Test Procedure Research Articles

Efficiencies of Thermophilic Bacteria Species in Degrading Biodegradable Low-density Polyethylene Mixtures in Aquatic Ecosystems

The study examined the efficiencies of bacteria thermophiles responsible for the depolymerization of biodegradable Low-Density Polyethylene (LDPE) blends in two aquatic environments to suggest model bacteria species that could be used for reducing the accumulation of single-use LDPE in both marine and freshwater ecosystems. Each of the biodegradable LDPE, polyethylene, and cellulose was placed in respirometry jars filled with 500 mls of the freshwater and marine water respectively in a randomized design of 4 by 2 by 3 following the American Standard Testing and Materials (ASTM) procedure. To identify the bacteria species, bacterial isolation was done using pour plate and streak methods. The bacteria species were identified by morphological, biochemical, and molecular methods. The thermophilic bacteria species were confirmed by sequencing to be Bacillus cereus, Pseudomonas species among others. The results revealed that the bacteria isolates on LDPE were responsible for the biodegradation processes of the LDPE. This study concluded that Bacillus cereus and Pseudomonas species have the bioremediation potentials to break down single-use biodegradable Low-Density Polyethylene (LDPE) in aquatic environments within six (6) months.

Lumbar and Cervical Concurrent Muscle Activity Analysis During Hamstring Length Testing to Design a Customized Treatment Plan: An Observational Cross-Sectional Study.

Hamstring length plays a significant role in a spectrum of clinical entities, from injury prevention and gait dysfunction to posture correction. Evidence suggests that the prevalence of hamstring tightness (HT)/reduced length is increasing. Despite the number of available tests and treatment protocols, HT is still a functional diagnosis. This study's primary goal is to evaluate concurrent muscle (CM) usage during these testing procedures to design a unique, customized treatment protocol. The study was conducted in two stages. In phase 1, Active Straight Leg Raise (ASLR), Active Total Knee Extension (ATKE), and Active Seated Total Knee Extension (ASTKE) were carried out. Next, two pressure gauges (PGs) were placed to align with the natural lumbar and cervical curvatures while testing ASLR and ATKE. After analyzing the results for pressure gauge placement, phase 2 data were collected for tests ASLR and ATKE with PG. The results of ASLR and ATKE, both with and without PG, indicate a high prevalence rate, whereas the results of ASTKE show no prevalence. Changes in the PG values indicate CM usage. Dichotomization revealed that participants with normal test scores (non-HT group) had increased usage of CM work. Positive and negative changes in PG indicate the involved CM group. In regular practice, most healthcare professionals and fitness trainers prefer ASTKE due to the ease of the testing procedure. Directing co-professionals on their choice of tests is challenging, whereas providing knowledge about CM use paves the way for creating customized treatment plans.

Accommodative Strategies and School Administrator’s Managerial Effectiveness in Public Secondary Schools in Akwa Ibom South-South Senatorial District

This study investigated the relationship between Accommodative Strategies and School Administrator’s Managerial effectiveness in Public Secondary Schools in Akwa Ibom South-south Senatorial district. In order to achieve the purpose of this study three purposes, three research questions were raise and three null hypotheses were formulated to guide the study. The sub-variables of the study which were self-regulation, emotional adjustment, and communication adjustment and administrators’ managerial effectiveness were reviewed under conceptual framework and empirical studies, the sample size consisted of 77 respondents representing the population of 95 drawn using simple random sampling technique. Data were collected using a structured closed ended questionnaire title, Accommodative Strategies Questionnaire (ASQ) and Administrators Managerial Effectiveness Questionnaire (AMEQ). The reliability of the instrument was established by using the data from trial testing procedure which yielded .76, which showed that the instrument was reliable to be used in the study. Research questions and hypotheses were answered using PPMC (Pearson Product Moment Correlation) analyses at .05 level of significant. The findings of the study revealed that accommodative strategies such as self-regulation, emotional adjustment and communication adjustment relate significantly to the school administrators’ managerial effectiveness in secondary school students in Akwa Ibom South-South senatorial district. The findings of this study would contribute to knowledge in the sense that, researchers in different places and times would likely used as reference document conducted further studies on related issue.

Adding experimental treatment arms to multi-arm multi-stage platform trials in progress.

