Received: 2016-04-11 | Accepted: 2016-05-04 | Available online: 2016-12-22 http://dx.doi.org/10.15414/afz.2016.19.04.163-166 The aim of the study was to analyse the effect of feeding of different sources of NPN on nutrient utilization and production performance of dairy cows under field conditions. Balancing diets for crude protein without consideration of protein quality or rumen degradability often led to overfeeding of nitrogen and less than optimum production. High yielding dairy cows separated in two groups with 85 resp. 80 cows in each were set up for the trial. Groups were consistent according the stage of production and reproduction cycle as well as age structure. Both groups were fed concentrate mixture with the same composition with only difference in NPN/ microbial protein source, with same dosage of 100 g per cow and day. Field trial was performed for period of 3 subsequent months. Performance data were collected in accordance with official milk recording. In both groups majority of cows were on first lactation. Significant differences in daily milk production were observed 2.87 kg (P<0.01) for group 2, in fat content 0.07 % for group 2 non-significant, whereas in protein content 0.18% for group 1 significant (P<0.01) in case of first lactations. If considering first tree lactations, group 2 produced 1.7 kg milk per day more (P<0.08), with 0.05% fat more and 0.002 % protein less than group 1. The space created in dry matter intake by a concentrated slow-release NPN can be filled with high quality forage that could reduce the cost of feeding while maintaining levels of production. Keywords: Holstein, slow-release urea, microbial protein, milk yield References Biro, D., Galik, B., Juracek, M. et al. (2009) Effect of Biological and Biochemical Silage Additives on Final Nutritive, Hygienic and Fermentation Characteristics of Ensiled High Moisture Crimped Corn. Acta Veterinaria Brno , vol. 78 (4), pp. 691-698 doi: http://dx.doi.org/10.2754/avb200978040691 Bouska J. et al. (2006) Chov dojeneho skotu, Profi Press, Praha , 2006 Cantalapiedra-Hijar, G., Peyraud, J. L., Lemosquet, S. et al. (2014) Dietary carbohydrate composition modifies the milk N efficiency in late lactation cows fed low crude protein diets. Animal , vol. 8 (2), pp. 275-285 doi: http://dx.doi.org/10.1017/S1751731113002012 Cappellozza, B. I., Bohnert, D. W., Schauer, C. S. et al. (2013) Daily and alternate day supplementation of urea or soybean meal to ruminants consuming low-quality cool-season forage: II. Effects on ruminal fermentation Livestock Science , vol. 155 (2-3), pp. 214-222 doi: http://dx.doi.org/10.1016/j.livsci.2013.05.002 De Boever, J. L., Blok, M. C., Millet, S. et al. (2014) The energy and protein value of wheat, maize and blend DDGS for cattle and evaluation of prediction methods. Animal , vol. 8(11), pp 1839–1850 doi: http://dx.doi.org/10.1017/S1751731114001815 Harrison, G. A. and Karnezos T. P. (2005) Can we improve efficiency of nitrogen utilization in the lactating cow? Recent Advances in Animal Nutrition , vol. 15, 2005, 001-011pp. Hazuchova E. and Kasarda R. (2010) Evaluation of body condition score of lactating cows. 61st EAAP Annual Meeting, Heraklion. 2010 . Book of Abstracts. 34, 26,p. 375 Holder Vaughn B., El-Kadi, Samer W., Tricarico, Juan M. et al. (2013) The effects of crude protein concentration and slow release urea on nitrogen metabolism in Holstein steers. Archives of Animal Nutrition , vol. 67 (2), pp. 93-100 doi: http://dx.doi.org/10.1080/1745039X.2013.773647 Kudrna V. and Homolka P. (2009) Vliv diety, zejmena obsahu dusikatých latek, na množstvi a kvalitu mlecne bilkoviny a zdravi dojnic, Výskumný ustav živocisne výroby, Praha – Uhřiněves, 2009 McGuire D. L., Bohnert, D. W., Schauer, C. S. et al. (2013) Daily and alternate day supplementation of urea or soybean meal to ruminants consuming low-quality cool-season forage: I-Effects on efficiency of nitrogen use and nutrient digestion Livestock Science , vol. 155, (2-3), pp. 205-213 doi: http://dx.doi.org/10.1016/j.livsci.2013.05.015 Simko, M., Ceresňakova, Z. Biro, D. et al. (2010) Influence of Wheat and Maize Starch on fermentation in the Rumen, Duodenal Nutrient Flow and Nutrient Digestibility. ActaVeterinaria Brno , vol. 79 (4), pp. 533-541 doi: http://dx.doi.org/10.2754/avb201079040533 Zeman, L. et al.(2006) Výživa a krmeni hospodařských zviřat., Profi Press, Praha , 2006
Read full abstract