Real-time Probe Research Articles

Predictors of Cell-Associated Human Immunodeficiency Virus (HIV)-1 DNA Over 1 Year in Very Early Treated Infants

Younger age of antiretroviral therapy (ART) initiation is associated with smaller viral reservoirs in perinatally acquired HIV-1 infection, but there is wide variability among early-treated infants. Predictors of this variability are not fully described. Sixty-three neonates diagnosed with HIV-1 <48 hours after birth in Johannesburg, South Africa, were started on ART as soon as possible. Fifty-nine (94%) infants received nevirapine prophylaxis from birth until ART start. Viably preserved peripheral blood mononuclear cells (PBMCs) collected at regular intervals to 48 weeks, and from mothers at enrollment, were tested using integrase-targeted, semi-nested, real-time quantitative hydrolysis probe (TaqMan) PCR assays to quantify total HIV-1 subtype C viral DNA (vDNA). Predictors were investigated using generalized estimating equation regression. Thirty-one (49.2%) infants initiated ART <48 hours, 24 (38.1%) <14 days, and 8 (12.7%) >14 days of birth. Three-quarters were infected despite maternal antenatal ART (however, only 9.5% of women had undetectable viral load closest to delivery) and 86% were breastfed. Higher infant CD4+ T-cell percentage and viral load <100 000 copies/mL pre-ART were associated with lower vDNA in the first 48 weeks after ART start. No antenatal maternal ART and breastfeeding were also associated with lower vDNA. Older age at ART initiation had a discernible negative impact when initiated >14 days. Among very early treated infants, higher CD4+ T-cell percentage and viral load <100 000 copies/mL pre-ART, infection occurring in the absence of maternal antenatal ART, and breastfeeding were associated with lower levels of HIV-1 DNA in the first 48 weeks of treatment. Clinical Trials Registration. clinicaltrials.gov (NCT02431975).

Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology.

Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as definitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identification of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox = 46, crab-eating fox = 55) and feces (pampas fox = 84, crab-eating fox = 2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplification of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel real-time PCR TaqMan™ probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the first report of N. caninum DNA in pampas fox feces (n = 1), thus it was also detected from pampas fox tissues (n = 1). Meanwhile, T. gondii was found in tissues of pampas (n = 1) and crab-eating (n = 1) foxes and H. triffittae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.

Investigation of dual plasmonic core-shell Ag@CuS nanoparticles for potential surface-enhanced Raman spectroscopy-guided photothermal therapy.

Aim: To prepare efficient metal-semiconductor nanoparticles as noninvasive, real-time imaging probes for photothermal therapy (PTT) applications. Materials & methods: A bottom-up approach was used to fabricate core-shell Ag@CuS nanoparticles (NPs). PTT and Raman mapping were done using HeLa cells. Theoretical simulation of electric field enhancement and heat dissipation density of Ag@CuS NPs was performed. Results: PTT-induced hyperthermia was achieved under 940nm near-infraredlight irradiation. Surface-enhanced Raman spectroscopy (SERS) signals of dye molecules were observed when conjugated with Ag@CuS NPs. Conclusion: Ag@CuS NPs are found to be efficient for SERS imaging and localized heating under laser irradiation, making a promising candidate for SERS-guided PTT.

Analysis and forecasting of global real time RT-PCR primers and probes for SARS-CoV-2

Rapid tests for active SARS-CoV-2 infections rely on reverse transcription polymerase chain reaction (RT-PCR). RT-PCR uses reverse transcription of RNA into complementary DNA (cDNA) and amplification of specific DNA (primer and probe) targets using polymerase chain reaction (PCR). The technology makes rapid and specific identification of the virus possible based on sequence homology of nucleic acid sequence and is much faster than tissue culture or animal cell models. However the technique can lose sensitivity over time as the virus evolves and the target sequences diverge from the selective primer sequences. Different primer sequences have been adopted in different geographic regions. As we rely on these existing RT-PCR primers to track and manage the spread of the Coronavirus, it is imperative to understand how SARS-CoV-2 mutations, over time and geographically, diverge from existing primers used today. In this study, we analyze the performance of the SARS-CoV-2 primers in use today by measuring the number of mismatches between primer sequence and genome targets over time and spatially. We find that there is a growing number of mismatches, an increase by 2% per month, as well as a high specificity of virus based on geographic location.

