Processing Site Research Articles

Effects of Human Nucleolus Upon Guest Viral-Life, Focusing in COVID-19 Infection: A Mini- Review.

The nucleolus is a subcellular membrane-less structure of eukaryotic cells. In 1965, in a world’s southern summer summit in Uruguay, the role of the nucleolus as the site of ribosome synthesis, biogenesis, and processing of tRNA was conclusively established. Today, accumulating evidence confirm the multiple functions of the nucleolus, including tRNA precursor processing, cell stress sensing, as well as being influential in gene silencing, senescence, lifespan, DNA damage response (DDR), and cell cycle regulation. Therefore, nucleolopathy is observed in various human diseases. Modern advances have provided fundamental insights concerning how and why the nucleolus is targeted by different pathogenic organisms. Viruses are major organisms that disrupt the normal function of the nucleus and produce nucleoli proteins for facilitating the replication of viruses causing viral infections. In this review, we focus on the possible role of nucleoli upon coronavirus infections, particularly in coronavirus disease 2019.

Photography after Discard Studies: the case of Agbogbloshie

Jacob Badcock’s article examines some shared problems in photography and pollution science by analysing two photographic series on Agbogbloshie, an electrical and electronic waste processing site in Ghana: The Hell of Copper (2008) by Nyaba Leon Ouedraogo and Permanent Error (2010) by Pieter Hugo. The article outlines how contemporary lens-based art concerned with representing environmental issues and scientific research attempting to measure pollution can be, despite benevolent intentions, guilty of reproducing colonial power relations.

Identification of Cognate Protease/Substrate Pairs by Use of Class-Specific Inhibitors.

Many proteins are regulated post-translationally by proteolytic processing. This includes plant signaling peptides that are proteolytically released from larger precursor proteins. The proteases involved in the biogenesis of signaling peptides and in regulation of other proteins by limited proteolysis are largely unknown. Here we describe how protease inhibitors that are specific for a certain class of proteases can be employed for the identification of proteases that are responsible for the processing of a given target protein. After having identified the protease family to which the processing enzyme belongs, candidate proteases and the GFP-tagged target protein are agro-infiltrated for transient expression in N. benthamiana leaves. Cleavage products are analyzed on immuno-blots and specificity of cleavage is confirmed by co-expression of class-specific inhibitors. For the identification of processing sites within the target protein, cleavage product(s) are purified by immunoprecipitation followed by polyacrylamide gel electrophoresis and analyzed by mass spectrometry.

Toward Autonomous Vehicles and Machinery in Mill Yards of the Forest Industry: Technologies and Proposals for Autonomous Vehicle Operations

The use of autonomous systems on the wood processing sites of forest industries can significantly increase safety, productivity, and efficiency by reducing the number of monotonous and dangerous tasks conducted by human labor utilizing heavy machinery. However, autonomous machines are challenging and difficult to implement in the mill yards environment because of the dynamic and complex working environment, and partly unstructured processes. The inherent complexity of wood handling and storage tasks is requiring significant human expertise. Fast advancements in sensor technologies, machine learning techniques, and increases in the computational power enable progress in automated operation framework and algorithms development. This opens the door to introduce novel autonomous systems into this environment. With the aim of gaining a better understanding of current issues and facilitating optimal strategies for deploying high-level autonomous systems for the mill yard environments, this study: (1) Utilized a systematic literature review to map current autonomous technologies and algorithms suitable for adoption by the forest industry in automation of vehicles working in mill yards; (2) Summarizes and discusses the potential feasibility of the considered sensors and systems, adaption strategy, and high-level autonomous machinery implementation challenges in the mill yard environment; and (3) Proposes a system framework that integrates multiple technologies to enable autonomous navigation and material handling in mill yards. The study is the first of its kind as a comprehensive study on autonomous vehicles and machinery in mill yard environments. Our novel framework aids in the identification of follow-up research areas and thus promotes the adoption and use of complex autonomous systems in industrial environments.

Peptide Backbone Modifications for the Assessment of Cleavage Site Relevance in Precursors of Signaling Peptides.

