Probiotic Preparation Research Articles

Probiotic drug based on Bacillus Amyloliquefaciens as an alternative to antimicrobial drugs in broiler chicken diets

This article reflects on the issue of adequate poultry feeding and the case of refusing antimicrobial drugs when growing broiler chickens. Antimicrobials use in poultry diets is directly related to the actual problem of our time - antibiotic resistance. Many antibiotics, actively used in poultry farming before, gradually lose their effectiveness - bacteria begin to suppress their action and even completely neutralize the medicine. Diseases start to occur in a more acute form. The theoretical and practical significance of the study is based on the rejection of antimicrobial drugs when a probiotic pill with antagonistic properties to pathogenic microorganisms is added to the diet of broiler chickens. The article presents the key points of the study and the results of laboratory and practical experiments to identify the antagonistic activity of a probiotic preparation based on bacillus amyloliquefaciens to pathogenic microorganisms. The research methods on which this article was based are laboratory and practical experiments, scientific and economic experiments, and statistical data analysis. The first stage of research is laboratory tests confirming the drug’s antagonistic activity and the active substance’s survival when passing through the acidic environment of the bird’s stomach. The second research stage was a scientific and economic experiment on a small number of broiler chickens. The authors proved the antagonistic activity of the probiotic preparation based on bacillus amyloliquefaciens based on the results of this study. In addition, the authors confirmed the possibility of using the drug in the diets of broiler chickens as an alternative to antimicrobials.

Protective effect of crop by-products on Lactobacillus gasseri H87 during freeze-drying and storage

Appropriate cryoprotectants are critical components of effective probiotic preparations. Beneficial lactic acid bacteria can prevent or treat genitourinary tract infections. Here, we aimed to determine a suitable cryoprotectant combined with crop by-products to ensure the viability and pathogen suppression activity of Lactobacillus gasseri H87 for vaginal capsules that can help maintain the balance of vaginal microecology. Among the five selected cryoprotectants, 15% skim milk (SM) makes L. gasseri H87 survival rate of reaches 56.5%, effectively guaranteeing maximum bacteriocin production by minimizing bacterial cell damage. On the basis of 15% SM, further addition of 1% pomegranate peel extract (PPE) optimized the production of bacteriocin, resulting in the best bacteriostatic effect. After six months of lyophilized storage, L. gasseri H87 from vaginal capsules preserved with 15% SM + 1% PPE had an inhibitory zone of 16.45 mm against the indicator strain Lactobacillus iners. Both lyophilization and storage reduced the adhesion of L. gasseri H87, but the first subculture after 6 months fully restored adhesion under all conditions. These results suggest that skim milk combined with PPE can serve as a suitable cryoprotectant. Lyophilized L. gasseri H87 vaginal capsules have the potential for use in the pharmaceutical industry.

Biochemical and Genomic Characterization of Two New Strains of Lacticaseibacillus paracasei Isolated from the Traditional Corn-Based Beverage of South Africa, Mahewu, and Their Comparison with Strains Isolated from Kefir Grains.

Lacticaseibacillus paracasei (formerly Lactobacillus paracasei) is a nomadic lactic acid bacterium (LAB) that inhabits a wide variety of ecological niches, from fermented foodstuffs to host-associated microenvironments. Many of the isolated L. paracasei strains have been used as single-strain probiotics or as part of a symbiotic consortium within formulations. The present study contributes to the exploration of different strains of L. paracasei derived from non-conventional isolation sources-the South African traditional fermented drink mahewu (strains MA2 and MA3) and kefir grains (strains KF1 and ABK). The performed microbiological, biochemical and genomic comparative analyses of the studied strains demonstrated correlation between properties of the strains and their isolation source, which suggests the presence of at least partial strain adaptation to the isolation environments. Additionally, for the studied strains, antagonistic activities against common pathogens and against each other were observed, and the ability to release bioactive peptides with antioxidant and angiotensin I-converting enzyme inhibitory (ACE-I) properties during milk fermentation was investigated. The obtained results may be useful for a deeper understanding of the nomadic lifestyle of L. paracasei and for the development of new starter cultures and probiotic preparations based on this LAB in the future.