Association of Polymorphic and Haplotype Variants of the MSX1 Gene and the Impacted Teeth Phenomenon.

It is known that genetic factors determine odontogenesis; furthermore, studies have revealed that various genes in humans can regulate the development of different types and generations of teeth. In this study it has been assumed that tooth impaction—at least to some extent—also depends on the presence of specific genetic markers, especially allelic variants of the MSX1 gene. The primary objective of the study was to evaluate the suitability of selected molecular markers located within the MSX1 gene for the determination of the risk of tooth impaction in particular patients. The study participants were divided into two groups: (1) the study group—at least one secondary tooth was impacted in the jaws; (2) the control group—no impacted tooth in the jaws. Real-Time PCR and TaqMan probes were used to detect selected polymorphisms in the analyzed genes. Two single nucleotide polymorphisms of MSX1 were analyzed. After the two subgroups of patients were distinguished in the study group based on the number of impacted teeth, statistically significant differences in the frequency of genotypes described for rs12532 in the MSX1 gene were found.

Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

BackgroundVolunteer infection studies have become a standard model for evaluating drug efficacy against Plasmodium infections. Molecular techniques such as qPCR are used in these studies due to their ability to provide robust and accurate estimates of parasitaemia at increased sensitivity compared to microscopy. The validity and reliability of assays need to be ensured when used to evaluate the efficacy of candidate drugs in clinical trials.MethodsA previously described 18S rRNA gene qPCR assay for quantifying Plasmodium falciparum in blood samples was evaluated. Assay performance characteristics including analytical sensitivity, reportable range, precision, accuracy and specificity were assessed using experimental data and data compiled from phase 1 volunteer infection studies conducted between 2013 and 2019. Guidelines for validation of laboratory-developed molecular assays were followed.ResultsThe reportable range was 1.50 to 6.50 log10 parasites/mL with a limit of detection of 2.045 log10 parasites/mL of whole blood based on a parasite diluted standard series over this range. The assay was highly reproducible with minimal intra-assay (SD = 0.456 quantification cycle (Cq) units [0.137 log10 parasites/mL] over 21 replicates) and inter-assay (SD = 0.604 Cq units [0.182 log10 parasites/mL] over 786 qPCR runs) variability. Through an external quality assurance program, the QIMR assay was shown to generate accurate results (quantitative bias + 0.019 log10 parasites/mL against nominal values). Specificity was 100% after assessing 164 parasite-free human blood samples.ConclusionsThe 18S rRNA gene qPCR assay is specific and highly reproducible and can provide reliable and accurate parasite quantification. The assay is considered fit for use in evaluating drug efficacy in malaria clinical trials.

Robotic Intra-Operative Ultrasound: Virtual Environments and Parallel Systems

Robotic intra-operative ultrasound has the potential to improve the conventional practice of diagnosis and procedure guidance that are currently performed manually. Working towards automatic or semi-automatic ultrasound, being able to define ultrasound views and the corresponding probe poses via intelligent approaches become crucial. Based on the concept of parallel system which incorporates the ingredients of artificial systems, computational experiments, and parallel execution, this paper utilized a recent developed robotic trans-esophageal ultrasound system as the study object to explore the method for developing the corresponding virtual environments and present the potential applications of such systems. The proposed virtual system includes the use of 3D slicer as the main workspace and graphic user interface (GUI), Matlab engine to provide robotic control algorithms and customized functions, and PLUS (Public software Library for UltraSound imaging research) toolkit to generate simulated ultrasound images. Detailed implementation methods were presented and the proposed features of the system were explained. Based on this virtual system, example uses and case studies were presented to demonstrate its capabilities when used together with the physical TEE robot. This includes standard view definition and customized view optimization for pre-planning and navigation, as well as robotic control algorithm evaluations to facilitate real-time automatic probe pose adjustments. To conclude, the proposed virtual system would be a powerful tool to facilitate the further developments and clinical uses of the robotic intra-operative ultrasound systems.

