Sample Pretreatment Procedure Research Articles

Residual behavior of the potential grain fumigant 1-octen-3-ol in wheat during fumigation and ventilation processes.

Our previous works indicated that 1-octen-3-ol has promising potential as an alternative grain fumigant. However, aside from its insecticidal efficacy, the presence of 1-octen-3-ol residues in grains must be investigated to assess its food safety profile. A convenient and sensitive QuEChERS based GC-MS/MS method was developed to detect residues of 1-octen-3-ol in wheat. The sample pretreatment procedures were optimized. The developed method showed good linearity (R2 =0.9999) and negligible matrix effects. The limit of detection (LOD) and limit of quantification (LOQ) for 1-octen-3-ol were 0.003 and 0.01 mg kg-1 , respectively. Recoveries at spiked concentration levels of 0.01, 0.5, 10, 100 and 200 mg kg-1 ranged from 90.8% to 112.4%, with relative standard deviations (RSDs, n=5) ranged from 1.2 to 7.5%. In the fumigation process during wheat storage, there were positive correlations between the accumulation rate and fumigation concentration as well as between the accumulation amounts and fumigation time. In the ventilation process, temperature significantly affected the dissipation dynamics of 1-octen-3-ol in wheat, and the t1/2 values at ventilation temperatures of 30 and 5°C for 0.1μL mL-1 were 0.16 and 21.80 days, respectively, representing a 136-fold difference. Preservers can regulate the ventilation temperature to achieve different goals, with either a long duration period for long-term storage or rapid dissipation for quick food consumption. This study provides guidance on the reasonable usage of 1-octen-3-ol on wheat and other stored grains. © 2021 Society of Chemical Industry.

Determination of Abiraterone and Its Metabolites in Human Serum by LC-ESI-TOF/MS Using Solid-phase Extraction.

We developed and validated a liquid chromatography-electrospray ionization-time of flight/mass spectrometry method for the determination of abiraterone (Abi) and its metabolites (Δ4-Abi, 3-keto-5α-Abi, 3α-OH-5α-Abi and 3β-OH-5α-Abi) in human serum using Abi-d4 as the internal standard. As a pretreatment procedure of serum samples, solid-phase extraction based on a silica-gel cartridge was used. The relative recovery of Abi and its metabolites was over the ranges of 84.5 - 109.2% at a concentration of 6.0 ng mL-1 for Abi and 0.6 ng mL-1 for its metabolites. The method was free from matrix effects. The calibration curve of Abi was linear over the range of 2.0 - 400 ng mL-1 and those of its metabolites over the ranges 0.2 - 40 ng mL-1. The results of the intra- and inter-day accuracy and precision data were within the FDA acceptance criteria. The optimized method was applied for the determination of Abi and its metabolites in human serum after oral administration of Abi acetate.

Determination of K869, a Novel Oxime Reactivator of Acetylcholinesterase, in Rat Body Fluids and Tissues by Liquid-Chromatography Methods: Pharmacokinetic Study

Oxime reactivators of acetylcholinesterase (AChE) represent an integral part of standard antidote treatment of organophosphate poisoning. Oxime K869 is a novel bisquaternary non-symmetric pyridinium aldoxime with two pyridinium rings connected by a tetramethylene bridge where two chlorines modify the pyridinium ring bearing the oxime moiety. Based on invitro assays, K869 is a potent AChE and butyrylcholinesterase (BChE) reactivator. For the investigation of the basic pharmacokinetic properties of K869 after its intramuscular application, new HPLC-UV and LC-MS/MS methods were developed and validated for its determination in rat body fluids and tissues. In this study, the SPE procedure for sample pretreatment was optimized as an alternative to routine protein precipitation widely used in oxime pharmacokinetics studies. K869 oxime is quickly absorbed into the central compartment reaching its maximum in plasma (39±4μg/mL) between 15 and 20min. The majority of K869 was eliminated by kidneys via urine when compared with biliary excretion. However, only a limited amount of K869 (65±4ng/g of brain tissue) was found in the brain 30min after oxime administration. Regarding the brain/plasma ratio calculated (less than 1%), the penetration of K869 into the brain did not exceed conventionally used oximes.

A New Enrichment Method for Quantification of 5-Hydroxymethylfurfural by Indirect Flame Atomic Absorption Spectrometry in Honey and Jam Samples.

