and following open science principles. ▪ includes existing datasets and is readily available to include new data and functionalities in the future. It is maintained by three active universities in variability modelling.

Experimental study and prediction method of solid destabilization and production in deep carbonate reservoir during mining

Wellbore instability is one of the significant challenges in the drilling engineering and during the development of carbonate reservoirs, especially with open-hole completion. The problems of wellbore instability such as downhole collapse and silt deposit in the fractured carbonate reservoir of Tarim Basin (Ordovician) are severe. Solid destabilization and production (SDP) was proposed to describe this engineering problem of carbonate reservoirs. To clarify the mechanism and mitigate potential borehole instability problems, we conducted particle size distribution (PSD) analysis, X-ray diffraction (XRD) analysis, triaxial compression tests, and micro-scale sand production tests based on data analysis. We found that the rock fragments and silt in the wellbore came from two sources: one from the wellbore collapse in the upper unplugged layers and the other from the production of sand particles carried by the fluid in the productive layers. Based on the experimental study, a novel method combining a geomechanical model and microscopic sand production model was proposed to predict wellbore instability and analyze its influencing factors. The critical condition and failure zone predicted by the prediction model fit well with the field observations. According to the prediction results, the management and prevention measures of wellbore instability in carbonate reservoirs were proposed. It is suggested to optimize the well track in new drilling wells while upgrading the production system in old wells. This study is of great guiding significance for the optimization of carbonate solid control and it improves the understanding of the sand production problems in carbonate reservoirs.

Investigation of five flow channel designs in PEM electrolysis with titanium felt

This study investigates titanium felt (TF) as a flow diffuser in proton exchange membrane water electrolysis (PEMWE) systems, addressing challenges related to the oxygen evolution reaction (OER). Made from sintered titanium fibers, TF offers conductivity, corrosion resistance, and mechanical strength. Its porous structure improves fluid circulation and reaction surfaces, thereby reducing the risk of membrane damage. Utilizing FR4 plates to create five different types of flow plates, combined with TF, forms a single electrolysis cell. After assembling with current collectors, gaskets, and membrane electrode assemblies, hydrogen production tests are conducted. Variables include voltage, water temperature, and flow rate to assess efficiency and hydrogen production. Results demonstrate that parallel flow channels achieve higher hydrogen production and efficiency across different temperatures compared to four other channel types.

Pengembangan Media Papan Sosial Emosional (Pansos) untuk Meningkatkan Perkembangan Sosial Emosional Anak Usia 5-6 Tahun

Lilia Fitriyati (NIM: 214223046), Development of Social-emotional Board Media (Pansos) to Improve Social Emotional of 5-6 Years Old Children (PGPAUD), 2023.Early childhood is a golden age, a time when growth and development begin and are on going. One aspect of child development that needs to be developed by educators is the aspect of social development. Social development aspects can be developed with game media. Educators should use interesting media in their learning. One way to develop social-emotional aspects is with Social-emotional Board Media (Pansos).Adopting from the monopoly game This study aims to determine the social-emotional development of children before and after using Social-emotional Board Media (Pansos) for children aged 5-6 years at Kober Nur Ismail. The purpose of this research and development is to find out the increase in children's social-emotional abilities through the use of learning media Social-emotional Board (Pansos). This type of research is research and development by adopting Sugiyono's steps. The research method used in this research is Research and Development (R&D) using steps from Borg and Gall which are simplified into stages, namely potential and problems, data collection, product design, design validation, design revision, product testing. The subjects of this study were children at Kober Nur Ismail who were 5-6 years old. From the results of the study it was known that there was an increase in social emotional development by 42.5% with the results of the initial presentation during the pre-test 40% and post-test 82.5%

The Integration Hydroponic Aquaculture Systems For Optimizing Catfish Growth Management with Arduino

Modern agriculture faces significant challenges such as limited land and declining soil fertility. The integration of hydroponic systems with aquaculture, particularly catfish farming, offers an innovative solution to enhance agricultural efficiency and productivity. This study explores the integration of hydroponic systems with aquaculture for the simultaneous cultivation of plants and catfish in a controlled environment. The research utilizes Arduino technology for monitoring temperature, pH, and water turbidity, which are essential for the health of fish and plants. The research method used is Research and Development (R&D), involving the following steps: (1) Identifying potential problems, (2) Data collection, (3) Product design, (4) Design validation, (5) Design revision, and (6) Product testing. The results indicate that integrating hydroponic systems with Arduino technology improves the efficiency of monitoring and managing the cultivation environment, positively impacting plant and catfish growth. The implementation of this system shows an increase in plant nutrient content and better fish health. And then, this research significantly contributes to the development of sustainable agriculture and global food security through the adoption of innovative technology.