Developing real-time mechanochromic probes for polymeric materials

Stress sensing in polymeric materials is of fundamental and technological importance and will be greatly advanced through the development of sensitive molecular probes for bond scission. In this issue of <i>Chem</i>, Moore and collaborators report a new type of regioisomer-specific mechanochromophore with a fast and reversible response in polymeric materials, allowing <i>in situ</i> stress probing.

Combined autofluorescence and diffuse reflectance for brain tumour surgical guidance: initial ex vivo study results.

This ex vivo study was conducted to assess the potential of using a fibre optic probe system based on autofluorescence and diffuse reflectance for tissue differentiation in the brain. A total of 180 optical measurements were acquired from 28 brain specimens (five patients) with eight excitation and emission wavelengths spanning from 300 to 700 nm. Partial least square-linear discriminant analysis (PLS-LDA) was used for tissue discrimination. Leave-one-out cross validation (LOOCV) was then used to evaluate the performance of the classification model. Grey matter was differentiated from tumour tissue with sensitivity of 89.3% and specificity of 92.5%. The variable importance in projection (VIP) derived from the PLS regression was applied to wavelengths selection, and identified the biochemical sources of the detected signals. The initial results of the study were promising and point the way towards a cost-effective, miniaturized hand-held probe for real time and label-free surgical guidance.

Failure Mechanisms in DNA Self-Assembly: Barriers to Single-Fold Yield.

Understanding the folding process of DNA origami is a critical stepping stone to the broader implementation of nucleic acid nanofabrication technology but is notably nontrivial. Origami are formed by several hundred cooperative hybridization events-folds-between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples. Individual events are difficult to detect. Here, we present a real-time probe of the unit operation of origami assembly, a single fold, across the scaffold as a function of hybridization domain separation-fold distance-and staple/scaffold ratio. This approach to the folding problem elucidates a predicted but previously unobserved blocked state that acts as a limit on yield for single folds, which may manifest as a barrier in whole origami assembly.

Critical evaluation of ambient mass spectrometry coupled with chemometrics for the early detection of adulteration scenarios in Origanum vulgare L

Nowadays, most of the screening methods in food manufacturing are based on spectroscopic techniques. Ambient Mass Spectrometry is a relatively new field of analytical chemistry which has proven to offer similar speed and ease-of-use when compared to other fingerprinting techniques, alongside the advantages of good selectivity, sensitivity and chemical information. Numerous applications have been explored in food authenticity, based either on the target detection of adulteration markers or, less frequently, on the development of multivariate classification models. The aim of the present work was to evaluate and compare the capabilities of Direct Analysis in Real Time (DART) and Atmospheric Solid Analysis Probe (ASAP) Mass Spectrometry (MS) for the high-throughput authenticity screening of commercial herbs and spices products. The gross addition of bulking material to dried Mediterranean oregano was taken as case study. First, a pilot sample set, constituted by authentic dried oregano, olive leaves (a frequently reported adulterant) and mixtures thereof at different levels (i.e. 10, 20, 30 and 50% w/w) was used. Each sample was fingerprinted by both ambient-MS techniques. After appropriate pre-processing, the whole mass spectra were used for the subsequent multivariate data analysis. Soft Independent Modelling of Class Analogy was adopted as classification algorithm and the model was challenged with both new authentic oregano and in-house prepared blends. To the best of our knowledge, this is the first report of DART-MS and ASAP-MS used in full scan mode and coupled to chemometric modelling as rapid fingerprinting approach for food authentication. Although both the techniques provided satisfactory results, ASAP-MS clearly showed greater potential, leading to reproducible, diagnostic feature-rich mass spectra. For this reason, ASAP-MS was further tested under a more convoluted scenario, where the training and validation sets were enlarged with additional authentic oregano samples and a wider range of adulterant species, respectively. Overall good results were achieved, with 93% model predictive accuracy, and screening detection capability estimated between 5−20% (w/w) addition, depending on the adulterant considered with the only exception of majorana. Investigation of Q residuals could highlight the statistically-relevant chemical markers which could be tentatively annotated by coupling the ASAP probe with a high resolution mass analyser. The results from the validation study confirmed the great potential of ASAP-MS in combination with chemometrics as fast MS-based screening solution and demonstrated its feasibility for classification model building.