Multi-arm multi-stage (MAMS) platform trials efficiently compare several treatments with a common control arm. Crucially MAMS designs allow for adjustment for multiplicity if required. If for example, the active treatment arms in a clinical trial relate to different dose levels or different routes of administration of a drug, the strict control of the family-wise error rate (FWER) is paramount. Suppose a further treatment becomes available, it is desirable to add this to the trial already in progress; to access both the practical and statistical benefits of the MAMS design. In any setting where control of the error rate is required, we must add corresponding hypotheses without compromising the validity of the testing procedure.To strongly control the FWER, MAMS designs use pre-planned decision rules that determine the recruitment of the next stage of the trial based on the available data. The addition of a treatment arm presents an unplanned change to the design that we must account for in the testing procedure. We demonstrate the use of the conditional error approach to add hypotheses to any testing procedure that strongly controls the FWER. We use this framework to add treatments to a MAMS trial in progress. Simulations illustrate the possible characteristics of such procedures.

Familywise error for multiple time-to-event endpoints in a group sequential design.

We investigate the familywise error rate (FWER) for time-to-event endpoints evaluated using a group sequential design with a hierarchical testing procedure for secondary endpoints. We show that, in this setup, the correlation between the log-rank test statistics at interim and at end of study is not congruent with the canonical correlation derived for normal-distributed endpoints. We show, both theoretically and by simulation, that the correlation also depends on the level of censoring, the hazard rates of the endpoints, and the hazard ratio. To optimize operating characteristics in this complex scenario, we propose a simulation-based method to assess the FWER which, better than the alpha-spending approach, can inform the choice of critical values for testing secondary endpoints.

Study on Discharge Characteristic Performance of New Energy Electric Vehicle Batteries in Teaching Experiments of Safety Simulation under Different Operating Conditions

High-voltage heat release from batteries can cause safety issues for electric vehicles. Relevant scientific research work is carried out in the laboratory. The battery safety of laboratory experiments should not be underestimated. In order to evaluate the safety performance of batteries in the laboratory testing of driving conditions of electric vehicles, this paper simulated and compared the discharge characteristics of two common batteries (lithium iron phosphate (LFP) battery and nickel–cobalt–manganese (NCM) ternary lithium battery) in three different operating conditions. The operating conditions are the NEDC (New European Driving Cycle), WLTP (World Light Vehicle Test Procedure) and CLTC-P (China light vehicle test cycle) for normal driving of electric vehicles. LFP batteries have a higher maximum voltage and lower minimum voltage under the same initial voltage conditions, with a maximum voltage difference variation of 11 V. The maximum current of WLTP is significantly higher than NEDC and CLTC-P operating conditions (>20 A). Low current discharge conditions should be emulated in teaching simulation and experiments for safety reasons. The simulation data showed that the LFP battery had good performance in maintaining the voltage plateau and discharge voltage stability, while the NCM battery had excellent energy density and long-term endurance.

Development of a Simple Setup to Measure Shielding Effectiveness at Microwave Frequencies.

Testing the shielding effectiveness of materials is a key step for many applications, from the industrial to the biomedical field. This task is very relevant for high-sensitivity sensors, whose performance can be greatly affected by electromagnetic fields. However, the available testing procedures often require expensive, bulky, and heavy measurement chambers. In this paper, a cost-effective and reliable measurement procedure for testing the shielding effectiveness of materials is proposed. It exploits a lab-scale anechoic shielded chamber, which is lightweight, compact, and cost-effective if compared to the available commercial solutions. The measurement procedure employs a vector network analyzer to allow an accurate and fast characterization setup. The chamber realization phases and the measurement procedure are described. The shielding capability of the chamber is measured up to 26 GHz, whereas the performance of commercial shielding coatings is tested to demonstrate the measurement's effectiveness.

