Privileged Site Research Articles

Near-Infrared Light-Driven CO2 Reduction on Cup-Stacked Carbon Nanotubes.

Carbon nanotubes feature one-dimensional nature of collective excitations, wherein strong confinement of surface plasmons severely hinders the liberation of hot electrons (HEs), posing grand challenges for their utilization in photochemistry. In this study, we prototypically achieved directed HEs flow and extraction in hybrid plasmonic CNN based on cup-stacked carbon nanotubes (CSCNTs), taking advantage of their privileged edge-plane sites. The localized pz electronic states and accessible intersubband plasmon excitations in the near-infrared (NIR) regime stands in striking contrast to the conventional concentric carbon nanotubes, as evidenced by combined photo-induced force microscopy (PiFM) and transient photocurrent response. The hybrid comprising intimately integrated CSCNTs-C3N4 effectively sustains interfacial electronic states and underlies the energy extraction out of plasmonic components. The CNN demonstrates almost near-unity NIR light-driven CO2 reduction to CO with a rate of 1.35 µmol g-1 h-1. This work sheds light on the exploitation of metal-free carbon-based plasmonic nanostructures for photocatalytic applications.

Christian Communities During Russia’s War Against Ukraine: Perception of the Churches’ Aid Involvement Through the Eyes of Poles from Lviv—A Pilot Study

The purpose of this study is to present the perceptions of Poles from Lviv regarding the aid involvement of Christian churches during Russia’s war against Ukraine. Christian churches appear privileged and effective sites of aid delivery in the narratives of respondents. However, this does not mean that they have a monopoly on aid activities. The foundation that underpins these activities is particularly evident in the categories highlighted in this article: the role of clergy in local communities (leaders), personal knowledge of society, church infrastructure, and the churches’ experience gained during the USSR’s persecution of religion. The assistance identified by respondents boils down to material and emotional support, experiencing religious practices, and involvement in direct activity on the war front. The research was based on individual in-depth interviews (IDIs), conducted in December 2023.

Seeing Otherwise: ‘The Least of These’ and Revelation in Jean‐Luc Marion

AbstractIn his familiar essay in Phenomenology and the ‘Theological Turn’, Jean‐François Courtine writes that the ‘cardinal experience’ of revelatory phenomena would undoubtedly be the incarnation. But in its singularity, this experience, he admits, seems to elude phenomenological thought. Against such a judgement, many Christian traditions affirm Christ's ongoing presence in diverse privileged sites, in the eucharist, saints, the Church itself, and, via Christ's own identification with ‘the least of these’ in Matthew's parable, in those who suffer. This essay considers this last possibility of divine manifestation, taking up ‘the least of these’ to reconsider the relationship between finitude and the infinite in Jean‐Luc Marion's phenomenogical account of revelation and saturation. While the heart of Marion's account, both in his words and according to his critics, has primarily concerned revelation's contradiction of finite experience, this essay argues that the development of Marion's own hermeneutics and his attentiveness to paradox and kenosis also reveal the confirmation of finitude at the heart of revelation. Revelation, that is, though it comes from elsewhere, is not other than finitude, but finitude otherwise, finitude's transfiguration.

Cancer Vaccines: Recent Insights and Future Directions

The field of cancer immunotherapy has seen incredible advancements in the past decades. mRNA-based cancer vaccines generating de novo T cell responses, particularly against tumor-specific antigens (TSAs), have demonstrated promising clinical outcomes and overcome diverse challenges. Despite the high potential of neoantigens to provide personalized immunotherapies through their tumor specificity and immunogenicity, challenges related to the scarcity of immunogenic neoepitopes have prompted continuous research towards finding new tumor-associated antigens (TAAs) and broader therapeutic frameworks, which may now learn from the genuine successes obtained with neoantigens. As an example, human endogenous retroviruses (HERVs) have emerged as potential alternatives to tumor neoantigens due to their high tumoral expression and ability to elicit both T cell reactivity and B cell responses associated with the efficacy of existing immunotherapies. This review aims to assess the status and limitations of TSA-directed mRNA cancer vaccines and the lessons that can be derived from these and checkpoint inhibitor studies to guide TAA vaccine development. We expect that shared B cell, CD4 and CD8 T cell antigen presentation will be key to stimulate continuous T cell expansion and efficacy for tumors that do not contain pre-existing tertiary lymphoid structures. When these structures are present in highly mutated tumors, the current checkpoint-based immunotherapies show efficacy even in immune privileged sites, and vaccines may hold the key to broaden efficacy to more tumor types and stages.