Activatable Polymeric Nanoprobe for Near-Infrared Fluorescence and Photoacoustic Imaging of T Lymphocytes.

Development of real-time non-invasive imaging probes to assess infiltration and activation of cytotoxic T cells (CTLs) is critical to predict the efficacy of cancer immunotherapy, which however remains challenging. Reported here is an activatable semiconducting polymer nanoprobe (SPNP) for near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging of a biomarker (granzyme B) associated with activation of CTLs. SPNP comprises a semiconducting polymer (SP) conjugated with a granzyme B cleavable and dye-labeled peptide as the side chain, both of which emit NIRF and PA signals. After systemic administration, SPNP passively targets the tumor and in situ reacts with granzyme B to release the dye-labeled peptide, leading to decreased NIRF and PA signals from the dye but unchanged signals from the polymer. Such ratiometric NIRF and PA signals of SPNP correlate well with the expression level of granzyme B and intratumoral population of CTLs. Thus, this study not only presents the first PA probes for in vivo imaging of immune activation but also provides a molecular design strategy that can be generalized for molecular imaging of other immune-related biomarkers.

Machine Learning Framework for Improving Accuracy of Probe Speed Data

AbstractA tremendous potential exists for using probe data to support various traffic operations activities. However, limited real-time probe data, especially on arterial roads, have become a barri...

Real-time intraoperative functioning lumen imaging probe during endoscopic per-oral pyloromyotomy (pop)

BackgroundEndoscopic per-oral pyloromyotomy (POP) has emerged as a safe and effective first line option in medically refractory gastroparesis. Determining the appropriate extent of the pyloromyotomy continues to present a challenge as there are no standardized tools for measuring changes in pyloric distensibility during the procedure. The objective of this study was to evaluate the utility of using impedance planimetry with endoscopic functional luminal imaging probe (FLIP) to measure changes in pyloric distensibility after POP, and to compare these changes with improvement in symptoms and objective gastric emptying.MethodsPatients with medically refractory gastroparesis underwent POP with FLIP measurements of the pylorus (EndoFLIP®, Medtronic, Fridley MN). FLIP measurements, as well as changes in symptoms measured by the validated gastroparesis cardinal symptom index (GCSI) and scintigraphic gastric emptying studies (GES), were evaluated before and after POP.ResultsA total of 14 patients underwent measurement with FLIP during POP, 12 of whom had pre- and post-POP measurements. Mean pyloric diameter increased by 1.4 mm, from 13.9 mm to 15.3 mm (p = 0.0012). Mean distensibility index increased from 6.2 mm2/mmHg to 9.1 mm2/mmHg (p = 0.0074). Successful division of the pylorus was achieved in 100% of patients with a mean operative time of 36 min and no perioperative complications. The mean length of stay was 0.7 days (0–3 days). Post-POP mean GCSI score improved from 2.97 to 2.28 at a mean follow-up time of 27 days (p < 0.001). Objective improvement in gastric emptying was observed in 80% of patients with scintigraphic GES, with mean four-hour retention decreasing from 46.3% to 32.4% (p < 0.007).ConclusionsFLIP is a safe and feasible tool to provide objective measurements during POP. Larger cohorts with longer follow-up are required to determine if measured improvements in pyloric diameter and distensibility are predictive of sustained improvements in GCSI and GES.

