AbstractA value chain analysis (VCA) is a cost‐effective tool to guide targeted value chain development interventions to address social wellbeing and environmental performance. Examining value chains through a gender lens can help design and implement interventions that enhance opportunities for women in the fisheries and address gender inequalities in the sector. We conducted a VCA in 2015 of the wild‐caught mud crab (Scylla serrata) fishery in Bua Province, Fiji. We found five main players involved in the selling of mud crabs – fishers, traders, retail shops, restaurants and exporters. The value chain was dominated by Indigenous (iTaukei) women fishers (88.1% of fishers) and characterised by low technological input, targeted largely for domestic markets or consumption, and with limited value‐adding activities. Although most women harvested mud crabs on a part‐time basis, it was an important source of income for most with 30% relying on it as their main livelihood. Despite being a lucrative commodity, there are several bottlenecks in the fishery – the relative informality of relationships amongst players in the value chain, the independent livelihood‐driven harvest behaviours of fishers, and opportunistic sale of products. As a result, the fishery did not meet the demands of the domestic market. Our study concluded the gendered‐skewness in the fishery increases the vulnerability of the chain to declines in economic productivity because of its reliance on irregular suppliers, and gender‐based constraints. However, the low frequency and intensity of harvesting and use of low technological harvesting methods meant the fishery was not over‐exploited and likely sustainable.