Development of a novel phantom for tau PET imaging

PurposeThe cortical uptake of tau positron emission tomography (PET) tracers corresponds to the Braak stage and reflects the distribution and progression of tau neurofibrillary tangles. The present study aimed to develop and validate the basic performance of a novel tau PET phantom, as well as to establish standard test procedures and analytical methods. MethodsThe tau PET phantom consisted of a brain simulation section simulated medial temporal lobe region and resolution and uniformity sections. The brain simulation section and hot rods and uniformity section contained 4 and 2 kBq/mL of 18F, respectively and images were acquired three times for 20 min with a PET/CT scanner. The resolution section was visually assessed with two sets of hot and cold rods. Recovery coefficients (RCs) as a quantitative value and coefficient of variation (CV) as image noise were determined based on the brain simulation and the uniformity section, respectively. ResultsPreparation of activity in the phantom was repeatable among three measurements. The quality of images in the brain simulation and uniformity section with the rods was good. The 5- or 6-mm rods were detected separately. The mean RCs calculated based on the VOI template were between 0.75 and 0.83. The CV at the center slice of uniformity section was 5.54%. ConclusionsWe developed a novel tau PET phantom to assess quantitative value, image noise, and detectability and resolution from brain simulation section, uniformity section, and rods, respectively. This phantom will contribute to the standardization and harmonization of tau PET imaging.

Do lower limb kinetic and kinematic differences in drop jump landings persist when players have returned to sport following knee ligamentous injuries?

Knee injuries (KI) are identified as one of the most burdensome lower limb injuries in football with long and complicated rehabilitation and high re-injury risk. Current research often describes technique deficits being present upon RTS, often being a result of poor return to sport (RTS) testing procedures. More recently, research has advocated the use of single leg vertical jump assessments, due to the specificity of the movement to injury mechanisms and the ability to consider different phases of the task to inform practitioners of joint contributions to the propulsion and landing phases. The aim of this research is to identify whether these functional deficits exist 12-24 months post RTS authorisation, to help inform future RTS in applied practice. Twenty-four semi-professional male footballers (mean ± SD: age 25 ± 4 years; stature 182 ± 7 cm; body mass 80 ± 8 kg) were recruited into a previously injured and a control group. Participants completed 5 repetitions of single leg drop jump (SLDJ). Jump height, ground contact time, reactive strength index, peak moments, angles and power contributions for the hip, knee and ankle were measured using Qualisys Track Manager then exported to Visual 3D. This study was approved by the Edge Hill University Ethics Committee. No significant findings were identified for landing one however, during the propulsion phase there was a difference between groups (P = 0.026, d=1.14) for knee flexion at take-off. Most differences were identified during landing two bilaterally in both groups. Within the injured group, differences were identified for peak hip (P = 0.011, d = 0.028) and knee powers (P < 0.001, d = 1.269), hip (P < 0.001, d = 1.924) and knee power contribution % (P < 0.001, d = 1.269). No differences were identified for the performance measures or through performing statistical parametric mapping for hip and knee powers for all three phases. Joint coupling analysis was conducted for the total ground contact time amalgamating the landing and propulsion phase, this was analysed as Hip-Knee, Hip-Ankle and Knee-Ankle, with significant findings in Hip-Knee and Hip-ankle. Both groups elicited bilateral differences however, no differences between the injured limb and dominant control limb were identified. To better apply this form of analysis to RTS testing, functional movements such should be undertaken when athletes are injury free to provide baseline measurements to refer to if undertaking rehabilitation from KI. Likewise, the current study identified that joint contributions could be identified in a more applied setting using the range of motion data from each joint.

The physical capabilities of national league female American Flag Football players

Flag Football is the fastest growing format of American Football and is a recent addition to the 2028 Olympics. The Flag format is a non-contact version of American Football, where tackles are made by removing flags from players hips. Activity profiles in the NFL (Sanchez et al., 2013, App Sci, 13:9278; Wellman et al., 2016, J Strength Cond Res, 30, 11–19) have highlighted the need for high levels of strength, power, and speed. Given the similarities within Flag, these physical capabilities may be equally applicable to Flag players. However, a lack of literature on the Flag format, and the paucity of research in female football, irrespective of format, exists. Therefore, the aim of this study was to explore the physical fitness capabilities of a national league Women’s Flag Football team. Fourteen participants (age 28 ± 5 years; stature 166 ± 8 cm; mass 79 ± 29 kg) from a national league Women’s Flag team took part in a physical fitness testing battery comprising; countermovement jump (CMJ), squat jump (SJ), broad jump (BJ), 20-yard sprint and shuttle, and the Isometric mid-thigh pull (IMTP). Participants were familiarised with testing procedures and initially completed a standardised warm-up. Mean and SD results from the jump assessments were as follows; CMJ jump height 25.1 ± 5.9 cm, peak power 39.1 ± 7.1 w/kg; SJ jump height 24.6 ± 6.3 cm, peak power 39.3 ± 6.6 w/kg; broad jump 1.89 ± 0.28 m. Absolute and relative peak force in the IMTP was 2201 ± 422 N and 30.1 ± 4.1 N/kg respectively. The Dynamic Strength Index (DSI) calculated from CMJ and IMTP peak force was 0.76 ± 0.12. Lastly, 20-yard sprint and shuttle times were 3.43 ± 0.28 and 5.36 ± 0.38 s respectively. Significant correlations were found between mean CMJ jump height and mean sprint time (p 0.001; r = −0.780), between mean CMJ jump height and mean shuttle time (p <.001; r −0.857), between mean BJ distance and mean shuttle time (p <0.01; r = 0.870) and between mean BJ distance and CMJ jump height (p <.001; r = −0.911). This study is the first of its kind to report the physical capabilities of female flag football players. The findings of this study may help develop the understanding of a growing and soon to be Olympic sport.