Because of increasing amounts of 5-hydroxymethylfurfural (5-HMF) in processed foods and the complexity of the matrix, monitoring of trace 5-HMF requires accurate and reliable methods. Hence, an efficient sample pretreatment procedure is necessary for extraction and preconcentration of 5-HMF from the matrix. In this study, a new and efficient sample preparation method utilizing ultrasound-assisted-cloud point extraction (UA-CPE), indirectly followed by flame atomic absorption spectrometry (FAAS), was introduced for the monitoring of trace amounts of 5-HMF in honey and jam samples. With this method, hydroxylamine was used as a derivatizing agent in the presence of Mn(II) and an anionic surfactant, SDS for extraction of 5-HMF at pH 9.0. For dispersing reagents in sample solution, low amounts of mixed surfactant, triton X-45 and SDS were mixed and fast-injected into the extraction media. A cloudy solution formed, and after reaction of 5-HMF with reagents, the cloudy solution was centrifuged. The extracted 5-HMF in the surfactant-rich phase was dissolved in acidic acetone and indirectly analyzed by FAAS. The method showed a detection limit of 1.27 μg/L in linear working range of 4-240 μg/L, good precision (2.3-6.5%), and recovery rates (93.5-97%) after preconcentration of 70-fold. Within this study, an accurate and reliable method for the indirect quantification of 5-HMF in selected samples was successfully developed with a sensitivity improvement factor of 30.6. The figures of merit for the developed indirect method were appropriate. The applicability of the method for the analysis of 5-HMF in processed foods was excellent.

Determination of propofol in human plasma with C18 pipette-tip based solid-phase extraction followed by liquid chromatography atmospheric-pressure chemical ionization tandem mass spectrometry analysis

Propofol is a widely used intravenous anesthetic for sedation during the surgery and worldwide propofol abuse has been frequently reported also. As an essential procedure for sample pretreatment, solid-phase extraction (SPE) is generally time-consuming and labor-intensive, which hindered the further improvement in the analysis of propofol from biological fluids. In this study, a rapid C18 pipette-tip based SPE method was developed to pretreat human plasma samples directly, bypassing the need for protein precipitation. The experiment conditions were optimized for the best extraction recovery (23.6 ± 4.1 %). After pretreatment, the plasma propofol was determined with liquid chromatography atmospheric-pressure chemical ionization-tandem mass spectrometry (LC-APCI-MS/MS) using propofol-d17 as the internal standard. The quantification method showed good linearity in the range of 0.005–5 μg mL−1 (r2 = 0.9992). The sensitivity, specificity, accuracy (90.0–113.3 %), precision (2.0–8.9 %), and stability (95.6–102.1 %) were satisfied for bioanalytical analysis. Finally, the concentrations of propofol in the plasma of a patient under anesthesia were determined with the proposed method and compared with the theoretical concentrations calculated with a population pharmacokinetics (popPK) model. In general, this work provided a rapid and straightforward method for the study of propofol in pharmacokinetics and forensic science.

Development and validation of GC/MS method for simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) in pork meat matrix

AbstractThe major processes for introducing polycyclic aromatic hydrocarbons (PAHs) in food are smoking and grilling of different products. But in addition, PAHs can permeate in the food chain due to their high lipophilicity and ability to be accumulated in specific tissue, through contaminated animal feed. Further, when some parts of these animals are marketed as food, the accumulated PAHs can go to the human organism. Some of them are classified as highly toxic, carcinogenic and mutagenic for animal and human organisms so they are under consideration of International and European legislation. This work reports development and validation of simple and fast GC/MS method for 16 PAHs determination. Comparison of two methods for sample preparation in pork meat matrix standard extraction/saponification procedure and modified QuEChERS method is also done. In addition, this paper report the calibration step of instrument and a recovery study for 16 PAHs in model pork meat, using modified QuEChERS procedure for sample pretreatment. The calibration step with accessible and suitable for use in real laboratory conditions internal standard (chrysene D12) is done in the range 10–100 ppb using toluene as solvent. The obtained results show very good linearity (R2 = 0.99 to 1.00). For the recovery study six model samples were spiked with 16 PAHs and they all are subjected to QuEChERS procedure. The recovery is calculated and the obtained data (71–120%) is in a good correlation with requirements of international legislation. Finally, LOD values for all 16 investigated compounds of modified GC/MS method and for the instrument were determined.

