This article evaluates the efficiency, benefits and limitations of pre-concentration by density separation for process mineralogy studies of low-grade gold ore. The pre-concentration by density aims to generate a product with a high content of gold and to maximize the number of gold-bearing particles characterized, thereby increasing the representativity and reducing the number of polished sections to be analyzed. Pre-concentration by density was carried out for a low-grade sulfide gold ore (0.96 ppm) from the north of Brazil. The sample was ground to below 1.7 mm, sieved down to 0.037 mm and subjected to density separation, amalgamation of the heavy product and cyanide leaching of all products. Gold was assessed in each product to evaluate the separation recovery and distribution. The finer the particle size, the higher the gold distribution in the heavy product, due to a higher degree of liberation of gold and sulfides; these values varied from 65% to 84% in fractions below 0.60 mm and decreased from 40% to 13% in the coarser fractions. Regarding gold distribution, fractions finer than 0.84 mm indicates a notable increase on gold recovery by amalgamation, indication higher surface exposure.