Spatial-sign-based high-dimensional white noises test

In this study, we explore the problem of hypothesis testing for white noise in high-dimensional settings, where the dimension of the random vector may exceed the sample sizes. We introduce a test procedure based on spatial-sign for high-dimensional white noise testing. This new spatial-sign-based test statistic is designed to emulate the test statistic proposed by Paindaveine and Verdebout [(2016). On high-dimensional sign tests. Bernoulli, 22(3), 1745–1769.], but under a more generalized scatter matrix assumption. We establish the asymptotic null distribution and provide the asymptotic relative efficiency of our test in comparison with the test proposed by Feng et al. [(2022). Testing for high-dimensional white noise. arXiv:2211.02964.] under certain specific alternative hypotheses. Simulation studies further validate the efficiency and robustness of our test, particularly for heavy-tailed distributions.

Fractal Tent Map with Application to Surrogate Testing

Discrete chaotic maps are a mathematical basis for many useful applications. One of the most common is chaos-based pseudorandom number generators (PRNGs), which should be computationally cheap and controllable and possess necessary statistical properties, such as mixing and diffusion. However, chaotic PRNGs have several known shortcomings, e.g., being prone to chaos degeneration, falling in short periods, and having a relatively narrow parameter range. Therefore, it is reasonable to design novel simple chaotic maps to overcome these drawbacks. In this study, we propose a novel fractal chaotic tent map, which is a generalization of the well-known tent map with a fractal function introduced into the right-hand side. We construct and investigate a PRNG based on the proposed map, showing its high level of randomness by applying the NIST statistical test suite. The application of the proposed PRNG to the task of generating surrogate data and a surrogate testing procedure is shown. The experimental results demonstrate that our approach possesses superior accuracy in surrogate testing across three distinct signal types—linear, chaotic, and biological signals—compared to the MATLAB built-in randn() function and PRNGs based on the logistic map and the conventional tent map. Along with surrogate testing, the proposed fractal tent map can be efficiently used in chaos-based communications and data encryption tasks.

Assessing the ability of the Cancer and Aging Research Group tool to predict chemotherapy toxicity in older Japanese patients: A prospective observational study

IntroductionThe Cancer and Aging Research Group (CARG) prediction tool was designed in the United States to predict grade ≥ 3 chemotherapy-related adverse events (CRAE) in older patients. However, its usefulness among Japanese people, who have different sensitivities to anticancer drugs and life expectancy, remains unknown. We aimed to prospectively evaluate the utility of the CARG tool for predicting severe CRAE in older Japanese patients with cancer. Material and MethodsPatients with solid tumors aged 65 years and older who commenced anticancer drug regimens from April 2018 to October 2020 were divided into three groups (low, medium, and high-risk) based on their CARG risk scores. Toxicity was prospectively observed by a pharmacist. The primary objective was to evaluate the correlation between the incidence of grade ≥ 3 CRAE and the CARG risk score. The secondary objective was to evaluate hematological and non-hematological toxicities. CRAE incidence was compared among the three groups using a closed testing procedure: (1) Cochran–Armitage test for trend and (2) chi-square test for paired comparison. ResultsThe patients (N = 165) had a median age of 71 years (range: 65–89 years). CRAE in patients divided into low-, medium-, and high-risk groups, based on CARG risk scores, were 39%, 55%, and 82%, respectively (low vs high; p < 0.001, medium vs high; p < 0.01). The incidence of severe hematologic toxicity was 37%, 35%, and 50% in the low-, medium-, and high-risk groups, respectively; the incidence of severe non-hematologic toxicity was 15%, 36%, and 65%, respectively (low vs medium; p < 0.01, low vs high; p < 0.001, and medium vs high; p < 0.01). DiscussionTo our knowledge, this is the first prospective observational study to validate the CARG prediction tool in older Japanese patients with cancer. The CARG risk score may be effective in predicting the development of non-hematologic toxicities. These results should be considered when administering chemotherapy to older Japanese patients with advanced solid tumors.