Cycle-Based Queue Length Estimation for Signalized Intersections Using Sparse Vehicle Trajectory Data

Intersection queue length estimation using high-resolution probe vehicle trajectory data has received increasing attentions in recent years. Existing methods for cycle-based queue length estimation still face the challenge of low and/or unstable estimation accuracies under the condition of sparse vehicle trajectory data, i.e., there is no greater than one vehicle trajectory per cycle on average. To address this challenge, this study proposed a novel approach for cycle-based queue length estimation by fusing real-time and historical probe vehicle trajectory data, through a statistical parameter estimation method, i.e., maximum likelihood estimation (MLE). With known signal timing information, firstly, the historical probe trajectory data are used to acquire the arrival flow rate distribution over the entire study period. Then, a likelihood function of queue length is derived by fully exploiting real-time traffic flow information provided by the queued and non-queued probe vehicles. Finally, the MLE method is adopted to estimate the cycle-based queue lengths with the maximum probability. The proposed approach is verified using both simulation and empirical data. Results indicate that precise estimation for cycle-based queue lengths can be realized based on sparse vehicle trajectory data, while showing superiority to a representative existing method. The proposed method is basically an offline method, but it can also work in an online manner if provided a priori arrival distribution either acquired from historical probe vehicle trajectory data or a theoretical assumption.

Remote Monitoring Digitizes Asset-Integrity Management

This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper SPE 197168, “Digitalize Asset-Integrity Management by Remote Monitoring,” by Mohamed Sahid, ADNOC, prepared for the 2019 Abu Dhabi International Petroleum Exhibition and Conference, Abu Dhabi, 11-14 November. The paper has not been peer reviewed. Monitoring of corrosion in process pipelines has always been of paramount importance in ensuring plant-asset integrity. Similarly, steam traps play an important role in ensuring steam quality and, thus, the integrity of critical assets in the plant. The complete paper discusses these two aspects of monitoring asset integrity - real-time corrosion monitoring and real-time steam-trap monitoring - as implemented by the operator. The authors highlight the importance of digitization by means of implementing wireless technology and making data available in remote work stations in real time. Real-Time Corrosion-Monitoring System Corrosion coupons and electrical resistance probes are among the most-tried and -tested methods to monitor corrosion, but the authors detail shortcomings of these systems, focusing their efforts on the option of using nonintrusive ultrasonic sensors for corrosion monitoring. Fixed ultrasonic thickness (UT) monitoring systems measure a localized thickness of vessel wall or pipe through the use of sound waves. They are the fastest method to measure wall thickness and wall loss reliably. The wall thickness is calculated from the reflection of the ultrasonic signal at both external and internal surfaces. UT systems normally include a transducer and a pulser/receiver. The type of transducer used for this application is the ultrasonic transducer, which can be either piezoelectric or variable-capacitive. The pulser generates short electric pulses of energy at a constant rate, which are converted by the transducer into short, high-frequency ultrasonic sound pulses. These pulses are then directed into the material. Any discontinuation or impurity in the path of the ultrasonic sound wave will be reflected and received by the transducer, transformed into an electric signal, and amplified by the receiver to be projected onto the display (in the case of portable UT instruments). Depending on the intensity shown on the display, information about the impurity or discontinuity, such as size, orientation, and location, can be derived accurately. The shortcomings of using portable UT sensors have been overcome by the introduction of permanent UT sensors, which provide wall-thickness measurement continuously at one location in real time. Because these sensors remain fixed at one location for years, it is possible to analyze corrosion at a single point over time, thus detecting early corrosion onset. Real-Time UT Gauging. The operator installed the real-time corrosion-monitoring system in its offshore associated gas (OAG) unit. A UK-based vendor provided UT sensors along with data-management and -viewing software to support data interpretation. Twenty locations were identified in various plants of the OAG unit on the basis of criticality and previously recorded corrosion levels.

Applications of synchrotron in soil science

Synchrotron radiations (SR) are emerging as a real-time probing tool for the wide range of applied sciences. Since the beginning of 1990s, synchrotron-based techniques have become increasingly employed in various fields of life science. The unique properties of Synchrotron radiations break the limits to characterize the material properties than previous laboratory based techniques. The use of SR in soil sciences also has increased dramatically in the last decade. SR techniques are used to assess soil physical, chemical and biological properties. Besides that SR techniques are also used in soil pollution studies and rhizosphere science. So this paper intends to explain about the instrument synchrotron, its techniques used in soil science and applications in soil science. Furthermore the paper tries to elucidate a few relevant researches in soil science which involves SR techniques.

