Probiotic Drug Research Articles

Experience in the use of probiotic drug in pig breeding

The results of research on the use of the probiotic drug “Profort” in the diet of young pigs have been presented in the article. The research was carried out under the conditions of LLC “Agrofirma Ariant” on the crossbreds of young pigs. The purpose of the research was to study the eff ectiveness of the effect of the probiotic drug “Profort” in intensive pig rearing on the reproductive traits of sows and the productive traits of piglets. In order to conduct the scientific and economic experiment at the first stage, 2 groups of pregnant sows were formed in each with 10 heads. The feeding and housing of the experimental animals were the same. In addition to the main diet the sows of the experimental group were fed the probiotic additive “Profort” at the dose of 500 g/t of feed for 30 days before and 30 days after farrowing. At the second stage the resulting young animals were divided into 3 groups. In addition to the main diet the young animals of the 1st experimental group have been received the probiotic drug “Profort” from 7 to 105 days together with compound feed. Piglets of the 2nd experimental group have been received the probiotic additive from 7 to 30 days. It has been found during of studies that as a result of the use of probiotic additive the prolificacy of sows in the experimental group was higher – 10,6 compared to 10,4 heads in the control group. The livability of piglets for weaning in the experimental group was 97,9 % compared to 93,9 % in the control group. The absolute gain in live weight of piglets from 30 to 105 days in the experimental groups was 34,94–36,98 kg, which was higher than in the control group by 7,9–14,2 %. In the blood of piglets of the experimental groups, the increase in the level of red blood cells, total protein, calcium and phosphorus has been noted.

Probiotic drug “Propionovy” in the diets of broiler chickens

Currently, probiotics are an alternative to feed antibiotics in poultry farming. Probiotic cultures that are part of these drugs suppress the development of pathogenic and contain conditionally pathogenic bacteria at a safe level, as well as maintain a healthy microflora at a normal level. The mechanism of action of probiotics is studied by specialists from different countries. The purpose of the work was to study the effect of the probiotic drug “Propionovy” on the productive traits and physiological state of broiler chickens. Experimental studies have been carried out under the conditions of LLC “Kuzbassky broiler” in the Kemerovo region. The object of the study was broiler chickens of the cross Hubbard ISA F-15. Comprehensive studies have been conducted on the use of the probiotic drug “Propionovy”, which includes strains of Propionibacterium freudenreichii spp. in the diets of broiler chickens. The influence of various dosages of the studied drug on the productivity and quality of poultry meat, on morphological and biochemical parameters of blood has been studied. The optimal dose of introducing the drug “Propionovy” into the diets of broiler chickens, which is most effective for production from the economic point of view has been determined. It has been found according to the results of the production inspection that the inclusion of the probiotic drug “Propionovy” in the diets of broiler chickens contributed to the increase in the total gain in live weight in the experimental group by 3,76 % and the decrease in the prime-cost of production by 0,45 %, which allowed to obtain an additional profit of 2,83 rubles from 1 head. It has been found that the introduction of the probiotic drug “Propionovy” into the diets of broiler chickens of the cross Hubbard ISA F-15 has the positive effect on their productive traits and physiological state, and also allows us to get additional profit.

Coronavirus infection and gut microbiota

At present time, a number of questions regarding the pathophysiological characteristics and therapeutic approaches to the treatment of the new coronavirus infection COVID-19 remain unresolved. In some cases, patients with COVID-19 may experience symptoms of gastrointestinal tract disorder. According to the literature, the new SARS-CoV-2 coronavirus can replicate in the gastrointestinal tract and may affect the gut microbiota. The article aims to review studies about the possible relationship between the gut microbiota condition and the course of COVID-19 infection, as well as to consider the gut microbiota as a potential therapeutic target and probiotic drugs as possible therapeutic agents in the treatment of viral infections, including COVID-19 infection. It is known that gut microbiota condition is one of the factors determining the susceptibility and features of the bodys response to various infectious agents, possibly including the COVID-19 infection. Currently published studies demonstrate a possible relationship between the gut microbiota condition and the course of COVID-19 infection, however, to confirm this hypothesis, additional studies are required, which will allow to make more unambiguous conclusions with subsequent development of new approaches to the prevention and treatment of infection. Potentially a lot of hope in this direction is inspired by the results of probiotics studies, which showed that their use may reduce the frequency and severity of viral infections of the upper respiratory tract. However, currently, there is insufficient data to extrapolate the results of these studies to COVID-19 patients.