The Development of Android-Based PowerPoint Learning Media on Wave Material to Determine the Learning Interests of Class XI High School Students

Technological advancements in education have enabled the development of more interactive and easily accessible learning media for students. One such innovation is the use of an Android-based PowerPoint application, which can be utilized both offline and online to enhance students' interest and engagement in learning. This study aims to evaluate the feasibility of using an Android-based PowerPoint application, measure the responses of students at SMAN 1 Lenek to the application, and assess the differences in students' learning interest before and after using this learning media. The research employs a Research and Development (R&D) methodology, following six procedural stages: problem potential analysis, observation and assessment, design and development, product testing, revision, and implementation. Data were analyzed using the ideal standard deviation method and normalized gain. The ideal standard deviation method was employed to assess the validity of the instruments evaluated by media and material experts, as well as to measure the students' responses to the application. Normalized gain was used to determine the change in learning interest scores from questionnaires distributed before and after the learning process. The results of the study indicate that the developed Android-based PowerPoint application successfully increased the learning interest of 11th-grade science students at SMAN 1 Lenek, with a gain score of 0.225, categorized as low. The students' responses to the use of this application were very positive, with an overall average score of 4.4. This research successfully developed an Android-based PowerPoint application that can be accessed both offline and online, contributing positively to the improvement of learning quality.

Utilization Application Mobile for Speed Up Searching Boarding House Android Based

Purwokerto is a city in Central Java Province. Purwokerto's development is currently relatively rapid, both in the economic and educational fields. There are many boarding houses for rent in the Purwokerto area, especially around campuses and business areas. However, the majority of boarding house owners still market their boarding places manually, such as putting up the words 'Accept Boarding House' in front of the house or by hiring a jockey. Along with current technological developments, especially in the smartphone sector, many applications have emerged that help users. To bridge the needs of users and boarding house providers, an application is needed that can display information on boarding houses in Purwokerto. The application was built on a mobile basis with the Android operating system for users who want to find a boarding house. Meanwhile, boarding house providers use a web-based system that can update boarding house advertisements. The method used in preparing this research uses the Waterfall model. The testing stage is carried out by conducting product tests. Product testing is carried out by testing the application using the reliability and attributes durability, conformance, serviceability, apperarance and perceived quality. The Android-based boarding house information system in Purwokerto can help users or boarding house seekers to obtain information about boarding houses more quickly, so the user becomes more efficient in Look for the accommodation you want.

Facile one-pot in-situ hydrothermal preparation of 0D/2D Mn3O4/CdS heterojunctions for improved photocatalytic H2 production and degradation of methylene blue

Developing heterojunction photocatalysts is a critical method to augment the photocatalytic activity of single semiconductors. However, the instability of their heterostructure poses a significant challenge. Herein, a unique 0D/2D Mn3O4/CdS p-n photocatalyst has been prepared by a simple one-pot in-situ hydrothermal approach for H2 production and methylene blue (MB) degradation. The optimized MC-0.18 (18 at% Mn) nanocomposite showed remarkable photocatalytic properties, producing a significantly higher H2 generation rate of 1291 μmol·g−1·h−1, which was 13.7 and 9.2 times higher than that of pristine CdS and ex-situ synthesized MC-0.18-ex photocatalysts, respectively. MC-0.18 displayed the highest reaction rate at 0.0435 min−1 for MB degradation, which was approximately 2.6 and 6.3 times higher than CdS and Mn3O4, respectively. Active species capture experiments and ESR identified •OH and •O2− as the main substances involved in MB degradation. Importantly, MC-0.18 demonstrated excellent stability and reusability in photocatalytic H2 production and MB degradation cycle tests. The formation of unique p-n heterostructures results in enhanced photoactivity due to the matched band alignment between ultrafine Mn3O4 nanoparticles and CdS nanosheets, leading to a high charge separation capacity. This study offers a facile route to design p-n heterojunctions that have remarkable charge transfer abilities to promote H2 production and degrade organic dyes.