Development of sleepiness in professional truck drivers: Real-road testing for driver drowsiness and attention warning (DDAW) system evaluation.

All new vehicle types within the European Union must now be equipped with a driver drowsiness and attention warning system starting from 2022. The specific requirements for the test procedure necessary for type approval are defined in the Annex of EU Regulation C/2021/2639. The objectives of this study were to: (i) investigate how sleepiness develops in professional truck drivers under real-road driving conditions; and (ii) assess the feasibility of a test procedure for validating driver drowsiness and attention warning systems according to the EU regulation. Twenty-four professional truck drivers participated in the test. They drove for 180 km on a dual-lane motorway, first during daytime after a normal night's sleep and then at nighttime after being awake since early morning. The results showed higher sleepiness levels during nighttime driving compared with daytime, with a faster increase in sleepiness with distance driven, especially during the night. Psychomotor vigilance task results corroborated these findings. From a driver drowsiness and attention warning testing perspective, the study design with sleep-deprived drivers at night was successful in inducing the targeted sleepiness level of a Karolinska Sleepiness Scale score of ≥ 8. Many drivers who reported a Karolinska Sleepiness Scale ≥ 8 during the drives also acknowledged feeling sleepy in the post-drive questionnaire. Reaching high levels of sleepiness on real roads during daytime is more problematic, not the least from legal and ethical perspectives as higher traffic densities during the daytime lead to increased risks.

A PROPOSED METHOD FOR SPEECH AND LANGUAGE TESTING WITHIN A HEARING SCREENING PROGRAM FOR CHILDREN STARTING SCHOOL

This article sets out a proposal for how one might expand hearing screening in school children so as to include testing of speech mastery. From 2008 to 2019, the Institute of Physiology and Pathology of Hearing (IPPH) implemented a program to screen the hearing of Polish children starting school, covering over 1 million children. Its success prompted the development of a screening model to cover the entire process of verbal communication. One result was the "Equal Start in Education", a program implemented from 2019 to 2022 in the Lublin province, otherwise known as the "Lublin Program for Early Detection and Therapy of Communication Disorders in Children Starting School". It was conducted by the University Children’s Clinical Hospital in Lublin and the Maria Curie-Sklodowska University, in cooperation with IPPH, and was designed to assess hearing, auditory processing, voice, and speech-language. The program was successful in addressing various issues related to the screening of all communication skills. Here we present findings from the program and propose a testing procedure used to evaluate speech. The screening procedure might be applied more widely in the future, and could diagnose those children requiring audiological and speech-language therapy.

Functional repeated measures analysis of variance and its application

This paper is inspired by medical studies in which the same patients with multiple sclerosis are examined at several successive visits (doctor’s appointments) and described by fractional anisotropy tract profiles, which can be represented as f unctions. Since the observations for each patient are dependent random processes, they follow a repeated measures design for functional data. To compare the results for different visits, we thus consider functional repeated measures analysis of variance. For this purpose, a pointwise test statistic is constructed by adapting the classical test statistic for one-way repeated measures analysis of variance to the functional data framework. By integrating and taking the supremum of the pointwise test statistic, we create two global test statistics. In addition to verifying the general null hypothesis of the equality of mean functions corresponding to different objects, we also propose a simple method for post hoc analysis. We illustrate the finite sample properties of permutation and bootstrap testing procedures in an extensive simulation study. Finally, we analyze a real data example in detail. All methods are implemented in the R package rmfanova, available on CRAN.