The effect of alginate concentration on the viability of probiotics (Bacillus subtilis) in synbiotic microcapsule beads after incubation at 70°C

High temperatures in feed molding machines can cause a decline in probiotic populations in fish feed. Adding alginate in probiotic preparations is expected to wrap probiotics so that when added to the feed before pelleting, it does not experience a drastic decrease. This study investigated the decline in probiotic populations in fish feed caused by high temperatures in feed molding machines and evaluated the use of alginate in probiotic preparations to prevent drastic decreases in viability. The study aimed to determine the viability of Bacillus sp. in synbiotic microcapsule beads with varying levels of alginate concentration after exposure to 70°C. The research used four treatments and three replicates with a randomized complete design. The results showed that the alginate concentration affected the diameter of the beads of synbiotic microcapsules, but it did not significantly affect the viability of Bacillus sp. The viability of probiotics in alginate beads after exposure to 70°C was 5.182±0.527 - 5.720±0.072 log cfu/ml. Therefore, using alginate in probiotic preparations can be considered as a reference for adding probiotics to fish feed before pelleting to maintain the viability of the probiotics in high-temperature conditions.

Probiotics and their application in aquaculture for improving the growth and immunity of fish

To increase the digestibility and digestibility of feed, stimulate the growth and development of animals, and increase nonspecific immunity, enzyme, probiotic, prebiotic and combined enzyme-probiotic preparations, as well as complex probiotic preparations enriched with phytocomponents are used. Probiotics for artificially grown fish are beneficial bacteria and live microbiological additives that are traditionally added to agricultural aquaculture systems to maintain health, productivity and growth. Therefore, a promising direction in aquaculture is the use of feed with probiotic cultures. The use of probiotics is associated with solving various health problems, in particular, with increasing the efficiency of digestion and stimulating growth and development. Probiotics, having a positive effect on the host’s body, contribute to the restoration of digestion, biological status, and immune response and increase the effectiveness of vaccination. The use of probiotics significantly reduces the cost of treating diseases in animals, increases productivity and improves product quality. In recent years, many studies have been conducted on the use of various probiotics in fishing. This review is aimed at generalizing the topic of the possible use of gram-positive bacteria as probiotics in a comprehensive study of the existing literature. With the expansion of ideas about the biological effectiveness of probiotics and the proof that the structural elements of cells and their metabolites are at least effective in some cases. As a result, various types of bacteria, including bacilli, lactic acid bacteria, and microbes, were used to improve the growth and immunity of various fish. However, this study conducted a review of the scientific literature showing that probiotics in aquaculture have a positive effect on the overall productivity of fish.

Ecologization of the fertilizer system of agricultural cultures through the use of a mixture of ground water and probiotic preparations

Ecologization of the fertilizer system of agricultural cultures through the use of a mixture of ground water and probiotic preparations

Effects of the multi-strain probiotic preparation LabMix on some immune indices and intestinal microbiota in an antibiotic associated diarrhea rat model

Effects of the multi-strain probiotic preparation LabMix on some immune indices and intestinal microbiota in an antibiotic associated diarrhea rat model

ВЛИЯНИЕ ПАРАЗИТАРНЫХ ПРОСТЕЙШИХ НА КИШЕЧНУЮ МИКРОБИОТУ ЭКСПЕРИМЕНТАЛЬНЫХ ЖИВОТНЫХ

There is relatively little information on the effect of probiotic supplements used in helminthiasis, and one can only guess how much probiotics can affect helminthiasis and associated inflammatory and immunopathological changes in the gastrointestinal tract, as well as how parasitosis can affect the properties of probiotic preparations. The aim of this study was to study the effect of Giardia and Cryptosporidium on the intestinal microbiota of experimental animals in the study of probiotics. In the intestinal microbiota of experimental mice with successfully integrated probiotic strains of Lactobacillus sakei and Lactococcus lactis, cryptosporidium and giardia, which may indicate the antagonistic effect of cryptosporidium and giardia did not find these strains after protozoal invasion against these strains. Key words: probiotics, cryptosporidium, giardia, microbiome.

