Background. Auto-massage with the use of special rollers is now a popular form of biological regeneration in sport. The aim of the study was to assess the impact of the proposed scheme (methodology) of myofascial auto-release on changes in the pain threshold within selected muscle trigger points in professional basketball players. Material and methods. The research was carried out on 20 basketball players of the 2nd Men's Basketball League. The average age of the respondents was 23.35 ± 4.3 years. The study included 4 training units in one week. Pain sensation threshold was measured using the Pain Test FPX Algometer (Wagner). Measurements were taken on the first and last day of the tests according to the scheme: before and after training, and after rolling. The pain threshold was examined on the following trigger points of the muscles: descending and ascending part of trapezius muscle, levator scapula, rectus femoris muscle, tensor fascia lataeand the medial head of the gastrocnemius muscle. The subjects rolled the areas of the above-mentioned muscles 4 days after training (each time for a total of 10 minutes), according to a standardized schedule. Results and conclusions. Based on the obtained results, it was found that the proposed self-massage regimen performed immediately after basketball training had a significant effect on changes in the pain threshold in some muscle areas. Significant differences were observed on the first day of the study in the descending part of the trapezius muscle and the levator scapulae on the left side (p <0.05). After the entire 4-day rolling session, significant differences were found in the pain threshold on the levator scapulae muscle on both sides of the spine (p = 0.03). An important trend was noted regarding the majority of the examined muscle points, after the self-massage by rolling, the pain threshold increased (muscle pain sensitivity decreased). According to the subjective opinion, the majority of basketball players (90%) consider rolling to be an effective method of post-workout regeneration and see the need for systematic use of this form of auto-massage. According to the vast majority of competitors, the presented method of rolling resulted in an improvement in post-workout regeneration (85%) and a reduction in muscle stiffness (60%). Tissue rolling after exercise seems to be an important complementary approah to biological regeneration in sport.