MIL-101 (Cr) @ graphene oxide-reinforced hollow fiber solid-phase microextraction coupled with high-performance liquid chromatography to determine diazinon and chlorpyrifos in tomato, cucumber and agricultural water

An effective, sensitive, relatively-fast and cost-effective method was developed to determine two types of selected organophosphorus pesticides (OPPs) including diazinon and chlorpyrifos in tomato, cucumber and agricultural water samples through applying MIL-101@ graphene oxide-reinforced hollow fiber solid-phase microextraction (MIL-101@GO–HF–SPME). The extracted analytes were desorbed via organic solvent and analyzed through high-performance liquid chromatography-ultraviolet detection (HPLC-UV) after extraction. MIL-101@ GO sorbent which was previously prepared and characterized was first dispersed in methanol by ultrasonication and then immobilized into the pores of a hollow fiber (HF). Several factors involved in MIL-101@GO–HF–SPME experiment such as desorption volume, ionic strength, desorption time, sample pH, extraction time and stirring rate were screened via Plackett–Burman design and then optimized through Box–Behnken design with the purpose of reaching the highest extraction efficiency. The above method showed a good linear range (1–500 μg L−1) with coefficient of determination higher than 0.9948, low limits of determination (LODs, 0.21 and 0.27 μg L−1), acceptable limits of quantifications (LOQs, 0.72 and 0.91 μg L−1), good enrichment factors (EFs, 49 and 41), acceptable absolute recoveries (AR%, 49 and 41%) and good spiking recoveries (88–104%) under the optimized condition at three different spiked levels for chlorpyrifos and diazinon, respectively. It is worth mentioning that due to the clean-up function of HF, there is no time-consuming sample pretreatment procedure (e.g. filtration or centrifugation) prior to the microextraction. Therefore, the presented method took advantage of both excellent adsorption performance of MIL-101@GO and the clean-up function of HF. The results confirmed that the presented method would be promising for the analysis of various types of these pesticides in environmental and vegetable samples.

Simple and rapid analysis of phthalate esters in marine sediment using ultrasound-assisted extraction combined with gas purge microsyringe extraction followed by GC–MS

Phthalate esters (PAEs) are a class of the emerging pollutants that pose a potential environmental threat to marine ecosystems. In this study, a simple analytical method using ultrasound-assisted extraction combined with gas purges microsyringe extraction (GP–MSE) coupled with GC–MS was utilized for the reliable and rapid determination of PAEs in different types of marine sediment. The analytical results showed that the method exhibited excellent reproducibility, linear responses, and detection limits, which verified the suitability of the method for the determination of PAEs in marine sediment. This approach requires minimal reagents, solvents, and sample pretreatment procedures as well as a short analysis time; thus, procedural blanks can be kept to a minimum. This method was demonstrated to be a highly efficient and sensitive quantitative analytical method for the simple detection of PAEs in marine sediment.

A Rapid and Simple Gas Chromatographic Method for Direct Determination of Safrole in Soft Drinks

A simple and rapid method was developed to determine the safrole and isosafrole in soft drink using a megapore semi-polar column (CP-SIL 8CB, 30 m × 0.53 mm, 1.5 μm) with direct injection gas chromatography. Direct quantitative analysis of safrole and isosafrole in soft drinks was carried out without any sample pretreatment procedure. The water soluble compound 1,4-dihydroxybenzene (DHB) was used as the internal standard. The detection limit for safrole and isosafrole was 0.25 μ/mL. A recovery study was performed using one of the soft drinks by spiking 1 mL with safrole and isosafrole at 5.0 and 10.0 μg, respectively. The recovery was found in the range of 98-108% with coefficients of variation being less than 8.7%. Twenty-five commercial soft drinks were analyzed by the current method, and results indicated that 20 out of 25 soft drink samples contained safrole andlor isosafrole, and the amount of safrole was 3-5 fold over the regulated amount, 1 μ/mL.