Characterization and biocontrol potential of some rhizobacteria against fungal pathogens causing foliar diseases in maize

Maize is one of the most consumed cereal crops worldwide, and it is a strategic crop to the attainment of SDG 2 of Zero hunger. Despite its importance, the cultivation of maize has been significantly impaired by fungal pathogens causing foliar diseases. The occurrence of this disease in maize plantations at the Research Farm of the North-West University, Molelwane, Mafikeng, South Africa prompted this investigation. Samples of diseased maize rhizosphere soil were aseptically collected. Bacteria species associated with the rhizosphere were isolated and characterized as Bacillus siamensis, Enterobacter asburiae, Enterobacter chengduensis, Priestia aryabhattai, Burkholderia sp., Priestia megaterium strain AOA6 and Priestia megaterium strain AOA7. The anti-fungicidal potentials of the bacterial species were evaluated against pathogenic fungal species, Nigrospora sphaerica, Alternaria alternata and Fusarium equiseti in-vitro. The percentage mycelia growths were calculated and the data were subjected to ANOVA using SAS version 9.8. All the seven bacteria isolates tested positive to ammonia production, phosphate solubilization, siderophore production and ACC deaminase tests. The percentage mycelia inhibition showed Nigrospora sphaerica (36.29%), A. alternata (26.19%) and F. equiseti (20.63%) as the order of fungal inhibition by the bacteria species. Furthermore, E. asburiae > P. megatarium strain AOA7 > B. siamensis > P. aryabhattai > E. chengduensis > Bulkholderia sp. were the order of antifungal efficacy of the bacteria species evaluated. In conclusion, the efficacy of the bacteria especially E. asburiae, P. megatarium strain AOA7 and B. siamensis over various fungal pathogens. The result obtained, therefore, justifies the further investigation, formulation and deployment of the bacteria species as biofungicide in the management of foliar diseases of maize. Keywords: antifungicidal potential, biofungicide, microbial formulations, rhizosphere, zero hunger.

Development and Implementation of The Bilingual "Ulos Weaving" Textbook in The Fine Arts Studies Program as Implementation of An Outcomes-Based Curriculum

The aim of research is to develop and apply teaching materials for the Ulos Weaving course in two languages. This is considered important and urgent to address curriculum implementation as per the requirements for ASIIN, ABET and AUN QA accreditation or certification. The problem studied is the validity, effectiveness and practicality of the product being developed. This applied research uses the ADDIE development model developed by Dick and Carry with five stages: analysis, design, development, implementation and evaluation. The objects of product testing were students from the fifth semester fine arts education study program in 1 class (21 people) from 4 classes and 6 lecturers. This research creates innovation and development of teaching materials that are oriented towards textbook products which have an impact on improving the quality of graduates. After the product was validated by 4 experts, material validity was found to be 88% in the very effective category, validation of the layout or appearance of the book was 90%, and validation in Indonesian was 89% and English was 85% in the good category. The results of the student questionnaire were 86.57% and 100% of the lecturers said it was very useful. With the implementation of this textbook, it is hoped that the Ulos Weaving course will become an idol because it is a typical course of the Unimed Fine Arts Department. With this textbook, it will attract the interest of students from other universities and foreign students to study in the FBS Unimed Fine Arts Education Study Program with the Independent Campus Learning Curriculum which is oriented towards results.

Development of A Drama Text Writing Module Based On Character Education for Students Junior High School

This research aims to produce a drama text writing module based on character education for students at SMP Negeri 1 Batang Angkola. The research method used is the Research and Development research method which refers to the Borg and Gall model. The process of developing teaching materials for writing drama texts based on character education is carried out in three stages, namely the preliminary study stage, initial product development and product testing. The results of material expert validation on content suitability obtained an average of 88% in the very good category, the presentation feasibility aspect obtained an average of 84.33% in the very good category and the language assessment obtained an average of 82.5% in the very good category. for graphic assessment by design experts, an average of 80.08% was obtained in the good category. Individual trials obtained an average percentage of 79.77 in the "good" category. Small group trials obtained an average percentage of 85.96 in the "very good" category. Limited field trials obtained an average percentage of 91.87 in the "very good" category. Based on these data, teaching materials that are suitable for teachers and students to use in learning are obtained. Teaching materials for writing drama texts based on character education were declared effective in generating student activity and learning outcomes. This is proven by tests on student learning outcomes in writing drama texts. At the pretest the average was 69 and at the posttest the average was 80. The difference between the pretest and the posttest was 11%, which indicated that learning using teaching materials for writing drama texts based on character education was better than before.