Effect of probiotic drug “Mucinolextra” on the physiological state of the body of young cattle

Relevance. The work is devoted to the assessment of the effectiveness of the feed additive “Mucinol-extra” together with chitosan, on the hematological and biochemical parameters of the blood of heifers of the black-and-white breed. The goal was to study the effect of the probiotic “Mucinol-extra” on the physiological state of the body of heifers. Experimental data showed that the content of total protein, albumins and globulins increased in both experimental groups. At the same time, the total protein index in the second group increased by 10.5%. The use of the probiotic preparation “Mucinol-extra” had a positive effect on the concentration of glucose in the blood of the experimental group. The content of erythrocytes in the blood of heifers in the experimental group was 12.23% higher than in the control group. Accordingly, the hemoglobin content in the experimental group exceeded the control significantly by 11.5%. The influence of the probiotic “Mucinol-extra”, together with chitosan, promoted the stimulation of protein metabolism, an increase in the level of redox processes and an improvement in the functional state of the body of young cattle. Methods. To study the effect of the probiotic “Mucinol-extra” on hematological parameters and biochemical parameters of blood serum, 2 groups of clinically healthy heifers of the black-and-white breed of 10 days of age were formed, selected according to a single physiological state, taking into account live weight, sex, age and clinical states. Each group consisted of 10 animals. Results. It was found that feeding the heifers of the experimental groups with the probiotic preparation “MUTSINOL”-extra had a favorable effect on the hematological and biochemical parameters of the blood.

Productivity of laying hens when fed probiotic feed additive Amilocin

The possibilities of increasing the productivity of different poultry species by increasing the absorption of feed nutrients can be realized through the use of new biologically active components that are usually added to traditional feed based on live cultures of microorganisms of the normal microflora of the gastrointestinal tract. The effectiveness of different probiotic drugs varies depending on the types and strains of microorganisms that are included in their composition, the dose of the drug, the scheme of its use, age, type, physiological state and productivity of animals. The research has been carried out to study the influence of the use of the probiotic feed additive Amilocin in the diets of laying hens of the cross Hisex Brown on their productivity. The positive influence of the studied feed additive on the live weight and livability of laying hens, egg productivity, quality and quantity of eggs obtained, morphological and biochemical parameters of the blood of experimental poultry, slaughter parameters and chemical composition of the meat of experimental laying hens has been established. The optimal rate of introduction of probiotic feed additive Amilocin into the diet of laying hens has been determined (0,5 g/head/day at the beginning of egg laying during 10 days, 1,0 g/head/day at the peak of egg laying during 10 days, followed by 1 g/head/day monthly for 10 days until the end of egg laying). The economics effectiveness of using the probiotic feed additive Amilocin per 1,0 ruble of costs was 1,24– 1,35 ruble. According to the research data recommendations for the use of the probiotic feed additive Amilocin have been proposed.

A new, reliable, and high-throughput strategy to screen bacteria for antagonistic activity against Staphylococcus aureus

BackgroundAntibiotic-resistant Staphylococcus aureus clones have emerged globally over the last few decades. Probiotics have been actively studied as an alternative to antibiotics to prevent and treat S. aureus infections, but identifying new probiotic bacteria, that have antagonistic activity against S. aureus, is difficult since traditional screening strategies are time-consuming and expensive. Here, we describe a new plasmid-based method which uses highly stable plasmids to screen bacteria with antagonistic activity against S. aureus.ResultsWe have created two recombinant plasmids (pQS1 and pQS3) which carry either gfpbk or mCherry under the control of a S. aureus quorum-sensing (QS) promoter (agrP3). Using this recombinant plasmid pair, we tested 81 bacteria isolated from Holstein dairy milk to identify bacteria that had growth-inhibiting activity against S. aureus and suggest potential explanations for the growth inhibition. The stability test illustrated that pQS1 and pQS3 remained highly stable for at least 24 h in batch culture conditions without selection pressure from antibiotics. This allowed co-culturing of S. aureus with other bacteria. Using the newly developed pQS plasmids, we found commensal bacteria, isolated from raw bovine milk, which had growth-inhibiting activity (n = 13) and quorum-quenching (QQ) activity (n = 13) towards both S. aureus Sa25 (CC97) and Sa27 (CC151). The pQS-based method is efficient and effective for simultaneously screening growth-inhibiting and QQ bacteria against S. aureus on agar media.ConclusionsIt was shown that growth-inhibiting and QQ activity toward pQS plasmid transformants of S. aureus can be simultaneously monitored by observing the zone of growth inhibition and reporter protein inhibition on agar plates. Newly identified antagonistic bacteria and their functional biomolecules are promising candidates for future development of probiotic drugs and prophylactics/therapeutics for bacterial infections including S. aureus. Furthermore, this new approach can be a useful method to find bacteria that can be used to prevent and treat S. aureus infections in both humans and animals.