‘A Rustling Sound’: Voices of WWII Italian Detainees from The Multilingual Archive of Australia

National archives, libraries, and museums both expose and, directly or indirectly, collude with the military, legal, political, and rhetorical processes of exclusion that tend to obfuscate people’s subjectivities. In this article, official spaces assigned to preserve national memory are placed into dialogue with other archival sites of memory preservation and retrieval of family and community past. By looking at the connections between diverse documents related to Italian-speaking individuals who were prisoners of war and civilian internees in Australia, during World War II, the article investigates how this material can be used to question hegemonic discourses and counters the ‘monolingual paradigm’. Letters, diaries, and memoirs that account captivity and marginalisation during wartime offer cases of polyvocal texts as both creative and privileged sites of speech acts, discursive and material interactions where we can locate spaces of agency, connection and affect.

Localized allergic contact dermatitis on skin flap: Unveiling immune privilege in non-immune privileged sites

Localized allergic contact dermatitis on skin flap: Unveiling immune privilege in non-immune privileged sites

Global Political Logics and Mainstream Discourses on Illness in the Declarations of the State of Exception in the Context of the Covid-19 Pandemic: The Case of the USA, France, and Spain.

At the outbreak of the Covid-19 pandemic, several countries declared "states of exception," that is, authorized legal devices that, in the face of circumstances deemed catastrophic, permit the implementation of extraordinary measures and the temporary suspension of some rights in order to restore the previous state of affairs as soon as possible. This paper offers a comparative textual analysis of the different states of exception declared in the USA, France, and Spain. I argue that these texts constitute a privileged site to explore how prevalent global political logics and mainstream discourses on illness are interwoven. Regarding the global political logics in play, I hold that these declarations constitute an instantiation of democracy's autoimmune character; it attacks itself in order to protect itself. Regarding mainstream discourses on illness, I explore how illness is regarded as a threat to one's self (by something seemingly other) and the notion that therapy must consist of securing the self's triumph over anything seemingly other. This twofold analysis reveals that an aporetic dialectic between self and other-as regards politics and illness-operates in these declarations, most likely because it is, in fact, one and the same dialectic, upon which Western epistemology rests. Furthermore, I suggest that these texts reflect and promote these dominant logics, contributing to shape human relationships around the globe in a certain dangerous way.

Shaping a moral body in family dinner talk: Children's socialization to good manners concerning bodily conduct

The family meal has been extensively investigated as a site for children's acquisition of eating-related behaviors and attitudes, as well as culture-specific rules and assumptions. However, little is known about children's socialization to a constitutive dimension of commensality and even social life: good manners concerning bodily conduct. Drawing on 20th century scholarship on body governmentality and good manners, and building on recent studies on family meal as a socialization site, the article sheds light on this overlooked dimension of family commensality. Based on a corpus of more than 20 h of videorecorded family dinner interactions collected in Italy, and using discourse analysis, the article shows that family mealtime constitutes a relevant arena where parents control their children's conduct through the micro-politics of good manners. By participating in mealtime interactions, children witness and have the chance to acquire the specific cultural principles governing bodily conduct at the table, such as “sitting properly”, “eating with cutlery”, and “chewing with mouth closed”. Yet, they are also socialized to a foundational principle of human sociality: one's own behavior must be self-monitored according to the perspective of the generalized Other. Noticing that forms and contents of contemporary family mealtime talk about good manners are surprisingly similar to those described by Elias in his seminal work on the social history of good manners, the article documents that mealtime still constitutes a privileged cultural site where children are multimodally introduced to morality concerning not only specific table manners, but also more general and overarching assumptions, namely the conception of the body as an entity that should be (self)monitored and shaped according to moral standards.

Normative whiteness in Finnish university education

ABSTRACT As a Nordic welfare state, Finland shares a collective self-image as a forerunner of equality and democracy. Education is seen as a key instrument for social justice, and higher education policy in Finland largely reflects this principle. Still, university education is a site of privilege, where student composition mainly represents individuals from white ethnicities and middle-class backgrounds, who speak one of the national languages as their first language. This is the context in which we ask, ‘How does whiteness “matter” in Finnish university education?’ Drawing on interviews with university students racialised as non-white, our study demonstrates first how the norm of whiteness is manifested both in the official and informal university spaces, thus operating a racialised touchstone for belonging. Second, our study shows how the practices of white ignorance among other students and teaching staff prevent the recognition and dismantling of racialised power relations. Thus, we argue that the process of tackling racial inequalities in university education is still in its infancy in Finland. Changing this situation would require, first and foremost, the critical reflection and dismantling of normative whiteness within the Academy.