Design and development of solar parabolic concentrator, closed-loop pressurized hot water for a household baking system – For the case of injera baking in Ethiopia

A laboratory setup with two axis of manual sun-tracking was designed and built. To get the predicted values for a solar injera stove, a 20-min heating time to prepare it for baking, 3.1 kWh power in the first hour, and 5.8 kWh (with only an 87 percent increase) in the second hour were acquired to bake 9 and 28 injera using electric injera stove, respectively. The temperature distribution on the clay surface was the range of 180 °C–220 °C. Solar injera baking stove was designed and built using the average energy required to bake one injera from an electrical injera stove. The laboratory setup was put to the test, temperatures at the receiver's surface ranging from 315 to 318 °C and an average solar radiation of 1000 W/m2 was obtained on the first test. The injera stove inlet reached a maximum temperature of 180.92 °C with a pressure of 14.5 bars during a pressurized hot water production and circulation test (load test). The surface temperature of the solar injera stove clay plate reached a maximum of 62.69 °C. Temperature of more than 60 °C was maintained on the surface of the solar injera stove plate for nearly 2 h.

Busworld Website Development To Improve Business Vocabulary Achievement Of Vocational School Marketing Students

This study intends to ascertain the potential, issues, and requirements for learning English for marketing majors at vocational schools. It also seeks to evaluate the feasibility of the Busworld website, which was created as an alternate medium for this research. English communication abilities must be improved by vocational students. Issues that arise with marketing majors at vocational schools show that the same materials are still used in both general education and vocational education. A notable example of technology use in this study is the technology-based learning medium, which took the shape of a website centered around telecommunications. The Sugiyono Model is the model that is adapted for this research, which employs the Research and Development (R&D) technique. Finding Potential, Problems, and Data Analysis, Design Validation, Product Design, Product Testing, Product Revision, Final Product, and Publication are the steps that have been modified. The SMK Pemuda 1 Kesamben, SMK PGRI 2 Sutojayan, and SMKN 1 Kademangan served as the research's field sites. This study's data is quantitative in the form of teacher and student answers and product validation results, and qualitative in the form of observations, interviews, and documentation. The study's findings suggest that using the Busworld website as an alternate medium can help marketing students expand their vocabulary related to business. The results of the media validation and material validation, which were completed by three experts each, and which yielded a score of 98%, indicating high validity, serve as evidence for this. Additionally, 88.5% of the replies from thirty marketing students and three teachers were included in the results of the teacher and student surveys. In summary, the Busworld website, which offers resources such as 100 lists of verbs, 100 lists of nouns, 50 business abbreviations, and 50 conversations with business themes, is a valuable tool for improving the business vocabulary proficiency of marketing students at vocational schools.

Canva-based Smart Apps Creator Media to Enhance Comprehension Skills of Informational Text for Third-Grade Students

Learning media, as a tool for delivering information from teachers to students, aims to facilitate the teaching and learning process in school. Smart Apps Creator is a software with several advantages for developing interactive learning media, one of which is with the assistance of Canva application as its online design application. This research aims to create Canva-based Smart Apps Creator media to enhance the ability to understand the content of information texts in third-grade elementary schools. The research method used is Research and Development (R&D) based on Sugiyono's research with the Borg & Gall development model modified into eight stages, namely: potential and problems, data collection, product design, design validation, design revision, product testing, product revision, and usage testing. This research utilizes data collection techniques through tests and non-tests. The data analysis techniques used include both qualitative and quantitative data analysis. The material expert validation result obtained a percentage of 90%, and the media expert validation obtained a percentage of 92%, both categorized as "highly feasible." Using Canva-based Smart Apps Creator media in learning activities is quite effective through N-gain testing, with students' learning outcomes reaching 0.66 (65.6%), and the average pre-test score increased from 55 to 83 during the learning process (post-test). From these results, the Canva-based Smart Apps Creator media is declared highly suitable for learning and sufficiently effective in improving the ability to comprehend the content of information texts in Indonesian language classes for third-grade elementary school.

Examining the Concurrent Contribution of Receptive and Productive Vocabulary Breadth Aspects to Moroccan EFL Students’ Speaking Ability

The present paper investigates the contribution of productive as well as receptive vocabulary breadths to Moroccan learners’ EFL speaking ability. The study’s significance lies in its exploration of the under-researched area of speaking proficiency, furthering our understanding of the link between vocabulary knowledge and language skills. To further probe this connection, particularly in the high school context, this study investigated the relationship between vocabulary size and speaking ability using valid and reliable vocabulary research instruments. This study addressed two research questions: a) to what extent do receptive and productive vocabulary size scores correlate with speaking ability? b) to what extent do receptive and productive vocabulary size scores explain the variance in learners’ speaking ability? To answer these questions, the researchers administered the Updated Vocabulary Levels Test, the Productive Vocabulary Levels Test, and a speaking task to 40 tenth-grade EFL participants. Spoken data were graded by reference to IELTS’ speaking band descriptors. Results of Pearson Correlation disclosed positive correlations between receptive and productive lexical breadths and speaking proficiency. Moreover, findings of multiple linear regression revealed that receptive and productive vocabulary sizes could explain performance in speaking ability. Implications for EFL vocabulary instruction and speaking proficiency development are discussed.