In Situ Probing of Surface Acoustic Waves by Interfacing with Lanthanide Emitters

AbstractSurface acoustic waves (SAWs) are used in a broad range including electronic systems and various sensors. Especially, SAW‐based microfluidic actuations at microscale represent an effective way with unrivaled precision and technological relevance. However, it remains a daunting challenge to develop a reliable and robust method for in situ and real time probing of the acoustic propagation of SAWs. Approach‐based sophisticated equipment claims large consumption and fussy operation, and is also limited for rapid analysis inside microfluidic channels. Other emerging methods suffer from limitations associated with the reproducibility or the application scenarios. Here, a convenient approach is achieved by interfacing with lanthanide emitters. The coupling between ferroelectric host and lanthanide ions promises effective emission manipulation upon external stimuli induced by the SAWs. The photoluminescence fluctuations of lanthanide emitters reflect their interaction with the strain and piezoelectric fields accompanying the propagating SAWs. The strong correlation between the lanthanide luminescence and the acoustic fields can visualize the resonant frequency, energy flow as well as propagation loss of the propagating SAWs on demand without degrading the SAW‐based functionality. These findings would accelerate the development of SAW‐based devices owing to the added functionality provided by the SAW‐luminescence correlative character.

In Situ and Real-Time Nanoscale Monitoring of Ultra-Thin Metal Film Growth Using Optical and Electrical Diagnostic Tools.

Continued downscaling of functional layers for key enabling devices has prompted the development of characterization tools to probe and dynamically control thin film formation stages and ensure the desired film morphology and functionalities in terms of, e.g., layer surface smoothness or electrical properties. In this work, we review the combined use of in situ and real-time optical (wafer curvature, spectroscopic ellipsometry) and electrical probes for gaining insights into the early growth stages of magnetron-sputter-deposited films. Data are reported for a large variety of metals characterized by different atomic mobilities and interface reactivities. For fcc noble-metal films (Ag, Cu, Pd) exhibiting a pronounced three-dimensional growth on weakly-interacting substrates (SiO2, amorphous carbon (a-C)), wafer curvature, spectroscopic ellipsometry, and resistivity techniques are shown to be complementary in studying the morphological evolution of discontinuous layers, and determining the percolation threshold and the onset of continuous film formation. The influence of growth kinetics (in terms of intrinsic atomic mobility, substrate temperature, deposition rate, deposition flux temporal profile) and the effect of deposited energy (through changes in working pressure or bias voltage) on the various morphological transition thicknesses is critically examined. For bcc transition metals, like Fe and Mo deposited on a-Si, in situ and real-time growth monitoring data exhibit transient features at a critical layer thickness of ~2 nm, which is a fingerprint of an interface-mediated crystalline-to-amorphous phase transition, while such behavior is not observed for Ta films that crystallize into their metastable tetragonal β-Ta allotropic phase. The potential of optical and electrical diagnostic tools is also explored to reveal complex interfacial reactions and their effect on growth of Pd films on a-Si or a-Ge interlayers. For all case studies presented in the article, in situ data are complemented with and benchmarked against ex situ structural and morphological analyses.

Experimental Study of Acoustic Channel Reciprocity in the Shallow Ocean

The theoretical proof of channel reciprocity assumes static propagation medium and boundary conditions. Since the assumption makes little sense in the case of dynamic shallow-ocean environments, it brings up a question: how well does channel reciprocity hold in the shallow ocean? To answer this important question, a sea-going experiment was recently conducted to study the shallow-water channel reciprocity experimentally. In the experiment, two custom-built software-defined acoustic modems were deployed to probe the shared channel from two opposite directions. The channel reciprocity was evaluated by comparing the two probed channels directly. To minimize the effects of channel dynamics, a novel handshaking protocol was developed to synchronize the two-way channel probing in spite of the time asynchronism between the two modems. A set of handshaking signals was also designed to facilitate real-time channel probing while overcoming the limitations posed by half-duplex acoustic modems. Our experimental results have shown that while the shallow-ocean channels experienced in our study are not fully reciprocal in terms of channel impulse responses, they are partially reciprocal in terms of the arrival time of the dominant paths at a high probability. This article provides the details of this experimental study, including its design, setups, execution, and results.