Investigation of the effect of probiotic strains on the productivity of laying hens

The aim of the study was to study the effect of diets using the potentially probiotic drug Bacillus amyloliquefaciens KB54 as part of compound feed (the rate of administration is 0.1% of the total diet, the content of viable spores in the final product is 106CFU/g) in the diets of laying hens of the Dekalb cross. In the course of the study, it was established that the viability indicators of laying hens were at a high level and the safety of livestock in the studied groups during the period of the experiment was 100%; egg production in the experimental group was 96.7%, which is 2.3% more than in the control group of laying hens; chickens in the experimental group exceeded the control in growth intensity, which affected an increase in live weight by 12.5%, or 188.5 grams, which is 1.7 percentage points or 25.2 grams more than in the control group; feed costs for 10 eggs and 1 kg of egg mass in the experimental group were 1.43 and 2.36 kg, respectively, which is 11.7 and 14.5% lower than the control values. In this regard, we recommend using the probiotic preparation Bacillus amyloliquefaciens KB54 in the diets of laying hens, introduced into the compound feed in the amount of 0.1% of the total diet with the content of viable spores in the final product 106CFU/g.

Effect of a Probiotic Preparation Based on Lactic and Propionic Acid Bacteria on the Growth of Young Rainbow Trout Oncorhynchus Mykiss in Aquaculture

Effect of a Probiotic Preparation Based on Lactic and Propionic Acid Bacteria on the Growth of Young Rainbow Trout Oncorhynchus Mykiss in Aquaculture

ИСПОЛЬЗОВАНИЕ НАУПЛИЙ РАЧКОВ ARTEMIA SALINA В КАЧЕСТВЕ НОСИТЕЛЯ ПРОБИОТИКОВ БАКТЕРИЙ РОДА BACILLUS В АКВАКУЛЬТУРЕ

This paper presents the results of the investigation of the effect of probiotic preparations based on Bacillus strains on Artemia salina nauplii. The use of live food is a necessary step in cultivation of many fish species, and Artemia nauplii are widely used for this purpose. It is crucial to use probiotics in aquaculture, as they can act as natural antagonists of aquatic pathogens, as well as stimulate fish growth and development. This work aims to study the immobilization of probiotic Bacillus strains on Artemia nauplii in order to develop highly effective feeds with probiotic properties for juvenile fish. Experiments on the introduction of various doses of preparations based on three probiotic strains of Bacillus into the water during the rearing of Artemia salina cysts were carried out. The number of bacteria in the water, as well as on the surface and inside the nauplii, was estimated by plating on nutrient media. It has been established that, despite the fact that brine shrimps do not feed at the orthonauplius stage, when probiotic strains are introduced into the water, bacteria are immobilized on the covers of the crustaceans; the concentration of the introduced Bacillus strains in the wipe samples taken from the nauplii surface is 1 order higher than it is for the planktonic form in the water. The introduction of bacilli at a dose of 0.0017 g/L activated the release of nauplii from cysts by 9-41 % of the control, while increasing the dose to 0.17 g/L led to a decrease in the proportion of hatched crustaceans. Thus, if the correct dosage were identified, it would make it possible to accomplish two important tasks at once: to obtain the live feed with probiotic properties for fish larvae and to increase the yield of nauplii.

INFLUENCE OF PROBIOTICS ON THE MESOTHELIN LEVEL IN WOMEN WITH ENDOMETRIOSIS ASSOCIATED WITH INFERTILITY IN COMPLEX PREPARATION FOR ASSISTED REPRODUCTIVE TECHNOLOGIES.