Development of Electrical Circuit Display Panel for Light Vehicle Turn Signal Lights as a Learning Media for Vocational School Students

This research aims to develop a demonstration panel for the electrical circuit of light vehicle turn signal lights as a learning medium. The purpose of making this demonstration panel is to describe the process of making a demonstration panel for the electrical system of turn signal lights on light vehicles, to describe the feasibility test of the electrical circuit demonstration panel for light vehicle turn signals and to describe students' responses to the electrical system demonstration panel for turn signal lights on light vehicles. This research uses the Research and Development method. The subjects in this research are material experts and media experts as well as students, and the objects are teaching materials and students. The instruments used are: observation, questionnaires and documentation. Research procedures include potential and problems, data collection, product design, design validation, design improvement, and product testing. The data analysis technique used is a descriptive analysis technique by explaining the results of making the product in the form of a learning media display panel, testing the validity and suitability of the product to be implemented in lighting systems and light vehicle instrument panels. 1) The results of the process of making electrical circuit display panels for light vehicle turn signal lights as a learning medium have been successfully described in detail, including the steps and components used. 2) The results of the demonstration panel feasibility test after being validated by material expert I scored 93.75%, and material expert II gave a score of 96.87%. Meanwhile, the feasibility results of the media expert panel I gave a score of 100%, and media expert II gave a score of 95.83%. 3) The results of students' responses to the demonstration panel received a very good response, the overall score obtained was 90.38%, which is very suitable.

Implementasi Metode Mobile D pada E-Posyandu berbasis Android sebagai Alat Literasi dalam Mencegah Stunting Anak Usia Dini

Stunting is a worldwide health problem that impacts children's physical growth and development. According to the latest Indonesian Nutrition Status Survey (SSGI), the stunting rate in Indonesia will reach 21.6% by 2022. Due to its high prevalence, stunting is a major concern. This condition can affect the future of society, the quality of life and the productivity of children. Among the factors causing stunting are inadequate nutrition, unhealthy pregnancies, improper feeding methods, lack of hygiene and sanitation, economic and social factors, infections and chronic diseases, lack of access to health services, and lack of knowledge about the importance of maintaining health for growing children. To solve this problem, an Android-based application is needed that can be accessed anywhere. In this research, the method used is Mobile D consisting of five phases: exploration, Initialization, Production, Stabilization, and System Testing and Fix. It is hoped that the E-Posyandu Application can improve community access to basic health services. With this application, the danger of stunting can be avoided because pregnant women, mothers of toddlers, and other posyandu members can easily access information about child growth and development info, immunization info at each developmental period, info on the location of the nearest RSIA, and complementary food information for toddlers over the age of 6 (six).

СРЕДСТВА ЗАЩИТЫ НА РАБОЧЕМ МЕСТЕ ЛАБОРАНТА ХИМИЧЕСКОГО АНАЛИЗА В ИСПЫТАТЕЛЬНОЙ ЛАБОРАТОРИИ НЕФТЕХИМИЧЕСКОГО ПРЕДПРИЯТИЯ

The article considers protective equipment and the need for their use at the workplace of a chemical analysis laboratory technician performing analyses in a petrochemical production testing laboratory

Protective effect and antioxidative efficacy of Moringa stenopetala leaf extract in BV-2 microglial cell

Moringa stenopetala is often consumed as food and used in folkloric medicine for the management of several diseases including neurodegenerative diseases. This study investigated the protective effect and antioxidant efficacy of aqueous extract of Moringa stenopetala in BV-2 microglial cells. Aqueous extracts of M. stenopetala were prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with M. stenopetala extracts (0.1–100 µg/ml) for cell viability assay and nitric oxide (NO) production tests. However, M. stenopetala extract (50 µg/ml) was used in the treatment of cells for the determination of protein carbonyl content and reactive oxygen species (ROS) level. Incubation of BV-2 microglia cell with M. stenopetala extract maintained cell viability, diminished NO and ROS levels, and reduced protein carbonyl contents. Chlorogenic acid, rutin, kaempferol and quercetin derivatives were the main phenolic compounds identified in M. stenopetala leaf extract. In conclusion, this study suggests that M. stenopetala extract is rich in antioxidant and contains bioactive neuroprotective agents.