The physiological relevance of site-specific precursor processing for the biogenesis of peptide hormones and growth factors can be demonstrated in genetic complementation experiments, in which a gain of function is observed for the cleavable wild-type precursor, but not for a non-cleavable precursor mutant. Similarly, cleavable and non-cleavable synthetic peptides can be used in bioassays to test whether processing is required for bioactivity. In genetic complementation experiments, site-directed mutagenesis has to be used to mask a processing site against proteolysis. Peptide-based bioassays have the distinctive advantage that peptides can be protected against proteolytic cleavage by backbone modifications, i.e., without changing the amino acid sequence. Peptide backbone modifications have been employed to increase the metabolic stability of peptide drugs, and in basic research, to investigate whether processing at a certain site is required for precursor maturation and formation of the bioactive peptide. For this approach, it is important to show that modification of the peptide backbone has the desired effect and does indeed protect the respective peptide bond against proteolysis. This can be accomplished with the MALDI-TOF mass spectrometry-based assay we describe here.

FEASIBILITY ANALYSIS AND DESIGN PROJECTION OF WASTE MANAGEMENT SYSTEM IN BALIKPAPAN

Waste generation in Balikpapan from 2016 to 2020 continues to increase by 443 to 482 tons per day. One of the efforts of DLH Balikpapan is to optimize the MRF and ITF facilities, as well as to analyze how much these facilities are needed to reduce waste generation at final disposal sites (Landfill) Manggar and enhance the durability of the landfill. The approach utilized in this study employs direct observation methods, such as data collection results on the amount of waste input and output, and secondary data, such as geometric methods and all data on waste generation in Balikpapan. The calculation of the feasibility and effectiveness analysis of the MRF inorganic waste processing facility using the recovery factor approach obtained 60.3%, while the ITF organic waste processing got a result of 45.7%.With the current condition, Manggar Landfill can be used until the end of 2025, Manggar Landfill can be used until the end of 2025. By optimizing garbage processing in these two facilities, the MRF has expanded the service coverage to 3 urban villages and has a projected age of up to 2033. It will be optimized for ITF processing facilities by utilizing process biogas. The primary objective of this research is to determine how many additional processing sites are needed starting with household waste sources thus, the calculation findings show that an additional six units of MRF facilities and ten units of ITF facilities are required. As a result, with the addition of inorganic and organic waste processing sites, Manggar's Landfill estimated age is extended until 2028.

Scanning electron microscopy for differentiating charred endocarps of Rhus/Toxicodendron species and tracking the use of the lacquer tree and Asian poison ivy in Japanese prehistory

The use of natural lacquer for preservation and decoration of various commodities is a remarkable invention. In Japan lacquer production dates back to the Neolithic Jomon period and has been mainly based on the sap extracted from the lacquer tree (Toxicodendron vernicifluum (Stokes) F.A. Barkley). However, it is still unclear, if lacquer production evolved in Japan independently or was introduced from Neolithic China, another centre of early lacquer production. A debate also revolves around the origin of the lacquer tree itself and whether it occurs in Japan naturally or was introduced from the continent along with the skills required for lacquer production. Records of Rhus/Toxicodendron fruit remains, recovered from cultural layers of archaeological sites and natural palaeoenvironmental archives across Japan, provide an opportunity to answer the existing questions. This paper presents a method for differentiating charred endocarps of the six Rhus/Toxicodendron species growing in Japan, which is not feasible based on size or morphological properties of whole fruits or endocarps. To develop this method, we used a set of modern reference fruits. Identification is based on the species-specific tissue structure of the charred endocarp in longitudinal sectional view observed by scanning electron microscopy (SEM). We suggest a simple identification key that is based on two prominent traits of the endocarp’s tissue structure, i.e., tissue alignment and density. The method was successfully applied to an abundant record of charred Rhus/Toxicodendron endocarps recovered by flotation from Okhotsk culture layers dating to ca. 490–880 CE at the multi-component Hamanaka 2 archaeological site, Rebun Island, Hokkaido region, northern Japan. The recovered endocarps belong to Asian poison ivy (Toxicodendron orientale Greene, 1905) and the chronology of the archaeobotanical assemblages suggests that fruits and/or other parts of this plant were used by different local populations over a period of up to 3000 years (Final Jomon to Classic Ainu period). This indicates that not only lacquer tree was an important economic plant in Japanese prehistory, but also other Rhus/Toxicodendron species were used for unknown purpose. While use of T. orientale for medical effects or as an alternative source for lacquer production seems possible, we hypothesise that at Hamanaka 2 it was utilised for its high tannin content to tan hides and furs of sea mammals. This interpretation emphasises the discussed function of Hamanaka 2, famous for its rich zooarchaeological record of sea mammal remains, as an important site of marine hunting and raw material processing.