The aim: To study the determination of Mesothelin level in women with endometriosis associated with infertility and estimate influence of probiotic on endometriosis according of Mesothelin level in complex preparation before assisted reproductive technologies. Materials and methods: In this study, we conducted a retrospective analysis of the medical records of 40 infertile women who underwent assisted reproductive technologies while also using the probiotic "Femina Probiz." We divided the participants into two groups. The control group comprised 11 women who had tubal infertility due to a previous inflammatory condition but were otherwise found to be in good health through comprehensive clinical and laboratory assessments. These women, aged between 21 and 42 with an average age of 29.75 years, did not use the probiotic "Femina Probiz." The main group consisted of 29 women diagnosed with external genital endometriosis who were undergoing assisted reproductive technologies. Women in the main group received the probiotic "Femina Probiz" from Unic Biotech Ltd, India. They took one tablet twice a day for one month as part of their overall treatment before undergoing assisted reproductive technologies. We measured the Mesothelin levels before and after this preparation phase. This study was conducted at Bukovinian State Medical University and Centre of Reproductive Medicine. It's worth noting that the primary infertility incidence was significantly higher in the main group of patients. Results: In the main group, we observed that the Mesothelin level was 0.73±0.01, which was significantly higher than the post-preparation level (0.59±0.01). In contrast, the control group had a Mesothelin level of 0.49±0.01. Interestingly, we noted that the Mesothelin level in patients increased approximately twofold before preparation compared to those who had undergone preparation. This suggests that the use of the probiotic led to a sharp reduction in the elevated Mesothelin levels. Consequently, the significant decrease in Mesothelin levels after using the probiotic indicates its effectiveness and potential utility in the preparation phase of assisted reproductive technologies programs. Conclusions: The elevated Mesothelin levels indicate a strong association between the pathogenesis of endometriosis and inflammation, as well as damage to the peritoneum. The incorporation of a probiotic as part of a comprehensive preparation regimen prior to assisted reproductive technologies notably enhances the overall health of patients and leads to a reduction in Mesothelin levels. Based on our findings, we highly recommend the inclusion of this probiotic preparation in clinical practice.

Effectiveness of using fermented probiotic supplement Cellobacterin+ on inclusion of flax and hemp cakes in ruminant diets

Cellobacterin+ is a feed additive with enzymatic activity containing a complex of natural live bacteria. It is a probiotic that improves fibre digestion, accelerates the maturation of rumen microflora and normalises the digestive system. The set task is achieved using a standard ruminant diet, in which traditional soya cake was replaced by linseed cake and hemp cake in equivalent amounts of 5 % and 10 % with the use of the enzymatic probiotic preparation “Cellobacterin+” in a dose of 10.0 g.

SURVIVAL OF PROBIOTIC LACTOBACILLI IN THE COMPOSITION OF GRANULAR FEED DURING ITS LONG-TERM STORAGE

Bacteria of the genus Lactobacillus are a promising group of microorganisms that can be used as probiotics in aquaculture. An effective method of targeted delivery of these bacteria to the body of aquatic organisms, especially in large farms, is the addition of probiotic preparations directly to the feed. However, there is a problem of long-term storage of feed with added probiotics. In this study, the survival of probiotic microorganisms of the genus Lactobacillus in the composition of granular feed during its long-term storage was evaluated. The probiotic strains of lactobacilli Lactobacillus casei IMV B-7280, L. delbrueckii subsp. bulgaricus IMV B-7281 and L. acidophilus IMV B-7279, kindly provided by the D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, were added to Aller Aqua Bronze feed samples, followed by pelletizing and drying and storage for 28 days. A gradual decrease in the number of probiotic microorganisms in all the studied feed samples during the experiment was found. Two weeks after the beginning of the study, the number of live cells of L. casei and L. bulgaricus did not differ statistically from each other and amounted to about 60% of the initial number of cells. In contrast, the number of viable L. acidophilus cells was 52%. The highest survival rate in the granular feed on the 28th day of its storage was observed for probiotic microorganisms L. delbrueckii subsp. bulgaricus IMV V-7281, which constituted 58% of the initial number of introduced bacteria. The lowest survival rate among the studied lactobacillus strains was observed for L. acidophilus. The optimal shelf life of granular feed enriched with probiotics is 14 days from the date of manufacture.