Determination of glucosamine content in nutraceuticals by capillary electrophoresis using in-capillary OPA labeling techniques

Capillary electrophoresis (CE) procedure was developed for determining glucosamine content in nutraceuticals. Glucosamine was labeled with o-phthalaldehyde (OPA) by mixing with the OPA reagent (4.5 mM OPA, 4.5 mM 2-mercaptoethanol dissolved in 20 mM borate buffer, pH 9.3). The fluorescent OPA-glucosamine adduct was separated and detected at A340 within 3 min. The pre-capillary labeling reaction can be performed by sequentially injecting OPA/sample/OPA segments into the separation capillary; the in-capillary labeling technique simplified the sample pretreatment and improved its reproducibility. The linear dynamic range of the in-capillary labeling CE method was 0.1-30 mM. No sample pretreatment procedure was required for tablets containing mainly starch or other sugar as the vehicles.

A gas chromatographic method for determination of nicotinamide, paraben esters and caffeine in commercial health drinks, tonic drinks and cold formulas

A simple and rapid method was developed to simultaneously determine the nicotinamide, paraben as well as caffeine by using megapore immediate-polar column (CP-Sil 24CB, 30 m x 0.53 mm, 1.5 μm) with direct injection gas chromatography. Direct quantitative analysis of nicotinamide, paraben esters and caffeine in health drinks, tonic drinks, and cold formulas was carried out without any sample pretreatment procedure. The water soluble compound 1,9-nonanediol was used as an internal standard. The detection limit for nicotinamide, paraben esters and caffeine was 2 μg/mL. Recovery studies were performed using 1 mL of vitamin B drink, tonic drink and cold solution, each spiked with nicotinamide, paraben esters and caffeine at 100.0 μg, respectively. The recoveries for nicotinamide were 96, 102 and 105%, respectively, and the coefficient of variation was less than 7.2%. The recoveries for butylparaben were 105, 95, and 107%, respectively, and the coefficient of variation was less than 8.3%. The recoveries for caffeine were 101, 95 and 104%, respectively, and the coefficient of variation was less than 7.5%. Fifty-two commercial health drinks, tonic drinks and cold solutions were analyzed by the current method. The nicotinamide content was found as: 20.3-241.7, 0-210.7 and 0-246.8 μg/mL, respectively; the isobutylparaben content was found as: 0-54.1, 0-42.9 and 0-134.1 mg/mL; the butylparaben content was found as: 0-106.8, 0-120.1 and 0-184.6 μg/mL; and the caffeine content was found as: 0-433.2, 0-80.7 and 472.3-1742.9 μg/mL. These results indicated that 6 out of 18 commercial health drinks, 2 out of 12 commercial tonic drinks, and 2 out of 22 commercial cold formulas exceeded the Taiwan nicotinamide recommended daily nutrient allowances (RDNA, 14.4 μg) level. Furthermore, 25 out of 52 samples labeled 'preservative-free' were detected to be preservative-containing products. The total preservative contents in some test samples exceeded the regulation levels of 100 μg/mL; and 27 out of 30 samples were detected to be caffeine-containing products. These results were inconsistent with the CNS (Chinese National Standard) rules.

A Rapid Method for Direct Determination of Levulinic Acid in Soy Sauce

A simple and rapid method was developed to determine the levulinic acid level in soy sauce using megapore polar column (CP-Wax, 30 m × 0.53 mm) with splitless direct injection gas chromatography. Direct quantitative analysis of levulinic acid in soy sauce was carried out without any sample pretreatment procedure. A water-soluble compound, 1,6-hexanediol, was used as the internal standard. The detection limit for levulinic acid was 10 μg/mL. Recoveries from soy sauce and pickled condiment liquid were performed by spiking 2.5, 5.0, or 10.0 mg to 1 mL of test samples and were found to be at the range of 98∼103% with coefficients of variation less than 6.3%. Fourty-six food samples including animal and vegetable protein hydrolysate, pickle condiment liquid, and soy sauce were analyzed using the proposed method. Levulinic acid contents in pickled condiment liquid were found to be 2.1∼4.7 mg/mL and in soy sauce were 7.8∼24.5 mg/mL, which were higher than CNS regulated level (1.0 mg/mL), indicating commercial pickles and soy sauce might be adulterated with vegetable protein hydrolysate. These results were inconsistent with the labeling of "100% fermented soy sauce" on the packages.

