Using soil bed cultivations as controls and under two temperature regimes (maximum/minimum ≈20/4 °C and 25/10 °C), effects of container volume (16-, 10-, and 3-L pots) on in vitro germination, in vivo tube growth, acetocarmine staining, and quantity of pollen of tomato [Lycopersicon esculentum Mill. `Moneymaker', L. pennellii (Corr.) D'Arcy accession PE-45, and of the corresponding F1 hybrid] were studied. Under the 20/4 °C regime, in comparison with soil-bed cultivated control plants, the cold sensitive cultivar, Moneymaker, grown in the two smaller pots showed significant increases of in vitro pollen germination, acetocarmine staining, and number of pollen grains produced per flower. Similar results were observed with the F1 except for the number of pollen grains which were not significantly different. Pollen of accession PE-45 was unaffected by cold and no container effect was detected. Results of in vivo pollen tube growth in `Moneymaker' at the 20/4 °C regime showed that fruit set was only possible in 3-L pots. Reduction of the negative effects of cold on pollen from plants grown in the 3-L pots may be explained in part by the daytime rise of root-zone temperatures that did not occur in the 10- or 16-L pots or in the soil bed. Therefore, fruit production of tomato plants grown under low temperatures in small pots may not be a valid predictor of commercial winter fruit production of plants cultivated in soil beds.
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