Probiotička karakterizacija soja Limosilactobacillus fermentum BGHV110 i njegov uticaj na urođeni imunski odgovor kod Caenorhabditis elegans

Probiotic lactobacilli exhibit the potential to promote health benefits for the host. Thanks to its numerous beneficial effects on human health, Limosilactobacillus fermentum stood out as an excellent candidate for the development of commercial probiotic preparations aiming to prevent community-acquired infections. In this study, several in vitro tests, including biofilm formation assay, assessment of antibiotic susceptibility, survival in simulated gastrointestinal tract conditions and attachment to intestinal Caco-2 cells, were used to estimate the safety and probiotic potential of L. fermentum BGHV110 strain. Additionally, Caenorhabditis elegans was used as an in vivo model system for the evaluation of L. fermentum BGHV110 influence on the host's innate immune response. The results revealed that L. fermentum BGHV110 strain showed an excellent capability to survive harsh conditions of the gut, to attach to intestinal Caco-2 cells and to stimulate conserved p38 MAPK immunity pathway and expression of the clc-1 claudin-like gene and antimicrobial peptides in C. elegans in order to enhance the immune response against pathogens. Finally, L. fermentum BGHV110 showed no virulence traits and susceptibility to tested antibiotics, confirming its safety status which enables it to be applied as a future probiotic.

К пониманию действия пробиотических препаратов на рост и гематологические параметры крови цыплят-бройлеров

К пониманию действия пробиотических препаратов на рост и гематологические параметры крови цыплят-бройлеров

Metabolite composition of probiotic strain lactobacillus paracasei B-11821

Lactobacillus paracasei B-11821 is a probiotic strain of microorganisms, for which previous studies have shown pronounced antagonistic and adhesive activity, a high level of acid formation and manufacturability. The characteristics of the metabolite profile of the promising probiotic strain can significantly expand the scope of its application in the medical, pharmaceutical and food industries. Using high- performance liquid chromatomass spectrometry and gas-liquid chromatography, it was established that this strain is an active producer of essential amino acids: (isoleucine (1.920 mg/mL), phenylalanine (0.963 mg/mL), valine (0.840 mg/mL), leucine (0.561 mg/mL), methionine (0.369 mg/mL), threonine (0.233 mg/mL), histidine (0.055 mg/mL), lysine (0.008 mg/mL)); as well as a number of replaceable amino acids (tyrosine (0.622 mg/mL), alanine (0.438 mg/mL), proline (0.278 mg/mL), glycine (0.111 mg/mL), glutamine (0.015 mg/mL), asparagine (0.014 mg/mL), serine (0.008 mg/mL), arginine (0.005 mg/mL)). The strain is also an active producer of organic acids (lactic (16.2 mg/ml), acetic (0.9 mg/ml) and gamma-aminobutyric (0.3 mg/ml) GABA, propionic (0.02 mg/ml) acids. The obtained results can be used for development based on the strain Lactobacillus paracasei B-11821 probiotic preparations, functional food products, technologies for synthesis of lactic and gamma-aminobutyric acids.

Оценка эффектов стимуляции роста Bifidobacterium bifidum полисахаридами из лиственницы сибирской

Due to the rich chemical composition of Siberian larch (Larix sibirica Ledeb), its products are widely used in medicine, pharmacology, agriculture, perfumery and cosmetics industry. Larch wood and bark contain a large amount of low molecular weight substances with high biological activity, namely: essential oils, ascorbic acid, tannins, anthocyanins, flavonoids, organic acids. The most important and useful of them are flavonoids due to their significant content in raw materials such compounds as: dihydroquercetin (DHA), arabinogalactan (AG), quercetin (QU), ardixin (ARD). On the basis of dihydroquercetin, preparations for the treatment and prevention of atherosclerosis, capillary-strengthening agents, anti-allergic drugs are created, and due to the high ability to complex formation, it actively removes heavy metals, including radionuclides, from the body. The main experimentally established properties of AG are immunomodulatory and antioxidant effects. In the diet, there is often a deficiency of dietary fiber needed by the human body. Quercetin is a powerful antioxidant that increases the body's resistance to radiation and a food antioxidant. It can also enhance antiinflammatory, antiviral, and immunoprotective effects. Ardiksin is a nanobiocomposite containing no. more than 95.0% of the natural polysaccharide arabinogalactan and no. less than 5% of the bioflavonoid dihydroquercetin. The biological activity of ardixin has shown its effectiveness in the treatment of oncological diseases as a concomitant agent during radiation and chemotherapy. Based on the significant importance of these polysaccharide compounds in the creation of new functional food products and therapeutic agents for medicine, in this study, the goal was to identify the effect of stimulating the growth of the commercial probiotic preparation Bifidobacterium bifidum when cultivated on media with polysaccharide compounds: purified arabinogalactan, dihydroquercetin, quercetin, ardixin. These polysaccharide compounds are processed products of Siberian larch (Larix sibirica Ledeb). In the course of a study on the evaluation of growth stimulation of B. bifidum by polysaccharides: AG-purified, DHA, QU, ADC, it was found that: 1) growth of B. bifidum is most effective in a medium with DHA; 2) when growing on a medium with AG-clean. there is almost five times less growth stimulation compared to DHA; 3) the smallest growth of B. bifidum is observed in the medium with QU, which is more than 208 times less than its growth on the medium with DHA.

