Metabolite composition of probiotic strain lactobacillus paracasei B-11821

Abstract

Lactobacillus paracasei B-11821 is a probiotic strain of microorganisms, for which previous studies have shown pronounced antagonistic and adhesive activity, a high level of acid formation and manufacturability. The characteristics of the metabolite profile of the promising probiotic strain can significantly expand the scope of its application in the medical, pharmaceutical and food industries. Using high- performance liquid chromatomass spectrometry and gas-liquid chromatography, it was established that this strain is an active producer of essential amino acids: (isoleucine (1.920 mg/mL), phenylalanine (0.963 mg/mL), valine (0.840 mg/mL), leucine (0.561 mg/mL), methionine (0.369 mg/mL), threonine (0.233 mg/mL), histidine (0.055 mg/mL), lysine (0.008 mg/mL)); as well as a number of replaceable amino acids (tyrosine (0.622 mg/mL), alanine (0.438 mg/mL), proline (0.278 mg/mL), glycine (0.111 mg/mL), glutamine (0.015 mg/mL), asparagine (0.014 mg/mL), serine (0.008 mg/mL), arginine (0.005 mg/mL)). The strain is also an active producer of organic acids (lactic (16.2 mg/ml), acetic (0.9 mg/ml) and gamma-aminobutyric (0.3 mg/ml) GABA, propionic (0.02 mg/ml) acids. The obtained results can be used for development based on the strain Lactobacillus paracasei B-11821 probiotic preparations, functional food products, technologies for synthesis of lactic and gamma-aminobutyric acids.