Lurking Glares. A Comparative Critique of Latency and Cryptotypes

Based on Rodolfo Sacco’s passionate research on cryptotypes and mute law, the essay aims to propose an onto-aesthetic understanding of latency in law. Inspired by a transdisciplinary way of thinking, the Author elevates comparative law to the privileged site where a critique of the unexpressed can be performed; the degree of figurality of the law can be uncovered and measured; an economy of surplus can be disclosed as the proper segnatorial trait of the material appearances of the law. In this direction, comparative law combines a phenomenology of latency with a hermeneutics of the implicit/implied and becomes the place where it is possible to uncover the communality among plural symbolic universes (literature, arts, music) against the tyranny of disciplinary strategy.

In the Garden of Anthropos

The term ‘planetary garden’ was coined by Gilles Clement to refer to the privileged site of the planetary mixing of species that is managed by humans. In the face of the ongoing environmental collapse, we envision the garden as a new locus for symbiotic attachment and original exchange between human and non-human ecologies. Drawing on the garden metaphor, we discuss the conceptual and ecological impacts of human stewardship of the environment. Recognising ecosystems as changing fields of social and technical interactions, we evaluate how conservation strategies shift in tandem with these changes. We explore the influence of emerging technologies on human understanding of natural ecosystems and on societal approaches to conservation. Envisioning the future, we are mapping out the need for human-centric technologies to foster new forms of agency between humans and their environments. While any technological promise does not come without ethical and technical challenges, we advocate for ecological intelligence (EI), a spatialised human-AI collaboration scheme, as a critical condition for reimagining and upscaling conservation practices in the Anthropocene.

Abstract 2614: GlycoConnect™ ADC toolbox expansion with high DAR technology

Abstract Recently topoisomerase 1 inhibitors have gained significant traction as a cytotoxic payload for ADCs based on the clinical success of camptothecin-based ADCs Enhertu® (DXd) and Trodelvy® (SN-38). In contrast to the majority of other approved ADCs, both Enhertu® and Trodelvy® are feature an average of 7-8 payloads per antibody. Obviously, such a high drug-to-antibody ratio (DAR) effectively delivers more drug to the tumor per internalization event, which is key in driving the clinical success. Previously, we have shown that the native glycan of monoclonal antibodies is a privileged site for attachment of cytotoxic payloads1, for the generation of site-specific and stable ADCs with DARs varying from 1-6, while the therapeutic index is further elevated by a highly polar spacer technology (HydraSpace™)2. The success of GlycoConnect™/HydraSpace™ ADCs is further driven by the use of exatecan as a potent and highly cell-permeable payload (SYNtecan E™). Here we show that GlycoConnect™ technology can be readily extended to generate efficacious and safe DAR8 ADCs. We have developed a modular, non-genetic method, involving three simple process steps from any antibody: (a) enzymatic glycan remodeling, (b) strain-promoted azide-alkyne cycloaddition of a bifunctional, branched spacer comprising one strained alkyne and two orthogonal click tags (e.g. tetrazines), and (c) inverse electron-demand Diels-Alder cycloaddition with a branched linker-drug construct based on BCN and a two cytotoxic payloads. The resulting conjugates, with DAR 6 or 8, are rapidly generated with high homogeneity and with good stability. In addition, HIC profiles of the resulting ADCs indicate small relative retention time as an indication of good PK profile. Excellent in vivo tolerability is demonstrated, while efficacy studies in mice xenograft models show that the DAR8 technology clearly out-performs currently marketed ADCs (including Enhertu®). 1 (a) van Geel et al. Chemoenzymatic Conjugation of Toxic Payloads to the Globally Conserved N-Glycan of Native mAbs Provides Homogeneous and Highly Efficacious Antibody−Drug Conjugates. Bioconj. Chem. 2015, 26, 2233-2242. (b) Wijdeven et al. Enzymatic glycan remodeling-metal free click (GlycoConnect™) provides homogenous antibody-drug conjugates with improved stability and therapeutic index without sequence engineering. MAbs 2022, DOI: 10.1080/19420862.2022.2078466. 2 Verkade et al. A Polar Sulfamide Spacer Significantly Enhances the Manufacturability, Stability, and Therapeutic Index of Antibody-Drug Conjugates. Antibodies 2018, 7, 12, doi:10.3390/antib7010012. Citation Format: Lianne Lelieveldt, Elias Post, Marie Nassiet, Veronique Hendriks, Mick Verhagen, Remon van Geel, Sander van Berkel, Floris van Delft. GlycoConnect™ ADC toolbox expansion with high DAR technology [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2614.