Bacillus subtilis TO-A extends the lifespan of Caenorhabditis elegans.

Clostridium butyricum TO-A, Enterococcus faecium T-110, and Bacillus subtilis TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with Escherichia coli OP50 in extending the lifespan of Caenorhabditis elegans. To compare the C. elegans lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed C. butyricum TO-A, E. faecium T-110, or B. subtilis TO-A increased by 11, 12, and 26%, respectively, compared with worms fed E. coli OP50. In addition, we conducted a metabolomic analysis of methanol extracts of B. subtilis TO-A cells, which exhibited the strongest lifespan-extending effect on C. elegans among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of C. elegans were identified in B. subtilis TO-A cells.

The improvement of treatment and prevention of non-alcoholic fatty liver disease progression against the background of metabolic disorders

Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases, with pathogenetic mechanisms involving complex factors, in particular changes in the intestinal microbiota. The development and evaluation of the effectiveness of NAFLD treatment regimens, affecting intestinal microbiota disturbances, presents an urgent issue in modern medicine. Objective — to determine effects of combined therapy, including ursodeoxycholic acid (UDCA) and probiotic preparation (strains of Lactobacillus acidophilus, L. rhamnosus, Streptococcus salivarius subsp. thermophilus, L. delbrueckii subsp. Bulgaricus) on the metabolic disorders and inflammatory processes in liver tissue of patients with NAFLD. Materials and methods. The study included 108 patients with NAFLD with metabolic disorders and 30 people of the control group, who were examined on the basis of the Department of Gastroenterology and Therapy and outpatient department of the GI «L. T. Mala National Institute of Therapy of the NAMS of Ukraine». Women prevailed in both groups. The mean age of patients was 54.6 ± 11.7 years. The patients were randomized into two groups, comparable by gender and age ratio. Both groups received non‑drug therapy. The comparison group (48 patients) received UDCA preparation only, the main group (60 patients) received additional probiotic preparation «Yogurt capsules» (Pharmascience Inc., Canada). The treatment lasted 12 weeks. Patients were examined at the beginning and end of treatment with additional determination of pro‑inflammatory markers: C‑reactive protein CRP and TNF‑α. Statistical analysis was performed with the package Statistica 13.1 using nonparametric methods. Results. Patients’ examination in the dynamics of treatment showed an improvement in NAFLD course due to a decrease in the levels of alanine aminotransferase, aspartate aminotransferase and γ‑glutamyl peptidase (ALT, AST and GGT) in both groups. Moreover, against the background of treatment, the tendency was observed to the decrease of visceral fat percentage, as well as the visceral obesity index, which in group I significantly decreased in almost 1.6 times. In contrast to the carbohydrate metabolism indicators, the dynamics of which did not differ significantly between the groups, a significant effect of the developed therapy on the lipid profile was determined. The triglycerides (TG) levels in the main group decreased 1.3 times, and LDL cholesterol 1.6 times. In addition, the significant decrease in TNF‑alpha levels by 57.51 % was observed under the influence of treatment with the addition of probiotic yogurt. Conclusions. Combined therapy which includes lifestyle modification by means of non‑drug therapy, UDCA with the additional use of a complex probiotic preparation yogurt capsules, has a pathogenetic focus and proven effectiveness in the treatment of NAFLD.