A rapid and simple gas chromatographic method for direct determination of nicotinamide in commercial vitamins and tonic drinks

A simple and rapid method was developed to determine the presence of nicotinamide in tonic drinks using the megapore semi-polar column (CP-Sil 8 CB, 30 m x 0.53 mm) with direct injection gas chromatography. Direct quantitative analysis of nicotinamide in amino acid drinks, vitamin drinks, essence of chicken and tonic drinks was carried out without any sample pretreatment procedure. Water soluble compound 1,9-nonanediol was used as an internal standard. The detection limit for nicotinamide was 2-5 μg/mL. Recovery studies were performed using 1 mL of vitamin drink and tonic drink, each spiked with nicotinamide at 105.5, and 211.0 μg, respectively. The recoveries were found in the range of 94-99% and 93-108%, respectively. The coefficients of variation being less than 9.8%. Forty-two commercial amino acid drinks, vitamin drinks, essences of chicken and tonic drinks were analyzed with this method. The nicotinamide contents were found as: 131-246, 24-112, 0-134 and 0-263 μg/mL, respectively. These results indicated that, one bottle each of the amino acid drink, vitamin drink, essences of chicken and tonic drink contain nicotinamide levels of: 39-74, 5-22, 0-10 and 0-21 mg/bottle. The results also showed that in 12 out of 4 of commercial amino acid and vitamin drinks, the nicotinamide exceeded the ROC's acceptable daily intake (RNDA, 14.4 mg) level; and 3 out of 10 commercial tonic drinks exceeded the ROC's RNDA level for nicotinamide.

Recent applications of covalent organic frameworks and their multifunctional composites for food contaminant analysis.

Food safety issues are a great concern of the international community. Many methods to analyze and detect food contaminants have been widely researched because both biological and chemical contaminants in food can cause severe damage to human health. Several adsorbent materials have been utilized in the complicated procedure of food sample pretreatment. Covalent organic frameworks (COFs) present enormous application potential due to their tailorable skeletons and pores and fascinating features. In this review, recent applications of COF-based adsorbents for the extraction and analysis of food contaminants, including polycyclic aromatic hydrocarbons, biogenic amines, pesticides, endocrine-disrupting chemicals, heavy metal ions, illegal additives, and biotoxins, from complex samples are summarized. Current challenges and future prospects are also discussed.

Quantification of fat-soluble vitamins and their metabolites in biological matrices: an updated review.

Fat-soluble vitamins (FSVs) are micronutrients essential in maintaining normal physiological function, metabolism and human growth. Ongoing increased awareness regarding FSV concentrations and their impact on human growth along with disease progression warrant the need of developing selective and sensitive analytical methods.LC-MS/MS is currently the method of choice for accurate quantitation of FSVs. However, there are multiple approaches for extraction, separation and calibration of FSVs in biological matrices. This review discusses recent LC-MS/MS methods for the simultaneous quantification of FSVs in biological matrices and summarizes sample pretreatment procedures, chromatographic conditions and calibration approaches. Current challenges and clinical applications in various disease states are also highlighted.

Direct-immersion SPME in soy milk for pesticide analysis at trace levels by means of a matrix-compatible coating

This study demonstrates a newly developed PDMS/DVB/PDMS fiber's suitability for the determination of pesticides in soy milk via direct-immersion solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry, eliminating the need for extensive sample pre-treatment procedures. Fouling accumulation on the coating surface was further minimized by implementing rapid and effective pre- and post-desorption cleaning steps. Under optimum conditions, the fiber was used to perform over 120 extractions while maintaining RSD values of less than 24.5% for 10 extracted pesticides. By comparison, the RSD values ranged from 8.4% to 42.8% over 80 extractions using a commercial PDMS/DVB fiber. The optimized conditions were used to fully validate a quantitative method for the targeted analytes by matrix-matched calibration and isotopically labeled internal standard correction. Significantly, the proposed method was able to achieve limits of quantitation (1–2.5 μg/kg) for the targeted analytes that were below the Maximum Residue Levels mandated for soy-based products. Accuracy, intra- and inter-day repeatability were also satisfactory. The proposed PDMS/DVB/PDMS fiber dramatically improved repeatability and suitability for direct-immersion SPME in soy milk, and represents a good alternative to other extraction methods for high-throughput quantitative analysis of pesticide residues in soy-based products.