Image Preprocessing Method in Radiographic Inspection for Automatic Detection of Ship Welding Defects

Welding defects must be inspected to verify that the welds meet the requirements of ship welded joints, and in welding defect inspection, among nondestructive inspections, radiographic inspection is widely applied during the production process. To perform nondestructive inspection, the completed weldment must be transported to the nondestructive inspection station, which is expensive; consequently, automation of welding defect detection is required. Recently, at several processing sites of companies, continuous attempts are being made to combine deep learning to detect defects more accurately. Preprocessing for welding defects in radiographic inspection images should be prioritized to automatically detect welding defects using deep learning during radiographic nondestructive inspection. In this study, by analyzing the pixel values, we developed an image preprocessing method that can integrate the defect features. After maximizing the contrast between the defect and background in radiographic through CLAHE (contrast-limited adaptive histogram equalization), denoising (noise removal), thresholding (threshold processing), and concatenation were sequentially performed. The improvement in detection performance due to preprocessing was verified by comparing the results of the application of the algorithm on raw images, typical preprocessed images, and preprocessed images. The mAP for the training data and test data was 84.9% and 51.2% for the preprocessed image learning model, whereas 82.0% and 43.5% for the typical preprocessed image learning model and 78.0%, 40.8% for the raw image learning model. Object detection algorithm technology is developed every year, and the mAP is improving by approximately 3% to 10%. This study achieved a comparable performance improvement by only preprocessing with data.

Determining Suitable Locations for Regional Waste Final Processing Site (WFP) Using the Simple Additive Weighting (SAW) (Case Study: Malang City and Malang Regency)

Garbage or waste is basically a residual material resulting from human activities and natural processes that have no economic value anymore. The volume of waste in Malang City and Malang Regency every year always increases, so the existing waste final processing site will no longer be able to accommodate the pile of garbage. Therefore the Malang City Government plans to collaborate with the Malang Regency Government in making an integrated regional waste landfill and processing site. In this study, an analysis of the determination of the appropriate location for regional waste final processing for Malang City and Malang Regency was carried out using the Simple Additive Weighting (SAW) method. The SAW method is a weighted summation method, which can make a more precise assessment, based on the predetermined criteria and preference weights. Preference weights were determined by pairwise comparison method. The criteria used are geological hazardous areas, distance from drinking water sources, land slope level, distance from settlements, protected areas and distance from airports. The final result in this study is a map of the appropriate location for a regional waste final processing site and an analysis of the location for the best regional final processing site. The location map is classified into three, namely: not feasible with a total area of 54,774.33 ha, less feasible with a total area of 170,846.49 ha and feasible with a total area of 130,096.63 ha.

Reductions in ApoE and GPx4 highlight the Alzheimer's disease lipid raft vulnerability.

AD is associated with increases in lipid peroxidation and decreased antioxidant defense. Little is known about antioxidant defense of brain lipid rafts which are the site of APP processing. Oxidative damage to lipid rafts increases amyloid production. Lipid hydroperoxidases, glutathione peroxidase 4 (GPx4) and peroxiredoxin 6 (Prdx6) are potential detoxifying enzymes capable of reducing oxidized phospholipids and oxysterols within cellular membranes. We hypothesized that lipid hydroperoxidases would be reduced in ApoE4 carriers, which also have deficient cholesterol transport. To determine this, we investigated the status of the AD lipid raft by ApoE isoform in human frontal cortex. Lipid rafts were isolated (Invent Biotechnologies; LR-039) from postmortem AD (ApoE3,3, ApoE3,4, and ApoE4,4) and age-matched controls (ApoE3,3) and were analyzed by western blot and enzymatic assay. Lipid raft ApoE protein level of AD brains showed large decreases: 43% (Ctl vs AD3,3; p<0.01), 53% (Ctl vs AD3,4; p<0.001) and 70% (Ctl vs AD4,4; p<0.0001). Total cholesterol content of lipid rafts decreased 25% (Ctl vs AD; p<0.01). GPx4 protein of lipid rafts decreased 30% (Ctl vs AD3,3; p<0.01), 45% (Ctl vs AD3,4; p<0.0001) and 57% (Ctl vs AD4,4; p<0.001). AD brains from ApoE4,4 carriers had 36%(p<0.01) lower GPx4 localization than ApoE3,3. Prdx6 localization was not detected in the lipid rafts. Reductions in ApoE, total cholesterol, and GPx4 in the AD lipid raft suggests its vulnerability during AD. Furthermore, many of these decreases were ApoE isoform dependent, suggesting that antioxidant defense may differ by ApoE isoform.