Decidual leukocytes respond to African lineage Zika virus infection with mild anti-inflammatory changes during acute infection in rhesus macaques.

Zika virus (ZIKV) can be vertically transmitted during pregnancy resulting in a range of adverse pregnancy outcomes. The decidua is commonly found to be infected by ZIKV, yet the acute immune response to infection remains understudied in vivo. We hypothesized that in vivo African-lineage ZIKV infection induces a pro-inflammatory response in the decidua. To test this hypothesis, we evaluated the decidua in pregnant rhesus macaques within the first two weeks following infection with an African-lineage ZIKV and compared our findings to gestationally aged-matched controls. Decidual leukocytes were phenotypically evaluated using spectral flow cytometry, and cytokines and chemokines were measured in tissue homogenates from the decidua, placenta, and fetal membranes. The results of this study did not support our hypothesis. Although ZIKV RNA was detected in the decidual tissue samples from all ZIKV infected dams, phenotypic changes in decidual leukocytes and differences in cytokine profiles suggest that the decidua undergoes mild anti-inflammatory changes in response to that infection. Our findings emphasize the immunological state of the gravid uterus as a relatively immune privileged site that prioritizes tolerance of the fetus over mounting a pro-inflammatory response to clear infection.

Alex Goldiș, Literature and Memory Wars. Representations of Communism in Contemporary Romanian Fiction

ABSTRACT The democratic environment in Romania after 1989 witnessed the emergence of an unprecedented number of public discourses engaged with the re-evaluation of communism. In line with recent contributions to cultural memory studies (Aleida Assmann, Astrid Erll), this essay tries to assess the role that fiction played in reshaping the communist past and engaging with the post-communist present. Narratives written by Dan Lungu, Radu Pavel Gheo and Vasile Ernu, some of the most representative writers of the Romanian post-1989 era, are examined against the backdrop of public debates regarding the opposition between democracy and totalitarianism, progression and nostalgia, the ʻcivilized Westʼ and the ʻprovincialʼ East. In reaction to the pressure of ideological discourses, this article argues that literature remains a privileged site where alternative versions of memory can intertwine and negotiate their claims. Literary texts that reshape cultural perceptions fulfil the ʻmnemonic multiperspectivityʼ function of the public discourse with particular focus on recovering the marginal voices that are often disregarded by classic historiography.

Uncanny Times: The Case of Eugene de Kock

ABSTRACT This article focuses specifically on post-apartheid representations of Eugene de Kock, who is seen as one of the bookends to the first, more optimistic, phase of South Africa’s post-apartheid moment. The other bookend, also discussed briefly here, is Nelson Mandela, the country’s first democratically elected president. De Kock was a notorious apartheid-era assassin and commanding officer of Vlakplaas, infamously associated with the extra-judicial torture and execution of anti-apartheid freedom fighters in the 1980s. I isolate this time-frame, from Mandela to de Kock, to comment specifically on what can be seen as a distortion of temporality in South African public life – a kind of disjuncture between time and history, to use David Scott’s formulation (2). I argue that this distortion is characteristic of the post-revolutionary present, not only in South Africa, but in many other parts of the world where a struggle for freedom terminates in a neoliberal order. The figure of the prison is central to this discussion: indeed, the prison is understood as one of the privileged sites where meaning is made and contested in the imagination of a national identity. Also central to this discussion is Pumla Gobodo-Madikizela’s remarkably prescient 2003 book about her encounters with de Kock in the ‘C-Max’ section of Pretoria Central prison, A Human Being Died That Night.

"They don't care about people; they only care about the money": the effects of border enforcement, commodification and migration industries on the mobility of migrants in transit through Mexico.

How does border enforcement affect the mobility of migrants and refugees in countries of transit? What impact does it have on migrants' bodily experiences of mobility and their reliance on actors of the migration industry? While the externalization of borders affects undocumented people by increasing their vulnerability to violence during transit, the impact of the migration regime on the social construction of inequalities in every-day interactions and its relationship to the capacity for mobility has not been studied in depth. This article intends to bridge this gap: based on ethnographic fieldwork I conducted between 2013 and 2019, this article analyzes the relation between immigration enforcement and the mobility strategies of migrants and refugees, particularly women. It focuses on the intertwining of border enforcement and violence and their impact on people's bodily mobility experiences in transit through Mexico along intersecting lines of inequality such as race, class, gender and nationality. First, I analyze how border enforcement contributes to internal bordering, thereby increasing the vulnerability and dependence of migrants on brokers for mobility; second, it looks at the bodily experiences of women in transit and the ways in which internal bordering shapes gendered power hierarchies among actors in the field of mobility. The analysis shows how women negotiate mobility and bodily integrity in social interactions with different actors and how they face constraints resulting from the gendered hierarchies to mobility on routes of transit. Furthermore, it demonstrates how women's bodies have become a privileged site for the construction of a 'body politic' exploitable by others, since border enforcement has contributed to weakening the possibilities of negotiating mobility and bodily integrity in transit.