The effectiveness of the use of the probiotic drug Plantarum in the feeding of dairy goats

Currently dairy goat breeding is one of the most promising branches of animal husbandry. The use of probiotics in the diets of farm animals makes it possible to increase the digestibility and accessibility of feed, reduce production costs, increase productivity and livability, and also get high-quality and biologically complete food products from them. However, despite the widespread practice of using probiotics in animal husbandry, the use of probiotic drugs in dairy goat breeding remains insufficiently studied, which justifies the relevance of the topic of the work. The purpose of the research was to study the effectiveness of the use of the probiotic drug Plantarum in feeding dairy goats. Research has been carried out to study the effect of the probiotic drug Plantarum, consisting of biologically active strains of lactobacilli of the genus Lactobacillus and propionic acid bacteria of the genus Propionibacterium, on the reproductive ability, milk productivity and morphological and biochemical parameters of the blood of goats in the type of Zaanen breed, as well as on the conformation and constitutional features, live weight and hematological parameters of the off spring obtained from them (young goats). The object of the study were mature goats of dairy productivity in the type of Zaanen breed at the age of 2 years and their off spring (young goats) from birth to the age of 4 months. During the experiment the effectiveness of the use of the probiotic drug Plantarum in the diets of pregnant goats and young goats at a dose of 0,6 ml/kg of body weight/day and 0,8 ml/kg/day was proved.

Influence of probiotic on the meat productivity and indicators of the control slaughter of pigs

One of the triggers for increasing the meat productivity of pigs and improving the quality of meat is the use of probiotic feed additives in the diets of young pigs and pregnant sows. The purpose of the research was to study the increase in meat productivity of pigs when using the probiotic drug Bifidum Bug in the diet. Scientific and economic experiment on the young pigs of Duroc breed was carried out on the basis of OOO “Agrofirma Ariant” in the Emanzhelinsky area in the Chelyabinsk region in the period 2020–2022. Data on the use of the probiotic Bifidum Bug in the diet of fattening pigs are presented. Probiotic feed additive was given to animals in the amount of 0,1 mg/kg of live weight daily. As a result of the control slaughter of animals of the control and experimental groups of 3 animals from each group, the following data were obtained: the slaughter yield in pigs of the 1st control group was 63,4 %, while in pigs of the 2nd and 3rd experimental groups it was higher – 68,8 and 68,3 %, respectively. The highest ratio of edible parts of the carcass to inedible was observed in pigs in the 2nd experimental group 8,6:1, to a lesser extent in the 3rd experimental group 8,3:1, while in the 1st control group it was 8,1:1. The chemical composition of the muscle tissue of pigs in the experimental groups in the final as a result, it affected the energy value of meat. So, in the 1st control group the energy value of 100 g of meat was 182,2 kcal or 763,4 kJ, in the 2nd experimental group 189,4 and 793,6, respectively, and in the 3rd experimental group it was 186,1 kcal and 779,9 kJ.

Use of Antibiotics and Probiotics Reduces the Risk of Metachronous Gastric Cancer after Endoscopic Resection.