Pelatihan dan Pendampingan Pengelolaan Sampah Organik Menjadi Kompos Dengan Metode Takakura di Desa Waduwani

Garbage have been become big problem for our environment, especially the garbage at Waste Processing Site (TPA) on Waduwani Village. Several efforts have been made by government to reduce garbage volume. But these efforts have not been fully realized in the community. So that it is urgently needed an appropriate and comprehensive solution to reduce the volume of the garbage and its negative effect on environment. Training and assistance to make organic garbage to be the compost by using “Takakura method” is the one solution to reduce garbage problems. This activity is a Community Service for the community, especially women in Waduwani village, Woha sub-district of Bima, NTB. The objectives of this community service activity includes (1) Counseling the community to improve community knowledge and skills to make organic garbage properly and sustainably. (2) The management and training can reduce the volume of organic garbage generated from the house. (3) The results of training of compost made can be utilized by the community, especially to fertilize their plants. The method of implementing this activity is carried out in four stages, namely; socialization, Implementation program, Garbage processing, and finally Program evaluation. The results of Takakura Method are (1) IncreaseMothers knowledge and skills to manage the garbage (2) Reduce the garbage volume which generated at household/kitchen. (3) The results of make Takakura compost can be used directly by mothers to fertilize their plants.

UPAYA MENGOLAH LIMBAH SAMPAH MENJADI PELUANG SOCIAL ENTERPRISE EKONOMI KREATIF BAGI GENERASI-Z

The problem of waste in Indonesia has reached 175,000 tons/day which is equivalent to 64 million tons/year, which until now has been managed by being transported and dumped in the final processing site (TPA) because people still have the perception that waste is a waste material that is considered useless. The COVID-19 pandemic in 2020 has limited access to activities outside the home, ultimately changing consumer behavior by shifting the way to shop for their needs to online shopping. The study method utilized social media platform to distribute the online webinar information to gathered large number of participants. Thus, the study find that the Generation Z is a generation that is synonymous with digital technology and information, as a Collaborative Generation, a generation that knows the "sharing economy" is the foundation of hope to take advantage of waste problems as challenges and opportunities that can create a generation of Social Entrepreneurs who create waste into products that have bargaining power and competitiveness in the creative economy market through social media or e-commerce platforms.

Social support role of occupational safety and health implementation in informal sector during COVID-19 pandemic

This study was conducted in a limestone processing site in Gunungkidul Regency, Yogyakarta, with One group pretest-posttest design. The study was initiated by OSH training for social supports done by competent health cadres. There were 8 social supports from fellow workers (peer support) and 29 social supports from family members (family support). These social supports assisted workers for 4 weeks. Data was collected by using three scales i.e. Scale of OSH knowledge (8 items, Coefficient Alpha Cronbach Reliability 0.800), attitude towards OSH scale (10 items, Reliability 0.917) and Practice of OSH Scale (10 items, Reliability 0.804). Data was collected before and after role assistance by social supports. Data analysis was done by the Wilcoxon sign rank test (alpha: 0.05). The results showed that there was an increase in the average knowledge of OSH from 5.83 to 6.93 (p&lt;0.05), attitudes towards OSH from 26.23 to 30.76 (p&lt;0.05), and OSH implementation from 25.00 to 28.83 (p&lt; 0.05). It was proven that the involvement of social supports from peer as well as family could increase knowledge, attitude and OSH practice of limestone processing workers. To increase the accuracy of the study result, it is advisable that the further study use a control group.