Brake Wear and Airborne Particle Mass Emissions from Passenger Car Brakes in Dynamometer Experiments Based on the Worldwide Harmonized Light-Duty Vehicle Test Procedure Brake Cycle

Brake wear particles, as the major component of non-exhaust particulate matter, are known to have different emissions, depending on the type of brake assembly and the specifications of the vehicle. In this study, brake wear and wear particle mass emissions were measured under realistic vehicle driving and full friction braking conditions using current commercial genuine brake assemblies. Although there were no significant differences in either PM10 or PM2.5 emissions between the different cooling air flow rates, brake wear decreased and ultrafine particle (PM0.12) emissions increased with the increase in the cooling air flow rate. Particle mass measurements were collected on filter media, allowing chemical composition analysis to identify the source of brake wear particle mass emissions. The iron concentration in the brake wear particles indicated that the main contribution was derived from disc wear. Using a systematic approach that measured brake wear and wear particle emissions, this study was able to characterize correlations with elemental compositions in brake friction materials, adding to our understanding of the mechanical phenomena of brake wear and wear particle emissions.

Proposal of Testing Procedure for Resonance and Ferroresonance Inception Possibility in Instrument Transformers

This paper deals with the possibility of ferroresonance occurence in the interaction between circuit breakers and inductive instrument transformers. Existing standards lack guidance on testing for ferroresonant behaviour. The paper proposes a standardized testing procedure and presents measurements on a full-scale system. EMTP simulations complement the measurements for a broader network topology analysis, i.e. circuit breaker capacitance combinations. EMTP simulations are validated for a 170 kV voltage transformer and a combined instrument transformer, showing accuracy within 10%. The paper also extends the EMTP modelling application to a 420 kV voltage power transformer during design phase, ensuring it doesn't experience ferroresonance. This study offers a practical approach for testing and simulating ferroresonance in inductive instrument transformers, contributing to the safe operation of power networks.

Strength of Composite Pressure Insulators for High Voltage Circuit Breakers: An Experimental and Numerical Investigation.

Glass fiber-reinforced composite cylinders, capable of withstanding internal pressure generated during service, are increasingly utilized as insulators in high voltage circuit breakers. Different testing procedures have been suggested by various standards to assess the pressure resistance of these components. Due to its simplicity and cost-effectiveness, the split-disk testing method is the most widely used for evaluating the hoop strength of pressure cylinders during the development and verification phases. However, the method presents several aspects, such as those related to the influence of specimen geometry and friction, which require further examination since they may impact the outcome of the experimental tests. The investigation, carried out by a combination of experimental testing and finite element analyses, shows that the friction between the specimen and the semi-disks has a noteworthy effect on the hoop load applied to the specimen. Almost constant load distributions along the hoop direction, representative of the real operating conditions in a pressurized cylinder, can be achieved via proper lubrication of the contact surfaces. Furthermore, FE analyses demonstrate that the notch geometry suggested by specific standards (short notch) is not capable of inducing a uniform strain distribution in the notched region. A different notch geometry (long notch) is proposed in the study to attain a more uniform strain field over the reduced area region. The experimental results indicate that the strength measured on the short notch specimens is higher than that determined on the long notch specimens, thus confirming the significant influence of strain distribution on the strength properties measured with the split-disk method.

A Microfluidic Chip-Based Automated System for Whole-Course Monitoring the Drug Responses of Organoids.

Tumor patients-derived organoids, as a promising preclinical prediction model, have been utilized to evaluate ex vivo drug responses for formulating optimal therapeutic strategies. Detecting adenosine triphosphate (ATP) has been widely used in existing organoid-based drug response tests. However, all commercial ATP detection kits containing the cell lysis procedure can only be applied for single time point ATP detection, resulting in the neglect of dynamic ATP variations in living cells. Meanwhile, due to the limited number of viable organoids from a single patient, it is impractical to exhaustively test all potential time points in search of optimal ones. In this work, a multifunctional microfluidic chip was developed to perform all procedures of organoid-based drug response tests, including establishment, culturing, drug treatment, and ATP monitoring of organoids. An ATP sensor was developed to facilitate the first successful attempt on whole-course monitoring the growth status of fragile organoids. To realize a clinically applicable automatic system for the drug testing of lung cancer, a microfluidic chip based automated system was developed to perform entire organoid-based drug response test, bridging the gap between laboratorial manipulation and clinical practices, as it outperformed previous methods by improving data repeatability, eliminating human error/sample loss, and more importantly, providing a more accurate and comprehensive evaluation of drug effects.