Simple SummaryHelicobacter pylori is the most important cause of gastric cancer, and its eradication reduces the incidence of gastric cancer after endoscopic resection. However, incidence of metachronous gastric cancer is still high. More studies are needed to identify other chemopreventive drugs that may reduce the incidence of this disease. In this study, we focused on the alteration of the intragastric microbiome and examined the association between the use of antibiotics and probiotic drugs and risk of metachronous gastric cancer. Our findings suggest that the gut microbiome is associated with metachronous gastric cancer development.Metachronous gastric cancer often occurs after endoscopic resection. Appropriate management, including chemoprevention, is required after the procedure. This study was performed to evaluate the association between medication use and the incidence of metachronous gastric cancer after endoscopic resection. This multicenter retrospective cohort study was conducted with data from nine hospital databases on patients who underwent endoscopic resection for gastric cancer between 2014 and 2019. The primary outcome was the incidence of metachronous gastric cancer. We evaluated the associations of metachronous gastric cancer occurrence with medication use and clinical factors. Hazard ratios were adjusted by age and Charlson comorbidity index scores, with and without consideration of sex, smoking status, and receipt of Helicobacter pylori eradication therapy during the study period. During a mean follow-up period of 2.55 years, 10.39% (140/1347) of all patients developed metachronous gastric cancer. The use of antibiotics other than those used for H. pylori eradication was associated with a lower incidence of metachronous gastric cancer than was non-use (adjusted hazard ratio (aHR) 0.56, 95% confidence interval (CI) 0.38–0.85, p = 0.006). Probiotic drug use was also associated with a lower incidence of metachronous gastric cancer compared with non-use (aHR 0.29, 95% CI 0.091–0.91, p = 0.034). In conclusion, the use of antibiotics and probiotic drugs was associated with a decreased risk of metachronous gastric cancer. These findings suggest that the gut microbiome is associated with metachronous gastric cancer development.

The use of probiotics for the correction of disturbances of metabolism in cows

Currently, there is a huge range of different feed additives on the market, which are widely used in animal husbandry, but they act simultaneously, i. e. by stopping their use the resulting effect is also lost. Metabolic disorders due to feeding errors begin imperceptibly without any precursors, and first of all this affects the biochemical parameters of the blood only later leads to alimentary diseases with deep often irreversible degenerative changes in organs and tissues. In practice, there are rarely disorders of any one type of metabolism more often there are combinations of various metabolic disorders, especially often this occurs in the absence of a complete balanced feeding. The purpose of the work was to evaluate the effectiveness of the use of a probiotic drug based on Bacillus subtilis in the watering of lactating cows for the correction of metabolic disorders. In order to assess the actual presence of probiotics in the water during the experiment its analysis has been carried out. It has shown that in the water that enters the distribution tank the total microbial number was 9×10[sup]5[/sup] CFU/ml, and in the drinker this indicator was at the level of 3,8×10[sup]5[/sup] CFU/ml. Common coliform bacteria and thermotolerant coliform bacteria have been not detected. Studies have shown that when using the drug based on Bacillus subtilis containing five strains of bacteria in an amount of 0,01 %, which is 6 ml/head/day in the watering of lactating cows during the increasing the milk yield period there was a tendency to correct metabolic disorders. There was the decrease in the content of ketone bodies by 11,9 %, the increase in glucose by 34 % and the decrease in urea by 7,1 mmol/l.

Study of the Influence of Local Strains of Lactobacillus on the Experimental Model of Ulcerative Colitis

The article is devoted to one of the most important effects of local strains of lactic acid bacteria - the detection of anti-inflammatory action. For this purpose, studies were conducted on an experimental model of ulcerative colitis caused in experimental animals (mice). The data proving that the effect of various probiotic bacteria is unequal are presented. The ability of probiotic lactic acid bacteria in vitro and in vivo systems to influence the production of pro - and anti-inflammatory cytokines, to stimulate antimicrobial, anti-inflammatory, and protective effects have been shown. It is shown that it is necessary to study the specific effect of local lactobacillus strains on anti-inflammatory properties for a more adequate, effective selection of probiotic drugs in the treatment of intestinal ulcers. Data have been presented proving the positive effects of probiotic bacteria on the wound key.

SUBCHRONIC TOXICITY OF MICROENCAPSULATED PROBIOTIC DRUG “ENZYMSPORINE WITH TRYPSIN” AND ITS APPLICATION IN PIG PRODUCTION

The rationale for the study is explained by the fact that regular application of probiotic feeding supplements in pig breeding positively influences the growth and development of young stock, in particular, increases mean daily weight gain, normalizes the performance of the gastrointestinal tract and enforces the resistance of the organism. For this reason, a microencapsulated probiotic drug ―Enzymsporine withtrypsin‖ was developed in the facilities of Kursk State Agricultural Academy (Kursk, Russia). The authors studied the drug’s biological safety. The main method of the study of biological safety of the drug was the study of its subchronic toxicity that would allow for the production trials of this drug on agricultural animals. The article presents the experimental data obtained during the tests on Wistar rats.The tests on rats showed that new encapsulated probiotic drug ―Enzymsporine with trypsin‖ was safe in the doses that exceeded the recommended for the application in animal farming by 2 and 10 times. This provided perspectives for the application of this drug in animal production for the optimization of metabolism, an increase in the mass of animals, and the safety of meat products for humans.

ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ ПРОБИОТИЧЕСКИХ ПРЕПАРАТОВ ПРИ САЛЬМОНЕЛЛЕЗЕ ЦЫПЛЯТ-БРОЙЛЕРОВ

We conducted experiments on the prophylactic and therapeutic effectiveness of the drugs Monosporin, Prolam and Bacell in the experimental infection of broiler chickens Salmonella enter-itidis. As a result, it was found that the use of probiotic agents Monosporin, Prolam and Bacell pre-vents the incidence of broiler chickens with salmonellosis of 66.7 %. The use of an antibacterial drug against the background of the use of probiotic agents in-creases the therapeutic effectiveness to 80 %, while when using only one antibiotic, its therapeutic effect was 57 %, which is 3 % lower compared to the group where only probiotic drugs were used.

Влияние пробиотического штамма Bifidobacterium longum на содержание химических элементов в биологических тканях цыплят-бройлеров при минералдефицитной диете

Abstract. The study is aimed at studying the effect of the probiotic strain Bifidobacterium longum on the content of chemical elements in the biological tissues of broiler chickens against the background of a mineral-deficient diet. Methods. The research was conducted on cross Arbor-Icers broiler chickens between the ages of 7 and 42 days old. From the 21-day-old age of chickens, two groups of 15 heads each were formed by the method of analogs, the studied bird was kept on a mineral-deficient diet. Poultry of the experimental group received a liquid probiotic preparation “Soy-bifidum” in a dosage of 0.7 ml/kg of feed. Features of the exchange of chemical elements were determined by studying the content of the elemental composition of the liver, thoracic and femoral muscles on 25 indicators. Results and practical significance. The additional inclusion of the probiotic strain in the diet contributes to the maximum elimination of toxic elements, thereby reducing the intoxication of the entire body. Thus, the probiotic strain Bifidobacterium longum contributed to a significant decrease of aluminium in the liver by on 34 % (p = 0.048), lead – 50 % (p = 0.008), tin – 67 % (p = 0.0064), aluminium in the thoracic muscles by 54.6 % (p = 0.005), and cadmium by 55.6 % (p = 0.005), cadmium in the femoral muscles by 50 % (p = 0.006), lead by 75 % (p = 0.001), strontium – 50 % (p = 0.013) compared to such values in the control group. Scientific novelty. There were described the features of elemental metabolism by 25 indicators of broiler chickens with the introduction of probiotic drugs against the background of a mineral-deficient diet for the first time.

Antagonistic properties and biocompatibility as important principles for development of effective and biosafety probiotic drugs

Antagonistic properties and peculiarities of inter-strain interactions of probiotic microorganisms were investigated. The study of the antagonistic activity of probiotic strains was carried out by the methods of perpendicular strokes (or delayed antagonism) and the diffusion method (block modification) in relation to opportunistic and pathogenic microorganisms. Most probiotic strains had moderate to high antagonistic activity against both gram-positive and gram-negative pathogens. The results obtained allowed us to select the most active strains, namely L. plantarum AS-41, L. acidophilus A-14, B. subtilis S-2 and B. subtilis S-18, which have a high probiotic potential. To assess biocompatibility, the method of co-cultivation of the selected strains on a modified agar MRS medium was used. The strains that showed biocompatibility of the type “mutual neutrality” or “contact progression” were selected to create a consortium of probiotic microorganisms. Further study of the investigated strains of probiotic bacteria opens up the possibilities of their use for the manufacture of a combined preparation for veterinary purposes, for the prevention and treatment of diseases of the gastrointestinal tract of farm animals.

Application of enzyme probiotic drug developed based on microorganisms of the rumen of reindeer (Rangifer tarandus) in feeding cows