Stephan Oroszlan and the Proteolytic Processing of Retroviral Proteins: Following A Pro

Steve Oroszlan determined the sequences at the ends of virion proteins for a number of different retroviruses. This work led to the insight that the amino-terminal amino acid of the mature viral CA protein is always proline. In this remembrance, we review Steve’s work that led to this insight and show how that insight was a necessary precursor to the work we have done in the subsequent years exploring the cleavage rate determinants of viral protease processing sites and the multiple roles the amino-terminal proline of CA plays after protease cleavage liberates it from its position in a protease processing site.

Morphological and chemical characterization of uranium and cerium nuclear forensics samples

• Uranium and cerium containing samples were examined as part of an international nuclear forensics exercise. • The main technique for characterization was the combined analysis by SEM-EDS/Raman of the surface. • Connection between the ingots was determined by uranium and cerium isotopic ratios as well as yttria and graphite surface contaminations. • Sample preparation history was interpreted based on surface contaminations, showing multiple impurities present in suggested production and processing sites. The objective of this paper is to describe nuclear forensics material characterization, as conducted in the Israeli National Nuclear Forensic Laboratory (INNFL) during an international exercise Collaborative Material Exercise 6 (CMX6) organized by the Nuclear Forensics international technical working group (ITWG). The characterized materials are uranium and cerium metal ingots. The goal was characterization of physical parameters, elemental and isotopic composition, morphology and chemical composition of the surface. Electron microscopy and Raman spectroscopy techniques were combined for morphological, structural and elemental study of the surface. The information from these analyses was used to answer the exercise questions, comparing the materials (labeled ES-1 and ES-2) to each other and to other samples described in the scenario. The INNFL investigation identified a connection between ES-1 and ES-2 based on several indicators, including the isotopic composition of the uranium present in both samples, as well as other surface contaminations, mainly yttria and graphite. Based on these finding the INNFL established a connection between ES-1 and ES-2 to ES-3B (theoretical sample with uranium traces) and has not established a connection to ES-3A (theoretical sample of plutonium powder). ES1 and ES-2 have been suggestively connected to their theoretical source, by their isotopic ratio. The INNFL conclusion was that the theoretical source materials (oxide and fluoride) were converted into the actual materials.

Neural Correlates of Anti-appetite Medications: An fMRI Meta-analysis.

Food craving is a health issue for a considerable proportion of the general population. Medications have been introduced to alleviate the craving or reduce the appetite via a neuropharmacological approach. However, the underlying cerebral processing of the medications was largely unknown. This study aimed to meta-analyze existing neuroimaging findings. PubMed, Web of Science, and Scopus were searched to identify relevant publications. Original studies that reported brain imaging findings using functional magnetic resonance imaging (fMRI) were initially included. The reported coordinates of brain activation available from the studies were extracted and meta-analyzed with the activation likelihood estimation (ALE) approach via the software GingerALE. The overall analysis pooling data from 24 studies showed that the right claustrum and insula were the targeted sites of altered cerebral processing of food cues by the medications. Subgroup analysis pooling data from 11 studies showed that these sites had reduced activity levels under medications compared to placebo. The location of this significant cluster partially overlapped with that attributable to affective value processing of food cues in a prior meta-analysis. No brain regions were found to have increased activity levels by medications. These neural correlates may help explain the physiological effect of food consumption by anti-appetite and anti-obesity medications.

Precisely Monomeric Linear RNAs of Viroids Belonging to Pospiviroid and Hostuviroid Genera Are Infectious Regardless of Transcription Initiation Site and 5'-Terminal Structure.