Evaluation of different food matrices via a dihydropteroate synthase-based biosensor for the screening of sulfonamide residues

ABSTRACT In a previous study, we developed a dihydropteroate synthase (DHPS)-based biosensor for multi-sulfonamides (SAs), which could detect 29 SAs with IC50 values below 100 ng mL−1. The purpose of the present study was to apply the DHPS-based biosensor to different food matrices and evaluate the influence of matrix effects on the performance of the biosensor by using sulfamethazine (SMZ) as a reference analyte. The results show that different sample pretreatment procedures were required for each food sample to ensure the accuracy and precision of the biosensor. The limit of detection (LOD) for SMZ in chicken, pork, egg and honey was 17.82, 20.55, 23.22, and 5.57 μg kg−1, respectively. The recovery values ranged from 47.6% to 135.1%, and the coefficients of variation (CV) were less than 25.6%. Taken together, our findings show that the DHPS-DHPPP biosensor is a suitable screening method for the screening of multi-SAs in different food matrices.

Methylmercury Determination in Fish by Direct Mercury Analyzer

There are two methods for quantifying methylmercury (MeHg) in fish using GC-electron capture detection (ECD): AOAC INTERNATIONAL Official Method 988.11 and the Korean Food Code (KFC) method. Both of these methods consume a large amount of chemicals and require long pretreatment times because of several complicated MeHg extraction steps. In this study, a new method for the simple and rapid determination of MeHg in fish has been developed. The method is based on the investigation of oxygen combustion-gold amalgamation using a direct mercury analyzer (DMA) after the complete removal of MeHg by organic extraction and back-extraction to an aqueous medium. The DMA is suitable for the analysis of both solid and liquid materials and has a good detection limit. The developed method was validated by comparing the MeHg recoveries (%) of both certified reference materials and the market-purchased fish samples with the MeHg concentration obtained using the KFC method. The following parameters pertaining to the developed method were established: detection limit, 1.02 μg/kg; LOQ, 3.09 μg/kg; linearity (r), 0.9998; range, 0.1-300 μg/kg; and recovery, 95-97%. Our method is a promising alternative by virtue of its much simpler and faster sample pretreatment procedure, with a MeHg recovery as high as that of the KFC method. The developed method enables the simultaneous analysis of total Hg and MeHg with only DMA equipment.

A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection.

Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membranes combined with hydrophilic narrow capillaries are introduced into devices, in order to reduce the residual volumes of blood plasma. An interference fit is used to ensure no leakage of blood or cells. There is desired trapping efficiency of blood cells in the devices. The method provides high efficiency with a plasma extraction yield of 71.7% within 6 min, using 60 μL of undiluted whole human blood with 45% haematocrit. The influence from structural parameters on the separation kinetics and the dependence of the haematocrit levels on the separation efficiency are also investigated. The total protein detection shows considerable protein recovery of 82.3% in the extracted plasma. Thus, the plasma separation unit with a very simple structure is suitable for integrating into microfluidic devices, presenting promising prospects for clinical diagnostics as well as for point-of-care testing applications.

Development of a New and Fast Extraction Method Based on Solvent-Assisted Dispersive Solid-Phase Extraction for Preconcentration and Trace Detection of Atrazine in Real Matrices.

Because of trace amounts of atrazine in water samples and the complexity of the matrix, direct trace monitoring of atrazine is not feasible by the abovementioned techniques. Hence, an efficient sample pretreatment procedure is necessary for cleanup and preconcentration of atrazine from sample matrices. In the current paper, a new and efficient sample preparation method named solvent-assisted dispersive solid-phase extraction (SA-DSPE), followed by HPLC-UV, was introduced for the monitoring of atrazine at trace levels in environmental water samples. In the present method, benzophenone was used as a sorbent for extraction of target molecules. For dispersing solid sorbents in sample solution, very low milligram amounts of benzophenone and dispersive solvent were mixed and fast-injected into the extraction media. A cloudy solution formed, and after interaction of atrazine and the dispersed solid sorbent, the cloudy solution was centrifuged. The extracted atrazine in the solid phase was dissolved in ethanol and analyzed by HPLC-UV. The introduced method showed a low method detection limit (0.1 μg/L), good precision (relative SD: 3.9-6.9%), and appropriate relative recoveries (95-105%). Within this study, a sensitive and reliable method for the quantification of atrazine in wastewater samples was successfully developed. The obtained figures of merit for the presented sample preparation method were appropriate. The applicability of the method for analysis of atrazine in real matrices was excellent.