In the current conditions of intense technologies of milk production, the feeding of dairy cows is aimed at providing fast rates of growth and maximum productivity over a short period. However, such systems of diets often have negative effects on the health of the animals as a result of metabolism malfunctioning. The present study was conducted to assess the influence of the enzyme probiotic preparation Rumit, developed based on cellulolytic bacteria isolated from the rumen of reindeer, on dairy activity, food intake, density of ciliate fauna of the rumen, feeding activity, and also metabolic profile and the level of natural resistance of newly-calved heifers. To conduct the experiment, we composed two groups (control and experimental) of first-calf heifers with 12 individuals in their first 100 days of lactation after calving. The first-calf heifers of the experimental group, in addition to the main diet, received the preparation in the amount of 50 g per individual daily in the lunch time feeding for 90 days. Inclusion of the probiotic increased the dairy productivity, particularly a 3.1 kg increase in the average yield of daily 4%-fat milk, 9.6 kg in the butterfat output, and 7.7 kg in that of dairy protein. With increase in dairy productivity, a 0.09 EFU (energy fodder units, equals 10 MJ) decrease was observed in energy expenditure for 1 kg of milk. Intake of the probiotic led to increase in the index of nutritional activity by 0.03 units and increase in the density of ciliates by 155, 900 individuals/mL. Use of the biopreparation had a positive effect on the metabolic processes in the organism of heifers. The animals of the experimental group were observed to have an increase in the content of the total protein in the blood, 12.5% decrease in the urea, and glucose concentration reached the normative values. In the first-calf heifers that had received the food supplement in addition to their diet, the physiological parameters of health normalized and the protective abilities of the organism activated, the pulse and respiration frequencies increased (by 3.8% and 6.6%), the phagocytic index grew by 30.2%, and the absorptive ability of neutrophils increased by 40%. Thus, enzyme probiotic preparation Rumit confirmed its efficiency when fed to newly-calved heifers.

Application efficiency of probiotics in poultry

Due to the growing demand for high-quality poultry and eggs in Kazakhstan, the transition to eco-friendly production is more urgent than ever. The use of probiotics in poultry farms can ensure product safety and economic efficiency. This article presents data on the effectiveness of probiotic drugs used in the world and in domestic poultry farming. It is proved that the introduction of probiotics improves the quality of meat, eggs, and poultry carcass, increases immunity and resistance to antibiotics of 1.5-2% concentration, improves feed conversion by 5-7%, and increases the total body weight from 1.1% to 3.9% compared to control groups. The practical value of this research is to study the market of foreign probiotics used in Kazakhstan poultry farms and study their effectiveness.

Роль лактобактерий в восстановлении нормальной микробиоты влагалища

Achievements of modern science in the field of clinical microbiology, immunology and genetics help to take a different view of the significance of microflora for the human body on the whole. The role of various microorganisms in maintaining the normalization of the female reproductive system is constantly discussed in world literature. The emergence of the method of mass spectrometry has made it possible to identify several hundred types of microorganisms and determine the dominant. Vaginal microbiota is the first line of protection against pathogenic microorganisms in the genital tract. Changes in the content of healthy vaginal microbiome can affect vaginal homeostasis, reducing protective capabilities of the body and thereby increasing women’s susceptibility to infections of the lower genital tract. In addition, the vaginal microbiota plays an important role during pregnancy and childbirth, forming the neonatal microbiome. Despite the importance of studying the patterns of dominance of various types of lactobacilli in the vaginal biotope, there is currently little evidence of their protective function in relation to the female reproductive tract, which remains the subject of study. This knowledge gap is a challenge to the development of effective and applicable treatment methods with probiotic drugs, followed by an assessment of their effectiveness to protect and improve the reproductive health of the female population. Ke ywords: bacterial vaginosis, pregnancy, vaginal probiotics, vaginal microbiome, lactobacillus species identification, lactobacilli, lactic acid

Влияние пробиотического препарата в комплексе с хитозаном на продуктивность молодняка крупного рогатого скота

It has been established that the 2–2,5-month-old calves of 7 g/gol/day probiotic drug "Mucinol" extra together with chitosan for 11 days was sufficient to increase the average daily increase in live mass. The fall of the drug for 21 days did not have a significant impact on the gross and average daily increase in live weight, compared to 11 daily use of this drug.

Development of the composition of feed additives based on the developed algorithm, including the studied probiotic strains

The aim of the study was to develop an algorithm for compounding feed mixtures with the inclusion of probiotic strains. The authors have developed the basic principles underlying the modeling of feed additive formulations. However, the introduction of viable cells of beneficial microorganisms (probiotics) into the feed significantly increases the requirements for the level of design of feed products. Thus, the article describes the basic principles of the algorithm for designing feed products for poultry. A technological scheme for combining the components of the supplement and probiotic drugs has been developed. In accordance with the developed algorithm, the effect of such an active component of the feed additive as bentonite on the growth and survival of the target probiotic strains was tested. Feed mixtures with the inclusion of the probiotic strains under study were tested in the experiment on broiler chickens. The result of the study was positive results of the productivity of the raised poultry in the experimental groups.