Infectious dimeric RNA transcripts are a powerful tool for reverse genetic analyses in viroid studies. However, the construction of dimeric cDNA clones is laborious and time consuming, especially in mutational analyses by in vitro mutagenesis. In this study, we developed a system to synthesize a precisely monomeric linear RNA that could be transcribed in vitro directly from the cDNA clones of four viroid species. The cDNA clones were constructed such that RNA transcription was initiated at the guanine nucleotide of a predicted processing and ligation site in the viroid replication process. Although the transcribed RNAs were considered to possess 5′-triphosphate and 3′-hydroxyl termini, the RNA transcripts were infectious even without in vitro modifications. Additionally, infectivity was detected in the monomeric RNA transcripts, in which transcription was initiated at guanine nucleotides distinct from the predicted processing/ligation site. Moreover, monomeric viroid RNAs bearing 5′-monophosphate, 5′-hydroxyl, or 5′-capped termini were found to be infectious. Northern blot analysis of the pooled total RNA of the plants inoculated with the 5′-terminal modified RNA of potato spindle tuber viroid (PSTVd) indicated that maximum PSTVd accumulation occurred in plants with 5′-monophosphate RNA inoculation, followed by the plants with 5′-triphosphate RNA inoculation. Our system for synthesizing an infectious monomeric linear viroid RNA from a cDNA clone will facilitate mutational analyses by in vitro mutagenesis in viroid research.

Strategy for institutional development of the regional technical implementing unit (UPTD) of regional waste landfill of NTB Province

The Kebon Kongok Regional Landfill (TPA) is one of the final processing sites serving West Lombok Regency and Mataram City. The Landfill is managed by the Regional Technical Implementing Unit (UPTD) of the Regional Waste Landfill of NTB Province under the Department of Environment and Forestry of NTB Province. Currently, waste services in Mataram City and West Lombok Regency have not met the sanitation target. One of the factors that affects it is the financial problem where there is no budget flexibility which often causes difficulties in handling operations, especially at the beginning and end of the year. Therefore, the purpose of this study is to determine the institutional development strategy as an effort to increase the role of UPTD in solid waste services. The analytical method used is Force Field Analysis (FFA). Based on the FFA, it is found that the Total Weight Value (TNB) driving forces are more significant than the restraining forces; this means that UPTD of Regional Waste Landfill of NTB Province is encouraged to carry out institutional development. Therefore, the institutional development strategy is that UPTD applies for the Financial Management Pattern of Public Service Agencies (PPK-BLUD).

Analysis of waste processing potential in Gunung Putri District, Bogor Regency

The increase in waste generation in Gunung Putri Subdistrict, Bogor Regency, causes an increased burden at the Landfill Site (TPA). One of the efforts to reduce the burden of Landfill Site (TPA) is to maximize the Waste Processing Site (TPS) by applying the circular economy concept. This study aims to analyze the generation, composition, and potential of waste processing to have economic value. The sampling method used is a combination of purposive sampling and stratified random sampling. The results showed that with a population of 304,672 people and a waste generation rate of 0.48 kg/person/day, it is estimated that the waste generation in Gunung Putri District is 147,156.58 kg/day. The waste material in Gunung Putri Subdistrict consists of 52.86% organic waste components and 47.14% non-organic waste. Organic waste is dominated by food waste by 50.38%. From the results of the characteristic test of the waste, it is known that the water content is 63.16%, the ash content is 29%, the C/N ratio is 7.03%, and the calorific value of the waste is 1.924 kcal/kg by considering the amount of generation, composition, and characteristics of waste, which are then used with standards or criteria for waste processing technology from various references. Organic waste in Gunung Putri Subdistrict has good potential to be processed using the Black Soldier Fly (BSF) and organic waste processing with Peuyeumisasi Method (Biodrying) and recycling for non-organic waste.

The policy of the Surakarta City Parks and Hygiene Service in environmental management

Based on Law Number 18 of 2008 concerning Waste Management, Tempat Pembuangan Sampah Akhir (TPA) is a Final Processing Site. The goal is to process and return waste to environmental media in a way that is safe for humans and the environment. The problems seen in the Putri Cempo TPA are the worrying land conditions, environmental pollution conditions, and poor management that the Government must pay attention to, because these have an impact on the surrounding community. This also caused a lot of complaints from the local community. The purpose of this study was to analyze the role of the Department of Parks and Hygiene in Waste Management in Surakarta City. In this study, researchers used descriptive qualitative research methods with 15 informants. The results showed that the management of the Putri Cempo TPA was not adequate, as seen from the many obstacles that occurred in Putri Cempo, such as several damaged vehicles and waste transportation equipment, the waste management operational system that determines the volume of waste disposed of in the TPA, and waste operational activities depending on the environment. operational pattern, namely the method of sweeping, collection, transportation, and final disposal.