Kathy Acker’s sex negativity

This essay situates Kathy Acker’s work in the feminist sex wars debate of the 1980s. I suggest that the critique of Acker’s work as a “nihilist version of the personal is political” is not ungrounded but might more usefully be understood as a “sex negativity” that emerges from specific feminist avant-garde literary devices. I discuss Acker’s early texts, “Politics” (1972) and “Stripper Disintegration” (1973) to show how sexuality defines Acker’s esthetic and political project. I consider the (negative) feminist reception of Acker’s work, lay out how Acker was involved in the pornography debate, and I bring Acker’s work into conversation with Andrea Dworkin’s thought. The essay argues that Acker’s pseudo-autobiographical strategies and montage techniques pose a problem for the feminist politicizing of self-knowledge and the genre of autobiography as a privileged site of identity formation and emancipation. In the reordering of materials, by way of replacing, exchanging, and negating, transformation is made possible by the act of rewriting’s capacity to reveal substitutability. Acker’s “nihilist” feminist politics challenge the self-determination and authenticity often assumed in the politicizing of lived experience. I also suggest that “the lesbian” functions as a phantasmatic figure in Acker’s early work to circumvent the subject-object logic of the pornographic imagination. In short, Acker’s early work illuminates the complex relation between sexuality, self-objectification, and the act of writing itself. With Acker’s pseudo-autobiographical texts we can conceive of a sex negativity that is not anti-sex but challenges what Michel Foucault calls the “monarchy of sex” through non-positive affirmation.

187P Local and systemic anti-tumor response during tumor development in an immune privileged site: The case of uveal melanoma

187P Local and systemic anti-tumor response during tumor development in an immune privileged site: The case of uveal melanoma

A comprehensive overview of diagnosis, imaging and treatment of vitreoretinal lymphoma.

Vitreoretinal lymphoma (VRL) is a rare B-cell intraocular neoplasia characterized by poor long-term prognosis and lack of effective therapies. It mainly involves the vitreous humor, the retina, and the retinal pigment epithelium (RPE), although anterior segment involvement can occur. VRL is classified as a lymphoma of immune privileged sites, along with testis lymphoma and primary central nervous system lymphoma (PCNSL). VRL and PCNSL are strictly connected indeed: 80% of VRL develop PCNSL, while 20% of patients with PCNSL present VRL during natural history of lymphoma. Due to the lack of worldwide consensus about diagnosis, therapy, and follow-up timing, VRL represents one of the most challenging ocular affections.VRL commonly masquerades as a posterior uveitis, and misdiagnosis often occurs because of partial response to topical steroids. Gold standard for diagnosis is cytological analysis of vitreous humor. However, this technique lacks sensitivity and supplemental molecular analyses can improve the diagnostic process. Multimodal imaging allows ophthalmologists to empower their clinical suspicion and a comprehensive examination can highlight typical features of VRL and justify further invasive procedures.There is no consensus about VRL therapy, and none of the therapeutical scheme has demonstrated to prevent cerebral involvement and improve patient's overall survival. Intravitreal injections of chemotherapeutics drugs, ocular radiation therapy and systemic chemotherapy can be considered in the treatment of VRL. Once cerebral involvement occurs, systemic chemotherapy must be included in the treatment as a life-saving therapy. Further multicentric studies are required to find out the best treatment of patients with VRL.

Gut Microbiota to Microglia: Microbiome Influences Neurodevelopment in the CNS.

The brain is traditionally viewed as an immunologically privileged site; however, there are known to be multiple resident immune cells that influence the CNS environment and are reactive to extra-CNS signaling. Microglia are an important component of this system, which influences early neurodevelopment in addition to modulating inflammation and regenerative responses to injury and infection. Microglia are influenced by gut microbiome-derived metabolites, both as part of their normal function and potentially in pathological patterns that may induce neurodevelopmental disabilities or behavioral changes. This review aims to summarize the mounting evidence indicating that, not only is the Gut-Brain axis mediated by metabolites and microglia throughout an organism's lifetime, but it is also influenced prenatally by maternal microbiome and diet, which holds implications for both early neuropathology and neurodevelopment.